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1.
J Exp Med ; 152(5): 1194-309, 1980 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-6159444

RESUMEN

The effect of the X-linked CBA/N genetic defect on the ability of mice to generate primary responses to thymic-dependent and thymic-independent antigens was assessed by comparing the ability of abnormal (CBA/N x DBA/2)F1 male mice and normal (DBA/2 x CBA/N)F1 male mice to generate 2,4,6-trinitrophenyl (TNP)-specific plaque-forming cell responses to TNP-keyhole limpet hemocyanin (KLH), TNP-conjugated Ficoll (TNP-Ficoll), TNP-Brucella abortus (BA), and TNP-lipopolysaccharide (LPS). The reciprocal F1 combinations used in this study differ genetically only in the origin of their X chromosome, but differ immunologically in that (CBA/N x DBA/2)F1 male mice express all the CBA/N immune abnormalities, whereas (DBA/2 x CBA/N)F1 male mice are immunologically normal. Analysis of thymic-dependent responses to TNP-KLH revealed that abnormal F1 mice were capable of generating primary responses in vivo to high doses of TNP-KLH, but failed to generate responses to suboptimal doses of TNP-KLH that were still immunogenic for normal F1 mice. Furthermore, under limiting in vitro micro-culture conditions, the abnormal F1 mice failed to generate primary thymic-dependent responses to any dose of TNP-KLH, even though under the identical conditions normal F1 mice consistently responded to a wide antigen dose range. The cellular basis of the failure of abnormal F1 mice to respond in vitro to TNP-KLH was investigated by assaying the ability of purified populations of accessory cells, T cells, and B cells from these mice to function in responses to TNP-KLH. The results of these experiments demonstrated that helper T cells and antigen-presenting accessory cells from abnormal F1 mice were competent and functioned as well as the equivalent cell populations from normal F1 mice. Instead, the failure of CBA/N mice to generate primary in vitro responses to TNP-KLH was solely the result of a defect in their B cell population such that B cells from these mice failed to be triggered by competent helper T cells and/or competent accessory cells. Similarly, the failure of abnormal F1 mice to respond either in vivo or in vitro to TNP-Ficoll was not the result of defective accessory cell presentation of TNP-Ficoll, but was the result of the failure of B cells from these mice to be activated by competent TNP-Ficoll-presenting accessory cells. In contrast to the failure of B cells from abnormal F1 mice to be activated in vitro in response to either TNP-KLH or TNP-Ficoll, B cells from abnormal F1 mice were triggered to respond to TNP-BA and TNP-LPS, antigens that did not require accessory cell presentation. The specific failure of B cells fron abnormal F1 mice to be activated in responses that required antigen-presentation by accessory cells suggested the possibility that the X-linked CBA/N genetic defect resulted in B cell populations that might be deficient in their ability to interact with antigen-presenting accessory cells...


Asunto(s)
Linfocitos B/inmunología , Epítopos/genética , Inmunidad Celular , Animales , Antígenos de Superficie/genética , Ratones , Ratones Endogámicos CBA , Bazo/inmunología , Linfocitos T/inmunología , Trinitrobencenos/inmunología
2.
J Exp Med ; 180(5): 1901-10, 1994 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-7525843

RESUMEN

Upon primary challenge with lymphocytic choriomeningitis virus (LCMV), H-2d (BALB/cByJ) mice mount a cytotoxic T lymphocyte (CTL) response to a single immunodominant domain of the viral nucleoprotein (NP) but no detectable response to the viral glycoprotein (GP). To manipulate this CTL response, the viral NP gene was expressed in the thymus and peripheral T lymphocytes using the murine Thy1.2 promoter. As a result, such Thy1.2-NP (H-2d) transgenic (tg) mice deleted their high-affinity anti-LCMV-NP CTL, but generated equal numbers of lower-affinity NP CTL. Further, they made an alternative anti-LCMV-GP CTL response that is not normally found in non-tg mice indicating a hierarchial control of the CTL response. Unlike the H-2d mice, H-2b (C57Bl/6J) mice normally mount a CTL response to both LCMV-GP and -NP. When the LCMV-NP was expressed using the Thy1.2 promoter in these H-2b mice, the LCMV-NP-specific CTL response was completely aborted and no CTL to new, alternative viral epitopes were generated. Dilutions of H-2b or H-2d NP peptides indicated that 3-4 logs less H-2b NP peptide was required to sensitize syngeneic target cells for CTL-specific lysis, suggesting that the differing affinities of H-2b and H-2d major histocompatibility complex molecules for their peptides likely account for the total removal of NP CTL in the H-2b mice but only partial removal in H-2d mice made to express thymic NP. Thymic grafting experiments done with thymi from newborn Thy1.2-NP tg mice show that selection processes studied in this model are of central (thymic) origin and are not caused by Thy1.2-positive LCMV-NP-expressing T lymphocytes in the periphery.


