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Widespread concerns over the impact of human activity on the environment have resulted in a desire to replace artificial functional materials with naturally derived alternatives. As such, polysaccharides are drawing increasing attention due to offering a renewable, biodegradable, and biocompatible feedstock for functional nanomaterials. In particular, nanocrystals of cellulose and chitin have emerged as versatile and sustainable building blocks for diverse applications, ranging from mechanical reinforcement to structural coloration. Much of this interest arises from the tendency of these colloidally stable nanoparticles to self-organize in water into a lyotropic cholesteric liquid crystal, which can be readily manipulated in terms of its periodicity, structure, and geometry. Importantly, this helicoidal ordering can be retained into the solid-state, offering an accessible route to complex nanostructured films, coatings, and particles. In this review, the process of forming iridescent, structurally colored films from suspensions of cellulose nanocrystals (CNCs) is summarized and the mechanisms underlying the chemical and physical phenomena at each stage in the process explored. Analogy is then drawn with chitin nanocrystals (ChNCs), allowing for key differences to be critically assessed and strategies toward structural coloration to be presented. Importantly, the progress toward translating this technology from academia to industry is summarized, with unresolved scientific and technical questions put forward as challenges to the community.
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BACKGROUND: Molecularly targeted therapies have recently become a hotspot in the treatment of LUAD, with ongoing efforts to identify new effective targets due to individual variability. Among these potential targets, the mitochondrial transcription elongation factor (TEFM) stands out as a crucial molecule involved in mitochondrial synthetic transcriptional processing. Dysregulation of TEFM has been implicated in the development of various diseases; however, its specific role in LUAD remains unclear. METHODS: We conducted a comprehensive analysis of TEFM expression in LUAD, leveraging data from the TCGA database. Subsequently, we validated these findings using clinical specimens obtained from the First Affiliated Hospital of Soochow University, employing western blotting and qRT-PCR techniques. Further experimental validation was performed through the transfection of cells with TEFM overexpression, knockdown, and knockout lentiviruses. The effects of TEFM on LUAD were evaluated both in vitro and in vivo using a range of assays, including CCK-8, colony formation, EdU incorporation, Transwell migration, Tunel assay, flow cytometry, JC-1 staining, and xenograft tumour models. RESULTS: Our investigation uncovered that TEFM exhibited elevated expression levels in LUAD and exhibited co-localization with mitochondria. Overexpression of TEFM facilitated malignant processes in LUAD cells, whereas its silencing notably curbed these behaviors and induced mitochondrial depolarization, along with ROS production, culminating in apoptosis. Moreover, the absence of TEFM substantially influenced the expression of mitochondrial transcripts and respiratory chain complexes. Results from nude mouse xenograft tumors further validated that inhibiting TEFM expression markedly hindered tumor growth. CONCLUSION: TEFM promotes LUAD malignant progression through the EMT pathway and determines apoptosis by affecting the expression of mitochondrial transcripts and respiratory chain complexes, providing a new therapeutic direction for LUAD-targeted therapy.
