RESUMEN
Ghrelin is implicated in the regulation of gastric functional development. The octanoylation of ghrelin is critical for its physiological functions which dependent upon ghrelin O-acyltransferase (GOAT) catalyzation. To investigate the effect of GOAT on gastric acid secretion and expression of ghrelin in vitro. Primary cultures of gastric mucosal cells were challenged with 1.5â¯×â¯10-5, 1.5â¯×â¯10-4 and 1.5â¯×â¯10-3â¯mol/mL GO-CoA-Tat (The GOAT inhibitor), respectively, for 24â¯h in order to further clarify the effect of GOAT on H+-K+-ATPase activity. In vitro, GO-CoA-Tat significantly increased ghrelin and GOAT mRNA expression at 1.5â¯×â¯10-5, 1.5â¯×â¯10-4 and 1.5â¯×â¯10-3â¯mol/mL, and augmented cell total ghrelin secretion at 1.5â¯×â¯10-3â¯mol/mL. But cell acylated ghrelin secretion was reduced at 1.5â¯×â¯10-3â¯mol/mL GO-CoA-Tat (Pâ¯<â¯0.05). And cell acylated ghrelin synthesis was reduced at 1.5â¯×â¯10-4 and 1.5â¯×â¯10-3â¯mol/mL GO-CoA-Tat (Pâ¯<â¯0.05). In accordance with acylated ghrelin level, H+-K+-ATPase activity were decreased with 1.5â¯×â¯10-4 and 1.5â¯×â¯10-3â¯mol/mL GO-CoA-Tat (Pâ¯<â¯0.05). These results indicated that GOAT inhibitor decreases the acylated ghrelin level and H+-K+-ATPase activity in vitro.