Cytomegalovirus in Adenoma and Carcinoma Lesions: Detecting Mono-Infection and Co-Infection in Salivary Glands.

Salivary glands' neoplasms are hard to diagnose and present a complex etiology. However, several viruses have been detected in these neoplasms, such as HCMV, which can play a role in certain cancers through oncomodulation. The co-infections between HCMV with betaherpesviruses (HHV-6 and HHV-7) and polyomaviruses (JCV and BKV) has been investigated. The aim of the current study is to describe the frequency of HCMV and co-infections in patients presenting neoplastic and non-neoplastic lesions, including in the salivary gland. Multiplex quantitative polymerase chain reaction was used for betaherpesvirus and polyomavirus quantification purposes after DNA extraction. In total, 50.7% of the 67 analyzed samples were mucocele, 40.3% were adenoma pleomorphic, and 8.9% were mucoepidermoid carcinoma. Overall, 20.9% of samples presented triple-infections with HCMV/HHV-6/HHV-7, whereas 9.0% were co-infections with HCMV/HHV-6 and HCMV/HHV-7. The largest number of co-infections was detected in pleomorphic adenoma cases. All samples tested negative for polyomaviruses, such as BKV and JCV. It was possible to conclude that HCMV can be abundant in salivary gland lesions. A high viral load can be useful to help better understand the etiological role played by viruses in these lesions. A lack of JCV and BKV in the samples analyzed herein does not rule out the involvement of these viruses in one or more salivary gland lesion subtypes.

Cytokines profile in patients with acute and chronic hepatitis B infection.

Hepatitis B virus (HBV) is one of the leading causes of acute and chronic hepatitis and represents a serious public health threat. Cytokines are important chemical mediators that regulate the differentiation, proliferation, and function of immune cells, with accumulating evidence indicating that the inadequate immune responses are responsible for the elimination or persistence of HBV. This study aimed to determine the cytokine profiles (IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, and IL-17A) during HBV infection and investigate their association with genotypes. A total of 66 plasma samples, 19 from patients with acute and 47 with chronic hepatitis B infection, were subjected to biochemical tests, nested-PCR, and real-time PCR, with cytokines evaluated using a commercial BD Cytometric Bead Array Human Th1/Th2/Th17 Cytokine Kit. Healthy controls (10 individuals) were selected from blood donors with no history of liver diseases. No correlation was found between genotypes, viral load, and cytokines analyzed. All cytokines showed higher levels of production among infected individuals when compared with the control group. A positive correlation classified as moderate to strong was found between cytokines IFN-γ, TNF, IL-10, IL-6, IL-4, and IL-2 through the Spearman correlation coefficient. TNF (P = 0.009), IL-10 (P < 0.001), and IL-6 (P < 0.001) levels were higher in acute individuals compared with chronic and control groups. Theses cytokines could be involved in the elimination of virus and protection against chronicity.

Cytokine profile during occult hepatitis B virus infection in chronic hepatitis C patients.

BACKGROUND: The hepatitis B virus (HBV) is one of the leading causes of acute, chronic and occult hepatitis (OBI) representing a serious public health threat. Cytokines are known to be important chemical mediators that regulate the differentiation, proliferation and function of immune cells. Accumulating evidence indicate that the inadequate immune responses are responsible for HBV persistency. The aim of this study were to investigate the cytokines IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10 and IL-17A in patients with OBI and verify if there is an association between the levels of these cytokines with the determination of clinical courses during HBV occult infection. METHODS: 114 patients with chronic hepatitis C were investigated through serological and molecular tests, the OBI coinfected patients were subjected to the test for cytokines using the commercial human CBA kit. As controls, ten healthy donors with no history of liver disease and 10 chronic HBV monoinfected patients of similar age to OBI patients were selected. RESULTS: Among 114 HCV patients investigated, 11 individuals had occult hepatitis B. The levels of cytokines were heterogeneous between the groups, most of the cytokines showed higher levels of production detection among OBI/HCV individuals when compared to control group and HBV monoinfected pacients. We found a high level of IL-17A in the HBV monoinfected group, high levels of TNF-α, IL-10, IL-6, IL-4 and IL-2 in OBI/HCV patients. CONCLUSION: These cytokines could be involved in the persistence of HBV DNA in hepatocytes triggers a constant immune response, inducing continuous liver inflammation, which can accelerate liver damage and favor the development of liver cirrhosis in other chronic liver diseases.

Evaluation of cytopathological screening results and risk factors of women who underwent Papanicolaou test in a maternity school in Fortaleza, Ceará, Brazil.

