RESUMEN
The ε-subunit of ATP-synthase is an endogenous inhibitor of the hydrolysis activity of the complex and its α-helical C-terminal domain (εCTD) undergoes drastic changes among at least two different conformations. Even though this domain is not essential for ATP synthesis activity, there is evidence for its involvement in the coupling mechanism of the pump. Recently, it was proposed that coupling of the ATP synthase can vary as a function of ADP and Pi concentration. In the present work, we have explored the possible role of the εCTD in this ADP- and Pi-dependent coupling, by examining an εCTD-lacking mutant of Escherichia coli. We show that the loss of Pi-dependent coupling can be observed also in the εCTD-less mutant, but the effects of Pi on both proton pumping and ATP hydrolysis were much weaker in the mutant than in the wild-type. We also show that the εCTD strongly influences the binding of ADP to a very tight binding site (half-maximal effect≈1nM); binding at this site induces higher coupling in EFOF1 and increases responses to Pi. It is proposed that one physiological role of the εCTD is to regulate the kinetics and affinity of ADP/Pi binding, promoting ADP/Pi-dependent coupling.
Asunto(s)
Adenosina Difosfato/metabolismo , Adenosina Trifosfato/metabolismo , Escherichia coli/metabolismo , Bombas de Protones/metabolismo , ATPasas de Translocación de Protón/metabolismo , Adenosina Difosfato/análogos & derivados , Sitios de Unión/fisiología , Hidrólisis , Cinética , Dominios Proteicos/fisiología , ProtonesRESUMEN
The nonsulfur purple photosynthetic bacterium Rhodopseudomonas capsulata can obtain energy for growth either by anaerobic photophosphorylation or dark oxidative (aerobic) phosphorylation. Successful resolution of phosphorylation coupling factors from energy-converting membranes of this bacterium permitted tests for reciprocal function of such protein factors in oxidative-and photophosphorylation processes. Evidence was obtained for the interchangeability of coupling factor preparations from dark-grown and photosynthetically grown cells in both kinds of energy conversion.
Asunto(s)
Transferencia de Energía , Fosforilación Oxidativa , Fotosíntesis , Rhodopseudomonas/metabolismo , Aerobiosis , Anaerobiosis , Reacciones Antígeno-Anticuerpo , Oscuridad , NADP/farmacología , Consumo de Oxígeno , Isótopos de Fósforo , Succinatos/farmacologíaRESUMEN
1. In membranes prepared from dark grown cells of Rhodopseudomonas capsulata, five cytochromes of b type (E'0 at pH 7.0 +413+/-5, +270+/-5, +148+/-5, +56+/-5 and -32+/-5 mV) can be detected by redox titrations at different pH values. The midpoint potentials of only three of these cytochromes (b148, b56, and b-32) vary as a function of pH with a slope of 30 mV per pH unit. 2. In the presence of a CO/N2 mixture, the apparent E'0 of cytochrome b270 shifts markedly towards higher potentials (+355mV); a similar but less pronounced shift is apparent also for cytochrome b150. The effect of CO on the midpoint potential of cytochrome b270 is absent in the respiration deficient mutant M6 which possesses a specific lesion in the CO-sensitive segment of the branched respiratory chain present in the wild type strain. 3. Preparations of spheroplasts with lysozyme digestion lead to the release of a large amount of cytochrome c2 and of virtually all cytochrome cc'. These preparations show a respiratory chain impaired in the electron pathway sensitive to low KCN concentration, in agreement with the proposed role of cytochrome c2 in this branch; on the contrary, the activity of the CO-sensitive branch remains unaffected, indicating that neither cytochrome c2 nor the CO-binding cytochrome cc' are involved in this pathway. 4. Membranes prepared from spheroplasts still possess a CO-binding pigment characterized by maxima at 420.5, 543 and 574 nm and minima at 431, 560 nm in C0-difference spectra and with an alpha band at 562.5 nm in reduced minus oxidized difference spectra. This membrane-bound cytochrome, which is coincident with cytochrome b270, can be classified as a typical cytochrome "0" and considered the alternative CO-sensitive oxidase.
