Does Mandibular Distraction Osteogenesis for Robin Sequence Create Altered Craniofacial Morphology and Disrupt Tooth Development?

BACKGROUND: Robin Sequence (RS) infant patients may require mandibular distraction osteogenesis (MDO) to improve airway. The distracted mandible may grow vertically and the developing dentition may be disrupted. PURPOSE: The study purpose was to measure the association of MDO on craniofacial morphology and tooth development in RS subjects. STUDY DESIGN, SETTING, SAMPLE: This was a retrospective cohort study of RS infants treated with or without MDO. Inclusion criteria were RS diagnosis, complete imaging, and treatment at our pediatric regional hospital. Exclusion criteria were treatment elsewhere and insufficient imaging. EXPOSURE VARIABLE: Exposure was airway management; subjects were grouped by use of MDO or not. Subjects were compared to age-matched normal infants presurgically and to age-matched normal controls at follow-up. MAIN OUTCOME VARIABLES: Main outcome variables were craniofacial morphology measured using cephalometric gonial angle and ramus height to mandibular body length ratio presurgically (T1), postsurgically (T2), and at the mixed dentition (T3). Disrupted tooth development was assessed by absence/abnormality of teeth on radiographs at T3. COVARIATES: Covariates were age, sex, body mass index, comorbidities, and cephalometric measurements. ANALYSES: Appropriate univariate, bivariate, and regression models were computed, and significance level was set at P < .05. RESULTS: The sample contained 14 RS-MDO subjects with median age of 1.1 months and 10 (71.4%) were female. Presurgery, RS-MDO subjects had significantly more obtuse gonial angles (145° vs 137°, P = .04) and shorter mandibular bodies (32 vs 41 mm, P < .01) than the 37 unaffected controls. Increased ramus height (P < .01) and mandibular body length (P < .01) and forward rotation of the mandible were seen in 12 subjects with post-MDO imaging compared to their presurgical condition. At mixed dentition, 12 post-MDO subjects had more obtuse gonial angles (P < .01) and steeper mandibular planes (P < .01) than 19 non-MDO RS subjects. Both RS groups had different cephalometric values and more vertical measures than matched cephalometric norms. Thirty-one percent of 12 RS-MDO subjects had ≥1 teeth with abnormal development compared to none of 19 RS subjects without MDO (P = .02). CONCLUSION AND RELEVANCE: MDO increased mandibular size in infants but can disrupt the developing dentition. Postdistraction growth may result in more vertical mandibular morphology with large gonial angles.

Food insecurity and its relationship to mental health and oral health in older adults.

BACKGROUND AND OBJECTIVES: Poor oral health disproportionately affects low-income older adults, for whom food insecurity and poor mental health may affect dental health. We explored the associations between food insecurity, mental health, and dental health. Furthermore, we examined whether mental health impacted the associations between food insecurity and dental health. MATERIALS AND METHODS: We conducted a cross-sectional study with a convenience sample of 226 older adults (aged 50+), employing survey and dental screening data. Participants were recruited from seven community-based organisations in Washington State, USA. We calculated descriptive statistics and conducted Chi-square tests, t tests, and logistic regression analyses to assess the associations between aspects of dental health (untreated decay, gum disease, and unmet dental needs), mental health (depression and cognitive function), and food insecurity. RESULTS: In our sample, food insecurity was observed in 28.4%, 40.6% had untreated decay, 31.6% gum disease, and 42.5% unmet dental needs. Food insecurity was associated with a higher occurrence of untreated decay and unmet dental needs. Participants experiencing food insecurity had higher odds of gum disease (aOR = 2.3; 95% CI: 1.1, 5.2) and unmet dental needs (aOR = 3.2; 95% CI:1.4, 7.6). Greater gum disease due to food insecurity was observed among individuals with lower levels of cognitive impairment. CONCLUSION: Food insecurity is associated with poorer oral health among older adults and cognitive function may modify this relationship. These findings underscore the importance of addressing both food insecurity and cognitive impairment as integral components of efforts to improve the oral health of older adults.

Association between crowding estimation and extraction recommendations in orthodontics.

INTRODUCTION: Little is known about how precisely orthodontists in the United States (US) assess crowding or at what range of crowding they recommend extraction. This study aimed to assess the relationship between estimated crowding in patients with a Class I relationship and extraction recommendation by orthodontists in the US. The secondary aims were to evaluate the accuracy and precision of clinician estimations and determine if clinician background traits play a role in extraction decision-making. METHODS: An electronic survey was prepared using 4 patients with a Class I relationship with anterior crowding selected from a University Orthodontics Clinic and was sent to approximately 10,400 subjects through Facebook and the American Association of Orthodontists Partners in Research program. RESULTS: From the 297 responses received, most clinicians recommended extraction once crowding reached 9-10 mm in either the maxilla or the mandible. The data from 2 patients suggest this decision was more strongly correlated with mandibular crowding. Clinician estimations varied widely but, on average, were precise within approximately 2 mm of objective measurements. There was a tendency to overestimate crowding, especially by Northeastern practitioners. Clinicians who reported routinely measuring crowding or who reported that they recommended extractions to >10% of their patients were 1.2-2.0 and 1.4-1.6 times more likely, respectively, to recommend extraction in the patients. CONCLUSIONS: Crowding estimation was highly subjective and varied widely among clinicians. Most clinicians recommended extraction once maxillary or mandibular crowding approximated 9-10 mm. Some clinician demographics were correlated with the precision and accuracy of estimations and the likelihood of extraction in the patients.

