Effects of bioaugmentation by isolated Achromobacter xylosoxidans BP1 on PAHs degradation and microbial community in contaminated soil.

Polycyclic aromatic hydrocarbons (PAHs) are a group of organic pollutants ubiquitous and persistent in soil. In order to provide a viable solution for bioremediation of PAHs-contaminated soil, a strain of Achromobacter xylosoxidans BP1 with superior PAHs degradation ability was isolated from contaminated soil at a coal chemical site in northern China. The degradation of phenanthrene (PHE) and benzo[a]pyrene (BaP) by strain BP1 was investigated in three different liquid phase cultures, and the removal rates of PHE and BaP by strain BP1 were 98.47% and 29.86% after 7 days under the conditions of PHE and BaP as the only carbon source, respectively. In the medium with the coexistence of PHE and BaP, the removal rates of BP1 were 89.44% and 9.42% after 7 days, respectively. Then, strain BP1 was investigated for its feasibility in remediating PAH-contaminated soil. Among the four PAHs-contaminated soils treated differently, the treatment inoculated with BP1 exhibited higher removal rates of PHE and BaP (p < 0.05), especially the CS-BP1 treatment (inoculation of BP1 into unsterilized PAHs-contaminated soil) showed 67.72%, 13.48% removal of PHE and BaP, respectively, over 49 days of incubation. Bioaugmentation also significantly increased the activity of dehydrogenase and catalase in the soil (p＜0.05). Furthermore, the effect of bioaugmentation on the removal of PAHs was investigated by measuring the activity of dehydrogenase (DH) and catalase (CAT) during incubation. Among them, the DH and CAT activities of CS-BP1 and SCS-BP1 (inoculation of BP1 into sterilized PAHs-contaminated soil) treatments inoculated with strain BP1 were significantly higher than those of treatments without BP1 addition during incubation (p < 0.01). The structure of the microbial community varied among treatments, but the Proteobacteria phylum showed the highest relative abundance in all treatments of the bioremediation process, and most of the bacteria with higher relative abundance at the genus level also belonged to the Proteobacteria phylum. Prediction of microbial functions in soil by FAPROTAX analysis showed that bioaugmentation enhanced microbial functions associated with the degradation of PAHs. These results demonstrate the effectiveness of Achromobacter xylosoxidans BP1 as a PAH-contaminated soil degrader for the risk control of PAHs contamination.

Heavy metal levels in the soil near typical coal-fired power plants: partition source apportionment and associated health risks based on PMF and HHRA.

In this study, five priority metals recommended by the Ministry of Ecology and Environment of China (MEEC) were investigated. In the Bijie region of Guizhou Province, three typical coal-fired power plants were chosen as the research locations. A combination of 24 soil samples was obtained at various distances and depths from the point source of contamination. The authors found that the average contents of As, Cd, Cr, Ni, and Pb were 14.15, 1.52, 16.80, 40.71, and 53.00 mg kg-1, respectively, with Cd and Pb pollution prominent. In another, soil heavy metal (SHM) content tends to increase or decrease dependently with the increase of sampling distance and depth, with total concentrations ranging from 77.14 to 157.33 mg kg-1. Combining PCA and PMF models, the number of source factors was determined more clearly and accurately using PCA, and the Q-value of PMF was used for validation. The PCA-PMF indicated that the primary anthropogenic sources were transportation-related activities and emissions from coal combustion. The health risks of SHMs under three different exposure routes were then assessed using the HHRA. The findings showed the five HMs in order of non-carcinogenic risk were As > Pb > Cr > Ni > Cd. The comprehensive non-carcinogenic risk for children under the oral intake route around plant B and C was greater than 1, pointing to a potential health risk to children from the soils. The carcinogenic risk of HM was less than 1.00E-04 for both single-factor and multifactor under all three exposure routes, which is below the tolerable limit.

Combined toxic effects of nanoplastics and norfloxacin on antioxidant and immune genes in mussels.

Nanoplastics (NPs) and antibiotics (ABs) are two of the emerging marine contaminants that have drawn the most attention in recent years. Given the necessity of figuring out the effects of plastic and antibiotic contamination on marine organism life and population in the natural environment, it is essential to apply rapid and effective biological indicators to evaluate their comprehensive toxic effects. In this study, using mussel (Mytilus coruscus) as a model, we investigated the combined toxic effects of NP (80 nm polystyrene beads) and AB (Norfloxacin, NOR) at environmental-relevant concentrations on antioxidant and immune genes. In terms of the antioxidant genes, NPs significantly increased the relative expression of Cytochrome P450 3A-1 (CYP3A-1) under various concentrations of NOR conditions, but they only significantly increased the relative expression of CYP3A-2 in the high concentration (500 µg L-1 NOR) co-exposure group. In the NP-exposure group which exposed to no or low concentrations of NOR, nuclear factor erythroid 2-related factor 2 (Nrf2) was upregulated. In terms of the immune genes, interleukin-1 receptor-associated kinase (IRAK) -1 showed a significant increase in the low-concentration NOR group while a significant inhibition in the high-concentration NOR group. Due to the presence of NPs, exposure to NOR resulted in a significant increase in both IRAK-4 and heat shock protein (HSP) 70. Our findings indicate that polystyrene NPs can exacerbate the effects of NOR on the anti-oxidant and immune defense performance of mussels. This study delves into the toxic effects of NPs and ABs from a molecular perspective. Given the expected increase in environmental pollution due to NPs and ABs, future research is needed to investigate the potential synergistic effect of NPs and ABs on other organisms.