Asunto(s)
Citocromos/metabolismo , Complejo IV de Transporte de Electrones/metabolismo , Consumo de Oxígeno , Fotosíntesis , Rhodopseudomonas/enzimología , Anaerobiosis , Monóxido de Carbono/farmacología , Cianuros/farmacología , Ditionita , Concentración de Iones de Hidrógeno , Luz , Mutación , Oxidación-Reducción , Potenciometría , Especificidad de la Especie , EspectrofotometríaRESUMEN
ATPase activity and ATP-induced energization of photosynthetic membranes from Rhodopseudomonas capsulata are stimulated by phosphate; the maximum stimulatory effect occurs at a concentration between 1 and 2 mM. The sensitivity of the ATPase to oligomycin increases in the presence of phosphate since all the Pi-stimulated activity is inhibited by this antibiotic. Aurovertin, which has no effect on ATPase in the absence of phosphate, inhibits completely the activity elicited by this anion. The addition of Pi induces a substantial increase in the V of ATPase activity without changing the affinity of the enzyme for ATP or ADP. Arsenate, at the same concentrations, produces effects very similar to those of phosphate. The stimulation by arsenate of the transfer of energy from ATP to the membrane suggests a non-hydrolytic role of this anion as a modifier of the ATPase activity.
Asunto(s)
Adenosina Trifosfatasas/metabolismo , Fosfatos/farmacología , Fotofosforilación/efectos de los fármacos , Rhodopseudomonas/metabolismo , Adenosina Difosfato/farmacología , Antimetabolitos/farmacología , Arseniatos/farmacología , Transferencia de Energía , Activación Enzimática/efectos de los fármacos , Hidrazonas/farmacología , Cinética , Luz , Nitrilos/farmacología , Oligomicinas/farmacología , Rhodopseudomonas/efectos de los fármacosRESUMEN
The respiratory chain of Rhodopseudomonas capsulata, strain St. Louis and of two respiration deficient mutants (M6 and M7) has been investigated by examining the redox and spectral characteristics of the cytochromes and their response to substrates and to specific respiratory inhibitors. Since the specific lesions of M6 and M7 have been localized on two different branches of the multiple oxidase system of the wild type strain, the capability for aerobic growth of these mutants can be considered as a proof of the physiological significance of both branched systems "in vivo". Using M6 and M7 mutants the response of the branched chain to respiratory inhibitors could be established. Cytochrome oxidase activity, a specific function of an high potential cytochrome b (E'0 = +413 mV) is sensitive to low concentrations of KCN (5-10(-5) M); CO is a specific inhibitor of an alternative oxidase, which is also inhibited by high concentrations of KCN (10(-3) M). Antimycin A inhibits preferentially the branch of the chain affected by low concentrations of cyanide. Redox titrations and spectral data indicate the presence in the membrane of three cytochromes of b type (E'0 = +413, +260, +47 vM) and two cytochromes of c type (E'0 = +342, +94 mV). A clear indication of the involvement in respiration of cytochrome b413, cytochrome c342 and cytochrome b47 has been obtained. Only 50% of the dithionite reducible cytochrome b can be reduced by respiratory substrates also in the presence of high concentrations of KCN or in anaerobiosis. The presence and function of quinones in the respiratory electron transport system has been clearly demonstrated. Quinones, which are reducible by NADH and succinate to about the same extent can be reoxidized through both branches of the respiratory chain, as shown by the response of their redox state to KCN. The possible site of the branching of the electron transport chain has been investigated comparing the per cent level of reduction of quinones and of cytochromes b and c as a function of KCN concentrations in membranes from wild type and M6 mutants cells. The site of the branching has been localized at the level of quinones-cytochrome b47. A tentative scheme of the respiratory chains operating in Rhodopseudomonas capsulata, St. Louis and in the two respiration deficient mutants, M6 and M7 is presented.