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Apoptosis , Mitocondrias , Factores de Elongación Transcripcional , Humanos , Animales , Mitocondrias/metabolismo , Línea Celular Tumoral , Factores de Elongación Transcripcional/metabolismo , Factores de Elongación Transcripcional/genética , Apoptosis/genética , Neoplasias Pulmonares/patología , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/terapia , Ratones Desnudos , Regulación Neoplásica de la Expresión Génica , Proliferación Celular , Carcinoma de Pulmón de Células no Pequeñas/patología , Carcinoma de Pulmón de Células no Pequeñas/genética , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Carcinoma de Pulmón de Células no Pequeñas/terapia , Movimiento Celular , Ensayos Antitumor por Modelo de Xenoinjerto , Ratones , Especies Reactivas de Oxígeno/metabolismo , Terapia Molecular Dirigida , Femenino , MasculinoRESUMEN
BACKGROUND: Diabetic nephropathy (DN) is one of the serious microvascular complications of diabetes mellitus (DM). A growing body of research has demonstrated that the inflammatory state plays a critical role in the incidence and development of DN. Pyroptosis is a new way of programmed cell death, which has the particularity of natural immune inflammation. The inhibition of inflammatory cytokine expression and regulation of pathways related to pyroptosis may be a novel strategy for DN treatment. The aim of this study is to identify pyroptosis-related genes and potential drugs for DN. METHODS: DN differentially expressed pyroptosis-related genes were identified via bioinformatic analysis Gene Expression Omnibus (GEO) dataset GSE96804. Dataset GSE30528 and GSE142025 were downloaded to verify pyroptosis-related differentially expressed genes (DEGs). Least absolute shrinkage and selection operator (LASSO) regression analysis was used to construct a pyroptosis-related gene predictive model. A consensus clustering analysis was performed to identify pyroptosis-related DN subtypes. Subsequently, Gene Set Variation Analysis (GSVA), Gene Ontology (GO) function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were conducted to explore the differences between DN clusters. A protein-protein interaction (PPI) network was used to select hub genes and DGIdb database was utilized to screen potential therapeutic drugs/compounds targeting hub genes. RESULTS: A total of 24 differentially expressed pyroptosis-related genes were identified in DN. A 16 gene predictive model was conducted via LASSO regression analysis. According to the expression level of these 16 genes, DN cases were divided into two subtypes, and the subtypes are mainly associated with inflammation, activation of immune response and cell metabolism. In addition, we identified 10 hub genes among these subtypes, and predicted 65 potential DN therapeutics that target key genes. CONCLUSION: We identified two pyroptosis-related DN clusters and 65 potential therapeutical agents/compounds for DN, which might shed a light on the treatment of DN.
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Diabetes Mellitus , Nefropatías Diabéticas , Humanos , Nefropatías Diabéticas/tratamiento farmacológico , Nefropatías Diabéticas/genética , Piroptosis/genética , Apoptosis , Análisis por Conglomerados , Biología Computacional , InflamaciónRESUMEN
BACKGROUND: Pleural solitary fibrous tumors (pSFTs) are rare mesenchymal pleural tumors with rich vascularity. Surgical resection is the cornerstone of pSFTs treatment, requiring careful preoperative imaging to delineate lesion extent and vascular supply including contrast-enhanced computed tomography and other examinations depending on its size and characteristics. CASE PRESENTATION: The patient was a 34-year-old female with a mass measuring approximately 67 × 42 × 65 mm in the left posterior mediastinum. Intraoperatively, the mass demonstrated rich vascularity. Two veins originating from the abdominal cavity entered the lower pole, one converged from the superior pole, draining into the brachiocephalic vein. Additionally, two arteries arose directly from the descending aorta, while several veins drained into the intercostal veins. In response to unexpected intraoperative vascular findings, vascular clips and silk threads were used to ligate them. Subsequently, the tumor was successfully dissected, with approximately 600 ml of blood loss recorded during the 4-hour surgery. The patient exhibited a satisfactory postoperative recovery, and follow-up spanning over six months revealed no indications of recurrence or metastasis. CONCLUSIONS: We firstly present a case of successful resection of a pSFT in a 34-year-old woman with a distinct feeding vessel arising from the descending aorta and describe the related surgical procedures. This case highlights preoperative evaluation of mass vascularity based on contrast-enhanced computed tomography. When blood supply is challenging to clarify, angiography can offer additional details, especially for giant pSFTs. Despite this, thorough intraoperative exploration remains essential to detect unexpected vessels. Appropriate interventions should be customized based on the vascular origins and the surrounding anatomical structures.
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Aorta Torácica , Humanos , Femenino , Adulto , Aorta Torácica/cirugía , Aorta Torácica/diagnóstico por imagen , Tumor Fibroso Solitario Pleural/cirugía , Tumor Fibroso Solitario Pleural/diagnóstico por imagen , Tomografía Computarizada por Rayos XRESUMEN
We built a portable microchip electrophoresis heavy metal ion detection system and proposed a pH-mediated field amplified sample stacking (pH-mediated FASS) online preconcentration method. The pH-mediated FASS focuses and stacks heavy metal cations by controlling electrophoretic mobilities with a pH change between the analyte and the background electrolyte (BGE) in solution to improve the detection sensitivity of the system. We optimized and adjusted sample matrix solution (SMS) ratios and pH to create concentration and pH gradients for SMS and BGE. Furthermore, we optimize the microchannel width to improve the preconcentration effect further. The system and method analyzed soil leachates polluted with heavy metals and separated Pb2+ and Cd2+ within 90 s, obtaining their levels at 58.01 mg/L and 4.91 mg/L with sensitivity enhancement factors (SEF) of 26.40 and 43.73. Compared with inductively coupled plasma atomic emission spectrometry (ICP-AES), the detection error of the system was less than 8.80%.