INTRODUCTION: Papanicolaou test (Pap smear) is the standard screening test of pre-neoplastic lesions and cervical cancer. This study aimed to investigate cervical cancer screening results and risk factors such as age, reason for the examination, the epithelia detected in the sample, microbiota and signs of sexually transmitted infection (STIs) of women in a maternity school in Fortaleza, Ceará, Brazil. METHODS: In this cross-sectional study, data were retrieved of 353 women who underwent Pap smear between April 2016 and January 2017 at the Federal University of Ceará. RESULTS: Of all Pap smear samples retrieved, 54.1% (191/353) had glandular epithelium and 40.2% (142/353) had metaplastic epithelium. After statistical analyses adjusted for the final model, age ≥51 years (odds ratio = 3.47) and signs of STIs (odds ratio = 4.95) remained as risk factors. CONCLUSIONS: The diagnosis of high-grade lesions and carcinomas in patients older than 50 years indicates a deficiency in cervical screening. Women with signs and symptoms of STIs and candidiasis sought medical services more frequently than asymptomatic women, and presence of these signs and symptoms contributes to the diagnosis of cervical cancer. We highlight the importance of obtaining a correct smear sampling to allow prompt detection of all preneoplastic lesions; moreover, the implementation of human papillomavirus vaccination and an efficient routine Pap screening are necessary in low-resource settings.

Liver and Inflammatory Biomarkers Are Related to High Mortality in Hospitalized Patients with COVID-19 in Brazilian Amazon Region.

COVID-19 is a multisystem disease with many clinical manifestations, including liver damage and inflammation. The objective of this study is to analyze inflammation biomarkers in relation to the clinical outcome and respiratory symptoms of COVID-19. This is a retrospective cohort of patients with COVID-19 admitted to the Hospital Regional do Baixo Amazonas from 2020 to 2022. Data were collected from electronic medical records from admission to the 30th day of hospitalization and soon after hospital discharge. A total of 397 patients were included in the study. In the longitudinal follow-up of liver markers, a significant difference was found for AST on day 14, with a higher median in the death group. Among the hematological markers, lymphopenia was observed throughout the follow-up, with the death group having the most altered values. When comparing the evolution of biomarkers in the Non-Invasive Ventilation (NIV) and Invasive Mechanical Ventilation (IMV) groups, AST showed a significant difference only on day 14 and GGT on day 1, being greater in the IMV group, and indirect bilirubin on day 7 being more altered in the NIV group. In conclusion, death during hospitalization or a more severe form of COVID-19 was related to significant changes in liver and inflammatory biomarkers.

Risk factors related to preventable infant mortality in Espirito Santo, Brazil.

Objective: To analyse the factors associated with preventable of infant mortality, in Espirito Santo, Brazil. Methods: Data were collected from records of notifiable of infant death of the Mortality Information System. A total of 5,089 infant deaths were classified as preventable and non-preventable according to the International Collaborative Effort on Infant Mortality (ICE) and the State System of Data Analysis Foundation (SEADE) methods. To investigate the factors associated with preventable of deaths, it was applied the logistic regression. Results: Approximately, 73% of the deaths were preventable according to the ICE, while 76% were preventable according to the SEADE method. Using to both methods, it was observed that preterm birth, postneonatal death and birth weight between 3000 and above 4,000 g represented higher chances for preventable infant deaths. Furthermore, the medical care was more likely to preventable infant death only for ICE method. Conclusions: The factors related to the quality of care offered in the prenatal, prepartum and childbirth periods were more relevant for the occurrence of preventable infant death. Accordingly, it is recommended to strengthen mother-child care to detect risk pregnancies during prenatal care, as well as a hierarchical, regionalized and integrated perinatal network.

Diversity of Anal HPV and Non-HPV Sexually Transmitted Infections and Concordance with Genital Infections in HIV-Infected and HIV-Uninfected Women in the Tapajós Region, Amazon, Brazil.

The aim of this study was to classify the diversity of anal HPV and non-HPV sexually transmitted infections (STIs) and compare the concordance between anal and genital infections in HIV-infected and uninfected women living in the Tapajós region, Amazon, Brazil. A cross-sectional study was performed with 112 HIV-uninfected and 41 HIV-infected nonindigenous women. Anal and cervical scrapings were collected and analyzed for HPV, Chlamydia trachomatis (CT), Neisseria gonorrheae (NG), Trichomonas vaginalis (TV), Mycoplasma genitalium (MG), and Human alphaherpesvirus 2 (HSV-2). The Kappa test evaluated the concordance between anal and genital infections. The overall prevalence of anal HPV infection was 31.3% in HIV-uninfected and 97.6% in HIV-infected women. The most frequent anal high-risk HPV (hrHPV) types were HPV18 and HPV16 in HIV-uninfected women and HPV51, HPV59, HPV31, and HPV58 in HIV-infected women. Anal HPV75 Betapapillomavirus was also identified. Anal non-HPV STIs were identified in 13.0% of all participants. The concordance analysis was fair for CT, MG, and HSV-2, almost perfect agreement for NG, moderate for HPV, and variable for the most frequent anal hrHPV types. Thus, a high prevalence of anal HPV infection with moderate and fair concordance between anal and genital HPV and non-HPV STIs was observed in our study.

