A leaky human colon model reveals uncoupled apical/basal cytotoxicity in early Clostridioides difficile toxin exposure.

Clostridioides difficile (C. difficile) toxins A (TcdA) and B (TcdB) cause antibiotic-associated colitis in part by disrupting epithelial barrier function. Accurate in vitro models are necessary to detect early toxicity kinetics, investigate disease etiology, and develop preclinical models for new therapies. Properties of cancer cell lines and organoids inherently limit these efforts. We developed adult stem cell-derived monolayers of differentiated human colonic epithelium (hCE) with barrier function, investigated the impact of toxins on apical/basal aspects of monolayers, and evaluated whether a leaky epithelial barrier enhances toxicity. Single-cell RNA-sequencing (scRNAseq) mapped C. difficile-relevant genes to human lineages. Transcriptomics compared hCE to Caco-2, informed timing of in vitro stem cell differentiation, and revealed transcriptional responses to TcdA. Transepithelial electrical resistance (TEER) and fluorescent permeability assays measured cytotoxicity. Contribution of TcdB toxicity was evaluated in a diclofenac-induced leaky gut model. scRNAseq demonstrated broad and variable toxin receptor expression. Absorptive colonocytes in vivo displayed increased toxin receptor, Rho GTPase, and cell junction gene expression. Advanced TcdA toxicity generally decreased cytokine/chemokine and increased tight junction and death receptor genes. Differentiated Caco-2 cells remained immature whereas hCE monolayers were similar to mature colonocytes in vivo. Basal exposure of TcdA/B caused greater toxicity and apoptosis than apical exposure. Apical exposure to toxins was enhanced by diclofenac. Apical/basal toxicities are uncoupled with more rapid onset and increased magnitude postbasal toxin exposure. Leaky junctions enhance toxicity of apical TcdB exposure. hCE monolayers represent a physiologically relevant and sensitive system to evaluate the impact of microbial toxins on gut epithelium.NEW & NOTEWORTHY Novel human colonocyte monolayer cultures, benchmarked by transcriptomics for physiological relevance, detect early cytopathic impacts of Clostridioides difficile toxins TcdA and TcdB. A fluorescent ZO-1 reporter in primary human colonocytes is used to track epithelial barrier disruption in response to TcdA. Basal TcdA/B exposure generally caused more rapid onset and cytotoxicity than apical exposure. Transcriptomics demonstrate changes in tight junction, chemokine, and cytokine receptor gene expression post-TcdA exposure. Diclofenac-induced leaky epithelium enhanced apical exposure toxicity.

A Binocular Vision-Based Crack Detection and Measurement Method Incorporating Semantic Segmentation.

The morphological characteristics of a crack serve as crucial indicators for rating the condition of the concrete bridge components. Previous studies have predominantly employed deep learning techniques for pixel-level crack detection, while occasionally incorporating monocular devices to quantify the crack dimensions. However, the practical implementation of such methods with the assistance of robots or unmanned aerial vehicles (UAVs) is severely hindered due to their restrictions in frontal image acquisition at known distances. To explore a non-contact inspection approach with enhanced flexibility, efficiency and accuracy, a binocular stereo vision-based method incorporating full convolutional network (FCN) is proposed for detecting and measuring cracks. Firstly, our FCN leverages the benefits of the encoder-decoder architecture to enable precise crack segmentation while simultaneously emphasizing edge details at a rate of approximately four pictures per second in a database that is dominated by complex background cracks. The training results demonstrate a precision of 83.85%, a recall of 85.74% and an F1 score of 84.14%. Secondly, the utilization of binocular stereo vision improves the shooting flexibility and streamlines the image acquisition process. Furthermore, the introduction of a central projection scheme achieves reliable three-dimensional (3D) reconstruction of the crack morphology, effectively avoiding mismatches between the two views and providing more comprehensive dimensional depiction for cracks. An experimental test is also conducted on cracked concrete specimens, where the relative measurement error in crack width ranges from -3.9% to 36.0%, indicating the practical feasibility of our proposed method.

Detection of Antibiotic Resistance in Feline-Origin ESBL Escherichia coli from Different Areas of China and the Resistance Elimination of Garlic Oil to Cefquinome on ESBL E. coli.