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Non-small cell lung cancer (NSCLC) is one of the prevalent and deadly cancers worldwide. Cisplatin (CDDP) has been used as a standard adjuvant therapy for advanced NSCLC patients, while chemoresistance is one of the most challenging problems to limit its clinical application. Our data showed that the expression of visfatin was significantly increased in CDDP resistant NSCLC cells as compared with that in their parental cells, while knockdown of visfatin or its neutralization antibody can restore the CDDP sensitivity of resistant NSCLC cells. The upregulation of visfatin in CDDP resistant NSCLC cells was due to the increased mRNA stability and promoter activity. Further, we found that signal transducer and activator of transcription 3 (STAT3), which was increased in chemoresistant cells, can increase the transcription of visfatin. While tristetraprolin (TTP), which can decease mRNA stability of visfatin, was decreased in chemoresistant cells. Inhibition of STAT3 or over expression of TTP can restore CDDP sensitivity of resistant NSCLC cells. Collectively, our data showed that STAT3 and TTP-regulated expression of visfatin was involved in CDDP resistance of NSCLC cells. It indicated that targeted inhibition of visfatin should be a potential approach to overcome CDDP resistance of NSCLC treatment.
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Carcinoma de Pulmón de Células no Pequeñas/fisiopatología , Citocinas/metabolismo , Resistencia a Antineoplásicos/fisiología , Neoplasias Pulmonares/fisiopatología , Nicotinamida Fosforribosiltransferasa/metabolismo , Línea Celular Tumoral , Regulación Neoplásica de la Expresión Génica/fisiología , Humanos , Estabilidad del ARN/fisiología , Factor de Transcripción STAT3/metabolismo , Tristetraprolina/metabolismo , Regulación hacia Arriba/fisiologíaRESUMEN
The structural coloration of arthropods often arises from helicoidal structures made primarily of chitin. Although it is possible to achieve analogous helicoidal architectures by exploiting the self-assembly of chitin nanocrystals (ChNCs), to date no evidence of structural coloration has been reported from such structures. Previous studies are identified to have been constrained by both the experimental inability to access sub-micrometer helicoidal pitches and the intrinsically low birefringence of crystalline chitin. To expand the range of accessible pitches, here, ChNCs are isolated from two phylogenetically distinct sources of α-chitin, namely fungi and shrimp, while to increase the birefringence, an in situ alkaline treatment is performed, increasing the intensity of the reflected color by nearly two orders of magnitude. By combining this treatment with precise control over ChNC suspension formulation, structurally colored chitin-based films are demonstrated with reflection tunable from blue to near infrared.
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Quitina , Nanopartículas , Quitina/química , Nanopartículas/química , SuspensionesRESUMEN
Antibiotic resistance is a worsening global concern, and the environmental behaviors and migration patterns of antibiotic resistance genes (ARGs) have attracted considerable interest. Understanding the long-range transport of ARG pollution is crucial. In this study, we characterized the dynamics of ARG changes after their release into aquatic environments and demonstrated the importance of traditional chemical contaminants in the transmission mechanisms of ARGs. We hypothesized that the main route of ARG proliferation switches from active transmission to passive transmission. This antibiotic-dominated switch is motivated and affected by non-corresponding contaminants. The effect of anthropogenic activities gradually weakens from inland aquatic environments to ocean environments; however, the effect of changes in environmental conditions is enhanced along this gradient. The insights discussed in this study will help to improve the understanding of the distribution and migration of ARG pollution in various aquatic environments, and provide a modern perspective to reveal the effect of corresponding contaminants and non-corresponding contaminants in the process of antibiotic resistance proliferation.
