Salt-adaptive strategies in oil seed crop Ricinus communis early seedlings (cotyledon vs. true leaf) revealed from proteomics analysis.

Soil salinity is a major abiotic stress affecting crop growth and productivity. Ricinus communis has good salt tolerance and is also an important oilseed crop throughout the world. Early seedling stage (such as cotyledon expansion stage) is the most vulnerable period for plant under stresses. However, little information exist concerning the physiological and molecular mechanisms of Ricinus communis seedlings and the role play by cotyledons and true leaf under salt stress. In the present study, biomass, photosynthesis, chlorophyll fluorescence, inorganic ions and organic solutes contents were measured, and two dimensional gel electrophoresis-based proteomic technology was employed to identify the differentially abundant proteins in the salt-treated Ricinus communis cotyledons and true leaves. The results showed that salt stress reduced growth and photosynthesis in the seedlings. With increasing salinity, the Na+ content increased and K+ content decreased in both cotyledons and leaves, but the true leaves had lower Na+ and higher K+ contents. Soluble sugars and proline are the primary organic solutes to cope with osmotic stress. In addition, proteomic analysis revealed 30 and 42 differentially accumulated protein spots in castor cotyledon and true leaf under salt stress, respectively. Most of the identified proteins were involved in carbohydrate and energy metabolism, photosynthesis, genetic information process, reactive oxygen species metabolism, amino acid metabolism and cell structure. The physiological and proteomic results highlighted that cotyledons accumulated a large number of Na+ and provided more energy to help true leaves cope with salt stress. The true leaves saved carbon structures to synthesize osmotic substances, and the enhancement of chlorophyll synthesis and electron transfer in true leaves could also maintain photosynthesis under salt stress. These findings provide new insights into different physiological mechanisms in cotyledon and true leaf of Ricinus communis response to salt stress during early seedling stage.

Rhizomes help the forage grass Leymus chinensis to adapt to the salt and alkali stresses.

Leymus chinensis has extensive ecological adaptability and can grow well in saline-alkaline soils. The knowledge about tolerance mechanisms of L. chinensis could be base for utilization of saline-alkaline soils and grassland restoration and rebuilding. Two neutral salts (NaCl : Na2SO4 = 9 : 1) and two alkaline salts (NaHCO3 : Na2CO3 = 9 : 1) with concentration of 0, 100, and 200 mmol/L were used to treat potted 35-day-old seedlings with rhizome growth, respectively. After 10 days, the biomass and number of daughter shoots all decreased, with more reduction in alkali than in salt stress. The rhizome biomass reduced more than other organs. The number of daughter shoots from rhizome was more than from tillers. Under both stresses, Na(+) contents increased more in rhizome than in other organs; the reduction of K(+) content was more in underground than aerial tissue. Anion ions or organic acids were absorbed to neutralize cations. Na(+) content in stem and leaf increased markedly in high alkalinity (200 mmol/L), with accumulation of soluble sugar and organic acids sharply. Rhizomes help L. chinensis to adapt to saline and low alkaline stresses by transferring Na(+). However, rhizomes lost the ability to prevent Na(+) transport to aerial organs under high alkalinity, which led to severe growth inhibition of L. chinensis.

Spatial and temporal characteristics analysis and prediction model of PM2.5 concentration based on SpatioTemporal-Informer model.

The primary cause of hazy weather is PM2.5, and forecasting PM2.5 concentrations can aid in managing and preventing hazy weather. This paper proposes a novel spatiotemporal prediction model called SpatioTemporal-Informer (ST-Informer) in response to the shortcomings of spatiotemporal prediction models commonly used in studies for long-input series prediction. The ST-Informer model implements parallel computation of long correlations and adds an independent spatiotemporal embedding layer to the original Informer model. The spatiotemporal embedding layer captures the complex dynamic spatiotemporal correlations among the input information. In addition, the ProbSpare Self-Attention mechanism in this model can focus on extracting important contextual information of spatiotemporal data. The ST-Informer model uses weather and air pollutant concentration data from numerous stations as its input data. The outcomes of the trials indicate that (1) The ST-Informer model can sharply capture the peaks and sudden changes in PM2.5 concentrations. (2) Compared to the current models, the ST-Informer model shows better prediction performance while maintaining high-efficiency prediction [Formula: see text]. (3) The ST-Informer model has universal applicability, and the model was applied to the concentration of other pollutants prediction with good results.