Occlusal contact changes in patients treated with clear aligners: A retrospective evaluation using digital dental models.

INTRODUCTION: This study used digital intraoral scans to evaluate how clear aligner treatment affects occlusal contacts and to determine the influence of sex and age on contact changes. Results were compared with contact changes that occur during fixed appliance therapy. METHODS: Patients included in this study were treated in a University setting and private practice. Inclusion criteria were a Class I malocclusion treated nonextraction with clear aligners and the presence of pretreatment and posttreatment digital intraoral scans. Scans were imported into specialized software, and occlusal contacts were analyzed. The effects of age and sex on contact changes during clear aligner treatment were determined. Changes in occlusal contacts were compared with changes that occur during nonextraction treatment of patients with a Class I relationship using fixed edgewise appliances. RESULTS: A total of 45 clear aligner patients fit the eligibility criteria. Clear aligner treatment reduced the percentage of tight, near, and approximating contacts, whereas the percentage of open and no contacts increased. These changes in occlusal contacts were greater for the older age group studied. Genderinfluenced occlusal contact changes in the anterior dentition only where the decrease in near contacts and increase in open contacts were greater for males. These results for patients treated with clear aligners were similar to those for patients treated with fixed appliances; both treatment modalities reduced close occlusal contacts at the time active treatment was completed. CONCLUSIONS: These results indicated that when clear aligners or fixed appliances are used to treat a Class I malocclusion, the resulting occlusion immediately after debonding is not as "tight" as it was at pretreatment.

Factors Contributing to Canine Impaction in Patients With Unilateral Cleft Lip and Palate Undergoing Alveolar Bone Grafts.

BACKGROUND: Patients with unilateral cleft lip and palate (UCLP) undergo alveolar bone grafting (ABG) to unite the bony segments before eruption of the maxillary permanent canine. PURPOSE: This study assessed the frequency of canine impaction after ABG in the UCLP patient population and identified associated demographic, anatomical, and operative factors. STUDY DESIGN, SETTING, SAMPLE: This retrospective cohort study included 257 patients with UCLP who received ABG surgery at a single craniofacial center. PREDICTOR VARIABLES: The variables were cleft characteristics, pre-ABG procedures, operative factors, and dental anomalies identified through electronic health records, radiographs, and intraoral photographs taken before ABG and at least 2 years after ABG. MAIN OUTCOME VARIABLE: The primary outcome variable was the presence of an impacted maxillary canine, defined as malposition of a fully developed canine following ABG with full eruption of the contralateral canine. ANALYSES: The t tests, χ2 tests, and logistic regression analysis were used to evaluate frequency of canine impaction and test for associations with the predictor variables. RESULTS: Of 257 patients, 56% were male with a mean age of 9.2 years at the time of ABG. The frequency of canine impaction was 27% (n = 69, 95% confidence interval 22 to 33%). Significant associations were found for: complete clefts (30 vs 12%, P = .017), hypodivergent facial patterns (low angle: 46%; high angle: 29 vs 22% normal angle, P = .042), females (adjusted odds ratio [aOR] = 2.1, P = .015), early grafting with less than 1/3 developed canine root (aOR = 3.36, P = .024), pregraft extraction of primary canine (aOR = 2.3, P = .009) and greater permanent canine angulation (odds ratio = 1.1, P < .0001). No significant associations with pregraft orthodontic expansion or regrafting were detected. CONCLUSION AND RELEVANCE: In this study, about one in four patients with UCLP had an impacted canine. Multiple factors including pregraft extraction of the primary canine and increased canine angulation were associated with impaction of the permanent canine.

Survey and electronic health record-based medication use agreement in children with cystic fibrosis: A retrospective cross-sectional study.