The development of drug-resistance in the opportunistic pathogen Escherichia coli has become a global public health concern. Due to the share of similar flora between pets and their owners, the detection of pet-origin antibiotic-resistant E. coli is necessary. This study aimed to detect the prevalence of feline-origin ESBL E. coli in China and to explore the resistance elimination effect of garlic oil to cefquinome on ESBL E. coli. Cat fecal samples were collected from animal hospitals. The E. coli isolates were separated and purified by indicator media and polymerase chain reaction (PCR). ESBL genes were detected by PCR and Sanger sequencing. The MICs were determined. The synergistic effect of garlic oil and cefquinome against ESBL E. coli was investigated by checkerboard assays, time-kill and growth curves, drug-resistance curves, PI and NPN staining, and a scanning electronic microscope. A total of 80 E. coli strains were isolated from 101 fecal samples. The rate of ESBL E. coli was 52.5% (42/80). The prevailing ESBL genotypes in China were CTX-M-1, CTX-M-14, and TEM-116. In ESBL E. coli, garlic oil increased the susceptibility to cefquinome with FICIs from 0.2 to 0.7 and enhanced the killing effect of cefquinome with membrane destruction. Resistance to cefquinome decreased with treatment of garlic oil after 15 generations. Our study indicates that ESBL E. coli has been detected in cats kept as pets. The sensitivity of ESBL E. coli to cefquinome was enhanced by garlic oil, indicating that garlic oil may be a potential antibiotic enhancer.

Nonenzymatic Target-Driven DNA Nanomachine for Monitoring Malathion Contamination in Living Cells and Bioaccumulation in Foods.

Intensive applications of toxic malathion pesticides bring a vital threat to the environment and health. Hence, a credible and sensitive strategy is urgently needed for the respective detection of malathion. In this work, an aptamer-based nonenzymatic autonomous DNA walking machine was fabricated for monitoring trace malathion contamination in cells and foods. Along with the machine walking driven by malathion-triggered reaction entropy, multiple fluorescent signal outputs were thermodynamically generated for signal amplification. The proposed stable DNA nanomachine achieved satisfactory results with a detection limit of 81.9 pg L-1 for testing malathion, which could be applied to actual samples including apple juice, paddy water, and paddy soil. Furthermore, the high stability, sensitivity, and biocompatibility of the nanomachine enabled monitoring of the malathion contamination in living cells and bioaccumulation in lettuce without additional purification. Consequently, with these excellent performances, it is strongly anticipated that the DNA walking machine has tremendous potential to be extended to general platforms against pesticides to avoid malathion-contaminated agricultural production for environmental safety and human health.

Glutathione-Responsive Heterogeneous Metal-Organic Framework Hybrids for Photodynamic-Gene Synergetic Cell Apoptosis.

A core-shell heterogeneous metal-organic framework (MOF) hybrid is sequentially designed by a photosensitized porous coordination network (PCN)-typed MOF as core and Cu2+ -centered zeolitic imidazolate framework (ZIF-67) as shell encapsulating cyanine 3-labelled siRNA. The heterogeneous MOF hybrid realized stimulus-responsive photodynamic therapy (PDT) and controllable siRNA delivery through 1 O2 -assistant endosomal escape for imaging-guided photodynamic-gene synergetic theranostics.

Identification, characterization and expression of rice (Oryza sativa) acetyltransferase genes exposed to realistic environmental contamination of mesotrione and fomesafen.

The plant acetyltransferases (ACEs) belong to a super family of proteins that contribute to secondary metabolisms and involve various abiotic and biotic stress responses. However, how rice ACEs respond to toxic agrochemicals is largely unknown. This study demonstrates that 86 and 83 genes coding ACEs in the transcriptome profiling were expressed under mesotrione (MTR) and fomesafen (FSA) exposure, respectively. Of these, 18 and 8 ACE differentially expressed genes (DEGs) were identified in MTR- and FSA-exposed rice transcriptome datasets. Some of the ACE genes were validated by quantitative RT-PCR analysis. Analysis of biochemical properties of ACEs revealed that many genes have various cis-elements and structural domain which may cope with a variety of biotic and abiotic stress responses and detoxification of xenobiotics. Moreover, the ACE activities in rice were induced under MTR and FSA exposure and reached out to the highest value at the 0.1 mg L-1. The ACE activities in the MTR and FSA treated roots were 2.6 and 3.5 fold over the control and those in shoots with MTR and FSA were 4.0 and 26.1 fold over the control, respectively. These results indicate that the ACE-coding genes can respond to the MTR and FSA stress by increasing their transcriptional level, along with the enhanced specific ACE protein activities in rice tissues.