Associations between self-disgust, depression, and anxiety: A three-level meta-analytic review.

Self-disgust is a negative self-conscious emotion that reflects disgust or revulsion directed toward oneself. A growing body of research has demonstrated a link between self-disgust, depression, and anxiety. However, the strength of these associations varied considerably across studies, suggesting the need to conduct a meta-analysis to produce a synthesized truer estimate. This review aimed to summarize the primary literature and improve our insight into these associations. The present study used three-level meta-analytic models to synthesize effect sizes and investigate potential moderators of the associations of self-disgust with depression and anxiety. The results revealed a significant association between self-disgust and depression (pooled r = 0.520, 95 % CI [0.485; 0.669], p < .001). The results also showed a significant and moderate association between self-disgust and anxiety (pooled r = 0.452, 95 % CI [0.419; 0.556], p < .001). These associations were held according to sex and age. In conclusion, this meta-analysis supports a moderate-to-large association between self-disgust, depression, and anxiety, suggesting that it is worthy of consideration in research and clinical practice.

Arbuscular mycorrhizal fungi improve the growth and performance in the seedlings of Leymus chinensis under alkali and drought stresses.

Alkali and drought stresses are increasing severe environmental problems throughout the world, especially in the Songnen grassland of northern China. Leymus chinensis is the dominant grass species in the Songnen grassland of northern China and the most promising species for grassland restoration. Arbuscular mycorrhizal fungi (AMF) can colonize 80% of vascular plants, which can enhance the growth of host plants and provide extrinsic protection against abiotic stresses. However, little is known about the interaction effect of alkali and drought stresses on plant-AM symbionts. Here, seedlings of Leymus chinensis inoculated with or without mycorrhizae were cultivated in soil with 0, 100 or 200 mM NaHCO3 under 0, 5 or 10% (w/v) PEG treatment, and the changes in growth, osmotic adjustment substances and ions were measured. The results showed that the interaction of alkali and drought stresses caused greater seedling growth inhibition than either single alkali or drought stress due to ion toxicity and oxidative damage. Mycorrhizae could alleviate the growth inhibition of seedlings under alkali or drought stress. The interaction of alkali and drought stresses did not affect the alleviating effect of mycorrhizae on seedling growth but improved the osmotic regulation ability and ionic balance of the seedlings. Our results clearly show different effects of the interaction of alkali and drought stresses versus a single stress (alkali or drought) on plant development and provide new insights into the positive effect of arbuscular mycorrhizal fungi on host plants under such stress conditions.

[Advances in the mechanism of plant adaptation to acid aluminum stress].

The aluminum stress in acidic soil areas of China is an important abiotic stress factor that hampers the normal growth and development of plants and seriously affects the agricultural yield. The forms of plant resistance to aluminum stress are complex and diverse, which include secretion of organic acids, increase of rhizosphere pH, secretion of mucus, cell wall fixation of Al3+, organic acid chelation of Al3+ in cell solute, and vacuolar area isolation. Most of studies focus on analyzing conventional physiological characteristics, but in-depth molecular biological analyses are lacking. This review summarizes the mechanisms how plants adapt to acidic aluminum stress. This includes the effect of acid aluminum stress on plant growth and physiological metabolism, the two main physiological mechanisms of plant adaptation to acid aluminum stress (aluminum exclusion mechanism, aluminum tolerance mechanism), and the aluminum resistance related genes. Finally, this paper puts forward some prospects for further revealing the mechanism of plant adaptation to acid aluminum stress and excavating high-quality crops suitable for cultivation in acidic soils.

[The physiology of plant seed aging: a review].

