Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
1.
Environ Sci Technol ; 56(16): 11865-11877, 2022 08 16.
Artículo en Inglés | MEDLINE | ID: mdl-35929951

RESUMEN

Biocrusts covering drylands account for major fractions of terrestrial biological nitrogen fixation and release large amounts of gaseous reactive nitrogen (Nr) as nitrous acid (HONO) and nitric oxide (NO). Recent investigations suggested that aerobic and anaerobic microbial nitrogen transformations occur simultaneously upon desiccation of biocrusts, but the spatio-temporal distribution of seemingly contradictory processes remained unclear. Here, we explore small-scale gradients in chemical concentrations related to structural characteristics and organism distribution. X-ray microtomography and fluorescence microscopy revealed mixed pore size structures, where photoautotrophs and cyanobacterial polysaccharides clustered irregularly in the uppermost millimeter. Microsensor measurements showed strong gradients of pH, oxygen, and nitrite, nitrate, and ammonium ion concentrations at micrometer scales in both vertical and lateral directions. Initial oxygen saturation was mostly low (∼30%) at full water holding capacity, suggesting widely anoxic conditions, and increased rapidly upon desiccation. Nitrite concentrations (∼6 to 800 µM) and pH values (∼6.5 to 9.5) were highest around 70% WHC. During further desiccation they decreased, while emissions of HONO and NO increased, reaching maximum values around 20% WHC. Our results illustrate simultaneous, spatially separated aerobic and anaerobic nitrogen transformations, which are critical for Nr emissions, but might be impacted by future global change and land management.


Asunto(s)
Cianobacterias , Suelo , Óxido Nítrico , Nitritos , Nitrógeno/análisis , Ácido Nitroso/química , Óxido Nitroso/análisis , Suelo/química
2.
Environ Microbiol ; 18(5): 1428-39, 2016 05.
Artículo en Inglés | MEDLINE | ID: mdl-26310431

RESUMEN

Lichens are obligate symbioses between fungi and green algae or cyanobacteria. Most lichens resynthesize their symbiotic thalli from propagules, but some develop within the structures of already existing lichen symbioses. Diploschistes muscorum starts as a parasite infecting the lichen Cladonia symphycarpa and gradually develops an independent Diploschistes lichen thallus. Here we studied how this process influences lichen-associated microbiomes and photobionts by sampling four transitional stages, at sites in Sweden and Germany, and characterizing their microbial communities using high-throughput 16S rRNA gene and photobiont-specific ITS rDNA sequencing, and fluorescence in situ hybridization. A gradual microbiome shift occurred during the transition, but fractions of Cladonia-associated bacteria were retained during the process of symbiotic reorganization. Consistent changes observed across sites included a notable decrease in the relative abundance of Alphaproteobacteria with a concomitant increase in Betaproteobacteria. Armatimonadia, Spartobacteria and Acidobacteria also decreased during the infection of Cladonia by Diploschistes. The lichens differed in photobiont specificity. Cladonia symphycarpa was associated with the same algal species at all sites, but Diploschistes muscorum had a flexible strategy with different photobiont combinations at each site. This symbiotic invasion system suggests that partners can be reorganized and selected for maintaining potential roles rather than depending on particular species.


Asunto(s)
Ascomicetos/fisiología , Bacterias/aislamiento & purificación , Líquenes/microbiología , Microbiota , Simbiosis , Alphaproteobacteria/genética , Alphaproteobacteria/aislamiento & purificación , Bacterias/genética , Betaproteobacteria/genética , Betaproteobacteria/aislamiento & purificación , Hibridación Fluorescente in Situ , ARN Ribosómico 16S/genética
3.
Noise Health ; 17(79): 387-93, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26572698

