Pleiotropic Role of Dietary Phytochemicals in Cancer: Emerging Perspectives for Combinational Therapy.

Cancer is considered a complicated health issue worldwide. The mean cancer survival through standard therapeutic strategies has not been significantly improved over the past few decades. Hence, alternate remedies are needed to treat or prevent this dreadful disease being explored. Currently, it has been recognized that repeated treatment with chemotherapeutic agents has been largely ineffective due to multidrug resistance and further conventional treatment possesses limited drug accessibility to cancerous tissues, which in turn necessitates a higher dose resulting in increased cytotoxicity. Drug combinations have been practiced to address the problems associated with conventional single drug treatment. Recently, natural dietary agents have attracted much attention in cancer therapy because of their synergistic effects with anticancer drugs against different types of cancer. Natural phytochemicals may execute their anticancer activity through targeting diverse cancer cell signaling pathways, promoting cell cycle arrest and apoptosis, regulating antioxidant status and detoxification. This review focuses mainly on the anticancer efficacy of dietary phytochemicals in combination with standard therapeutic drugs reported from various in vitro and in vivo experimental studies apart from clinical trials. This review adds knowledge to the field of intervention studies using combinational modalities that opens a new window for cancer treatment/chemoprevention.

Polymeric Amines and Ampholytes Derived from Poly(acryloyl chloride): Synthesis, Influence on Silicic Acid Condensation and Interaction with Nucleic Acid.

Polymeric amines are intensively studied due to various valuable properties. This study describes the synthesis of new polymeric amines and ampholytes by the reaction of poly(acryloyl chloride) with trimethylene-based polyamines containing one secondary and several (1⁻3) tertiary amine groups. The polymers contain polyamine side chains and carboxylic groups when the polyamine was in deficiency. These polymers differ in structure of side groups, but they are identical in polymerization degree and polydispersity, which facilitates the study of composition-properties relationships. The structure of the obtained polymers was confirmed with 13C nuclear magnetic resonance infrared spectroscopy, and acid-base properties were studied with potentiometry titration. Placement of the amine groups in the side chains influences their acid-base properties: protonation of the amine group exerts a larger impact on the amine in the same side chain than on the amines in the neighboring side chains. The obtained polymers are prone to aggregation in aqueous solutions tending to insolubility at definite pH values in the case of polyampholytes. Silicic acid condensation in the presence of new polymers results in soluble composite nanoparticles and composite materials which consist of ordered submicrometer particles according to dynamic light scattering and electron microscopy. Polymeric amines, ampholytes, and composite nanoparticles are capable of interacting with oligonucleotides, giving rise to complexes that hold promise for gene delivery applications.

Nano interfaced biosensor for detection of choline in triple negative breast cancer cells.

Choline, a type of Vitamin B, is an important nutrient in the human body and is involved in key metabolic pathways. Abnormal levels of choline leads to diseased conditions. The levels of choline and its associated compounds are found to be elevated in triple negative breast cancer (TNBC) patients. The choline level ranges from 0.4 to 4.9mmol/kg in TNBC. Thus the detection of choline levels in cells can aid in diagnosing breast cancer. The present work aims to develop a nano-interfaced electrochemical biosensor for the rapid detection of choline in cancer cells. For electrochemical detection, glassy carbon electrode coated with a zinc oxide nano-interface was used as the working electrode. Zinc oxide synthesized by hydrothermal method was characterized using SEM and XRD. The choline oxidase (ChOx) enzyme was immobilized on the nano-interface by drop-casting. Choline oxidase (ChOx) converts choline to betaine and H2O2 in the presence of oxygen. The H2O2 produced was determined amperometrically. The amount of H2O2 produced is directly proportional to concentration of choline present. The sensitivity, selectivity, stability and concentration studies were carried out and quantification of choline in TNBC was also carried out. The results demonstrate that this biosensor has the potential to be developed as a clinical tool for breast cancer detection.

Electrochemical enzymeless detection of superoxide employing naringin-copper decorated electrodes.

Flavonoid-metal ion complexes are a new class of molecules that have generated considerable interest due to their superior anti-oxidant and pharmacological activities. The metal ion present in these complexes can participate in redox reactions by toggling between different oxidation states. This property can be invaluable for sensing applications. But, the use of flavonoid-metal ion complexes as sensors remains an unexplored facet. The present work attempts to develop a non-enzymatic superoxide sensor using naringin-copper complex. Detection of superoxide has been mainly based on enzymes and cytochromes. However, these sensors are limited by their poor structural stability and high cost. The naringin-copper based non-enzymatic sensor exhibits good sensitivity in a range of 0.2-4.2 µM with a response time of <1 s. The performance of the sensor is not affected by pH and common interferents.