Seed quality plays an important role in the agricultural and animal husbandry production, the effective utilization of genetic resources, the conservation of biodiversity and the restoration and reconstruction of plant communities. Seed aging is a common physiological phenomenon during storage. It is a natural irreversible process that occurs and develops along with the extension of seed storage time. It is not only related to the growth, yield and quality of seed and seedling establishment, but also has an important effect on the conservation, utilization and development of plant germplasm resources. The physiological mechanisms of seed aging are complex and diverse. Most studies focus on conventional physiological characterization, while systematic and comprehensive in-depth studies are lacking. Here we review the recent advances in understanding the physiology of seed aging process, including the methods of seed aging, the effect of aging on seed germination, and the physiological and molecular mechanisms of seed aging. The change of multiple physiological parameters, including seed vigor, electrical conductivity, malondialdehyde content and storage material in the seed, antioxidant enzyme activity and mitochondrial structure, were summarized. Moreover, insights into the mechanism of seed aging from the aspects of transcriptome, proteome and aging related gene function were summarized. This study may facilitate the research of seed biology and the conservation and utilization of germplasm resources.

Uniform Water Potential Induced by Salt, Alkali, and Drought Stresses Has Different Impacts on the Seedling of Hordeum jubatum: From Growth, Photosynthesis, and Chlorophyll Fluorescence.

Hordeum jubatum is a halophyte ornamental plant wildly distributed in the Northeast of China, where the low water potential induced by various abiotic stresses is a major factor limiting plant growth and development. However, little is known about the comparative effects of salt, alkali, and drought stresses at uniform water potential on the plants. In the present study, the growth, gas exchange parameters, photosynthetic pigments, and chlorophyll fluorescence in the seedlings of H. jubatum under three low water potentials were measured. The results showed that the growth and photosynthetic parameters under these stresses were all decreased except for carotenoid (Car) with the increasing of stress concentration, and alkali stress caused the most damaging effects on the seedlings. The decreased net photosynthetic rate (Pn), stomatal conductance (Gs), and intercellular CO2 concentrations (Ci) values under salt stress were mainly attributed to stomatal factors, while non-stomatal factors were dominate under drought and alkali stresses. The reduced chlorophyll and slightly increased Car contents occurred under these stresses, and most significant changed under alkali stress. In addition, the maximum photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPSII), and photochemical quenching coefficient (qP) under the stresses were all decreased, indicating that salt, alkali, and drought stresses all increased susceptibility of PSII to photoinhibition, reduced the photosynthetic activity by the declined absorption of light for photochemistry, and increased PSII active reaction centers. Moreover, the non-photochemical quenching coefficient (NPQ) of alkali stress was different from salt and drought stresses, showing that the high pH of alkali stress caused more damaging effects on the photoprotection mechanism depending on the xanthophyll cycle. The above results suggest that the H. jubatum has stronger tolerance of salt than drought and alkali stresses, and the negative effects of alkali stress on the growth and photosynthetic performance of this species was most serious.

Untargeted LC-MS-based metabolomics revealed specific metabolic changes in cotyledons and roots of Ricinus communis during early seedling establishment under salt stress.

Early seedling development is one of the most crucial period of the plant's life cycle, which is highly susceptible to adverse environmental conditions, especially those impose by salt stress. Castor plant (Ricinus communis) is a famous non-edible oilseed and salt-resistant crop worldwide. However, the specific metabolic responses in the cotyledons and roots of this species during seedling establishment under salt stress are still not clearly understood. In the present study, 16 d castor seedlings were treated with 150 mM NaCl for 6 d, and the metabolite profiling of cotyledons and roots was conducted using liquid chromatography (LC) combined with electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS). The Principal Component Analysis (PCA) results showed that the metabolites were great differed in cotyledons and roots under salt stress. There were 38 differential metabolites, mainly including fatty acid, nucleic acid and organic acids in the cotyledons, but only 19 differential metabolites, mainly including fatty acid and organic acids in the roots under such condition. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that flavone and flavonol biosynthesis, pantothenate and CoA biosynthesis, citrate cycle and carotenoid biosynthesis were the common metabolic pathways in response to salt stress in the two organs. Salt stress caused metabolite process alteration mainly on carbon and nitrogen metabolisms, and the carbon allocation from root to cotyledon was increased. Additionally, changes of amino acids and nucleic acids profiles were only found in the cotyledons, and the roots could enhance the activity of antioxidant enzyme systems to scavenge ROS under salinity. In conclusion, the present research provides an improved understanding on specific physiological changes in the cotyledons in castor early seedlings, and explores their interaction under salt stress.