BACKGROUND: Medication use is important to collect accurately in medically complex patients in both clinical and research settings. AIM: We assessed patient-level agreement for medication use between self-reported survey and electronic health record (EHR) for children with cystic fibrosis (CF). METHODS: Our retrospective cross-sectional study focused on children with CF ages 6-20 years from Seattle Children's Hospital in Washington state, USA (N = 85). A self- or parent-reported survey included questions on current use of specific medications and antibiotic use in the past 2 months. We compared survey data with data abstracted from the individual's EHR and derived Cohen's Kappa statistics to estimate the level of agreement between the two methods. RESULTS: Self-reported medication use was generally higher in the survey than in the EHR. The level of agreement ranged from slight for probiotics (74.1% agreement; 95% confidence interval [CI]: 64.6%-83.6%; kappa: 0.07), pancreatic enzymes (80% agreement; 95% CI: 71.3%-88.7%; kappa: 0.12), and vitamin D (55.3% agreement; 95% CI: 44.5%-66.1%; kappa: 0.20) to moderate for chronic azithromycin (80% agreement; 95% CI: 7.13%-88.7%; kappa: 0.50), proton pump inhibitors (76.5% agreement; 95% CI: 67.3%-85.7%; kappa: 0.46), and oral antibiotics (70.6% agreement; 95% CI: 60.7%-80.5%; kappa: 0.42). CONCLUSION: There is considerable heterogeneity in level of agreement in medication use between self-reported survey and EHR data for children with CF. Standardized approaches are needed to improve the accuracy of medication data collected in clinical practice and research.

Prescription drug monitoring program use and utility by Washington State pharmacists: A mixed-methods study.

OBJECTIVES: To explore factors and situations that influence pharmacists to use the prescription drug monitoring program (PDMP) and to characterize actions taken by pharmacists after alarming scenarios from a PDMP query. DESIGN: Explanatory sequential 2-phase mixed-methods design: (1) cross-sectional Web-based survey of Washington State pharmacists followed by (2) interviews with purposefully selected respondents to explore statistically significant quantitative findings. SETTING AND PARTICIPANTS: The study was conducted in Washington State from September 2018 to February 2019. A total of 967 Washington State pharmacists from various practice settings, including inpatient and outpatient pharmacies, participated. Ten outpatient pharmacists were interviewed in the second phase. OUTCOME MEASURES: The pharmacists reported the frequency of PDMP use, opinion on the usefulness of PDMP, and action(s) taken after a concerning PDMP report. RESULTS: The usable response rate for pharmacists with a PDMP account was 17.6% (818/4659), and usable response rate for all pharmacists was 10.4% (967/9263). PDMP use varied by race, practice setting, and employer policy on PDMP use. Among the 818 PDMP users, 396 (48%) used the database at least once during a shift. Frequent PDMP users were more likely to recommend naloxone compared with less frequent users (adjusted odds ratio 1.70 [95% CI 1.09-2.65], P = 0.02). The following 3 interview themes were identified: time, company policy, and red flags. CONCLUSION: PDMP has value to pharmacists of all practice settings studied. Frequent PDMP use may facilitate more pharmacist interventions, such as a naloxone prescription.

Dental enamel defects predict adolescent health indicators: A cohort study among the Tsimane' of Bolivia.

OBJECTIVES: Bioarchaeological findings have linked defective enamel formation in preadulthood with adult mortality. We investigated how defective enamel formation in infancy and childhood is associated with risk factors for adult morbidity and mortality in adolescents. METHODS: This cohort study of 349 Amerindian adolescents (10-17 years of age) related extent of enamel defects on the central maxillary incisors (none, less than 1/3, 1/3 to 2/3, more than 2/3) to adolescent anthropometrics (height, weight) and biomarkers (hemoglobin, glycated hemoglobin, white blood cell count, and blood pressure). Risk differences and 95% confidence intervals were estimated using multiple linear regression. Enamel defects and stunted growth were compared in their ability to predict adolescent health indicators using log-binomial regression and receiver operating characteristics (ROCs). RESULTS: Greater extent of defective enamel formation on the tooth surface was associated with shorter height (-1.35 cm, 95% CI: -2.17, -0.53), lower weight (-0.98 kg, 95% CI: -1.70, -0.26), lower hemoglobin (-0.36 g/dL, 95% CI: -0.59, -0.13), lower glycated hemoglobin (-0.04 %A1c , 95% CI: -0.08, -0.00008), and higher white blood cell count (0.74 109 /L, 95% CI: 0.35, 1.14) in adolescence. Extent of enamel defects and stunted growth independently performed similarly as risk factors for adverse adolescent outcomes, including anemia, prediabetes/type II diabetes, elevated WBC count, prehypertension/hypertension, and metabolic health. CONCLUSIONS: Defective enamel formation in infancy and childhood predicted adolescent health outcomes and may be primarily associated with infection. Extent of enamel defects and stunted growth may be equally predictive of adverse adolescent health outcomes.

A novel non-opioid protocol for medically supervised opioid withdrawal and transition to antagonist treatment.

