Integration of bio-responsive silver in 1D photonic crystals: towards the colorimetric detection of bacteria.

The colour purity and versatility of fabrication of one-dimensional photonic crystals (1D PhCs) make them ideal candidates for colorimetric sensing of a variety of analytes. For instance, the detection of bacterial contaminants in food via colorimetric sensors can be highly appealing, as most of the existing detection techniques are in general time-consuming and the read-out requires specialised personnel. Here, we present a colorimetric sensor based on hybrid plasmonic/photonic 1D crystals. We demonstrate that the modification of the silver plasmon resonance brought about by the effective silver/bacterium interaction can be translated into the visible spectral region, producing a change in the structural colour. In addition, we observe a superior colorimetric sensitivity against the Gram negative Escherichia coli compared to the Gram positive Micrococcus luteus, a result that we attribute to the more efficient electrostatic interaction and cellular adhesion between the silver surface and the Gram-negative bacteria outer membrane. This approach demonstrates that in principle an easy colorimetric detection of bacterial contaminants can be achieved through the use of bio-responsive plasmonic materials, such as silver, whose selective electrostatic interaction with bacterial cell wall is well-known and occurs without the need of chemical functionalisation.

Large scale indium tin oxide (ITO) one dimensional gratings for ultrafast signal modulation in the visible spectral region.

Indium tin oxide (ITO) is a heavily doped semiconductor with a plasmonic response in the near infrared region. When exposed to light, the distribution of conduction band electron induces a change in the real and imaginary parts of the dielectric permittivity. The coupling of the electromagnetic waves with the electrons in the conduction band of metallic nanostructures with ultrashort light pulses results in a nonlinear plasmonic response. Such optical modulation occurring on ultrafast time scales, e.g. picosecond response times, can be exploited and used to create integrated optical components with terahertz modulation speed. Here, we present a photophysical study on a one dimensional ITO grating, realized using a femtosecond micromachining process, a very industrially accessible technology. The geometries, dimensions and pitch of the various gratings analyzed are obtained by means of direct ablation in a controlled atmosphere of a homogeneous thin layer of ITO deposited on a glass substrate. The pitch has been selected in order to obtain a higher order of the photonic band gap in the visible spectral region. Femtosecond micromachining technology guarantees precision, repeatability and extreme manufacturing flexibility. By means of ultrafast pump-probe spectroscopy, we characterize both the plasmon and inter-band temporal dynamics. We observe a large optical nonlinearity of the ITO grating in the visible range, where the photonic band gap occurs, when pumped at the surface plasmon resonance in the near infrared (1500 nm) region. All together, we show the possibility of all-optical signal modulation with heavily doped semiconductors in their transparency window with a picosecond response time through the formation of ITO grating structures.

Novel beamline for attosecond transient reflection spectroscopy in a sequential two-foci geometry.

We present an innovative beamline for extreme ultraviolet (XUV)-infrared (IR) pump-probe reflection spectroscopy in solids with attosecond temporal resolution. The setup uses an actively stabilized interferometer, where attosecond pulse trains or isolated attosecond pulses are produced by high-order harmonic generation in gases. After collinear recombination, the attosecond XUV pulses and the femtosecond IR pulses are focused twice in sequence by toroidal mirrors, giving two spatially separated interaction regions. In the first region, the combination of a gas target with a time-of-flight spectrometer allows for attosecond photoelectron spectroscopy experiments. In the second focal region, an XUV reflectometer is used for attosecond transient reflection spectroscopy (ATRS) experiments. Since the two measurements can be performed simultaneously, precise pump-probe delay calibration can be achieved, thus opening the possibility for a new class of attosecond experiments on solids. Successful operation of the beamline is demonstrated by the generation and characterization of isolated attosecond pulses, the measurement of the absolute reflectivity of SiO2, and by performing simultaneous photoemission/ATRS in Ge.

Hybrid One-Dimensional Plasmonic-Photonic Crystals for Optical Detection of Bacterial Contaminants.

Photonic crystal-based biosensors hold great promise as low-cost devices for real-time monitoring of a variety of biotargets, for example, bacterial contaminants in food. Here, we report the proof-of-concept for a new colorimetric sensor of bacterial contamination, which is based on a novel hybrid plasmonic-photonic device. Our system consists of a layer of silver, a plasmonic metal exhibiting a well-known bioactivity, on top of a one-dimensional photonic crystal. We attribute the bioresponsivity to the formation of polarization charges at the Ag/bacterium interface within a sort of "bio-doping" mechanism. Interestingly, this triggers a blue shift in the photonic response. As an example, we assessed the validity of our approach by detecting one of the most hazardous contaminants, Escherichia coli. This work demonstrates that our device can be a low-cost and portable platform for the detection of common bacterial contaminants.