[Advances in the physiological functions of plant lipids in response to stresses].

Lipids are important components of living organisms that participate in and regulate a variety of life activities. Lipids in plants also play important physiological functions in response to a variety of abiotic stresses (e.g. salt stress, drought stress, temperature stress). However, most research on lipids focused on animal cells and medical fields, while the functions of lipids in plants were overlooked. With the rapid development of "omics" technologies and biotechnology, the lipidomics has received much attention in recent years because it can reveal the composition and function of lipids in a deep and comprehensive way. This review summarizes the recent advances in the functions and classification of lipids, the development of lipidomics technology, and the responses of plant lipids against drought stress, salt stress and temperature stress. In addition, challenges and prospects were proposed for future lipidomics research and further exploration of the physiological functions of lipids in plant stress resistance.

Exogenous Abscisic Acid Alleviates Harmful Effect of Salt and Alkali Stresses on Wheat Seedlings.

Exogenous hormones play an important role in plant growth regulation and stress tolerance. However, little is known about the effect of exogenous abscisic acid (ABA) on wheat seedlings under salt and alkali stresses. Here, a pot experiment of saline and alkaline stresses (0 and 100 mmol/L) in which ABA water solution (0, 50 and 100 µmol/L) was sprayed on wheat seedlings was conducted to study the alleviative effectiveness of ABA on salt and alkali stresses. After spraying ABA (50 µmol·L-1), shoot biomass increased 19.0% and 26.7%, respectively. The Na+ content in shoots reduced from 15-fold and 61.5-fold to 10-fold and 37.3-fold in salt and alkali stresses, compared to controls. In addition, proline and organic acid synthesis in shoots also reduced significantly, but the soluble sugar content increased under alkali stress. A high concentration of ABA (100 µmol·L-1) had no significant effects on biomass and ion content in wheat seedlings under both stresses. In conclusion, foliar application of ABA with moderate concentration could effectively accelerate shoot growth of salt-induced wheat seedlings by adjusting the levels of ions and organic solutes.

Metabolomics Analysis Reveals the Alkali Tolerance Mechanism in Puccinellia tenuiflora Plants Inoculated with Arbuscular Mycorrhizal Fungi.

Soil alkalization is a major environmental threat that affects plant distribution and yield in northeastern China. Puccinellia tenuiflora is an alkali-tolerant grass species that is used for salt-alkali grassland restoration. However, little is known about the molecular mechanisms by which arbuscular mycorrhizal fungi (AMF) enhance P. tenuiflora responses to alkali stress. Here, metabolite profiling in P. tenuiflora seedlings with or without arbuscular mycorrhizal fungi (AMF) under alkali stress was conducted using liquid chromatography combined with time-of-flight mass spectrometry (LC/TOF-MS). The results showed that AMF colonization increased seedling biomass under alkali stress. In addition, principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) demonstrated that non-AM and AM seedlings showed different responses under alkali stress. A heat map analysis showed that the levels of 88 metabolites were significantly changed in non-AM seedlings, but those of only 31 metabolites were significantly changed in AM seedlings. Moreover, the levels of a total of 62 metabolites were significantly changed in P. tenuiflora seedlings after AMF inoculation. The results suggested that AMF inoculation significantly increased amino acid, organic acid, flavonoid and sterol contents to improve osmotic adjustment and maintain cell membrane stability under alkali stress. P. tenuiflora seedlings after AMF inoculation produced more plant hormones (salicylic acid and abscisic acid) than the non-AM seedlings, probably to enhance the antioxidant system and facilitate ion balance under stress conditions. In conclusion, these findings provide new insights into the metabolic mechanisms of P. tenuiflora seedlings with arbuscular mycorrhizal fungi under alkali conditions and clarify the role of AM in the molecular regulation of this species under alkali stress.

Changes in photosynthesis and respiratory metabolism of maize seedlings growing under low temperature stress may be regulated by arbuscular mycorrhizal fungi.

