1.
Zhonghua Gan Zang Bing Za Zhi
; 11(8): 501-2, 2003 Aug.
Artículo
en Zh
| MEDLINE
| ID: mdl-12939190
Asunto(s)
Hepatitis C Crónica/tratamiento farmacológico , Interferón-alfa/administración & dosificación , Ribavirina/administración & dosificación , Antivirales/administración & dosificación , Quimioterapia Combinada , Hepacivirus/efectos de los fármacos , Hepacivirus/genética , Humanos , Interferón-alfa/efectos adversos , Resultado del Tratamiento
2.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 17(1): 77-80, 2003 Mar.
Artículo
en Zh
| MEDLINE
| ID: mdl-12870026
RESUMEN
BACKGROUND: To test susceptibility of human liver cell line Hep G2 to HCV in vitro. METHODS: Hep G2 was cultivated with the serum from a chronic hepatitis C patient. After inoculation, plus and minus strand of HCV RNA, the expression of HCV NS3 antigen and the location of HCV RNA in cell and/or supernatant were examined by RT-PCR, immunohistochemistry and in situ hybridization, respectively. RESULTS: HCV RNA could be detected from day 2 to day 40 post-inoculation in both cell and supernatant. HCV NS3 antigen could be expressed in infected cells and HCV RNA was mainly situated within cytoplasm. CONCLUSIONS: The results suggested that HepG2 cell line was not only susceptible to HCV but also could support its replication in vitro.