Associated factors of neonatal near miss among newborns of adolescent mothers in Brazil.

OBJECTIVE: To identify the associated factors of neonatal near miss among newborns of Brazilian adolescents and to compare their occurrence in young women aged 12 to 16 years and 17 to 19 years. METHOD: Cross-sectional, hospital-based study, using data from the study Nascer no Brasil ("Birth in Brazil") on puerperal adolescents and their newborns in all regions of Brazil. Multiple and univariate logistic regression were employed to identify the associated factors of neonatal near miss. RESULTS: The following factors were found to be associated to neonatal near miss among newborns of adolescent mothers: public source of payment (OR = 4.57, 95% CI = 2.02-10.32), having to seek help in different maternity hospitals (OR = 1.52; 95% CI = 1.05-2.20), and maternal near miss (OR = 5.92; 95% CI = 1.94-18.05), in addition to a record of low weight in a previous pregnancy (OR = 3.12; 95% CI = 1.61-6.04) and twin pregnancy (OR = 7.49; 95% CI = 3.28-16.82). CONCLUSION: Neonatal near miss affected newborns of adolescent mothers in both age groups equally. Also, the determinant factors of neonatal near miss can be mostly reduced with qualified prenatal, labor, and birth care.

Recurrent preterm birth: data from the study "Birth in Brazil".

OBJECTIVE: Describe and estimate the rate of recurrent preterm birth in Brazil according to the type of delivery, weighted by associated factors. METHODS: We obtained data from the national hospital-based study "Birth in Brazil", conducted in 2011 and 2012, from interviews with 23,894 women. Initially, we used the chi-square test to verify the differences between newborns according to previous prematurity and type of recurrent prematurity. Sequentially, we applied the propensity score method to balance the groups according to the following covariates: maternal age, socio-economic status, smoking during pregnancy, parity, previous cesarean section, previous stillbirth or neonatal death, chronic hypertension and chronic diabetes. Finally, we performed multiple logistic regression to estimate the recorrence. RESULTS: We analyzed 6,701 newborns. The rate of recurrence was 42.0%, considering all women with previous prematurity. Among the recurrent premature births, 62.2% were spontaneous and 37.8% were provider-initiated. After weighting by propensity score, we found that women with prematurity have 3.89 times the chance of having spontaneous recurrent preterm birth (ORaj = 3.89; 95%CI 3.01-5.03) and 3.47 times the chance of having provider-initiated recurrent preterm birth (ORaj = 3.47; 95%CI 2.59-4.66), compared to women who had full-term newborns. CONCLUSIONS: Previous prematurity showed to be a strong predictor for its recurrence. Thus, expanding and improving the monitoring and management of pregnant women who had occurrence of prematurity strongly influence the reduction of rates and, consequently, the reduction of infant morbidity and mortality risks in the country.

Recurrence of teenage pregnancy: associated maternal and neonatal factor outcomes. / Reincidência de gravidez na adolescência: fatores associados e desfechos maternos e neonatais.

The present study aims to analyze the socioeconomic and demographic factors associated with the recurrence of teenage pregnancy, as well as to verify the association with unfavorable maternal and neonatal outcomes. It is a cross-sectional study, based on data from "Nascer no Brasil", comprised of adolescent mothers and their newborns. Univariate and multiple logistic regression were used to indicate the factors associated with this recurrence. Recurrence of teenage pregnancy was associated with a maternal age of 17-19 years (OR=3.35; 95%CI=2.45-4.59); an inadequate education for their age (OR=4.34; 95%CI=3.50-5.39), with no intention of becoming pregnant; residency in the state capital; and the fact that the partner is the head of the family. However, as independent primiparous teenagers, there is a greater chance of hypertension and restricted intrauterine growth. It can therefore be concluded that there is a high percentage of teenage pregnancies in Brazil. Teenagers with a partner, inadequate education, and no reproductive planning are more likely to have two or more pregnancies before the age of 20, demonstrating difficulties in postponing the first pregnancy. However, as primiparous teenagers, they are more likely to have complications than multiparous teenagers.