Asunto(s)
Virus de la Coriomeningitis Linfocítica/inmunología , Linfocitos T Citotóxicos/inmunología , Timo/fisiología , Proteínas Virales/inmunología , Adaptación Fisiológica , Animales , Secuencia de Bases , Epítopos , Femenino , Glicoproteínas/biosíntesis , Glicoproteínas/genética , Glicoproteínas/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Transgénicos , Datos de Secuencia Molecular , Nucleoproteínas/biosíntesis , Nucleoproteínas/genética , Nucleoproteínas/inmunología , Proteínas Virales/biosíntesis , Proteínas Virales/genética
3.
Science ; 237(4820): 1324-9, 1987 Sep 11.
Artículo en Inglés | MEDLINE | ID: mdl-2888190

RESUMEN

Human T-lymphotropic virus type 1 (HTLV-1) is a suspected causative agent of adult T-cell leukemia. One of the viral genes encodes a protein (tat) that not only results in transactivation of viral gene expression but may also regulate the expression of certain cellular genes that are important for cell growth. Transgenic mice that expressed the authentic tat protein under the control of the HTLV-1 long terminal repeat were generated, and cell types that are permissive for the viral promoter and the effects of the tat gene on these cells were studied. Three of eight founder mice with high levels of expression of the transgene in muscle were bred and then analyzed. All developed soft tissue tumors at multiple sites between 13 to 17 weeks of age. This phenotype was transmitted to nine of nine offspring that inherited the tat gene and were available for analysis. The remaining five founders expressed the transgene in the thymus, as well as in muscle. This second group of mice all exhibited extensive thymic depletion and growth retardation; in all of these mice, death occurred between 3 to 6 weeks of age before tumors became macroscopically visible. The tat gene under the control of the HTLV-1 regulatory region showed tissue-specific expression and the tat protein efficiently induced mesenchymal tumors. The data establish tat as an oncogenic protein and HTLV-1 as a transforming virus.


Asunto(s)
Infecciones por Deltaretrovirus/genética , Deltaretrovirus/genética , Genes Virales , Mesenquimoma/microbiología , Animales , Secuencia de Bases , Femenino , Ingeniería Genética , Vectores Genéticos , Masculino , Mesenquimoma/genética , Ratones , Linaje , Plásmidos , Biosíntesis de Proteínas , Transcripción Genética
4.
Science ; 237(4820): 1340-3, 1987 Sep 11.
Artículo en Inglés | MEDLINE | ID: mdl-2888191

RESUMEN

Human T-lymphotropic virus type 1 (HTLV-1) has been associated with the neurologic disorder tropical spastic paraparesis and possibly with multiple sclerosis. The tat gene of HTLV-1 under control of its own long terminal repeat is capable of inducing tumors in transgenic mice. The morphologic and biologic properties of these tumors indicate their close resemblance to human neurofibromatosis (von Recklinghausen's disease), the most common single gene disorder to affect the nervous system. The high spontaneous incidence of this disease, together with the diverse clinical and pathologic features associated with it, suggests that environmental factors may account for some of the observed cases. Multiple tumors developed simultaneously in the transgenic tat mice at approximately 3 months of age, and the phenotype was successfully passed through three generations. The tumors arise from the nerve sheaths of peripheral nerves and are composed of perineural cells and fibroblasts. Tumor cells from these mice adapt easily to propagation in culture and continue to express the tat protein in significant amounts. When transplanted into nude mice, these cultured cells efficiently induce tumors. Evidence of HTLV-1 infection in patients with neural and other soft tissue tumors is needed in order to establish a link between infection by this human retrovirus and von Recklinghausen's disease and other nonlymphoid tumors.