Arbuscular mycorrhizal fungi as an important soil microbe have been demonstrated to mitigate the harmful effects of stress on plants. However, little is known about the molecular mechanisms underlying the AM symbiotic response to low temperature. Here, differentially expressed genes (DEGs) in the maize seedlings were identified after inoculating AMF under low temperature conditions. A total of 10,400 DEGs were obtained among four treatments, including non-inoculated AMF under ambient temperature (NMA), inoculated AMF under ambient temperature (MA), non-inoculated with low temperature stress (NML), and inoculated with low temperature stress (ML). The relative expression of 858 genes increased and that of 497 genes decreased in AM plants under low temperature stress. 24 DEGs were identified related to photosynthesis and respiratory metabolism. Among these DEGs, 10 genes were upregulated, and 14 genes were downregulated. The results show that inoculating AMF might decrease the production and transmission of electrons under low temperature, and the cyclic electron flow process in chloroplasts was stimulated to protect plants against low temperature. The fungi also influenced transmission of electrons and production of phosphoric acid in mitochondria in response to low temperature. CO2 assimilation capacity was affected and the tricarboxylic acid cycle was promoted by the adjustments in the glycolysis, pentose phosphate pathway, gamma-aminobutyric acid shunt pathway, and glyoxylic acid cycle to produce more adenosine triphosphate and raw materials for other metabolic pathways under low temperature. These findings provide new insight into low temperature tolerance induced by AMF, and help identify genes for further investigation and functional analyses.

Effects of arbuscular mycorrhizal fungi on Leymus chinensis seedlings under salt-alkali stress and nitrogen deposition conditions: from osmotic adjustment and ion balance.

Nitrogen deposition and soil salinization-alkalization have become major environmental problems throughout the world. Leymus chinensis is the dominant, and considered the most valuable, species for grassland restoration in the Northeast of China. However, little information exists concerning the role of arbuscular mycorrhizal fungi (AMF) in the adaptation of seedlings to the interactive effects of nitrogen and salt-alkali stress, especially from the perspective of osmotic adjustment and ion balance. Experiments were conducted in a greenhouse and Leymus chinensis seedlings were cultivated with NaCl/NaHCO3 under two nitrogen treatments (different concentrations of NH4 +/NO3 -). Root colonization, seedling growth, ion content, and solute accumulation were measured. The results showed that the colonization rate and the dry weights of the seedlings were both decreased with the increasing salt-alkali concentration, and were much lower under alkali stress. Both of the nitrogen treatments decreased the colonization rate and dry weights compared with those of the AM seedlings, especially under the N2 (more NH4 +-N content) treatment. The Na+ content increased but the K+ content decreased under salt-alkali stress, and more markedly under alkali stress. AMF colonization decreased the Na+ content and increased the K+ content to some extent. In addition, the nitrogen treatments had a negative effect on the two ions in the AM seedlings. Under salt stress, the seedlings accumulated abundant Cl- to maintain osmotic and ionic balance, but alkali stress inhibited the absorption of anions and the seedlings accumulated organic acids in order to resist the imbalance of both osmosis and ions, whether under the AM or nitrogen treatments. In addition, proline accumulation is thought to be a typical adaptive feature in both AM and non-AM plants under nitrogen and salt-alkali stress. Our results suggest that the salt-alkali tolerance of Leymus chinensis seedlings is enhanced by association with arbuscular mycorrhizal fungi, and the seedlings can adapt to the nitrogen and salt-alkali conditions by adjusting their osmotic adjustment and ion balance. Excessive nitrogen partly decreased the salt-alkali tolerance of the Leymus chinensis seedlings.

Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China.