O objetivo é analisar os fatores socioeconômicos e demográficos associados à reincidência de gravidez na adolescência, assim como verificar a associação com desfechos maternos e neonatais desfavoráveis. É um estudo transversal, a partir dos dados do "Nascer no Brasil", composto por puérperas adolescentes e seus recém-nascidos. Utilizou-se regressão logística univariada e múltipla para indicar os fatores associados à essa reincidência. A reincidência da gravidez na adolescência esteve associada à idade materna de 17-19 anos (OR=3,35; IC95%=2,45-4,59), à escolaridade inadequada para a idade (OR=4,34; IC95%=3,50-5,39), à intenção de engravidar, à residência na capital do estado e ao fato do companheiro ser chefe da família. No entanto, as adolescentes primíparas apresentaram maior chance de doença hipertensiva e crescimento intrauterino restrito. Conclui-se que há um elevado percentual de reincidência de gravidez na adolescência no Brasil. Adolescentes com companheiro, pouca escolaridade e sem planejamento reprodutivo são as mais expostas a ter duas ou mais gestações antes dos 20 anos, demostrando dificuldades em postergar a primeira gestação. Todavia as adolescentes primíparas apresentam maior chance de intercorrências do que as multíparas.

Association of Pre-S/S and Polymerase Mutations with Acute and Chronic Hepatitis B Virus Infections in Patients from Rio de Janeiro, Brazil.

Several hepatitis B virus (HBV)-related factors, including the viral load, genotype, and genomic mutations, have been linked to the development of liver diseases. Therefore, in this study we aimed to investigate the influence of HBV genetic variability during acute and chronic infection phases. A real-time nested PCR was used to detect HBV DNA in all samples (acute, n = 22; chronic, n = 49). All samples were sequenced for phylogenetic and mutation analyses. Genotype A, sub-genotype A1, was the most common genotype in the study population. A total of 190 mutations were found in the pre-S/S gene area and the acute profile revealed a greater number of nucleotide mutations (p < 0.05). However, both profiles contained nucleotide mutations linked to immune escape and an increased risk of hepatocellular carcinomas (acute, A7T; chronic, A7Q). Furthermore, 17 amino acid substitutions were identified in the viral polymerase region, including the drug resistance mutations lamivudine and entecavir (rtL180M), with statistically significant differences between the mutant and wild type strains. Owing to the natural occurrence of these mutations, it is important to screen for resistance mutations before beginning therapy.

A population-based study of the relationship between advanced maternal age and premature/early-term birth in Brazil.

OBJECTIVE: To verify whether advanced maternal age (AMA), defined as women of ≥35 years, is associated with premature and early-term birth in Brazil, according to the onset of labor (spontaneous or provider-initiated). METHODS: Cross-sectional population-based study. The "Birth in Brazil" study interviewed 23 894 puerperal women between 2011 and 2012, in all regions of Brazil. The current analysis included 17 994 adult mothers and their newborns (15 448 aged between 20-34 years, and 2536 ≥ 35 years). A propensity score was used to assess the likelihood of AMA women giving birth to premature or early-term infants (spontaneous or provider-initiated) compared to women aged 20-34 years. To balance the groups, we used maternal, prenatal, and childbirth characteristics. RESULTS: The general prematurity rate was 10.24%, of which the majority of births were spontaneous (55.73%). Conversely, early-term births were more often provider-initiated (54.81%). AMA did not increase the chance of premature births, whether spontaneous or provider-initiated. However, AMA was associated with a higher rate of provider-initiated early-term birth (OR = 1.48; 95% CI: 1.23-1.77). CONCLUSION: AMA alone does not contribute to premature birth; AMA's independent association with provider-initiated early-term birth may not be based solely on clinical indications.

Prelabor cesarean section: the role of advanced maternal age and associated factors.

OBJECTIVE: to evaluate whether advanced maternal age (AMA) is associated with prelabor cesarean section and to identify the factors associated with prelabor cesarean section in AMA women, according to the mode of type of labor financing (private or public). METHODS: Based on the Birth in Brazil survey, the research was conducted on representative sample of mothers for the country (Brazil), regions, type of hospital and location (capital or not), in 2011/2012. This study included 15,071 women from two age groups: 20-29 years and ≥ 35 years. The information was collected from interviews with puerperal woman, prenatal cards, and medical records of mothers and newborns. Multiple logistic regression modelling was used to verify the association between prelabor cesarean section and maternal, prenatal and childbirth characteristics, according to the mode of financing. RESULTS: Our results showed a higher use of prelabor cesarean section for AMA (≥ 35 years) women in the public service (OR = 1.63; 95%CI 1.38-1.94) and in the private service (OR = 1.44; 95%CI 1.13-1.83), compared with women aged 20-29 years. In the adjusted model, we recorded three factors associated with the prelabor cesarean section in AMA women in both, public and private sectors: the same professional in prenatal care and childbirth (OR = 4.97 and OR = 4.66); nulliparity (OR = 6.17 and OR = 10.08), and multiparity with previous cesarean section (from OR = 5.73 to OR = 32.29). The presence of obstetric risk (OR = 1.94; 95%CI .44-2.62) also contributed to the occurrence of prelabor cesarean section in women who gave birth in the public service. CONCLUSIONS: AMA was an independent risk factor for prelabor cesarean in public and private services. In the public, prelabor cesarean in AMA was more influenced by clinical criteria. Higher chance of prelabor cesarean section in nulliparous women increases the chance of cesarean section in multiparous women, as we showed in this study, which increases the risk of anomalous placental implantation.