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Farmacorresistencia Microbiana/genética , Contaminantes Químicos del Agua/análisis , Antibacterianos , Ambiente , Monitoreo del Ambiente , Genes Bacterianos , Microbiología del Agua , Contaminación Química del Agua/estadística & datos numéricosRESUMEN
Understanding the antibiotic resistance transmission mechanisms and migration dynamics of antibiotic-resistant bacteria (ARB) in the natural environment is critical given the increasing prevalence of antibiotic resistance. The aim of this study was to examine the fate of sulfonamide-resistant fecal bacteria (E. coli) in an estuary ecosystem and to explore the role and contribution of environmental factors in this process. The prevalence of sulfonamide-resistance status of E. coli was analyzed over different seasons in two estuary systems. Environmental factors and disturbance indices of anthropogenic activities were evaluated by detecting antibiotic concentrations, heavy metal abundance and other physicochemical parameters. The abundances of antibiotic-resistant E. coli were significantly attenuated during land-sea migration suggesting that estuary environments play a natural mitigation role in the contamination of freshwaters by antibiotic-resistant E. coli. Additionally, environmental factors and disturbance indices of anthropogenic activities significantly correlated with the distribution and migration of antibiotic-resistant E. coli in the estuaries. Lastly, simulation experiments suggested differential adaptability between antibiotic-resistant and non-resistant E. coli towards environmental changes in estuary environments. Meanwhile, our results indicate that low concentrations of antibiotics will not increase the competitive advantage of resistant E. coli in estuaries.
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Antibacterianos/farmacología , Farmacorresistencia Bacteriana , Microbiología Ambiental , Escherichia coli/efectos de los fármacos , Estuarios , Migración Humana , Sulfonamidas/farmacología , Transmisión de Enfermedad Infecciosa , Escherichia coli/aislamiento & purificación , Infecciones por Escherichia coli/microbiología , Infecciones por Escherichia coli/transmisión , Humanos , PrevalenciaRESUMEN
The environmental behaviors and migration patterns of antibiotic resistance genes (ARGs) have attracted considerable research interest. However, there has been little research concerning the effects of corresponding and non-corresponding contaminants on the fate of ARGs in coastal environments. In the present study, the distribution of intI1, sul1, sul2, qnrS and aac(6')-Ib were analyzed in water and sediment samples of Laizhou Bay in the context of corresponding and non-corresponding contaminants. The abundance of intI1, sul1 and sul2 genes exhibited a clear decreasing trend extending from the inner estuary to the coastal area. Strong and positive correlations existed between sul1/sul2 and sulfonamide antibiotic residues in sediments, and between the abundances of intI1 and sul1/sul2. Statistical analyses indicated that non-corresponding contaminants were partially correlated with ARG abundances. These results suggest that non-corresponding contaminants may have direct or indirect influences on the abundances of ARGs and intI1 in the Laizhou Bay.
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Bahías/microbiología , Farmacorresistencia Microbiana/genética , Monitoreo del Ambiente/métodos , Genes Bacterianos , Quinolonas/análisis , Sulfonamidas/análisis , Contaminantes Químicos del Agua/análisis , Bahías/química , China , Estuarios , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiologíaRESUMEN
Although challenging, fabrication of porous conducting polymeric materials with excellent electronic properties is crucial for many applications. We developed a fast in situ polymerization approach to pure polyaniline (PANI) hydrogels, with vanadium pentoxide hydrate nanowires as both the oxidant and sacrifice template. A network comprised of ultrathin PANI nanofibers was generated during the in situ polymerization, and the large aspect ratio of these PANI nanofibers allowed the formation of hydrogels at a low solid content of 1.03 wt %. Owing to the ultrathin fibril structure, PANI hydrogels functioning as a supercapacitor electrode display a high specific capacitance of 636 F g-1, a rate capability, and good cycling stability (â¼83% capacitance retention after 10,000 cycles). This method was also extended to the preparation of polypyrrole and poly(3,4-ethylenedioxythiophene) hydrogels. This template polymerization method represents a rational strategy for design of conducing polymer networks, which can be readily integrated in high-performance devices or a further platform for functional composites.