Biodegradation of butachlor in rice intensified by a regulator of OsGT1.

The constant utilization of herbicide butachlor to prevent weeds in agronomic management is leading to its growing accumulation in environment and adverse impact on crop production and food security. Some technologies proposed for butachlor degradation in waters and farmland soils are available, but the catabolic mechanism in crops polluted with butachlor remains unknown. How plants cope with the ecotoxicity of butachlor is not only a fundamental scientific question but is also of critical importance for safe crop production and human health. This study developed a genetically improved rice genotype by overexpressing a novel glycosyltransferase gene named OsGT1 to accelerate removal of butachlor residues in rice crop and its growth environment. Both transcriptional expression and protein activates of OsGT1 are considerably induced under butachlor stress. The growth of the OsGT1 overexpression rice (OsOE) was significantly improved and butachlor-induced cellular damage was greatly attenuated compared to its wild-type (WT). The butachlor concentrations in shoots and roots of the hydroponically grown OsOE plants were reduced by 14.1-30.7 % and 37.8-47.7 %. In particular, the concentrations in the grain of OsOE lines were reduced to 54.6-85.6 % of those in wild-type. Using LC-Q-TOF-HRMS/MS, twenty-three butachlor derivatives including 16 metabolites and 7 conjugations with metabolic pathways were characterized, and it turns out that the OsOE lines accumulated more degradative products than wild-type, implying that more butachlor molecules were intensively catabolized. Taken together, the reduced residues of parent butachlor in rice and its growth media point out that OsGT1 plays a critical role in detoxifying and catabolizing the poisoning chemical in plants and its environment.

The relationship between negative life events and non-suicidal self-injury (NSSI) among Chinese junior high school students: the mediating role of emotions.

BACKGROUND: Adolescent non-suicidal self-injury (NSSI) is common and adolescence is the most common period of first self-injury, and the occurrence of NSSI is influenced by negative life events and emotional symptoms. The mediating role of emotional symptoms in the interaction between negative life events and NSSI has not been carefully investigated yet. METHODS: For middle school students in three schools in a Chinese province, the Adolescents Self-Harm Scale was used to investigate NSSI, the Adolescent Self-Rating Life Events Check List was used to investigate adolescent negative life events, and the Self-Rating Anxiety Scale and Self-Rating Depression Scale were used to assess their emotional symptoms. After the description of general data and the test for differences between groups, the relationship between negative life events, emotional symptoms and NSSI was analyzed using Pearson correlation analysis. Structural equation modeling was used to analyze the mediating role of emotions in negative life events and NSSI. RESULTS: A total of 2376 junior high school students completed this survey, which revealed an annual NSSI prevalence of 37.1% (n = 881) and a higher prevalence of NSSI among girls and rural adolescents. Among adolescents who developed NSSI, 67.4% (N = 594) used multiple means of self-injury. The most common means of self-injury was hair pulling (51.0%), and the most common NSSI purpose and external factors/events were venting bad emotions or feelings (57.5%) and poor academic performance (44.9%), respectively. Negative life events, emotional symptoms and NSSI were positively associated (P < 0.05). Structural equation modeling with negative life events, emotional symptoms and NSSI as variables showed that the model-fit index matched the data well, with RMSEA = 0.073, AGFI = 0.945, GFI = 0.980, CFI = 0.985, NFI = 0.982, TLI = 0.968, IFI = 0.985, and negative life events, emotional symptoms (anxiety, depression) and NSSI all had direct effects with standardized path coefficients of 0.16, 0.19, and 0.23, respectively, with negative life events playing an indirect role in NSSI through emotional symptoms and emotional symptoms playing an incomplete mediating role in negative life events and NSSI. CONCLUSION: The prevalence of NSSI was higher among Chinese junior high school students. Both negative life events and emotional symptoms were direct risk factors for NSSI. In addition, negative life events were also indirect risk factors for NSSI, and emotional symptoms played an incomplete mediating role in the relationship between the effects of negative life events and NSSI. This indicates that the combination of reducing the frequency of negative life events while maintaining individual emotional stability during adolescent development can effectively reduce the prevalence of NSSI in adolescents.