Childbirth care for adolescents and advanced maternal age in maternities linked to Rede Cegonha. / Assistência ao parto de adolescentes e mulheres em idade materna avançada em maternidades vinculadas à Rede Cegonha.

Based on the Rede Cegonha guidelines that propose the strengthening of women's sexual and reproductive rights, we sought to present a brief overview of issues related to reproductive planning and to analyze obstetric practices in childbirth care for adolescents and women of advanced age in maternity hospitals linked to Rede Cegonha. Data were extracted from an assessment conducted in 2017, based on information from the interview with puerperal women and from the hospital record. For age extremes, the high proportion of unplanned pregnancies and low use of contraception means problems in accessing family planning programs. Adolescents are more exposed to the presence of a companion and less to the use of analgesia in labor. Advanced maternal age were more likely to use analgesia in labor and to give birth in the lithotomy position, being less exposed to amniotomy. Although Rede Cegonha is an excellent strategy for improving assistance to childbirth, attention is still needed to the use of potentially unnecessary or not recommended interventions, with greater incentive to good obstetric practices.

Com base nas diretrizes da Rede Cegonha, que propõem fortalecimento dos direitos sexuais e dos direitos reprodutivos das mulheres, buscou-se apresentar um breve panorama de questões relacionadas ao planejamento reprodutivo e analisar as práticas obstétricas na atenção ao parto de adolescentes e mulheres em idade avançada, em maternidades vinculadas a Rede Cegonha. Os dados foram extraídos da avaliação conduzida em 2017, com base nas informações da entrevista com as puérperas e do prontuário hospitalar. Para os extremos etários, a alta proporção de gravidez não planejada e a baixa utilização de meios de contracepção indicam problemas no acesso aos programas de planejamento familiar. As adolescentes estão mais expostas à presença de acompanhante e menos ao uso de analgesia no trabalho de parto. As mulheres em idade avançada apresentaram maior chance de uso de analgesia no trabalho de parto e de parir na posição de litotomia, estando menos expostas a prática de amniotomia. Apesar da Rede Cegonha ser uma excelente estratégia para melhoria da assistência ao trabalho de parto e ao parto, ainda é preciso atenção ao uso de intervenções potencialmente desnecessárias ou não recomendadas, com maior incentivo às boas práticas obstétricas.

[Factors associated with educational guidance regarding oral health during prenatal care]. / Fatores associados à atividade educativa em saúde bucal na assistência pré-natal.

The scope of this article is to verify the factors associated with providing educational guidance related to oral health during prenatal care in the Metropolitan Region of Greater Vitória - ES, Brazil. It involved a cross-sectional study of 1,035 postpartum women in public or affiliated private hospitals in the SUS in 2010. A descriptive analysis of the main educational information and binomial logistic regression was conducted to test the association between individual and contextual variables and "receiving educational guidance through meetings and/or pregnant mother groups in the health unit and/or through home visits". The information given most during prenatal care was "the advantage of exclusive breastfeeding until six months of life" (52.0%) and least was "the importance of fluoride use in health" (18.9%). Being attended by the Family Health Strategy (FHS) (OR=1.94), having adequate living conditions for women and children (OR=1.73) and an additional prenatal visit (OR=1.08), increased the chance of providing educational guidance in prenatal care. Frequent pre-natal visits, coverage of health services in line with the FHS, and adequate conditions of housing were factors that were positively associated with educational guidance during prenatal care.