BACKGROUND: The clinical feasibility of a novel non-opioid and benzodiazepine-free protocol was assessed for the treatment of medically supervised opioid withdrawal and transition to subsequent relapse prevention strategies. METHODS: A retrospective chart review of DSM-IV diagnosed opioid-dependent patients admitted for inpatient medically supervised withdrawal examined 84 subjects (52 males, 32 females) treated with a 4-day protocol of scheduled tizanidine, hydroxyzine, and gabapentin. Subjects also received ancillary medications as needed, and routine counseling. Primary outcomes were completion of medically supervised withdrawal, and initiation of injectable extended release (ER) naltrexone treatment. Secondary outcomes included the length of hospital stay, Clinical Opiate Withdrawal Scale (COWS) scores, and facilitation to substance use disorder treatment intervention. Ancillary medication use and adverse effects were also assessed. RESULTS: A total of 79 (94%) of subjects completed medically supervised withdrawal. A total of 27 (32%) subjects chose to pursue transition to ER naltrexone, and 24 of the 27 (89%) successfully received the injection prior to hospital discharge. The protocol subjects had a mean length of hospital stay of 3.6 days, and the mean COWS scores was 3.3, 3.4, 2.8, and 2.4 on Day 1, 2, 3, and 4, respectively. Furthermore, 71 (85%) engaged in an inpatient or outpatient substance use disorder (SUD) treatment program following protocol completion. CONCLUSION: This retrospective chart review suggests the feasibility of a novel protocol for medically supervised opioid withdrawal and transition to relapse prevention strategies, including injectable ER naltrexone. This withdrawal protocol does not utilize opioid agonists or other controlled substances.‬‬‬‬.

Malnutrition-related early childhood exposures and enamel defects in the permanent dentition: A longitudinal study from the Bolivian Amazon.

OBJECTIVES: We investigated the relationship between early childhood malnutrition-related measures and subsequent enamel defects in the permanent dentition. MATERIALS AND METHODS: This cohort study included 349 Amerindian adolescents (10-17 years, 52% male) from the Bolivian Amazon. Exposures included: stunted growth (height-for-age z-scores), underweight (weight-for-age z-scores), anemia (hemoglobin), acute inflammation (C-reactive protein) and parasitic infection (hookworm). We measured the occurrence (no/yes) and extent (<1/3, 1/3-2/3, >2/3) of enamel defects. We estimated associations between childhood exposures and enamel defect measures using log-binomial and multinomial logistic regression. RESULTS: The prevalence of an enamel defect characterized by an orange peel texture on a large central depression on the labial surface of the central maxillary incisors was 92.3%. During childhood (1-4 years), participants had a high prevalence of stunted growth (75.2%), anemia (56.9%), acute inflammation (39.1%), and hookworm infection (49.6%). We observed associations between childhood height-for-age (OR = 0.65; P = 0.028 for >2/3 extent vs. no EH) and gastrointestinal hookworm infection (OR = 3.43; P = 0.035 for >2/3 extent vs. no defects or <1/3 extent) with enamel defects. DISCUSSION: The study describes a possibly novel form of enamel hypoplasia and provides evidence for associations of malnutrition-related measures in early childhood, including stunted growth and parasitic helminth infection, with the observed enamel defects.

Unstimulated Saliva-Related Caries Risk Factors in Individuals with Cystic Fibrosis: A Cross-Sectional Analysis of Unstimulated Salivary Flow, pH, and Buffering Capacity.

Salivary flow rate, pH, and buffering capacity are associated with dental caries, but studies from the cystic fibrosis (CF) literature are inconclusive regarding these salivary factors and caries. The aim of this study was to evaluate these factors and their associations with dental caries in individuals with CF. Unstimulated whole saliva was collected from individuals aged 6-20 years at Seattle Children's Hospital CF Clinic, USA (n = 83). Salivary flow rate was measured in milliliters per minute. Salivary pH was assessed using a laboratory pH meter. Buffering capacity was assessed by titration with HCl. The outcome measure was caries prevalence, defined as the number of decayed, missing, or filled primary and permanent tooth surfaces. Spearman's rank correlation coefficient and the t test were used to test for bivariate associations. Multiple variable linear regression models were used to (1) run confounder-adjusted analyses and (2) assess for potential interactions. There was no significant association between salivary flow rate or buffering capacity and caries prevalence. There was a significant negative association between salivary pH and caries prevalence, but this association was no longer significant after adjusting for age. There was no significant interaction between salivary flow rate and buffering capacity or between antibiotic use and the 3 salivary factors. Our results indicate that unstimulated salivary factors are not associated with dental caries prevalence in individuals with CF. Future studies should investigate other potential saliva-related caries risk factors in individuals with CF such as cariogenic bacteria levels, salivary host defense peptide levels, and medication use.

Prospective cohort clinical study assessing the 5-year survival and success of anterior maxillary zirconia-based crowns with customized zirconia copings.

