Modelling reveals endogenous osmotic adaptation of storage tissue water potential as an important driver determining different stem diameter variation patterns in the mangrove species Avicennia marina and Rhizophora stylosa.

BACKGROUND: Stem diameter variations are mainly determined by the radial water transport between xylem and storage tissues. This radial transport results from the water potential difference between these tissues, which is influenced by both hydraulic and carbon related processes. Measurements have shown that when subjected to the same environmental conditions, the co-occurring mangrove species Avicennia marina and Rhizophora stylosa unexpectedly show a totally different pattern in daily stem diameter variation. METHODS: Using in situ measurements of stem diameter variation, stem water potential and sap flow, a mechanistic flow and storage model based on the cohesion-tension theory was applied to assess the differences in osmotic storage water potential between Avicennia marina and Rhizophora stylosa. KEY RESULTS: Both species, subjected to the same environmental conditions, showed a resembling daily pattern in simulated osmotic storage water potential. However, the osmotic storage water potential of R. stylosa started to decrease slightly after that of A. marina in the morning and increased again slightly later in the evening. This small shift in osmotic storage water potential likely underlaid the marked differences in daily stem diameter variation pattern between the two species. CONCLUSIONS: The results show that in addition to environmental dynamics, endogenous changes in the osmotic storage water potential must be taken into account in order to accurately predict stem diameter variations, and hence growth.

Water use patterns of estuarine vegetation in a tidal creek system.

Water availability is a key determinant of the zonation patterns in estuarine vegetation, but water availability and the use of different water sources over space and time are not well understood. We have determined the seasonal water use patterns of riparian vegetation over an estuarine ecotone. Our aim was to investigate how the water use patterns of estuarine vegetation respond to variations in the availability of tidal creek water and rain-derived freshwater. The levels of natural stable isotopes of oxygen and hydrogen were assessed in the stem of the mangrove Avicennia marina (tall and scrub growth forms), Casuarina glauca and Melaleuca quinquenervia that were distributed along transects from river/creek-front towards inland habitats. The isotopic composition of plant tissues and the potential water sources were assessed in both the wet season, when freshwater from rainfall is present, and the dry season, when mangrove trees are expected to be more dependent on tidal water, and when Casuarina and Melaleuca are expected to be dependent on groundwater. Our results indicate that rainwater during the wet season contributes significantly to estuarine vegetation, even to creek-side mangroves which are inundated by tidal creek water daily, and that estuarine vegetation depends primarily on freshwater throughout the year. In contrast, high intertidal scrub mangroves were found to use the greatest proportion of tidal creek water, supplemented by groundwater in the dry season. Contrary to prediction, inland trees C. glauca and M. quinquenervia were found also to rely predominantly on rainwater--even in the dry season. The results of this study reveal a high level of complexity in vegetation water use in estuarine settings.

Direct uptake of canopy rainwater causes turgor-driven growth spurts in the mangrove Avicennia marina.

Mangrove forests depend on a dense structure of sufficiently large trees to fulfil their essential functions as providers of food and wood for animals and people, CO2 sinks and protection from storms. Growth of these forests is known to be dependent on the salinity of soil water, but the influence of foliar uptake of rainwater as a freshwater source, additional to soil water, has hardly been investigated. Under field conditions in Australia, stem diameter variation, sap flow and stem water potential of the grey mangrove (Avicennia marina (Forssk.) Vierh.) were simultaneously measured during alternating dry and rainy periods. We found that sap flow in A. marina was reversed, from canopy to roots, during and shortly after rainfall events. Simultaneously, stem diameters rapidly increased with growth rates up to 70 µm h-1, which is about 25-75 times the normal growth rate reported in temperate trees. A mechanistic tree model was applied to provide evidence that A. marina trees take up water through their leaves, and that this water contributes to turgor-driven stem growth. Our results indicate that direct uptake of freshwater by the canopy during rainfall supports mangrove tree growth and serve as a call to consider this water uptake pathway if we aspire to correctly assess influences of changing rainfall patterns on mangrove tree growth.

Nitrogen sharing and water source partitioning co-occur in estuarine wetlands.

Plant-plant interactions are particularly complex in multi-resource limited environments. The aim of this study was to assess species interactions in estuarine wetlands where both N and fresh water are limited. We combined stable isotope methods and dissimilarity analyses to compare interspecific interactions in N source use and water source use. Both Melaleuca quinquenervia (Cav.) S. T Blake and Avicennia marina (Forssk.) Vierh. had a lower leaf δ15N when they were growing together with the N-fixer Casuarina glauca Sieb. ex Spreng. compared with those trees growing in monospecific stands, but their water isotopes, δ18O and δD, were different from C. glauca. Our results indicate that the N-fixer C. glauca shared their N with co-existing neighbours, either indirectly or directly, but that water sources were partitioned among them. Further analyses showed that M. quinquenervia and C. glauca had lower dissimilarity in N source use but higher dissimilarity in water source use than the C. glauca-A. marina pair, implying that the co-existence between M. quinquenervia and C. glauca is relatively stable. Our results suggest that facilitative interaction and resource partitioning can co-occur in estuarine wetlands, and which could be important in maintaining diversity across resource gradients.

Using electrical resistivity tomography to differentiate sapwood from heartwood: application to conifers.

Estimating sapwood area is one of the main sources of error when upscaling point scale sap flow measurements to whole-tree water use. In this study, the potential use of electrical resistivity tomography (ERT) to determine the sapwood-heartwood (SW-HW) boundary is investigated for Pinus elliottii Engelm var. elliottii × Pinus caribaea Morelet var. hondurensis growing in a subtropical climate. Specifically, this study investigates: (i) how electrical resistivity is correlated to either wood moisture content, or electrolyte concentration, or both, and (ii) how the SW-HW boundary is defined in terms of electrical resistivity. Tree cross-sections at breast height are analysed using ERT before being felled and the cross-section surface sampled for analysis of major electrolyte concentrations, wood moisture content and density. Electrical resistivity tomography results show patterns with high resistivities occurring in the inner part of the cross-section, with much lower values towards the outside. The high-resistivity areas were generally smaller than the low-resistivity areas. A comparison between ERT and actual SW area measured after felling shows a slope of the linear regression close to unity (=0.96) with a large spread of values (R(2) = 0.56) mostly due to uncertainties in ERT. Electrolyte concentrations along sampled radial transects (cardinal directions) generally showed no trend from the centre of the tree to the bark. Wood moisture content and density show comparable trends that could explain the resistivity patterns. While this study indicates the potential for application of ERT for estimating SW area, it shows that there remains a need for refinement in locating the SW-HW boundary (e.g., by improvement of the inversion method, or perhaps electrode density) in order to increase the robustness of the method.