RESUMEN

This study aimed to evaluate the impact of traffic noise along the motorway on sleep quality, sleepiness, and vigilant attention in long-haul truck drivers. This was a randomized, crossover, within-subject controlled study. Healthy long-haul truck drivers spent 6 consecutive nights in a real truck berth with full sleep laboratory equipment. During 3 nights, subjects were exposed to replayed traffic noise alongside motorways, whereas the other 3 nights were without traffic noise. Polysomnography was recorded during the nights and numerous sleepiness tests and vigilance examinations were performed during the following standardized working day. Outcome measures were compared between noisy and silent nights using the paired Wilcoxon test. Ten healthy long-haul truck drivers with a mean age of 36.3 ± 7.3 years completed the study as planned. On noisy nights, subjects had greater latencies to the rapid eye movement (REM) phase (90 ± 32 min vs 69 ± 16 min, P = 0.074) and higher percentages of sleep stage 1 (13.7 ± 5.5% vs 11.2 ± 4.4%; P = 0.059). Subjects also rated their sleep quality as having been better during nights without noise (28.1 ± 3.7 vs 30.3 ± 6.2, P = 0.092). The impact of these differences on daytime sleepiness and vigilance was rather low; however, mean Karolinska Sleepiness Scale (KSS) scores measured during the course of the following day were higher on six out of eight occasions after noisy nights. The effects of overnight traffic noise on sleep quality are detectable but unlikely to have any major impact on the vigilant attention and driving performance of long haul-truck drivers with low nocturnal noise sensitivity. This might not be true for subgroups prone to sleeping disorders.


Asunto(s)
Nivel de Alerta , Atención , Vehículos a Motor , Ruido en el Ambiente de Trabajo/efectos adversos , Ruido del Transporte/efectos adversos , Exposición Profesional/efectos adversos , Sueño , Adulto , Conducción de Automóvil , Estudios Cruzados , Humanos , Masculino , Persona de Mediana Edad , Proyectos Piloto , Polisomnografía
4.
Dalton Trans ; 53(24): 10220-10225, 2024 Jun 17.
Artículo en Inglés | MEDLINE | ID: mdl-38828484

RESUMEN

Bis-silylenes do not only act as strong chelating σ-donor ligands, but also exhibit cooperative behaviour in the activation of small molecules. Three different P-Si containing molecules were prepared from the reaction between tBuCP and different bis-silylenes, which are bridged by ferrocenediyl, diaminobenzene, or o-carborane.

5.
JIMD Rep ; 63(4): 371-378, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35822096

RESUMEN

Mucopolysaccharidosis type I (MPS I) is an autosomal-recessive metabolic disorder caused by an enzyme deficiency of lysosomal alpha-l-iduronidase (IDUA). Haematopoietic stem cell transplantation (HSCT) is the therapeutic option of choice in MPS I patients younger than 2.5 years, which has a positive impact on neurocognitive development. However, impaired growth remains a problem. In this monocentric study, 14 patients with MPS I (mean age 1.72 years, range 0.81-3.08) were monitored according to a standardised follow-up program after successful allogeneic HSCT. A detailed anthropometric program was carried out to identify growth patterns and to determine predictors of growth in these children. All patients are alive and in outpatient care (mean follow-up 8.1 years, range 0.1-16.0). Progressively lower standard deviation scores (SDS) were observed for body length (mean SDS -1.61; -4.58 - 3.29), weight (-0.56; -3.19 - 2.95), sitting height (-3.28; -7.37 - 0.26), leg length (-1.64; -3.88 - 1.49) and head circumference (0.91; -2.52 - 6.09). Already at the age of 24 months, significant disproportions were detected being associated with increasing deterioration in growth for age. Younger age at HSCT, lower counts for haemoglobin and platelets, lower potassium, higher donor-derived chimerism, higher counts for leukocytes and recruitment of a matched unrelated donor (MUD) positively correlated with body length (p ≤ 0.05). In conclusion, this study characterised predictors and aspects of growth patterns in children with MPS I after HSCT, underlining that early HSCT of MUD is essential for slowing body disproportion.

6.
FEMS Microbiol Ecol ; 95(3)2019 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-30668688

RESUMEN

Borderline lichens are simple mutualistic symbioses between fungi and algae, where the fungi form loose mycelia interweaving algal cells, instead of forming a lichen thallus. Schizoxylon albescens shows two nutritional modes: it can either live as a borderline lichen on Populus tremula bark or as a saprotroph on Populus wood. This enables us to investigate the microbiota diversity in simple fungal-algal associations and to study the impact of lichenization on the structure of bacterial communities. We sampled three areas in Sweden covering the distribution of Schizoxylon, and using high-throughput sequencing of the 16S rRNA gene and fluorescence in situ hybridization we characterized the associated microbiota. Bacterial communities in lichenized and saprotrophic Schizoxylon were clearly distinct, but when comparing the microbiota with the respective substrates, only the fruiting bodies show clear differences in composition and abundance from the communities in the substrates. The colonization by either lichenized or saprotrophic mycelia of Schizoxylon did not significantly influence the microbiota in the substrate. This suggests that in a morphologically simple form of lichenization, as represented by the Schizoxylon-Coccomyxa system, algal-fungal interactions do not significantly influence bacterial communities, but a more complex structure of the lichen thallus is likely required for hosting specific microbiota.


