RESUMEN
The high fidelity observed in biological information processing ranging from replication to translation has stimulated significant research efforts to clarify the underlying microscopic picture. Theoretically, several approaches to analyze the error rates have been proposed. The copolymerization theory describes the addition and removal of monomers at the growing tip of a copolymer, leading to a closed set of nonlinear equations. On the other hand, enzyme-kinetics approaches formulate linear equations of biochemical networks, describing transitions between discrete chemical states. However, it is still unclear whether the error values computed by the two approaches agree. Moreover, there are conflicting interpretations on whether the error is under thermodynamic or kinetic discrimination control. In this work, we examine the error rate in persistent copying biochemical processes by specifically analyzing both theoretical approaches. The initial disagreement of the results between the two theories motivated us to rederive the formula for the error rate in the kinetic model. The error computed with the new method resulted in excellent agreement between both theoretical approaches and with Monte Carlo simulations. Furthermore, our theoretical analysis shows that the kinetic discrimination controls the error, even when the energy difference between adding the right and wrong products is very small. Our theoretical investigation gives important insights into the physical-chemical properties of complex biological processes by providing the quantitative framework to evaluate them.
Asunto(s)
Enzimas , Método de Montecarlo , Polimerizacion , Cinética , Enzimas/metabolismo , Enzimas/química , TermodinámicaRESUMEN
Biological processes exhibit remarkable accuracy and speed and can be theoretically explored through various approaches. The Markov-chain copolymerization theory, describing polymer growth kinetics as a Markov chain, provides an exact set of equations to solve for error and speed. Still, due to nonlinearity, these equations are hard to solve. Alternatively, the enzyme-kinetics approach, which formulates a set of linear equations, simplifies the biological processes as transitions between discrete chemical states, but generally, it might not be accurate. Here, we show that the enzyme-kinetic approach can lead to inaccurate fluxes, even for first-order polymerization processes. To address the problem, we propose a simplified linear-decoupling approximation for steady-state probabilities of higher-order copolymer chains under biologically relevant conditions. Our findings demonstrate that the stationary speed and error rate obtained from the linear-decoupling method align closely with exact values from the Markov-chain (nonlinear) approximation. Extending the technique to higher-order processes with proofreading and internal states shows that it works equally well to describe trade-offs between speed and accuracy for DNA replication and transcription elongation. Our work underscores the proposed linear-decoupling approximation's efficacy in addressing the nonlinear behavior of the Markov-chain approach and the enzyme-kinetic approach's limitations, ensuring accurate predictions for high-fidelity biological processes.
RESUMEN
Cleavage of dinucleotides after the misincorporational pauses serves as a proofreading mechanism that increases transcriptional elongation accuracy. The accuracy is further improved by accessory proteins such as GreA and TFIIS. However, it is not clear why RNAP pauses and why cleavage-factor-assisted proofreading is necessary despite transcriptional errors in vitro being of the same order as those in downstream translation. Here, we developed a chemical-kinetic model that incorporates most relevant features of transcriptional proofreading and uncovers how the balance between speed and accuracy is achieved. We found that long pauses are essential for high accuracy, whereas cleavage-factor-stimulated proofreading optimizes speed. Moreover, in comparison to the cleavage of a single nucleotide or three nucleotides, RNAP backtracking and dinucleotide cleavage improve both speed and accuracy. Our results thereby show how the molecular mechanism and the kinetic parameters of the transcriptional process were evolutionarily optimized to achieve maximal speed and tolerable accuracy.