STATEMENT OF PROBLEM: Studies evaluating anterior zirconia-based crowns are limited. PURPOSE: The purpose of this prospective cohort clinical study was to assess the efficacy of zirconia-based anterior maxillary crowns with 0.3-mm customized copings at the cervical third and anatomical design elsewhere for up to 5 years of service. MATERIAL AND METHODS: Eighteen participants who required an anterior maxillary crown (n=20) and who had signed a consent form approved by the University of Washington Health Sciences Center Human Subjects Division were enrolled. All preparations were standardized and prepared with an occlusal reduction of 1.5 to 2 mm and an axial reduction of 1 to 1.5 mm with 10 degrees of convergence angle. All finish lines were located on the sound tooth structure. Zirconia copings (Lava; 3M ESPE) were custom designed and milled to a 0.3-mm thickness at the cervical third and with selective thickness elsewhere to support the veneering porcelain. All restorations were luted with self-etching self-adhesive composite resin cement. Recall appointments were at 2 weeks, 6 months, and 12 months, and annually thereafter for 5 years. Modified Ryge criteria were used to assess the clinical fracture measurements, esthetics, marginal discoloration, marginal adaptation, radiographic proximal recurrent caries, and periapical pathoses. Descriptive statistics and 95% confidence intervals were used to describe the number and rate of complications and self-reported satisfaction with the crowns. RESULTS: Twenty crowns with a mean follow-up of 58.7 months were evaluated. All crowns were rated as Alfa for fracture measurements (smooth surface, no fracture/chipping). Twelve crowns were rated esthetically as Romeo (no mismatch in color and shade) and 8 as Sierra (mismatch in color and shade within normal range). Twelve crowns were rated as Alfa (no visible evidence of crevice) and 8 as Bravo (visible evidence of crevice, no penetration of explorer) for marginal integrity. Nineteen were rated as Alfa (no discoloration) and 1 as Bravo (superficial discoloration) for marginal discoloration. No proximal caries or periapical pathoses were detected in 5 years. Participants were highly satisfied with their crowns after 5 years (mean ±SD: 9.8 ±0.4 on 0 to 10 scale). CONCLUSIONS: Zirconia-based anterior maxillary crowns with customized copings with 0.3-mm thickness at the cervical third and zirconia margins performed well after 5 years of service.

Influence of aging on flexural strength of translucent zirconia for monolithic restorations.

STATEMENT OF PROBLEM: Concern has been raised with regard to the low-temperature degradation (LTD) of translucent yttria-stabilized tetragonal zirconia polycrystalline (Y-TZP) for monolithic zirconia restorations. PURPOSE: The purpose of this in vitro study was to assess the LTD behavior of 4 commercially available translucent Y-TZP materials by accelerated aging specimens in steam at 134°C, 0.2 MPa. MATERIAL AND METHODS: Thin bars (22×3×0.2 mm) of Y-TZP, including Katana ML (Kuraray Noritake Dental Inc), Katana HT13 (Kuraray Noritake Dental Inc), Prettau (Zirkonzahn), and BruxZir (Glidewell Laboratories) (n=30 for each group), were machined from sintered blocks. Control specimens were assessed in the nonaged condition. Artificially ageing (n=5 per group at 5, 50, 100, 150, and 200 hours) was conducted in steam at 134°C at 0.2 MPa. The specimens were characterized, tested in 4-point flexure, and the fracture surfaces were analyzed. The monoclinic-to-tetragonal (m/t) peak intensity ratio measured by x-ray diffraction was used to calculate the monoclinic phase fraction and monitor LTD. Linear regression with heteroscedasticity-consistent robust standard errors was used to test for the effect of LTD (aging time) on (σf) and m/t. The Spearman rank correlation coefficient was used to assess the relationship between σf and monoclinic phase fraction (α=.05). RESULTS: Artificial aging resulted in LTD as shown by an increase in the monoclinic phase fraction for all specimens. After aging for 200 hours, the mean ±SD monoclinic phase fraction increased from 2.90 ±0.34% to 76.1 ±0.64% for Prettau, 2.69 ±0.18% to 76.0 ±0.26% for BruxZir, 4.6 ±0.19% to 35.8 ±0.80% for Katana HT13, and 3.57 ±0.35% to 33.2 ±1.1% for Katana ML (all P<.001). Flexural strength changed from a mean ±SD of 1612 ±197 MPa to all fractured during aging for Prettau (P<.001); 1248 ±73.5 MPa to all fractured during aging for BruxZir (P<.001); 1052 ±84.2 to 1099 ±70 MPa ±130 for Katana HT13 (P=.45); and from 875 ±130 to 909 ±70 MPa (P=.82) for Katana ML. The mean flexural strength values of Prettau and BruxZir decreased with an increase in the monoclinic phase with Spearman rank correlation coefficients of -0.80 (P=.001) for Prettau and -0.63 (P=.022) for BruxZir. No significant changes in flexural strength were measured for Katana ML or Katana HT13 (P>.05). CONCLUSIONS: The LTD of Y-TZP resulted in a significant decrease in flexural strength of Prettau and BruxZir, whereas Katana ML and Katana HT13 exhibited less LTD and no significant decrease in flexural strength.

A comparative evaluation of the translucency of zirconias and lithium disilicate for monolithic restorations.