Asunto(s)
Ascomicetos/fisiología , Líquenes/microbiología , Microbiota , Populus/microbiología , Simbiosis , Ascomicetos/genética , Bacterias/clasificación , Bacterias/genética , Chlorophyta/genética , Chlorophyta/microbiología , Chlorophyta/fisiología , Cuerpos Fructíferos de los Hongos/fisiología , Líquenes/genética , Microbiota/genética , Corteza de la Planta/microbiología , ARN Ribosómico 16S/genética , Suecia , Madera/microbiología
7.
Sci Rep ; 9(1): 6468, 2019 04 23.
Artículo en Inglés | MEDLINE | ID: mdl-31015576

RESUMEN

Biological soil crusts (biocrusts) occur within drylands throughout the world, covering ~12% of the global terrestrial soil surface. Their occurrence in the deserts of the Arabian Peninsula has rarely been reported and their spatial distribution, diversity, and microbial composition remained largely unexplored. We investigated biocrusts at six different locations in the coastal and central deserts of Oman. The biocrust types were characterized, and the bacterial and fungal community compositions of biocrusts and uncrusted soils were analysed by amplicon sequencing. The results were interpreted based on the environmental parameters of the different sites. Whereas at lowland sites, mainly cyanobacteria-dominated biocrusts were observed, both cyanobacteria- and lichen-dominated biocrusts occurred at mountain sites. The majority of bacterial sequences (32-83% of total sequences) belonged to Actinobacteria, Cyanobacteria, Alphaproteobacteria, and Bacteroidetes, whereas fungal sequences belonged to Ascomycota, Basidiomycota, and Chytridiomycota (>95%). With biocrust development, a notable increase in cyanobacterial and decrease in actinobacterial proportions was observed for cyanobacteria-dominated crusts. In coastal areas, where salinity is high, biocrusts were replaced by a unique marine mat-like microbial community, dominated by halotolerant taxa. Redundancy analysis revealed a significant contribution of soil texture, cover type, carbon content, and elevation to the variations in bacterial and fungal communities. Multivariate analysis placed microbial communities in significantly separated clusters based on their carbon content, elevation and electrical conductivity. We conclude that Oman hosts a variety of cyanobacteria- and lichen-dominated crusts with their bacterial and fungal communities being largely dictated by soil properties and environmental parameters.


Asunto(s)
Bacterias , Biodiversidad , Clima Desértico , Hongos , Micobioma , Microbiología del Suelo , Suelo , Animales , Bacterias/clasificación , Bacterias/genética , Hongos/clasificación , Hongos/genética , Omán
8.
ISME J ; 12(4): 1032-1046, 2018 04.
Artículo en Inglés | MEDLINE | ID: mdl-29445133

RESUMEN

Biological soil crusts (biocrusts) cover about 12% of the Earth's land masses, thereby providing ecosystem services and affecting biogeochemical fluxes on a global scale. They comprise photoautotrophic cyanobacteria, algae, lichens and mosses, which grow together with heterotrophic microorganisms, forming a model system to study facilitative interactions and assembly principles in natural communities. Biocrusts can be classified into cyanobacteria-, lichen-, and bryophyte-dominated types, which reflect stages of ecological succession. In this study, we examined whether these categories include a shift in heterotrophic communities and whether this may be linked to altered physiological properties. We analyzed the microbial community composition by means of qPCR and high-throughput amplicon sequencing and utilized flux measurements to investigate their physiological properties. Our results revealed that once 16S and 18S rRNA gene copy numbers increase, fungi become more predominant and alpha diversity increases with progressing succession. Bacterial communities differed significantly between biocrust types with a shift from more generalized to specialized organisms along succession. CO2 gas exchange measurements revealed large respiration rates of late successional crusts being significantly higher than those of initial biocrusts, and different successional stages showed distinct NO and HONO emission patterns. Thus, our study suggests that the photoautotrophic organisms facilitate specific microbial communities, which themselves strongly influence the overall physiological properties of biocrusts and hence local to global nutrient cycles.