O objetivo deste artigo é verificar os fatores associados à realização de atividades educativas relacionadas à saúde bucal durante o acompanhamento pré-natal na Região Metropolitana da Grande Vitória-ES, Brasil. Estudo transversal com 1.035 puérperas em maternidades públicas ou conveniadas ao SUS, em 2010. Executou-se análise descritiva das principais informações educativas e regressão logística binomial associando-se variáveis individuais e contextuais com o desfecho "recebimento de atividade educativa por meio de reuniões e/ou grupo de gestante na unidade de saúde e/ou por meio de visita domiciliar". A informação mais recebida durante o pré-natal foi: "vantagem da amamentação exclusiva até os seis meses de vida" (52,0%) e a menos recebida foi "a importância do uso do flúor na saúde" (18,9%). Ter atendimento pela Estratégia Saúde da Família (OR=1,94), ter condições externas de moradia adequadas (OR=1,73) e aumento de 1 consulta pré-natal (OR=1,08) aumentaram a chance de realização de atividade educativa no pré-natal. Número elevado de consultas pré-natal, cobertura dos serviços de saúde nos moldes da ESF e condições externas de moradia adequadas foram fatores que se associaram positivamente às atividades educativas durante o pré-natal.

[Teenage pregnancy and prematurity in Brazil, 2011-2012]. / Prematuridade e gravidez na adolescência no Brasil, 2011-2012.

The study aims to evaluate the association between teenage pregnancy and prematurity. The data are from the Birth in Brazil study, a national survey consisting of 23,894 postpartum women and their newborn infants. The information was obtained from interviews with the mothers during their postpartum hospital stay. A matching method was established, based on propensity scores, to deal with differences between the groups due to the non-experimental design of the Birth in Brazil study. The study outcome was gestational age, considering all the premature births (gestational age < 37 weeks) and term births (gestational age 37 weeks to 41 weeks and 6 days). The study revealed social, economic, and maternal care disparities between the women according to age bracket. The highest proportions of teenage mothers were in the least developed regions of Brazil (North and Northeast) and in the poorest economic classes. After matching for socioeconomic and obstetric care characteristics, the highest odds of spontaneous prematurity were seen in younger adolescents compared to older adolescents (OR = 1.49; 95%CI: 1.07-2.06), and young adults (OR = 2.38; 95%CI: 1.82-3.12). Prematurity is still an issue in the field of maternal and child health, and the association with teenage pregnancy identified in this study is worrisome, especially because younger adolescent mothers were associated with higher odds of spontaneous prematurity.

Este trabalho tem como objetivo avaliar a associação entre gravidez na adolescência e prematuridade. Os dados são provenientes da pesquisa Nascer no Brasil, inquérito nacional composto por 23.894 puérperas e seus recém-nascidos. As informações foram obtidas por meio de entrevista com a puérpera durante a internação hospitalar. Um método de pareamento foi estabelecido, baseado nos escores de propensão, para lidar com diferenças entre os grupos em razão de um desenho não experimental, caso do estudo Nascer no Brasil. O desfecho do estudo foi a idade gestacional, sendo considerados todos os partos prematuros (idade gestacional < 37 semanas) e a termo (idade gestacional entre 37 e 41 semanas e 6 dias). O estudo evidenciou disparidades sociais, econômicas e assistenciais maternas entre as mulheres segundo a faixa etária. A maior proporção de puérperas adolescentes se concentrou nas regiões menos desenvolvidas do país, Norte e Nordeste, e nas classes econômicas menos favorecidas (D/E). Depois de equiparadas quanto às características socioeconômicas e assistenciais, foram observadas maiores chances de prematuridade espontânea nas adolescentes precoces, tanto em comparação às adolescentes tardias (OR = 1,49; IC95%: 1,07-2,06), quanto às adultas jovens (OR = 2,38; IC95%: 1,82-3,12). A prematuridade permanece em pauta no campo da saúde materno-infantil, sendo preocupante a associação com a gestação na adolescência encontrada neste trabalho, destacando-se que quanto mais jovem a gestante, maior a chance de parto prematuro espontâneo.

Este estudio tiene como objetivo evaluar la asociación entre embarazo en la adolescencia y prematuridad. Los datos provienen de la encuesta Nacer en Brasil, encuesta nacional compuesta por 23.894 puérperas y sus recién nacidos. La información se obtuvo mediante una entrevista con la puérpera durante el internamiento hospitalario. Se estableció un método de emparejamiento, basado en las puntuaciones de propensión, para tratar las diferencias entre los grupos, según un diseño no experimental, como es el caso del estudio Nacer en Brasil. El resultado del estudio fue la edad gestacional, considerándose todos los partos prematuros (edad gestacional < 37 semanas) y a término (edad gestacional entre 37 y 41 semanas y 6 días). El estudio evidenció disparidades sociales, económicas y asistenciales maternas entre las mujeres, según su franja de edad. La mayor proporción de puérperas adolescentes se concentró en las regiones menos desarrolladas del país, Norte y Nordeste, y en las clases económicas menos favorecidas (D/E). Tras equiparlas respecto a las características socioeconómicas y asistenciales, se observaron mayores oportunidades de prematuridad espontánea en las adolescentes precoces, tanto en comparación con las adolescentes tardías (OR = 1,49; IC95%: 1,07-2,06), como con las adultas jóvenes (OR = 2,38; IC95%: 1,82-3,12). La prematuridad permanece en pauta en el campo de la salud materno-infantil, siendo preocupante la asociación con la gestación en la adolescencia hallada en este estudio, destacándose que cuanto más joven es la gestante, mayor es la oportunidad de parto prematuro espontáneo.