STATEMENT OF PROBLEM: Studies comparing the translucency of zirconias and lithium disilicates are limited. PURPOSE: The purpose of this in vitro study was to measure the translucency of recently developed translucent zirconias and compare them with lithium disilicate. MATERIAL AND METHODS: Five types of zirconia, Prettau Anterior (Zirkonzahn GmbH), BruxZir (Glidewell Laboratories), Katana HT, Katana ST, and Katana UT (Kurary Noritake Dental Inc), and 1 type of lithium disilicate, e.max CAD LT (Ivoclar Vivadent AG), were assessed. Non-colored zirconia test specimens (n=5) were prepared as rectangles with dimensions of 15×10×0.5 and 15×10×1.0 mm. The shade of lithium disilicate was B1. A spectrophotometer (Evolution 300 UV-Vis) with an integrating sphere was used to evaluate the total transmittance of light as a percentage (Tt%) at a wavelength of 555 nm for comparison among groups. The Welch robust test for equality of means was used to compare group means (α=.025) and post hoc pairwise comparisons among groups were performed with the Dunnett T3 method. RESULTS: For the 0.5 mm thickness groups, the Tt% was 31.90 ±0.49 for Prettau Anterior, 28.82 ±0.22 for BruxZir, 28.49 ±0.14 for Katana HT, 31.67 ±0.24 for Katana ST, 33.73 ±0.13 for Katana UT, and 40.32 ±0.25 for e-max CAD LT. Post hoc tests indicated that all groups were significantly different from each other, except for between BruxZir and Katana HT, and between Prettau Anterior and Katana ST. Katana UT was significantly more translucent than all other zirconias, and e-max CAD LT was significantly more translucent than all zirconias. For the 1.0 mm thickness groups, the Tt% was 22.58 ±0.41 for Prettau Anterior, 20.13 ±0.22 for BruxZir, 20.18 ±0.39 for Katana HT, 21.86 ±0.39 for Katana ST, 23.37 ±0.27 for Katana UT, and 27.05 ±0.56 for e-max CAD LT. Post hoc tests indicated that all materials were significantly different from each other, except for between BruxZir and Katana HT, and among Prettau Anterior, Katana ST and Katana UT which were significantly more translucent than all other zirconias and less translucent than e-max CAD LT. CONCLUSION: At a thickness of 0.5 mm, Katana UT was significantly more translucent than all other zirconias, and e-max CAD LT was significantly more translucent than all zirconias. At a thickness of 1.0 mm, Prettau Anterior, Katana ST, and Katana UT were significantly more translucent than all other zirconias and less than e-max CAD LT.

Clinical efficacy of polyvinyl siloxane impression materials using the one-step two-viscosity impression technique.

STATEMENT OF PROBLEM: Impression making is a challenging clinical procedure for both patients and dentists. PURPOSE: The purpose of this clinical study was to compare a recently introduced fast-setting polyvinyl siloxane (PVS) impression material with heavy body/light body (HB/LB) combination (Imprint 4; 3M ESPE) (experimental group) with a conventional PVS impression material with HB/LB combination (Imprint 3; 3M ESPE) (control group), using the 1-step 2-viscosity impression technique. MATERIAL AND METHODS: Two definitive impressions (1 of each material combination) were made of 20 crown preparations from 20 participants. The quality of impressions was rated by 3 evaluators (clinical evaluator, clinical operator, and dental technician) and by the patients for the level of comfort and taste of the impression materials. The order in which the 2 impressions were made with each material combination was randomized for each crown preparation. A paired t test for paired means and McNemar test for paired proportions were used for statistical comparisons (α=.05). RESULTS: Participants rated the comfort of the impression making with the experimental group significantly higher than that with the control group (P=.001). No significant differences were found in participants' rating for the taste of the impression materials (P=.46). The viscosity for tray material was rated as significantly better for the control group by the clinical operator (P=.004). The readability of the impression and visibility around the finish line were rated as significantly better for the experimental group than for the control group (P<.001). Except for the ease of removal of the stone (RS), the ratings for the 2 groups by the dental technician were similar. The ease of RS was rated as significantly better for the experimental group (P<.001). Eleven dies from the control and 9 from the experimental group were selected for fabrication of the definitive crowns (P=.65). CONCLUSION: Within the limitations of this clinical study, no significant differences were found in the overall clinical performance of the experimental and the control groups. Impressions made with both materials were clinically acceptable. Participants rated the comfort provided by the experimental group significantly better than that of the control group.

In vitro evaluation of accuracy and precision of automated robotic tooth preparation system for porcelain laminate veneers.