Aralia elata buds contain many nutrients and have a pleasant taste with a unique flavour. Previous studies mainly focused on triterpene saponins in the root bark of this species, but little information existed concerning other chemical components, especially in the buds. To better understand the nutritional value of A. elata, we compared total flavonoids, total saponins, phenolic compounds and mineral element contents in the buds of A. elata collected from eight different geographical regions (S1: Benxi; S2: Linjiang; S3: Pingwu; S4: Enshi; S5: Changbaishan; S6: Shangzhi; S7: Xiaoxinganling and S8: Harbin) in China. The results showed that the basic composition in the buds presented a wide variation, with ash (8.76-10.35%), crude fibre (5.38-11.07%), polysaccharides (33.85-46.79 mg g-1), total flavonoid content (TFC, 4.06-48.63 mg g-1) and total saponins (13.62-27.85 mg g-1). UPLC combined with the LC-MS/MS method was used for the phenolic compounds analysis, and 11 phenolic compounds were identified and quantified in the eight samples. The total phenolic content in Enshi (S4) was significantly higher than others, and quercetin was the predominant phenolic compound in this sample. We used ICP-OES to identify and quantify nine mineral elements in the buds. The Fe and Cu contents in S5 were much higher than that of others. We obtained maximum Mg, Mn, Co and Ni contents in S4, and found rich Zn content in S7. Moreover, the maximum estimated quantities of Ca and Sr were found in S8. This study indicated that the chemical composition in the buds of A. elata was obviously affected by geographical origin. Our results provided an essential theoretical basis of quality evaluation of A. elata buds in the food production field.

Germination Responses of Ryegrass (Annual vs. Perennial) Seed to the Interactive Effects of Temperature and Salt-Alkali Stress.

Ryegrass is considered a useful grass species for forage production and turf purposes. Annual ryegrass (Lolium multiflorum Lam.) and perennial ryegrass (Lolium perenne L.)are two species of ryegrass with similar genomes. So far, little information exists concerning their physiological response to salt-alkali stress during germination stage, especially under different temperature regimes. Seeds of ryegrass were germinated at four alternating temperatures (10-20, 15-25, 20-30, and 25-35°C) with salinity (NaCl) and alkalinity (Na2CO3, high pH) stresses. Results showed that optimal germination for both species under stress conditions occurred at higher temperatures (20-30°C for annual ryegrass; 20-30°C and 25-35°C for perennial ryegrass). Germination percentage and germination rate were both inhibited by increasing salinity or alkalinity, particularly higher alkalinities under any temperature. The inhibitory effects of the high salinity on germination were greater at 10-20°C for both species. However, seeds were subjected to more stress at 25-35°C under alkali stress even though the concentration was very low. In addition, both high and low temperatures lead to a markedly decrease in seed germination under alkali stress for perennial ryegrass. Recovery percentage of both species were highest at 400 mM salinity and 25 mM alkalinity under any temperature, especially 10-20°C, and 25-35°C also resulted in lower recovery percentages under both stresses for ryegrass. Moreover, annual ryegrass had a much higher recovery percentage than perennial ryegrass under such stress conditions. These results suggest that salinity stress and alkalinity stress are greatly different, and the salt-alkaline tolerance of ryegrass seeds is greatly affected by the interactions of temperature and salinity-alkalinity.

Physiological Adaptive Strategies of Oil Seed Crop Ricinus communis Early Seedlings (Cotyledon vs. True Leaf) Under Salt and Alkali Stresses: From the Growth, Photosynthesis and Chlorophyll Fluorescence.

Ricinus communis is an important energy crop and is considered as one of the most potential plants for salt-alkali soil improvement in Northeast China. Early seedling stage (such as the cotyledon expansion stage) is always a vulnerable stage but plays a vital role in plant establishment, especially under stress conditions. However, little information exists concerning the function of cotyledon and the relationship between cotyledon and true leaf in the adaptation to salt stress and alkali stress of this species. Here, Ricinus communis seedlings were treated with varying (40, 80 and 120 mM) salinity (NaCl) and alkalinity (NaHCO3), growth, photosynthesis, and chlorophyll fluorescence of cotyledons and true leaves were measured. The results showed that the biomass, photosynthetic parameters, and the qp value of both cotyledons and true leaves decreased with increasing salt-alkali stress, and the decrease in biomass, g s and Tr, of true leaves were much greater than that of cotyledons. Salt-alkali stress only reduced photosynthetic pigments and ΦPSII in cotyledons, but did not affect those in true leaves. Additionally, the Fv/Fm and NPQ between cotyledons and true leaves showed different trends in salinity and alkalinity. The results suggested that alkali stress could cause much more damage to the castor bean seedlings, and different physiological responses and adaptive strategies are found in cotyledons and true leaves under salt-alkali stress. This study will help us develop a better understanding of the adaptation mechanisms of cotyledon and true leaf during early seedling stage of castor bean plant, and also provide new insights into the function of cotyledon in Ricinus communis under salt-alkali stress conditions.