Multifunctional metal-organic framework heterostructures for enhanced cancer therapy.

Metal-organic frameworks (MOFs) are an emerging class of molecular crystalline materials built from metal ions or clusters bridged by organic linkers. By taking advantage of their synthetic tunability and structural regularity, MOFs can hierarchically integrate nanoparticles and/or biomolecules into a single framework to enable multifunctions. The MOF-protected heterostructures not only enhance the catalytic capacity of nanoparticle components but also retain the biological activity of biomolecules in an intracellular microenvironment. Therefore, the multifunctional MOF heterostructures have great advantages over single components in cancer therapy. In this review, we comprehensively summarize the general principle of the design and functional modulation of nanoscaled MOF heterostructures, and biomedical applications in enhanced therapy within the last five years. The functions of MOF heterostructures with a controlled size can be regulated by designing various functional ligands and in situ growth/postmodification of nanoparticles and/or biomolecules. The advances in the application of multifunctional MOF heterostructures are also explored for enhanced cancer therapies involving photodynamic therapy, photothermal therapy, chemotherapy, radiotherapy, immunotherapy, and theranostics. The remaining challenges and future opportunities in this field, in terms of precisely localized assembly, maximizing composite properties, and processing new techniques, are also presented. The introduction of multiple components into one crystalline MOF provides a promising approach to design all-in-one theranostics in clinical treatments.

All-dielectric thin films based on single silicon materials for angle-insensitive structural colors.

Here we demonstrate a red structural color of a multilayer structure made of all silicon-based materials. By using amorphous silicon (a-Si) and silicon dioxide (SiO2) with a large difference in the refractive index, high reflection efficiency can be achieved with only a few layers. The anti-reflection unit composed of top silicon monoxide (SiO) and SiO2 layers can significantly reduce the reflection intensity of the non-target wavelengths to ensure that the device has good color saturation. The selective absorption of SiO and a-Si layers can further improve the color saturation. By reasonably controlling the thickness of the highly absorbing materials, the device has good angular insensitivity at an incident angle of 0°-60°. Furthermore, the angle-insensitive blue and yellow structural colors are also realized based on the all silicon design idea. This scheme is simple in structure and capable of efficient mass production. This method has enormous potential for diverse applications in display, colorful decoration, anti-counterfeiting, and so forth.

Metal-Organic Framework (MOF) Hybrid as a Tandem Catalyst for Enhanced Therapy against Hypoxic Tumor Cells.

Encapsulation of active biomolecules and/or nanoparticles in metal-organic frameworks (MOFs) remains a great challenge in biomedical applications. In this work, through a stepwise in situ growth method, a black phosphorus quantum dot (BQ) and catalase were precisely encapsulated into the inner and outer layers of MOFs, respectively. The integrated MOF system as a tandem catalyst could convert H2 O2 into O2 in MOF-stabilized catalase outer layer, and then O2 was directly injected into MOF-sensitized BQ inner, leading to high quantum yield of singlet oxygen. Upon internalization, the photodynamic therapy efficiency of the MOF system was 8.7-fold greater than that without catalase, showing an enhanced therapeutic effect against hypoxic tumor cells. Furthermore, by coupling with photothermal therapy of BQs, photodynamic-thermal synergistic therapy was realized both in vitro and in vivo.

Effect of childhood trauma on cognitive functions in a sample of Chinese patients with schizophrenia.