STATEMENT OF PROBLEM: Controlling tooth reduction for porcelain laminate veneers (PLVs) in fractions of millimeters is challenging. PURPOSE: The purpose of this study was to assess an automated robotic tooth preparation system for PLVs for accuracy and precision compared with conventional freehand tooth preparation. MATERIAL AND METHODS: Twenty maxillary central incisor tooth models were divided into 2 groups. Ten were assigned to a veneer preparation with a robotic arm according to preoperative preparation design-specific guidelines (experimental group). Ten were assigned to conventional tooth preparation by a clinician (control group). Initially, all tooth models were scanned with a 3- dimensional (3D) laser scanner, and a tooth preparation for PLVs was designed on a 3D image. Each tooth model was attached to a typodont. For the experimental group, an electric high-speed handpiece with a 0.9-mm-diameter round diamond rotary cutting instrument was mounted on the robotic arm. The teeth were prepared automatically according to the designed image. For the control group, several diamond rotary cutting instruments were used to prepare the tooth models according to preoperative preparation design guidelines. All prepared tooth models were scanned. The preoperative preparation design image and scanned postoperative preparation images were superimposed. The dimensional difference between those 2 images was measured on the facial aspect, finish line, and incisal edge. Differences between the experimental and the control groups from the 3D design image were computed. Accuracy and precision were compared for all sites and separately for each tooth surface (facial, finish line, incisal). Statistical analyses were conducted with a permutation test for accuracy and with a modified robust Brown-Forsythe Levene-type test for precision (α=.05). RESULTS: For accuracy for all sites, the mean absolute deviation was 0.112 mm in the control group and 0.133 mm in the experimental group. No significant difference was found between the 2 (P=.15). For precision of all sites, the standard deviation was 0.141 mm in the control group and 0.185 mm in the experimental group. The standard deviation in the control group was significantly lower (P=.030). In terms of accuracy for the finish line, the control group was significantly less accurate (P=.038). For precision, the standard deviation in the control group was significantly higher at the finish line (P=.034). CONCLUSIONS: For the data from all sites, the experimental procedure was able to prepare the tooth model as accurately as the control, and the control procedure was able to prepare the tooth model with better precision. The experimental group showed better accuracy and precision at the finish line.

Association between added sugar intake and dental caries in Yup'ik children using a novel hair biomarker.

BACKGROUND: Dental caries (tooth decay) is a significant public health problem in Alaska Native children. Dietary added sugars are considered one of the main risk factors. In this cross-sectional pilot study, we used a validated hair-based biomarker to measure added sugar intake in Alaska Native Yup'ik children ages 6-17 years (N = 51). We hypothesized that added sugar intake would be positively associated with tooth decay. METHODS: A 66-item parent survey was administered, a hair sample was collected from each child, and a dental exam was conducted. Added sugar intake (grams/day) was measured from hair samples using a linear combination of carbon and nitrogen ratios. We used linear and log-linear regression models with robust standard errors to test our hypothesis that children with higher added sugar intake would have a higher proportion of carious tooth surfaces. RESULTS: The mean proportion of carious tooth surfaces was 30.8 % (standard deviation: 23.2 %). Hair biomarker-based added sugar intake was associated with absolute (6.4 %; 95 % CI: 1.2 %, 11.6 %; P = .02) and relative increases in the proportion of carious tooth surfaces (24.2 %; 95 % CI: 10.6 %, 39.4 %; P < .01). There were no associations between self-reported measures of sugar-sweetened food and beverage intake and tooth decay. CONCLUSIONS: Added sugar intake as assessed by hair biomarker was significantly and positively associated with tooth decay in our sample of Yup'ik children. Self-reported dietary measures were not associated tooth decay. Most added sugars were from sugar-sweetened fruit drinks consumed at home. Future dietary interventions aimed at improving the oral health of Alaska Native children should consider use of objective biomarkers to assess and measure changes in home-based added sugar intake, particularly sugar-sweetened fruit drinks.

Proteomic analysis of saliva from patients with oral chronic graft-versus-host disease.

Chronic graft-versus-host disease (cGVHD) is an immune-mediated disorder and is the major long-term complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). The oral mucosa, including the salivary glands, is affected in the majority of patients with cGVHD; however, at present there is only a limited understanding of disease pathobiology. In this study, we performed a quantitative proteomic analysis of saliva pooled from patients with and without oral cGVHD-cGVHD(+) and cGVHD(-), respectively-using isobaric tags for relative and absolute quantification labeling, followed by tandem mass spectrometry. Among 249 salivary proteins identified by tandem mass spectrometry, 82 exhibited altered expression in the oral cGVHD(+) group compared with the cGVHD(-) group. Many of the identified proteins function in innate or acquired immunity, or are associated with tissue maintenance functions, such as proteolysis or the cytoskeleton. Using ELISA immunoassays, we further confirmed that 2 of these proteins, IL-1 receptor antagonist and cystatin B, showed decreased expression in patients with active oral cGVHD (P < .003). Receiver operating curve characteristic analysis revealed that these 2 markers were able to distinguish oral cGVHD with a sensitivity of 85% and specificity of 60%, and showed slightly better discrimination in newly diagnosed patients evaluated within 12 months of allo-HSCT (sensitivity, 92%; specificity 73%). In addition to identifying novel potential salivary cGVHD biomarkers, our study demonstrates that there is coordinated regulation of protein families involved in inflammation, antimicrobial defense, and tissue protection in oral cGVHD that also may reflect changes in salivary gland function and damage to the oral mucosa.

Socioeconomic status, food security, and dental caries in US children: mediation analyses of data from the National Health and Nutrition Examination Survey, 2007-2008.

