Manipulation of the seagrass-associated microbiome reduces disease severity.

Host-associated microbes influence host health and function and can be a first line of defence against infections. While research increasingly shows that terrestrial plant microbiomes contribute to bacterial, fungal, and oomycete disease resistance, no comparable experimental work has investigated marine plant microbiomes or more diverse disease agents. We test the hypothesis that the eelgrass (Zostera marina) leaf microbiome increases resistance to seagrass wasting disease. From field eelgrass with paired diseased and asymptomatic tissue, 16S rRNA gene amplicon sequencing revealed that bacterial composition and richness varied markedly between diseased and asymptomatic tissue in one of the two years. This suggests that the influence of disease on eelgrass microbial communities may vary with environmental conditions. We next experimentally reduced the eelgrass microbiome with antibiotics and bleach, then inoculated plants with Labyrinthula zosterae, the causative agent of wasting disease. We detected significantly higher disease severity in eelgrass with a native microbiome than an experimentally reduced microbiome. Our results over multiple experiments do not support a protective role of the eelgrass microbiome against L. zosterae. Further studies of these marine host-microbe-pathogen relationships may continue to show new relationships between plant microbiomes and diseases.

Macroplastic accumulation in roadside ditches of New York State's Finger Lakes region (USA) across land uses and the COVID-19 pandemic.

Macroplastics are a ubiquitous and growing environmental contaminant with impacts in both marine and terrestrial systems. Marine sampling has dominated research in this field, despite the terrestrial origins of most plastic debris. Due to the high surface water connectivity facilitated by roadside ditches, these landscape features provide a unique sampling location linking terrestrial and surface water systems. We collected and analyzed macroplastic accumulation by number of pieces, mass, and polymer type in roadside ditches across four land uses, before and during the COVID-19 pandemic in the Finger Lakes Region of New York State. Commercial land use plastic accumulation rate was highest, while forested land use accumulation rates were lowest on a piece basis. Pre-COVID-19 piece accumulation rates were significantly higher than COVID-19 piece accumulation rates across all land uses. Mass accumulation rates followed similar patterns observed in piece accumulation, but the patterns were not always statistically significant. Plastic type 4 (i.e. thin plastic films), especially plastic bags and wrappers, was the most frequently collected type of macroplastic by piece across all land uses within the 1-7 Resin Identification Codes. By mass, the data were distributed less consistently across land uses. Cigarette filters, containing the polymer cellulose acetate, were the most frequently found roadside plastic, but are not within the 1-7 classification system. Our results suggest that policies in place limiting plastic bag usage could substantially reduce roadside plastics but other plastics, such as food wrappers and other single use plastic films, which comprised a large proportion of the plastic debris collected, should also be regulated to further decrease macroplastic pollution.

Roadside ditch macroplastic and other litter dataset in the Finger lakes region across land uses and COVID-19 pandemic.

Litter was collected from 12 roadside ditches in the Finger lakes Region of New York State over two sampling periods: pre-COVID-19 pandemic and during COVID-19 pandemic. Litter pieces were washed in DI water, oven dried, massed and plastic-type visually determined. Macroplastic data was analysed to assess the impact of land use, traffic, and COVID-19 variables on macroplastic accumulation on a piece, mass, and plastic-type basis. These data are all litter pieces collected, including both plastics categorized as 1 through 7 in the RIC resin classification codes as well as non-plastic litter. These data have wide-ranging reuse potential, as terrestrial microplastic accumulation is not well documented. These data could be compared with other litter accumulation across regions, specifically to assess total environmental macroplastic loading and enable contaminant mitigation strategies. These data also have direct application to modelling and transport of macroplastics into surface water bodies as a result of road ditch sampling locations. Macroplastic accumulation data across varying land uses, traffic, and COVID-19 conditions has been published [1].

Plasma-modified ACT can be used to monitor bivalirudin (Angiomax) anticoagulation for on-pump cardiopulmonary bypass surgery in a patient with heparin-induced thrombocytopenia.

Heparin-induced thrombocytopenia (HIT) is a problem of growing significance and concern, affecting an estimated 1-3% of patients among those receiving an estimated 10 million heparin exposures annually in the United States. The prevalence of heparin antibodies has been reported as at least 12.7% in the general cardiac surgery population before surgery and 42% following surgery. Various management techniques for anticoagulation in these patients have been proposed. Many of these alternative agents present additional risks for bleeding and efficacy and, furthermore, require the use of nonconventional monitoring assays to assess the level of anticoagulation achieved adequately. We report here the successful use of bivalirudin anticoagulation on pump, with no additional morbidity, and the first reported use of the plasma-modified ACT (activated clotting time) test, a simple modification of the standard ACT assay, to monitor the anticoagulant effect of bivalirudin.