Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat.

The NAC transcription factor (TF) family is one of the largest TF families in plants, which has been widely reported in rice, maize and common wheat. However, the significance of the NAC TF family in wild emmer wheat (Triticum turgidum ssp. dicoccoides) is not yet well understood. In this study, a genome-wide investigation of NAC genes was conducted in the wild emmer genome and 249 NAC family members (TdNACs) were identified. The results showed that all of these genes contained NAM/NAC-conserved domains and most of them were predicted to be located on the nucleus. Phylogenetic analysis showed that these 249 TdNACs can be classified into seven clades, which are likely to be involved in the regulation of grain protein content, starch synthesis and response to biotic and abiotic stresses. Expression pattern analysis revealed that TdNACs were highly expressed in different wheat tissues such as grain, root, leaves and shoots. We found that TdNAC8470 was phylogenetically close to NAC genes that regulate either grain protein or starch accumulation. Overexpression of TdNAC8470 in rice showed increased grain starch concentration but decreased grain Fe, Zn and Mn contents compared with wild-type plants. Protein interaction analysis indicated that TdNAC8470 might interact with granule-bound starch synthase 1 (TdGBSS1) to regulate grain starch accumulation. Our work provides a comprehensive understanding of the NAC TFs family in wild emmer wheat and establishes the way for future functional analysis and genetic improvement of increasing grain starch content in wheat.

Overexpression of TdNACB improves the drought resistance of rice.

Drought stress greatly affects disrupts the productivity, ecological structure, physiological and biochemical activities of wheat at different growth stages. However, drought stress tolerance is a complex quantitative trait and involves multiple metabolic pathways. We found that a wild emmer introgression line BAd7-209 had stronger drought resistance compared with drought resistant wheat Zhongmai 175. The transcriptome analysis found 14,284, 22,383 and 21,451 genes had expression corresponding responsed to drought stress at 24h, 48h, 120h, respectively and significantly enriched in 'Arginine and proline metabolism' and 'Peroxisome' in BAd7-209. 1666 transcription factors (TFs) related responsed to drought stress in which TdNACB showed high expression at 24h, 48h and 120h and had the closest relationship with TaNAC48 and OsNAC6 in phylogenetic analysis. Overexpression of TdNACB significantly enhanced drought resistance in rice and overexpression lines had significantly higher CAT, POD and SOD activity, Pro content and lower MDA content than those of the WT under drought stress. The result demonstrated that TdNACB positively regulates drought resistance through increasing proline content and enhancing activity of enzyme related to ROS scavenging. The results of this study provides candidate genes for improving wheat drought resistance and guide as reference for studying the molecular mechanisms of wheat drought resistance.

Comparison of the Agronomic, Cytological, Grain Protein Characteristics, as Well as Transcriptomic Profile of Two Wheat Lines Derived From Wild Emmer.

Two advanced wheat lines BAd7-209 and BAd23-1 without the functional gene GPC-B1 were obtained from a cross between common wheat cultivar Chuannong 16 (CN16) and wild emmer wheat accession D97 (D97). BAd7-209 showed superior quality parameters than those of BAd23-1 and CN16. We found that the components of glutenins and gliadins in BAd7-209 and BAd23-1 were similar, whereas BAd7-209 had higher amount of glutenins and gliadins than those of BAd23-1. RNA sequencing analysis on developing grains of BAd7-209 and BAd23-1 as well as their parents revealed 382 differentially expressed genes (DEGs) between the high-grain protein content (GPC) (D97 + BAd7-209) and the low-GPC (CN16 + BAd23-1) groups. DEGs were mainly associated with transcriptional regulation of the storage protein genes, protein processing in endoplasmic reticulum, and protein export pathways. The upregulated gluten genes and transcription factors (e.g., NAC, MYB, and bZIP) may contribute to the high GPC in BAd7-209. Our results provide insights into the potential regulation pathways underlying wheat grain protein accumulation and contribute to make use of wild emmer for wheat quality improvement.