In this study, we aimed to determine the influence of various types of childhood trauma (CT) on cognitive functions in Chinese patients presented with schizophrenia. One hundred sixty-two patients were assessed with the Childhood Trauma Questionnaire-Short Form (CTQ-SF) and Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). We investigated the correlations between various types of CT, demographic characteristics, and cognitive functions. Significant negative correlations were observed in physical abuse (PA) and sexual abuse (SA) with the language score (r=-0.190, -0.216, respectively, p<0.05). Similarly, physical neglect (PN) and the total score of CTQ were negatively correlated with the attention score (r=-0.17, -0.206, p<0.05, respectively) as well as the total RBANS score (r=-0.199, -0.223, respectively P<0.05). PN was also negatively correlated with delayed memory (r=-0.167, p<0.05). Regressions analysis indicated significant negative correlations between PN and attention, as well as the cognitive total score (p<0.001). Furthermore, demographic variables (years of education, family income) and clinical characteristics (type of anti-psychotics, duration of illness and times of recurrence) were correlated with cognitive functions. The current study showed that different types of CT could impact specific cognitive functions in Chinese schizophrenia patients. Therefore, we recommend that trauma-focused mental interventions for schizophrenia patients should be developed and routinely offered to patients.

The risk of male adult alcohol dependence: The role of the adverse childhood experiences and ecological executive function.

OBJECTIVE: To explore the association between male adult alcohol dependence and their adverse childhood experiences as well as ecological executive function. METHODS: The questionnaires of Adverse Childhood Experiences (ACEs) and Behavior Rating Inventory of Executive Function-Adult Version (BRIEF-A) were adopted for the assessments of 102 alcohol dependent patients who were diagnosed according to the criteria defined by the International Classification of diseases and related health problems (ICD-10) and 106 healthy volunteers, and the differences between patients and healthy volunteers were analyzed. RESULTS: The percentage of adverse childhood experiences in alcohol dependent patients was significantly higher than that in healthy volunteers (χ(2)=17.28, P<0.01); and the incidences of emotional abuse, physical neglect, violence witness, and substance abuse were significantly higher in alcohol dependent patients than those in healthy volunteers (χ(2)=4.59, 4.46, 10.51, and 44.09 respectively; P<0.05). The ecological executive function analysis showed that the BRIEF total score and scores for each item were all significantly higher in alcohol dependent patients than those of healthy volunteers (P<0.01). CONCLUSIONS: The adult alcohol dependence was associated with their adverse childhood experiences and ecological executive function. Then physical neglect and substance abuse of parents in childhood, and emotional control defect in the ecological executive function showed strong association with adult alcohol dependence.

Reliability and Validity of the Spence Children's Anxiety Scale for Parents in Mainland Chinese Children and Adolescents.

This study examined the psychometric properties of the Spence Children's Anxiety Scale for Parents (SCAS-P) in 1943 father-mother dyads and 1785 students. Results of confirmatory factor analyses for SCAS-P were in favour of the original model with six correlated factors. The internal consistency of SCAS-P was acceptable (α = .63-.91), and the test-retest reliability was acceptable (r = .46-.72). The convergent and divergent validity of SCAS-P was supported by significant correlations with an internalizing subscale to a greater extent than with an externalizing subscale. Congruent validity was supported by significant correlations between father and mother reports (r = .60-.71) and child and parent reports (r = .25-.42). Significant differences between community and clinical samples supported the discriminant validity. Adolescents showed higher anxiety levels than children, and girls showed higher anxiety levels than boys. Our findings suggest that the SCAS-P is a suitable parent instrument to measure child anxiety symptoms in Mainland Chinese children and adolescents.

Transport and Environmental Risks of Propachlor Within the Soil-Plant-Water Phase as Affected by Dissolved Organic Matter as a Nonionic Surfactant.

Propachlor is a widely used acylaniline herbicide for weeding annual gramineous and dicot plants in farmland. As a toxic agricultural chemical, it is overused in crop production and has become one of the most serious environmental pollutants. Yet, understanding the impact of environmental factors on its behavior in farmland soils is critically important for healthy crop production and food safety. In this study, we characterized the effect of dissolved organic matter (DOM) extracted from rice straw on the mobility behavior of propachlor in farmland soil using comprehensive approaches such as the batch equilibrium experiment, soil thin-layer chromatography, soil column leaching, and wheat bioaccumulation with Triton X-100 (TX-100) as a reference surfactant. The application of DOM at 60 and 120 mg DOC L-1 and TX-100 at 96, 192, and 288 mg L-1 reduced the sorption and increased the desorption of propachlor in soil. Freundlich constant K f values (sorption) of propachlor declined with the expansion of DOM and TX-100 concentrations. The addition of DOM and TX-100 increased the mobility of propachlor and the total concentration of propachlor in the leachate of soil columns. The retention factors (R f) were evaluated in the soil thin-layer chromatography as 0.741 and 0.772 (for DOM) and 0.731, 0.763, and 0.791 (for TX-100), all of which were greater than the control (0.710) under the treatment. The application of DOM or TX-100 reduced root growth (biomass) and increased bioaccumulation of propachlor in the roots and shoots of wheat. The outcome of the study can provide important references for the rational use of propachlor and help agronomic management to minimize pesticide contamination in realistic crop production.