Asunto(s)
Complejo IV de Transporte de Electrones/metabolismo , Transferencia de Energía , Rhodopseudomonas/enzimología , Anaerobiosis , Antimicina A/farmacología , Cianuros/farmacología , Citocromos/metabolismo , Transporte de Electrón/efectos de los fármacos , Transferencia de Energía/efectos de los fármacos , Membranas/enzimología , Membranas/efectos de la radiación , NADH NADPH Oxidorreductasas/metabolismo , Oxidación-Reducción , Consumo de Oxígeno/efectos de los fármacos , Consumo de Oxígeno/efectos de la radiación , Fotosíntesis/efectos de los fármacos , Quinonas/metabolismo , Efectos de la Radiación , Rhodopseudomonas/metabolismo , Rhodopseudomonas/efectos de la radiación , Espectrofotometría Ultravioleta , Succinatos/metabolismo , Rayos UltravioletaRESUMEN
Ubiquinone-10 can be extracted from lyophilized chromatophores of Rhodobacter sphaeroides (previously called Rhodopseudomonas sphaeroides) without significant losses in other components of the electron-transfer chain or irreversible damages in the membrane structure. The pool of ubiquinone can be restored with exogenous UQ-10 to sizes larger than the ones in unextracted membranes. The decrease in the pool size has marked effects on the kinetics of reduction of cytochrome b-561 induced by a single flash of light and measured in the presence of antimycin. The initial rate of reduction, which in unextracted preparations increases on reduction of the suspension over the Eh range between 170 and 100 mV (pH 7), is also stimulated in partially UQ-depleted membranes, although at more negative Eh's. When the UQ pool is completely extracted the rate of cytochrome (Cyt) b-561 reduction is low and unaffected by the redox potential. In membranes enriched in UQ-10 above the physiological level the titration curve of the rate of Cyt b-561 reduction is displaced to Eh values more positive than in controls. This effect is saturated when the size of the UQ pool is about 2-3 times larger than the native one. The reduction of Cyt b-561 always occurs a short time after the flash is fired; also the duration of this lag is dependent on Eh and on the size of the UQ pool. A decrease or an increase in the pool size causes a displacement of the titration curve of the lag to more negative or to more positive Eh's, respectively. Similarly, the lag becomes Eh independent and markedly longer than in controls when the pool is completely extracted. These results demonstrate that the rate of turnover of the ubiquinol oxidizing site in the b-c1 complex depends on the actual concentration of ubiquinol present in the membrane and that ubiquinol from the pool is oxidized at this site with a collisional mechanism. Kinetic analysis of the data indicates that this reaction obeys a Michaelis-Menten type equation, with a Km of 3-5 ubiquinol molecules per reaction center.
Asunto(s)
Complejo III de Transporte de Electrones/metabolismo , Fotosíntesis , Rhodopseudomonas/metabolismo , Ubiquinona/análogos & derivados , Cromatóforos Bacterianos/metabolismo , Grupo Citocromo b/metabolismo , Transporte de Electrón , Cinética , Oxidación-Reducción , Ubiquinona/aislamiento & purificación , Ubiquinona/metabolismoRESUMEN
We analyze the adsorption of the fluorescent monoamine 9-aminoacridine to the membrane phase of photosynthetic chromatophores, in the physiological interval of pH values ranging from 5.5 to 8.5 and at ionic strengths of 0.005 and 0.150 M. The interaction of the probe with the membrane phase is described with S-shaped isotherms of the Hill type and is modulated by electrostatic effects as modelled with the Gouy-Chapman-Boltzman theory. This description is consistent with different values of the surface change density of the chromatophore membranes decreasing from about 1.3 x 10(-3) to about 0.5 x 10(-3) e-/A2, on changing the pH from 8.5/7.5 to 6.5/5.5, respectively. Furthermore we show that, when the free concentrations of the probe in the inner and outer vesicle compartments are computed from the adsorbing isotherms at the proper pH values, the model considering the equilibrium distribution of the neutral monoamine following the onset of a delta pH is sufficient to describe the dependence of the artificially induced transmembrane delta pH values on the observed quenching of the probe fluorescence.