Size-dependent effects of plastic particles on antioxidant and immune responses of the thick-shelled mussel Mytilus coruscus.

Micro-/nano-plastic particles (MNPs) are present in the ocean with potential detrimental impacts on marine ecosystems. Bivalves are often used as marine bioindicators and are ideal to evaluate the threat posed by various-sized MNPs. We exposed the mussel Mytilus coruscus to MNPs with different particle sizes (70 and 500 nm, 5, 10 and 100 µm) for 3, 72 h and 30 days. The antioxidant responses in digestive gland and the hemolymph were then evaluated. The time of exposure played a strong modulating role in the biological response. A 3-hour exposure had no significant impact on the digestive gland. After 72 h, an increase in oxidative stress was observed in the digestive gland, including increased hydrogen peroxide (H2O2) level, catalase (CAT), glutathione peroxidase (GPx) activities and malondialdehyde (MDA) production. After a 30-day exposure, the oxidative stress decreased while lipid peroxidation increased. A 30-day exposure increased hemocyte mortality (HM) and reactive oxygen species (ROS) levels in the hemolymph, while phagocytosis (PA), lysosome content (LC), mitochondrial number (MN) and mitochondrial membrane potential (MMP) significantly decreased. Longer-term exposure to MNPs caused oxidative stress in the digestive gland as well as impaired viability and immunity of hemocytes. Particle size also influenced the response with smaller particles having more severe effects. A depuration for 7 days was enough to reverse the negative effects observed on the digestive gland and hemolymph. This study provides new insights on the effects of small-sized MNPs, especially nanoplastic particles (NPs), on aquatic organisms, and provides a solid theoretical knowledge background for future studies on toxic effects of MNPs.

[Toxicological Effects of Enrofloxacin and Its Removal by Freshwater Micro-Green Algae Dictyosphaerium sp.]

Enrofloxacin (ENR), a fluoroquinolones antibiotic, is widely used in the medical and aquaculture fields. Its residues in surface waters in China are high. However, few studies have evaluated both its toxicity to phytoplankton and the degradation or removal by microalgae. In this study, the growth, photosynthetic activity, and exopolysaccharides (EPS) of freshwater micro-green algae Dictyosphaerium sp. and the dynamics of ENR concentrations (0, 5, 25, 50, and 100 mg·L-1) were studied through an exposure experiment for 12 days. Results showed that the biomass and photosynthetic pigment content of Dictyosphaerium sp. increased with increasing exposure time in each treatment; however, it showed a significant inhibitory effect on the growth and pigment accumulation of Dictyosphaerium sp. compared with the control group (P<0.01). The LC50 of ENR to Dictyosphaerium sp. was (241.29±7.33) mg·L-1 after 96-h exposure, indicating that Dictyosphaerium sp. could adapt to the stress conditions of high concentration ENR. Meanwhile, when the concentration of enrofloxacin was<5 mg·L-1, it was found to promote the maximum photosynthetic rate (Fv/Fm) of Dictyosphaerium sp. On the contrary, when the concentration of enrofloxacin was>5 mg·L-1, photosynthetic inhibition was observed (P<0.01). The actual photosynthetic rate (Yield) and the maximum electron transfer rate (ETRmax) showed a trend of initially decreasing and then increasing in 12 days. It can gradually adapt to the stress conditions and recover certain photosynthetic activity after 6 days' exposure. In addition, ENR can also stimulate the EPS (RPS and CPS) release. At the end of the experiment, the removal rates of ENR in the four control groups (no algae addition groups) (5, 25, 50, and 100 mg·L-1ENR) were 7.27%, 5.56%, 5.30%, and 4.88%, respectively, while the removal rates of the treatment groups were 3.21, 3.01, 2.69, and 2.83 times of the no algae groups, indicating that Dictyosphaerium sp. had a significant promoting effect on the removal of ENR (P<0.01). Overall, our results can provide new insights for the understanding of the ecological toxicity of fluoroquinolone antibiotics to primary producers in the aquatic system and also provide new ideas for the ecological removal of antibiotic residues in water bodies and the biological resource utilization of freshwater microalgae.