Interpretation of ametryn biodegradation in rice based on joint analyses of transcriptome, metabolome and chemo-characterization.

Agrochemicals such as pesticide residues become environmental contaminants due to their ecotoxic risks to plant, animal and human health. Ametryn (AME) is a widely used farmland pesticide and its residues are widespread in soils, surface stream and groundwater. However, its toxicological and degradative mechanisms in plants and food crops are largely unknown. This study comprehensively investigated AME toxicology and degradation mechanisms in a paddy crop. AME was freely absorbed by rice roots, translocated to the above-ground and thus repressed plant elongation, and reduced dry weight and chlorophyll concentration, but increased oxidative injury and subcellular electrolyte permeability. Analysis of the transcriptome and metabolome revealed that exposure to AME evoked global AME-responsive genes and step-wise catabolism of AME. We detected 995 (roots) and 136 (shoots) upregulated and differentially expressed genes (DEGs) in response to AME. Metabolomic profiling revealed that many basal metabolites such as carbohydrates, amino acids, glutathione, hormones and phenylpropanoids involved in AME catabolism were accordingly accumulated in rice. Eight metabolites and twelve conjugates of AME were characterized by HPLC-Q-TOF-HRMS/MS. These AME metabolites and conjugates are closely related to DEGs, differentially accumulated metabolites (DAMs) and activities of antioxidative enzymes. Collectively, our work highlights the specific mechanisms for AME degradative metabolism through Phase I and II reactive pathways (e.g. hydroxylation and dealkylation), with will help develop genetically engineered rice used to bioremediate AME-contaminated paddy soils and minimize AME accumulation rice crops.

Spatial-Temporal Variations in of Soil Conservation Service and Its Influencing Factors under the Background of Ecological Engineering in the Taihang Mountain Area, China.

Soil conservation (SC) plays an important role in maintaining regional land productivity and sustainable development. Ecological engineering (EE) is being implemented in different countries to effectively alleviate the damage to the ecological environment and effectively protect soil and food security. It is important to determine whether or not the SC capacity becomes stronger after the implementation of EE and whether or not EE has a notable impact on SC in different altitude zones. The exploration of the influencing mechanism and identification of the dominate influencing factors in different geographical regions needs to be improved. In this study, the soil conservation services (SCSs) from 1980 to 2020 in the Taihang Mountain area was assessed using the integrated valuation of ecosystem services and trade-offs (InVEST) model, and the spatial and temporal distributions and influencing factors were explored. The results showed the following: (1) the average SCSs exhibited an increasing trend from 1980 to 2020 on the whole, and the rate of increase reached 50.53% during the 41-year period. The rate of increase of the SCSs varied in the different EE implementation regions, and it was significantly higher than that of the entire study area. (2) The spatial distribution of the SCSs was highly heterogeneous, and the high SCS value areas were coincident with the high-altitude areas where forest and grassland occupied a large proportion. The low value areas were mainly located in the hilly zone or some of the basin regions where the proportion of construction land was relatively high. (3) The distribution pattern of the SCSs was the result of multiple factors. The EE intensity had the strongest explanatory power for the SCSs in the hilly zone, explaining 34.63%. The slope was the most critical factor affecting the SCSs in the mid-mountain and sub-alpine zones. The slope and normalized difference vegetation index (NDVI) had the greatest interactions with the other factors in the three altitude zones, especially in the high-altitude regions. The quantitative analysis of the SCSs and the influences of EE and natural factors on the SCSs revealed the heterogeneity in the mountainous areas. These results also provide a scientific basis for the reasonable implementation of EE and sustainable management of SCSs in the Taihang Mountain area.