Asunto(s)
Aminacrina/química , Membrana Celular/química , Cromatóforos/química , Concentración de Iones de Hidrógeno , Colorantes Fluorescentes , MicrodiálisisRESUMEN
N-Ethylmaleimide, at millimolar concentrations, irreversibily inhibits photophosphorylation and ATPase activity of photosynthetic membranes from Rhodopseudomonas capsulata. The inhibitory effect of N-ethylmaleimide is evident only the membranes are preincubated with the inhibitor in the light and in the absence of phosphorylation substrates. ADP and orthophosphate (or arsenate) exert a protective effect against the inhibition if they are present during the preillumination stage. The energization of the membrane by ATP hydrolysis, measured as ATP-induced quenching of 9-aminoacridine fluorescence, also is inhibited irreversibly by N-ethylmaleimide. Uncouplers protect the ATPase from inhibition by N-ethylmaleimide at concentrations at which they inhibit photophosphorylation. The ATPase, as measured either in the dark or in the light, is also inhibited by carbonylcyanide p-trifluoromethoxypenylhydrazone in parallel with photophosphorylation. These results are interpreted as evidence that the high-energy state of the membrane induces a conformational change of the ATPase, making it sensitive to attack by N-ethylmaleimide; this conformational change might be related to the active state of the ATPase.
Asunto(s)
Adenosina Trifosfatasas/antagonistas & inhibidores , Etilmaleimida/farmacología , Fotofosforilación/efectos de los fármacos , Rhodopseudomonas/metabolismo , Adenosina Difosfato/farmacología , Transferencia de Energía , Hidrazonas/farmacología , Luz , Membranas/efectos de los fármacos , Membranas/metabolismo , Nitrilos/farmacología , Fosfatos/farmacología , Rhodopseudomonas/efectos de los fármacosRESUMEN
(1) When chromatophores from Rhodopseudomonas capsulata Ala pho+ are exposed to a train of high-frequency, saturating flashes the kinetics of the reaction centre bacteriochlorophyll absorption change enter a pseudo steady-state in which the extent of oxidation during the flashes is equal to the extent of reduction in between the flashes. The level of the pseudo steady-state is lowered by the presence of a phosphate acceptor system, raised by further addition of oligomycin, lowered by a combination of nigericin and valinomycin and raised by antimycin A. (2) In the pseudo steady-state, the extent of reaction centre bacteriochlorophyll oxidation taking place during the flash may be estimated by subtraction from the total concentration of reaction centre bacteriochlorophyll. This value is equated with the amount of electrons transported through the photosynthetic chain. Comparison with the measured ATP yield per flash in the pseudo steady-state permits calculation of the ATP: two electron ratio. The value of the ratio is 1.1 for flash frequencies between 3 and 12.5 Hz and declines at lower and higher frequencies. The ATP: two electron ratio is approximately halved in the presence of antimycin A. (3) An alternative estimate of the ATP: two electron ratio, based on the assumption that high-frequency flashes approximate to the condition of continuous illumination, was approx. 0.8.