Oral health and quality of life of pregnant women: the influence of sociodemographic factors. / Saúde bucal e qualidade de vida de gestantes: a influência de fatores sociais e demográficos.

This study aimed to evaluate the relationship between sociodemographic factors and the impact on Oral Health-Related Quality of Life (OHR-QoL) in Brazilian pregnant women users of the Unified Health System. This is a cross-sectional epidemiological study developed with pregnant women living in two regions with different sociodemographic characteristics. In total, 1,777 puerperae were interviewed. A structured and previously tested questionnaire collected sociodemographic variables, and the Oral Health Index Profile (OHIP-14) assessed the impact on the OHR-QoL. The statistical analysis was performed using the Chi-square test and multiple logistic regression, both with a significance of 5%. The "psychological discomfort" realm was the only one with a difference between the puerperae of the RMGV and the MRSM (p=0.042). The following variables were associated with the impact on the OHR-QoL: residing in the RMGV (OR=1.69; 95%CI: 1.16-2.47); having a low level of schooling (OR=1.80; 95%CI: 1.03-3.18) and visit to the dentist during pregnancy (OR=2.15, 95%CI: 1.50-3.07). Sociodemographic factors should be considered in the planning of oral health actions of pregnant women, as they influence the impact on the OHR-QoL.

Este estudo buscou avaliar a relação entre fatores sociodemográficos e o impacto na Qualidade de Vida Relacionada à Saúde Bucal (QVRSB), em gestantes brasileiras usuárias do Sistema Único de Saúde. Trata-se de um estudo epidemiológico observacional transversal, desenvolvido com gestantes residentes em duas regiões com características sociodemográficas distintas. Foram entrevistadas 1.777 puérperas. Um questionário estruturado e previamente testado coletou as variáveis sociodemográficas e o Oral Health Index Profile (OHIP-14) avaliou o impacto na QVRSB. A análise estatística foi realizada utilizando o teste Qui-quadrado e a regressão logística múltipla, ambos com significância de 5%. A dimensão "desconforto psicológico" foi a única com diferença entre as puérperas da RMGV e MRSM (p=0,042). Associou-se ao impacto na QVRSB as variáveis: residir na RMGV (OR=1,69; IC95%: 1,16-2,47); ter pouca escolaridade (OR=1,80; IC95%: 1,03-3,18) e realizar consulta odontológica durante a gestação (OR=2,15; IC95%: 1,50-3,07). Os fatores sociodemográficos devem ser considerados no planejamento das ações em saúde bucal das gestantes, pois influenciam no impacto na QVRSB.

Introduction of human gammaherpesvirus 8 genotypes A, B, and C into Brazil from multiple geographic regions.

Variations in the open reading frame (ORF) K1 gene sequence of human gammaherpesvirus 8 (HHV-8) has led to the identification of 6 major genotypic clades (A, B, C, D, E, and F) in specimens isolated from around the world. These clades exhibit clear clustering among individuals in different ethnic groups and from different geographic regions. The human population of Brazil varies greatly in ethnicity because of multiple immigration events from Africa, Europe, Asia, and indigenous communities. However, there is scant information about the HHV-8 genotypes currently circulating in Brazil. Here, we describe HHV-8 genotypic diversity in isolates from Brazilian HIV-infected patients living with Kaposi's sarcoma (KS) by analysis of the complete ORF-K1 region. We also identified the most likely geographic origins of these different Brazilian genotypes. We extracted HHV-8 DNA (24 positive samples) from individuals with HIV/KS from the states of São Paulo and Rio de Janeiro, amplified the ORF-K1 gene using nested PCR (about 870 base pairs), performed sequencing and phylogenetic analysis, and then calculated the mean genetic distances of Brazilian sequences from sequences in other regions of the world (523 sequences analyzed). Phylogenetic analysis showed that genotypes C, A, and B were present in 45.8 %, 29.2 % and 25 % of the isolates from Brazil, respectively. These isolates grouped into separate clades, rather than a single monophyletic cluster. Mean genetic distance analyses suggested that these genotypes were introduced into the Brazil multiple times from different geographical regions. HHV-8/A isolates appear to be from Ukraine, Russia, and the Tartar ethnic group; HHV-8/B isolates appear to be from Congo and Democratic Republic of the Congo; and HHV-8/C isolates appear to be from Australia, Algeria, England, and French Guiana. These results contribute to a better understanding of the genetic diversity and origins of HHV-8 strains circulating in Brazil, and will provide a foundation for further epidemiological and evolutionary studies of HHV-8.