Asunto(s)
Microbiología del Suelo , Procesos Autotróficos , Bacterias/genética , Bacterias/aislamiento & purificación , Biodiversidad , Briófitas , Cianobacterias/genética , Cianobacterias/aislamiento & purificación , Ecosistema , Hongos/genética , Hongos/aislamiento & purificación , Procesos Heterotróficos , Líquenes/aislamiento & purificación , Suelo/química
9.
Environ Microbiol Rep ; 10(3): 264-271, 2018 06.
Artículo en Inglés | MEDLINE | ID: mdl-29488349

RESUMEN

Desert varnishes are dark rock coatings observed in arid environments and might resemble Mn-rich coatings found on Martian rocks. Their formation mechanism is not fully understood and the possible microbial involvement is under debate. In this study, we applied DNA metagenomic Shotgun sequencing of varnish and surrounding soil to evaluate the composition of the microbial community and its potential metabolic function. We found that the α diversity was lower in varnish compared to soil samples (p value < 0.05), suggesting distinct populations with significantly higher abundance of Actinobacteria, Proteobacteria and Cyanobacteria within the varnish. Additionally, we observed increased levels of transition metal metabolic processes in varnish compared to soil samples. Nevertheless, potentially relevant enzymes for varnish formation were detected at low to insignificant levels in both niches, indicating no current direct microbial involvement in Mn oxidation. This finding is supported by quantitative genomic analysis, elemental analysis, fluorescence imaging and scanning transmission X-ray microscopy. We thus conclude that the distinct microbial communities detected in desert varnish originate from settled Aeolian microbes, which colonized this nutrient-enriched niche, and discuss possible indirect contributions of microorganisms to the formation of desert varnish.


Asunto(s)
Actinobacteria/clasificación , Arcilla/microbiología , Cianobacterias/clasificación , Compuestos Férricos/metabolismo , Compuestos de Manganeso/metabolismo , Óxidos/metabolismo , Proteobacteria/clasificación , Microbiología del Suelo , Actinobacteria/genética , Actinobacteria/aislamiento & purificación , Actinobacteria/metabolismo , Cianobacterias/genética , Cianobacterias/aislamiento & purificación , Cianobacterias/metabolismo , Metagenómica/métodos , Microbiota/genética , Proteobacteria/genética , Proteobacteria/aislamiento & purificación , Proteobacteria/metabolismo , Análisis de Secuencia de ADN/métodos
10.
FEMS Microbiol Ecol ; 92(10)2016 10.
Artículo en Inglés | MEDLINE | ID: mdl-27411981

RESUMEN

Cyanobacteria associated with biological soil crusts (BSCs) have important attributes, such as nitrogen fixation and soil stabilisation. However, research on these organisms has been minimal, and their diversity and distribution throughout temperate Europe is currently unknown. The SCIN (Soil Crust International) project is a multidisciplinary research initiative that aims to achieve improved understanding of the BSCs of Europe, one facet being an investigation into the cyanobacterial communities of BSCs across a latitudinal gradient. Cyanobacteria assemblages were analysed by both morphological and molecular analysis. Two treatments were applied prior to DNA extraction, continued sample wetting and a dry sample process, and 16S ribosomal RNA (rRNA) amplicons were processed by Illumina MiSeq sequencing. The results reveal high and variable cyanobacterial diversity with each site showing a unique assemblage. Many common cyanobacterial genera, for example Nostoc and Microcoleus, were found in all sites but the abundances of different genera varied considerably. The polyphasic approach was found to be essential in recording the presence of important cyanobacteria that a single method itself did not highlight. The wet and dry treatments showed some differences in diversity, but mainly in abundance, this may suggest how cyanobacterial composition of BSCs changes with seasonal variability.


Asunto(s)
Cianobacterias/clasificación , Variación Genética , Microbiología del Suelo , Cianobacterias/genética , Ecosistema , Europa (Continente) , Fijación del Nitrógeno , ARN Ribosómico 16S/genética , Suelo
11.
Sci Total Environ ; 573: 594-602, 2016 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-27585428

RESUMEN

Aquatic ecosystems are generally affected by multiple stressors, and therefore, analysing single stressor responses is not appropriate to evaluate the whole range of effects on these ecosystems. We assessed the interaction effects of two strong stressors (higher flow velocity due to e.g. daily hydropeaking) and nutrient enrichment for an oligotrophic stream periphyton community. As periphyton has a rapid reproduction rate and very short life cycles, it can therefore be expected to reflect short-term impacts and sudden changes/disturbances in the environment. We measured biomass development, algal group distribution and photosynthesis efficiency during a time period of 33days in an experimental flume setting in Lunz am See (Austria). We conducted the experiment with two treatments (no hydropeaking and hydropeaking) and three nutrient enrichments (nitrate, phosphate and nitrate+phosphate enrichment) and control (no nutrient addition). The results showed a significant lower biomass development in the hydropeaking treatment (HP), compared to the no-hydropeaking treatment (NHP) in a later successional stage (day 33). Nutrient subsidy effects were not observed, because the biomass development (chlorophyll-a) of periphyton was highly diminished through the pulsed flow velocity increase. Also a negative synergistic interaction (more negative than predicted additively) was observed. Our study confirmed for periphyton communities that for different algal groups and functional guilds the same multiple stressor combination can be detrimental for one species group (e.g. chlorophyta) while beneficial for another (e.g. diatoms). We conclude for multiple stressor studies to consider the successional stage and community composition, when estimating the interaction effects of these stressors.