An All-Arthroscopic Simple Double 360° Lasso Loop Technique for Supraopectoral Biceps Tenodesis.

In many shoulder joint diseases, there is often a combination of long head biceps tendon（LHBT）pathology. Biceps pathology is one of the main causes of shoulder pain, and it is effectively managed with tenodesis. Biceps tenodesis can be performed in a variety of ways with different fixation and at different locations. This article introduces an all-arthroscopic suprapectoral biceps tenodesis technique with a 2-suture anchor. Double 360° Lasso Loop is used to fix the biceps tendon, and only one puncture of the biceps tendon was required, which caused little damage to the tendon and was not easy for the suture to slip and fail.

Acetyltransferase OsACE2 acts as a regulator to reduce the environmental risk of oxyfluorfen to rice production.

The constant use of the pesticide oxyfluorfen (OFF) in farmland contaminates the soil, posing threats to crop growth and human health. To avoid the contamination of food crops with OFF, it is critically important to understand its absorption and degradation mechanisms. In this study, we characterized a new functional locus encoding an acetyltransferase (OsACE2) that can facilitate OFF degradation in rice. OsACE2 was drastically induced by OFF at 0.04-0.2 mg L-1 for 6 days and the rice growth was significantly inhibited. To demonstrate the regulatory role of OsACE2 in resistance to OFF toxicity, we generated OsACE2 overexpression (OE) and knockout mutant using genetic transformation and gene-editing technologies (CRISPR/Cas9). The OE plants grown in the hydroponic medium showed improved growth (plant elongation and biomass), increased chlorophyll content, and reduced OFF-induced oxidative stress. The OsACE2-improved growth phenotypes of rice were attributed to the significantly lower OFF accumulation in OE plants. Conversely, knocking out OsACE2 resulted in compromised growth phenotypes compared to the wild-type (WT). Using LC-LTQ-HRMS/MS, five mono-metabolites and eleven conjugates of OFF were characterized through various canonical pathways, such as hydrolysis, oxidation, reduction, glycosylation, acetylation, malonylation, and interaction with amino acids. These metabolites increased in the OE plants, and five acetylated conjugates were reported for the first time. Collectively, OsACE2 plays a primary role in catabolizing OFF residues in rice through multiple degradation pathways and reducing OFF in its growth environment.

Detoxification and catabolism of mesotrione and fomesafen facilitated by a Phase II reaction acetyltransferase in rice.

INTRODUCTION: The excessive dosage of pesticides required for agronomic reality results in growing contamination of pesticide residues in environment, thus bringing high risks to crop production and human health. OBJECTIVES: This study aims to unveil a novel mechanism for catabolism of two pesticides MTR and FSA facilitated by an uncharacterized Phase II reaction enzyme termed acetyltransferase-1 (ACE1) in rice and to make assessment of its potential for bioremediation to minimize the risks to crop production and food safety. METHODS: We developed genetically improved cultivars overexpressing OsACE1 (OE) and knockout mutant lines by CRISPR-Cas9 technology to identify the MTR and FSA detoxic and metabolic functions and characterized their metabolites and conjugates by HPLC-LTQ-MS/MS. RESULTS: OsACE1 overexpression conferred rice resistance to toxicity of MTR/FSA compared to wild-type, manifested by improved plant elongation and biomass, attenuated cellular injury, and increased chlorophyll accumulation. The OE plants accumulated significantly less parent MTR/FSA and more degradative metabolites, and removed MTR/FSA from their growth medium by 1.38 and 1.61 folds over the wild-type. In contrast, knocking out OsACE1 led to compromised growth fitness and intensified toxic symptoms under MTR/FSA stress and accumulation of more toxic MTR and FSA in rice. The reduced metabolites of MTR and FSA detected in the Cas9 plants suggest the impaired capability of OsACE1 function. CONCLUSIONS: These results signified that OsACE1 expression is required for detoxifying the two poisoning chemicals in rice and plays a critical role in accelerating breakdown of the pesticides mainly through Phase II reaction mechanism pathways.