OBJECTIVES: We examined associations of household socioeconomic status (SES) and food security with children's oral health outcomes. METHODS: We analyzed 2007 and 2008 US National Health and Nutrition Examination Survey data for children aged 5 to 17 years (n = 2206) to examine the relationship between food security and untreated dental caries and to assess whether food security mediates the SES-caries relationship. RESULTS: About 20.1% of children had untreated caries. Most households had full food security (62%); 13% had marginal, 17% had low, and 8% had very low food security. Higher SES was associated with significantly lower caries prevalence (prevalence ratio [PR] = 0.77; 95% confidence interval = 0.63, 0.94; P = .01). Children from households with low or very low food security had significantly higher caries prevalence (PR = 2.00 and PR = 1.70, respectively) than did children living in fully food-secure households. Caries prevalence did not differ among children from fully and marginally food-secure households (P = .17). Food insecurity did not appear to mediate the SES-caries relationship. CONCLUSIONS: Interventions and policies to ensure food security may help address the US pediatric caries epidemic.

Effect of hydrothermal degradation on three types of zirconias for dental application.

STATEMENT OF PROBLEM: Concern has been expressed with regard to hydrothermal aging of yttria-stabilized tetragonal zirconia polycrystalline. PURPOSE: The purpose of this study was to assess the accelerated aging characteristics of a new yttria-stabilized tetragonal zirconia polycrystalline material and 2 commercially available yttria-stabilized tetragonal zirconia polycrystalline materials by exposing specimens to hydrothermal treatments in steam at 134°C, 0.2 MPa, and at 180°C, 1.0 MPa. MATERIAL AND METHODS: Thin bars of zirconia: Prettau, Zirprime, and a new zirconia, ZirTough, n = 55 for each brand (22 × 3 × 0.2 mm) were cut and ground from blocks sintered according to the manufacturer's specifications. The control specimens for each group were evaluated in the nonaged condition, and their chemical composition was measured with energy dispersive spectroscopy. The experimental specimens were artificially aged under standard autoclave sterilization conditions, 134°C at 0.2 MPa (n = 5 per group at 5, 50, 100, 150, and 200 hours), and under standard industrial ceramic aging conditions, 180°C at 1.0 MPa (n = 5 per group at 8, 16, 24, and 48 hours). The tetragonal to monoclinic transformation was measured by using x-ray diffraction for all groups. Flexural strength was measured with a 4-point bend test (ASTM 1161-B) for all the groups, and the fracture surfaces were examined with scanning electron microscopy. The data were analyzed as a function of aging time. To test for an aging effect on the flexural strength and the monoclinic-tetragonal ratio, a 1-way ANOVA (with heteroscedasticity-consistent standard errors) was used to test for a general time effect. For the analyses of the monoclinic-tetragonal ratio, the same specimens were used at 0 hours and after aging, and the data were analyzed with an ANOVA for an incomplete block design. The relationship between flexural strength and monoclinic-tetragonal ratio was assessed with the Spearman rank correlation coefficient based on the average value at each aging. RESULTS: After 200 hours at 134°C and 0.2 MPa, the flexural strength decreased from a mean (standard deviation) of 1328 ± 89.9 MPa to all fractured during aging for Prettau (P < .001); 1041 ± 130 to 779 ± 137 MPa for Zirprime (P = .<.001) and 1436 ± 136 to 1243 ± 101 MPa for ZirTough (P = .017). After 200 hours at 134°C and 0.2 MPa, a portion of the tetragonal crystals transformed to the monoclinic phase in all specimens. The mean (standard deviation) monoclinic phase fraction increased from 3.08% ± 0.28% to 78.8% ± 2.0% for Prettau, 1.95% ± 0.48% to 74.8% ± 0.52% for Zirprime, and 12.4% ± 0.60% to 31.4% ± 4.4% for ZirTough (all P < .001). After 16 hours at 180°C and 1.0 MPa, all Prettau specimens had spontaneously fractured during aging. The Zirprime and ZirTough specimens were intact after 48 hours at 180°C and 1.0 MPa, and the mean (standard deviation) flexural strength had decreased from 1041 ± 130 MPa to 595 ± 88.4 MPa for Zirprime and 1436 ± 136 MPa to 1068 ± 76.8 MPa for ZirTough (all P < .001). The mean (standard deviation) monoclinic phase fraction increased from 3.08% ± 0.28% to 79.0% ± 0.13% for Prettau, from 1.95% ± 0.48% to 68.1% ± 4.4%, for Zirprime, and from 12.4% ± 0.60% to 39.5% ± 5.56% for ZirTough (all P<.001). The flexural strength decreased with an increase in the monoclinic phase for all the groups (Spearman rank correlation coefficients, -0.71 to -1.0). Scanning electron microscope micrographs revealed a transformed layer on the fracture surfaces. The decrease in flexural strength was related to the increase in monoclinic phase from long-term degradation. CONCLUSION: Hydrothermal aging of zirconia caused a statistically significant decrease in flexural strength of thin bars of zirconia, which was the result of the transformation from tetragonal to monoclinic crystal structure. ZirTough exhibited the least decrease in strength and smallest amount of monoclinic phase after aging.