Asunto(s)
Modelos Teóricos , Nitratos/análisis , Perifiton , Fosfatos/análisis , Ríos/química , Movimientos del Agua , Biomasa , Chlorophyta/crecimiento & desarrollo , Diatomeas/crecimiento & desarrollo , Fotosíntesis , Ríos/microbiología , Especificidad de la Especie
12.
Biodivers Conserv ; 23: 1639-1658, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24954978

RESUMEN

Here we report details of the European research initiative "Soil Crust International" (SCIN) focusing on the biodiversity of biological soil crusts (BSC, composed of bacteria, algae, lichens, and bryophytes) and on functional aspects in their specific environment. Known as the so-called "colored soil lichen community" (Bunte Erdflechtengesellschaft), these BSCs occur all over Europe, extending into subtropical and arid regions. Our goal is to study the uniqueness of these BSCs on the regional scale and investigate how this community can cope with large macroclimatic differences. One of the major aims of this project is to develop biodiversity conservation and sustainable management strategies for European BSCs. To achieve this, we established a latitudinal transect from the Great Alvar of Öland, Sweden in the north over Gössenheim, Central Germany and Hochtor in the Hohe Tauern National Park, Austria down to the badlands of Tabernas, Spain in the south. The transect stretches over 20° latitude and 2,300 m in altitude, including natural (Hochtor, Tabernas) and semi-natural sites that require maintenance such as by grazing activities (Öland, Gössenheim). At all four sites BSC coverage exceeded 30 % of the referring landscape, with the alpine site (Hochtor) reaching the highest cyanobacterial cover and the two semi-natural sites (Öland, Gössenheim) the highest bryophyte cover. Although BSCs of the four European sites share a common set of bacteria, algae (including cyanobacteria) lichens and bryophytes, first results indicate not only climate specific additions of species, but also genetic/phenotypic uniqueness of species between the four sites. While macroclimatic conditions are rather different, microclimatic conditions and partly soil properties seem fairly homogeneous between the four sites, with the exception of water availability. Continuous activity monitoring of photosystem II revealed the BSCs of the Spanish site as the least active in terms of photosynthetic active periods.

13.
Front Microbiol ; 2: 275, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-22294982

RESUMEN

Sphagnum mosses represent a main vegetation component in ombrotrophic wetlands. They harbor a specific and diverse microbial community with essential functions for the host. To understand the extend of host specificity and impact of environment, Sphagnum fallax and Sphagnum angustifolium, two phylogenetically closely related species, which show distinct habitat preference with respect to the nutrient level, were analyzed by a multifaceted approach. Microbial fingerprints obtained by PCR-single-strand conformation polymorphism of 16S rRNA and nitrogenase-encoding (nifH) genes were highly similar for both Sphagnum species. Similarity was confirmed for colonization patterns obtained by fluorescence in situ hybridization (FISH) coupled with confocal laser scanning microscopy (CLSM): Alphaproteobacteria were the main colonizers inside the hyaline cells of Sphagnum leaves. A deeper survey of Alphaproteobacteria by 16S rRNA gene amplicon sequencing reveals a high diversity with Acidocella, Acidisphaera, Rhodopila, and Phenylobacterium as major genera for both mosses. Nitrogen fixation is an important function of Sphagnum-associated bacteria, which is fulfilled by microbial communities of Sphagna in a similar way. NifH libraries of Sphagnum-associated microbial communities were characterized by high diversity and abundance of Alphaproteobacteria but contained also diverse amplicons of other taxa, e.g., Cyanobacteria and Deltaproteobacteria. Statistically significant differences between the microbial communities of both Sphagnum species could not be discovered in any of the experimental approach. Our results show that the same close relationship, which exists between the physical, morphological, and chemical characteristics of Sphagnum mosses and the ecology and function of bog ecosystems, also connects moss plantlets with their associated bacterial communities.

SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA