Modular mucopolysaccharide gelatin naturapolyceutics hydrocolloid biomatrix with cobalt nano-additives for high density vascular network assembly.

The present study demonstrates the development of polysaccharide gelatin naturapolyceutics hydrocolloidal biomatrix with cobalt nano-additives for restructuring native tissue vasculature for tissue regenerative applications. The engineered Gelatin/Aloevera mucilage polysaccharide/nanoscaled Cobalt (GAC) hydrocolloids resulted from the intermolecular interactions between the aloevera mucilage, cobalt nano-therapeutic and gelatin. GAC hydrocolloid showed enhanced thermal stability in comparison with control Gelatin/Aloevera mucilage (GA) hydrocolloid. FTIR analysis validated that the reinforcement of aloevera mucilage and cobalt nano-therapeutic did not affect the structural integrity of the gelatin molecule. 3-Dimensional sponge-like orientation of GAC hydrocolloid facilitates perfusable biomatrix for access to nutrients and gaseous exchange for high cell adhesion and proliferation. The combined therapeutic efficacy of mucilage polysaccharides, biodegradable nanoscaled cobalt and bio-polymer enhanced the pro-angiogenic capability of the hydrocolloids by stimulating Vascular Endothelial Growth Factor (VEGF) response at wounded tissue for faster healing. The experimental outcomes on in vivo angiogenesis profiling further confirmed the development of micro vessel in chick embryonic model and regeneration of blood vessels in zebra fish model. This study opens up the potential of mucilage polysaccharides in stimulating high density angiogenesis and conveys the progress of a biocompatible, biodegradable mucilaginous hydrocolloid as an effective bio-adhesive for vascular development in soft tissue regeneration.

Biomimetic nanomaterials: Development of protein coated nanoceria as a potential antioxidative nano-agent for the effective scavenging of reactive oxygen species in vitro and in zebrafish model.

Reactive oxygen species (ROS) induced oxidative stress is one of the major factors responsible for initiation of several intracellular toxic events that leads to cell death. Antioxidant enzymes defence system of the body is responsible for maintaining the oxidative balance and cellular homeostasis. Several diseases are promoted by the excessive oxidative stress caused by the impaired antioxidant defence system that leads to oxidant/antioxidant imbalance in the body. In order to restore or precise the aberrant antioxidant system, a large number of catalytic nanoparticles has been screened so far. Exceptional antioxidative activity of nanoceria made it as a potential antioxidative nano-agent for the effective scavenging of toxic ROS. In this work albumin coated nanoceria (ANC) was synthesized and further characterised by various physicochemical techniques. The antioxidant and superoxide dismutase (SOD) assay confirm that the albumin coating do not alter the antioxidant potential of ANC. The biocompatibility and protective efficacy of ANC against oxidative stress was investigated both in vitro and in vivo in human lung epithelial (L-132) cells and zebrafish embryos, respectively. The inductively coupled plasma mass spectrometry (ICP-MS), transmission electron microscopy (TEM) and field emission scanning electron microscope (FE-SEM) analysis corroborates the uptake of ANC by the cells. Furthermore, the semi-quantitative gene expression studies confirmed that the ANC successfully defend the cells against oxidative stress by preserving the antioxidant system of the cells. Thus, the current work open up a new avenue for the development of improved antioxidant nano-drug therapies.