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The fabrication of thin films comprising ordered nanowire assemblies with emerging, precisely defined properties and adjustable functionalities enables highly integrated technologies in the fields of microelectronics and micro system technology, as well as for efficient power generation, storage, and utilization. Shear force, theoretically, is deemed the most promising method for obtaining in-plane, uniaxial thin films comprising nanowires. The success depends largely on the assembly process, and uniform structural control throughout multiple length scales can be achieved only if a rational strategy is executed. Here, we report that in shearing processes dopants such as lyotropic cellulose nanorods can give rise to the uniaxial alignment of V2O5· nH2O nanowires. Our systematic study indicates that this finding, namely, the nanocombing effect, can be a general principle for the continuous production of uniaxial thin films comprising densely packed nanowires varying in chemical composition and aspect ratios. Conversion of the V2O5· nH2O constituents via in situ oxidative polymerization leads to in-plane, uniaxial polyaniline (PANI) thin films with anisotropic electric and optical properties, which are otherwise difficult to fabricate due to the poor processability of PANI. The uniaxial PANI thin films can be utilized to fabricate flexible gas sensors for distinguishing various analytes, including similar homologues such as methanol and ethanol. We expect the methodology to be applied to a broad spectrum of synthetic and biogenic nanowires for the integration of their collective properties in high-performance electronic devices.
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A key requirement for the understanding of crystal growth is to detect how new layers form and grow at the nanoscale. Multistage crystallization pathways involving liquid-like, amorphous or metastable crystalline precursors have been predicted by theoretical work and have been observed experimentally. Nevertheless, there is no clear evidence that any of these precursors can also be relevant for the growth of crystals of organic compounds. Herein, we present a new growth mode for crystals of DL-glutamic acid monohydrate that proceeds through the attachment of preformed nanoscopic species from solution, their subsequent decrease in height at the surface and final transformation into crystalline 2D nuclei that eventually build new molecular layers by further monomer incorporation. This alternative mechanism provides a direct proof for the existence of multistage pathways in the crystallization of molecular compounds and the relevance of precursor units larger than the monomeric constituents in the actual stage of growth.
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This corrects the article DOI: 10.1038/ncomms15933.
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With the exacerbating problem of antibiotic resistance, antibiotic resistance genes (ARGs) as emerging contaminants are found at elevated levels in inland aquatic environments, especially in regions of intensive agricultural and urban activity. However, little quantitative data exist on the migration and attenuation of ARGs in estuary ecosystem, which is central to predicting their fate after release into marine environment. Moreover, the relevance of multiple chemical contaminants and water quality constituents should be understood to amplify and attenuate antibiotic resistance levels. To determine the prevalence and examine the fate of sulfonamide ARGs (sul-ARGs) in two estuaries under different effects of anthropogenic activities, we analyzed the sul-ARGs (sul1, sul2, and sul3), class 1 integrons (int1), and bacterial biomass in surface water samples from Daliaohe and Liaohe river estuaries. We also evaluated five types of antibiotics, heavy metals, and various bulk water quality constituents. Results showed that sul-ARGs were widespread in Daliaohe and Liaohe river estuaries, but the distribution did not correlate with the concentration of sulfonamides. Significant reduction in the abundance of sul-ARGs was also observed with increased salinity. Nevertheless, the trend in the change of concentrations of sul-ARGs was different in the two estuaries. Statistical analysis of the results indicated that several metals were significantly and positively correlated with sul-ARGs. Pearson's correlation coefficients were higher than those determined between antibiotic residues and sul-ARGs. Furthermore, the relative abundance of sul-ARGs was significantly and positively correlated with the relative abundance of int1 which suggested that the propagation of sul-ARGs was facilitated by class 1 integrons in estuaries.
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Farmacorresistencia Microbiana/genética , Genes Bacterianos , Sulfonamidas/análisis , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente , EstuariosRESUMEN
Antibiotic resistance gene (ARG) residues and the mode of transmission in marine environments remain unclear. The sulfonamide (SAs) concentrations, different genes and total bacterial abundance in seawater and sediment of the Northern Yellow Sea were analyzed. Results showed the genes sul I and sul II were present at relatively high concentrations in all samples, whereas the gene sul III was detected fewer. The ARGs concentrations in the sediment were 10(3) times higher than those in water, which indicated sediment was essential ARG reservoir. Statistical analysis revealed the total antibiotic concentration was positively correlated with the relative abundance of the gene sul I and sul II. The relative abundances of the gene sul I and the gene sul II were also correlated positively with those of the gene int1. This correlation demonstrated that SAs exerted selective pressure on these ARGs, whereas the gene int1 could be implicated in the propagation of the genes sul I and sul II in marine environments.