Asunto(s)
Adenosina Trifosfato/metabolismo , Cromatóforos Bacterianos/metabolismo , Fotosíntesis/efectos de la radiación , Rhodopseudomonas/metabolismo , Antimicina A/farmacología , Bacterioclorofilas/metabolismo , Transporte de Electrón/efectos de los fármacos , Electrones , Cinética , Luz , Oxidación-ReducciónRESUMEN
1. ATP synthesis (monitored by luciferin-luciferase) can be elicited by a single turnover flash of saturating intensity in chromatophores from Rhodopseudomonas capsulata, Kb1. The ATP yield from the first to the fourth turnover is strongly influenced by the phosphate potential: at high phosphate potential (-11.5 kcal/mol) no ATP is formed in the first three turnovers while at lower phosphate potential (-8.2 kcal/mol) and the yield in the first flash is already one half of the maximum, which is reached after 2-3 turnovers. 2. The response to ionophores indicates that the driving force for ATP synthesis in the first 20 turnovers is mainly given by a membrane potential. The amplitude of the carotenoid band shift shows that during a train of flashes an increasing delta psi is built up, which reaches a stationary level after a few turnovers; at high phosphate potential, therefore, more turnovers of the same photosynthetic unit are required to overcome an energetic threshold. 3. After several (six to seven) flashes the ATP yield becomes constant, independently from the phosphate potential; the yield varies, however, as a function of dark time (td) between flashes, with an optimum for td = 160-320 ms. 4. The decay kinetics of the high energy state generated by a long (125 ms) flash have been studied directly measuring the ATP yield produced in post-illumination by one single turnover flash, under conditions of phosphate potential (-10 kcal/mol), which will not allow ATP formation by one single turnover. The high energy state decays within 20 s after the illumination. The decay rate is strongly accelerated by 10(-8) M valinomycin. 5. Under all the experimental conditions described, the amplitude of the carotenoid signal correlates univocally with the ATP yield per flash, demonstrating that this signal monitores accurately an energetic state of the membrane directly involved in ATP synthesis. 6. Although values of the carotenoid signal much larger than the minimal threshold are present, relax slowly, and contribute to the energy input for phosphorylation, no ATP is formed unless electron flow is induced by a single turnover flash. 7. The conclusions drawn are independent from the assumption that a delta psi between bulk phases is evaluable from the carotenoid signal.
Asunto(s)
Carotenoides/metabolismo , Fotofosforilación , Rhodopseudomonas/metabolismo , Adenosina Trifosfato/biosíntesis , Cromatóforos Bacterianos/metabolismo , Mediciones Luminiscentes , Nigericina/farmacología , Fotofosforilación/efectos de los fármacos , Espectrofotometría , Valinomicina/farmacologíaRESUMEN
Monospecific antibodies have been prepared against cytochrome c2 from Rhodopseudomonas spheroides and Rhodopseudomonas capsulata, and against cytochrome c' from Rps. capsulata. These antibodies precipitated their respective antigens, but did not cross react with a wide range of procaryotic or eucaryotic cytochromes, or with other bacterial proteins. The cytochromes produced during aerobic growth were immunologically indistinguishable from those produced during photosynthetic growth. Cytochrome c2 is located in vivo in the periplasmic space between the cell was and the cell membrane, and when chromatophores are prepared from whole cells the cytochrome becomes trapped inside these vesicles. The implications of these results to energy coupling in the photosynthetic bacteria are discussed.
Asunto(s)
Grupo Citocromo c/análisis , Rhodobacter sphaeroides/enzimología , Rhodopseudomonas/enzimología , Animales , Reacciones Antígeno-Anticuerpo , Membrana Celular/enzimología , Reacciones Cruzadas , Transporte de Electrón , Transferencia de Energía , Inmunodifusión , Inmunoelectroforesis , Modelos Biológicos , Oxidación-Reducción , Unión Proteica , Conejos/inmunología , Especificidad de la EspecieRESUMEN
Experimental data are reviewed that are not in keeping with the scheme of 'delocalized' protonic coupling in membrane-linked free-energy transduction. It turns out that there are three main types of anomalies: (i) rates of electron transfer and of ATP synthesis do not solely depend on their own driving force and on delta mu H, (ii) the ('static head') ratio of delta Gp to delta mu H varies with delta mu H and (iii) inhibition of either some of the electron-transfer chains or some of the H+-ATPases, does not cause an overcapacity in the other, non-inhibited proton pumps. None of the earlier free-energy coupling schemes, alternative to delocalized protonic coupling, can account for these three anomalies. We propose to add a fifth postulate, namely that of the coupling unit, to the four existing postulates of 'delocalized protonic coupling' and show that, with this postulate, protonic coupling can again account for most experimental observations. We also discuss: (i) how experimental data that might seem to be at odds with the 'coupling unit' hypothesis can be accounted for and (ii) the problem of the spatial arrangement of the electrical field in the different free-energy coupling schemes.