Cytokine, Genotype, and Viral Load Profile in the Acute and Chronic Hepatitis B.

Several hepatitis B virus (HBV) factors, including viral load, genotype, genome mutations, and cytokine production, have been reported to be associated with different risks of progression of liver disease. The aim of this study was to verify if there is an association among the levels of cytokines (interleukin [IL]-35, IL-6, IL-17A, interferon [IFN]-γ) in the plasma, viral load, and the different genotypes of HBV in patients with acute or chronic hepatitis B. Methods: 49 serum samples, 20 from acute and 29 from chronic cases, were submitted to a real-time and nested-polymerase chain reaction to quantify, detect, and genotype HBV DNA. The cytokines IL-35, IL-6, IL-17A, and IFN-γ were detected by an enzyme-linked immunosorbent assay (ELISA). The median viral load was 3.15 log10 IU DNA/mL and 2.90 log10 IU DNA/mL for acute and chronic patients, respectively. Genotype A, D, E, and F were identified in chronic carriers of HBV infection, while only genotype A and F were identified in individuals with acute infection. IFN-γ (p = 0.024) and IL-17A (p = 0.046) levels were significantly increased in chronic patients and IL-6 and IL-35 were higher in patients with acute infection, however, without statistical difference. IL-17A and IFN-γ can be modulating proinflammatory effects and inducing hepatocellular damage, in chronic patients, and IL-6 and IL-35 may be involved in viral elimination and protection against chronicity during the acute phase of infection. These results can contribute to understanding of the complex regulatory mechanisms of the host antiviral response related to cytokine production during acute and chronic HBV infection.

Gestational risk and social inequalities: a possible relationship? / Risco gestacional e desigualdades sociais: uma relação possível?

This study aims to analyze the association between social inequalities and gestational risk in administrative regions of the state of Espírito Santo. This is a cross-sectional study based on two administrative regions of the state of Espírito Santo. The sample consisted of 1,777 women who underwent prenatal care in the municipalities of the Greater Vitória Metropolitan Region (RMGV-ES) and São Mateus Microregion (MRSM) and were admitted to the public health facilities at the time of delivery between 2010 and 2012/2013. The multivariate logistic regression was performed to test the association between social and gestational risk variables. Variables with a significance level < 0.20 in the Chi-square test were adopted for the final model, and only those variables with a p-value < 0.05 remained. An association was found between high gestational risk and women's dwelling place in the RMGV-ES (OR = 1.74; CI95% 1.32-2.28), women as head of households (OR = 3.03; CI95% 1.64-5.61), head of household with less than five years of schooling (OR = 1.58; CI95% 1.14-2.20) and receipt of social benefit "Bolsa Família" (Family Grant) (OR = 1.46; CI95% 1.04-2.03). While some social variables underpin the classification of gestational risk, other social factors have been shown to produce this risk.

O objetivo do estudo é analisar a associação entre as desigualdades sociais e o risco gestacional em regiões administrativas do estado do Espírito Santo. Estudo transversal, com base em duas regiões administrativas do estado do Espírito Santo. A amostra foi composta por 1.777 mulheres que realizaram o pré-natal nos municípios da Região Metropolitana da Grande Vitória (RMGV-ES) e Microrregião de São Mateus internadas nos estabelecimentos da rede pública de saúde, por ocasião do parto, entre 2010 e 2012/2013. Realizou-se Regressão Logística Multivariada para testar a associação entre variáveis sociais e risco gestacional. As variáveis que apresentaram nível de significância menor que 20% no teste Qui-quadrado foram utilizadas para o modelo final, permanecendo aquelas com significância menor que 5%. Encontrou-se associação entre alto risco gestacional e o local de moradia da mulher na RMGV-ES (OR = 1,74; IC 95% 1,32-2,28), a chefia da mulher na família (OR = 3,03; IC 95% 1,64-5,61), a escolaridade da chefia familiar menor que cinco anos (OR = 1,58; IC 95% 1,14-2,20) e o recebimento do benefício social "Bolsa Família" (OR = 1,46; IC 95% 1,04-2,03). Apesar de algumas variáveis sociais comporem a classificação do risco gestacional, outros fatores sociais revelaram-se como produtores do risco gestacional.