Effects of arbuscular mycorrhizal fungi on the growth, photosynthesis and photosynthetic pigments of Leymus chinensis seedlings under salt-alkali stress and nitrogen deposition.

Leymus chinensis is the most promising grass species for salt-alkaline grassland restoration in northern China. However, little information exists concerning the role of arbuscular mycorrhizal (AM) symbiosis in the adaptation of seedlings to salt-alkali stress, particularly under increased nitrogen deposition, which has become a major environmental problem throughout the world. In this study, Leymus chinensis seedlings were cultivated in soil with 0, 100 and 200mM NaCl/NaHCO3 under two forms of nitrogen (10mM NH4NO3 or NH4Cl: NH4NO3=3:1), and the root colonization, growth and photosynthetic characteristics of the seedlings were measured. The results showed that the colonization rate and intensity decreased with increasing salt-alkali stress and were much lower under alkali stress. The nitrogen treatments also decreased the colonization, particularly under the NH4+-N treatment. Compared with the non-mycorrhizal controls, mycorrhizal seedlings generally presented higher plant biomass, photosynthetic parameters and contents of photosynthetic pigments under stresses, and the inhibitive effects of alkali stress were substantially stronger. In addition, both nitrogen forms decreased the physiological indexes compared with those of the AM seedlings. Our results suggest that salt stress and alkali stress are significantly different and that the salt-alkali tolerance of Leymus chinensis seedlings could be enhanced by associations with arbuscular mycorrhizal fungi, in which would yield better plant growth and photosynthesis. Excessive nitrogen in the soil affects mycorrhizal colonization and thereby inhibits the growth and photosynthetic ability of the seedlings.

Lemmas induce dormancy but help the seed of Leymus chinensis to resist drought and salinity conditions in Northeast China.

Leymus chinensis is a dominant grass in the Songnen grassland of Northern China. The lower germination caused by the presence of lemmas has proved to be an obstacle for the use of the seeds of this plant by humans. However, it is still unknown if the lemmas have other ecological roles such as resisting drought and saline conditions. Three experiments were designed to investigate the ecological roles of the lemmas in Leymus chinensis seeds. The results showed that lemmas significantly improved the amount of water uptake and slowed down the dehydration rate of the seeds under dry conditions. Likewise, the lemmas induced seed dormancy, and removal of the lemmas improved the germination at all temperatures. Although germination percentage of the seeds without lemmas were higher than that of seeds with lemmas under salinity stress, the recovery and total percentage were significantly lower than the seeds with lemmas, especially at 400 mM stress. These results suggest that the lemmas play a vital function in water uptake, dehydration and salt tolerance during the germination stage of the seeds as a response to adverse environmental conditions. Although lemmas showed a dormancy effect, if we want to plant this species in salinity soil in Northeast China, the approach of removing the lemmas by artificial means and improving the seed germination percentage is not feasible.

Optimum harvest maturity for Leymus chinensis seed.

Timely harvest is critical to achieve maximum seed viability and vigour in agricultural production. However, little information exists concerning how to reap the best quality seeds of Leymus chinensis, which is the dominant and most promising grass species in the Songnen Grassland of Northern China. The objective of this study was to investigate and evaluate possible quality indices of the seeds at different days after peak anthesis. Seed quality at different development stages was assessed by the colours of the seed and lemmas, seed weight, moisture content, electrical conductivity of seed leachate and germination indices. Two consecutive years of experimental results showed that the maximum seed quality was recorded at 39 days after peak anthesis. At this date, the colours of the seed and lemmas reached heavy brown and yellow, respectively. The seed weight was highest and the moisture content and the electrical conductivity of seed leachate were lowest. In addition, the seed also reached its maximum germination percentage and energy at this stage, determined using a standard germination test (SGT) and accelerated ageing test (AAT). Thus, Leymus chinensis can be harvested at 39 days after peak anthesis based on the changes in parameters. Colour identification can be used as an additional indicator to provide a more rapid and reliable measure of optimum seed maturity; approximately 10 days after the colour of the lemmas reached yellow and the colour of the seed reached heavy brown, the seed of this species was suitable for harvest.