Asunto(s)
Membranas/metabolismo , Complejos Multienzimáticos/metabolismo , Fosfotransferasas/metabolismo , Complejos de ATP Sintetasa , Adenosina Trifosfato/metabolismo , Transporte de Electrón , Concentración de Iones de Hidrógeno , Cinética , Modelos Biológicos , Fosforilación , TermodinámicaRESUMEN
A quantitative study of the kinetics of electron transfer under coupled conditions in photosynthetic bacteria has so far been prevented by overlap of the electrochromic signals of carotenoids and bacteriochlorophyll with the absorbance changes of cytochromes and reaction centers. In this paper a method is presented by which the electrochromic contribution at any wavelength can be calculated from the electrochromic signal recorded at 505 nm, using a set of empirically determined polynomial functions. The electrochromic contribution to kinetic changes at any wavelength can then be subtracted to leave the true kinetics of the redox changes. The corrected redox changes of the reaction center measured at 542 and 605 nm mutually agree, thus providing an excellent test of self-consistency of the method. The corrected traces for reaction center and of cytochrome b-566 demonstrate large effects of the membrane potential on the rate and poise of electron transfer. It will be possible to study the interrelation between proton gradient and individual electron reactions under flash or steady-state illumination.
Asunto(s)
Cromatóforos Bacterianos/metabolismo , Rhodopseudomonas/metabolismo , Cromatóforos Bacterianos/efectos de los fármacos , Grupo Citocromo b/metabolismo , Oscuridad , Transporte de Electrón , Cinética , Luz , Oxidación-Reducción , Fotosíntesis , Valinomicina/farmacologíaRESUMEN
ATP hydrolysis induces the activation of the proton ATPase in chromatophores of Rhodobacter capsulatus supplemented with nigericine and 50 mM K+ (i.e. when delta pH < 0.2 units). The value of transmembrane electric potential (delta phi) driving this activation was measured using three different approaches: carotenoid electrochromism, uptake of SCN- and responses of the dye oxonol VI. The value of delta phi calculated from the SCN- uptake, on the basis of an internal volume determined experimentally, was about 140 mV, while that indicated by the electrochromic signal ranged between 35 and 70 mV. Only the value indicated by SCN- distribution is consistent with the energetic requirement for the activation of H(+)-ATPase.
Asunto(s)
Adenosina Trifosfato/metabolismo , Carotenoides/metabolismo , ATPasas de Translocación de Protón/metabolismo , Rhodobacter capsulatus/metabolismo , Cromatóforos Bacterianos/metabolismo , Transporte Biológico , Activación Enzimática , Hidrólisis , Luz , Potenciales de la Membrana , Tiocianatos/metabolismoRESUMEN
The phospholipids and the fatty acids present in membranes of cells of Rhodopseudomonas capsulata, grown photosynthetically in anaerobiosis, were analyzed by thin layer chromatography and gas chromatography-mass spectrometry. The three phospholipids detected, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol, contained about 80% of a single monounsaturated C18 fatty acid, cis-vaccenic acid. These membranes offer therefore a naturally occurring model system endowed with an extremely simplified phospholipid complement. The data indicate moreover that the biosynthetic pathway of unsaturated fatty acids present in these facultative aerobic bacteria proceeds only via the 3-hydroxydecanoyl acyl carrier protein dehydratase (E.C. 4.2.1.60).