Asunto(s)
Infecciones por Deltaretrovirus/genética , Neurofibromatosis 1/genética , Animales , Infecciones por Deltaretrovirus/patología , Modelos Animales de Enfermedad , Técnica del Anticuerpo Fluorescente , Ingeniería Genética , Humanos , Ratones , Ratones Desnudos , Neurofibromatosis 1/microbiología , Neurofibromatosis 1/patología , Proteínas Virales de Fusión/análisis
5.
Science ; 258(5089): 1792-5, 1992 Dec 11.
Artículo en Inglés | MEDLINE | ID: mdl-1299224

RESUMEN

Mice transgenic for the human T cell leukemia virus (HTLV-I) Tax gene develop fibroblastic tumors that express NF-kappa B-inducible early genes. In vitro inhibition of NF-kappa B expression by antisense oligodeoxynucleotides (ODNs) inhibited growth of these culture-adapted Tax-transformed fibroblasts as well as an HTLV-I-transformed human lymphocyte line. In contrast, antisense inhibition of Tax itself had no apparent effect on cell growth. Mice treated with antisense to NF-kappa B ODNs showed rapid regression of transplanted fibrosarcomas. This suggests that NF-kappa B expression may be necessary for the maintenance of the malignant phenotype and provides a therapeutic approach for HTLV-I-associated disease.


Asunto(s)
Fibrosarcoma/tratamiento farmacológico , Genes pX , Virus Linfotrópico T Tipo 1 Humano/genética , FN-kappa B/antagonistas & inhibidores , Oligonucleótidos Antisentido/farmacología , Oligonucleótidos Antisentido/uso terapéutico , Células 3T3 , Animales , Secuencia de Bases , División Celular/efectos de los fármacos , Línea Celular , Transformación Celular Neoplásica , Fibrosarcoma/patología , Humanos , Cinética , Linfocitos , Ratones , Ratones Transgénicos , Datos de Secuencia Molecular , FN-kappa B/genética , Oligodesoxirribonucleótidos
6.
Neuron ; 5(2): 187-97, 1990 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-2116814

RESUMEN

To gain insights into transcription factors defining neuronal identity, we generated transgenic mice carrying a 1.8 kb rat neuron-specific enolase (NSE) promoter fragment fused to an E. coli lacZ gene. Four of seven transgenic families expressed transgene RNA in the nervous system but not in most other tissues. Histochemical analysis of adult brain from the two lines with highest lacZ mRNA levels showed neuron-specific, pan-neuronal beta-galactosidase activity. Developmental RNA and histochemical analyses showed parallel onset of transgene and endogenous NSE gene expression in various neuronal cell types, although the magnitude of NSE mRNA accumulation later in development was not matched by the transgene. These results suggest that cis-acting regulatory elements, subject to neuron-specific control, are located within 1.8 kb upstream from the NSE gene.


Asunto(s)
Regulación de la Expresión Génica , Neuronas/enzimología , Fosfopiruvato Hidratasa/genética , Regiones Promotoras Genéticas , Animales , Secuencia de Bases , Northern Blotting , Southern Blotting , Encéfalo/metabolismo , Clonación Molecular , Genes , Operón Lac , Masculino , Ratones , Ratones Transgénicos , Datos de Secuencia Molecular , Secuencias Reguladoras de Ácidos Nucleicos , Testículo/metabolismo , beta-Galactosidasa/genética , beta-Galactosidasa/metabolismo
7.
J Clin Invest ; 96(3): 1548-55, 1995 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-7657825

RESUMEN

HTLV-1 infection causes an adult T cell leukemia in humans. The viral encoded protein tax, is thought to play an important role in oncogenesis. Our previous data obtained from a tax transgenic mouse model revealed that tax transforms mouse fibroblasts but not thymocytes, despite comparable levels of tax expression in both tissues. Constitutive tyrosine phosphorylation of a 130-kD protein(s) was observed in the tax transformed fibroblast B line and in HTLV-1 transformed human lymphoid lines, but not in thymocytes from Thy-tax transgenic mice. Phosphotyrosine immunoprecipitation followed by Western blot analysis with a set of Jak kinase specific antibodies, identified p130 as Jak2 in the tax transformed mouse fibroblastic cell line and Jak3 in HTLV-1 transformed human T cell lines. Phosphorylation of Jak2 in tax transformed cells resulted from high expression of IL-6. Tyrosine phosphorylation of this protein could also be induced in Balb/c3T3 cells using a supernatant from the B line, which was associated with induction of cell proliferation. Both phosphorylation and proliferation were inhibited by IL-6 neutralizing antibodies. Constitutive phosphorylation of Jak kinases may facilitate tumor growth in both HTLV-1 infected human T cells and the transgenic mouse model.


Asunto(s)
Transformación Celular Viral , Productos del Gen tax/biosíntesis , Virus Linfotrópico T Tipo 1 Humano/genética , Proteínas Tirosina Quinasas/metabolismo , Proteínas Proto-Oncogénicas , Células 3T3 , Animales , Secuencia de Bases , División Celular/efectos de los fármacos , Línea Celular , Cartilla de ADN , Activación Enzimática , Expresión Génica , Virus Linfotrópico T Tipo 1 Humano/metabolismo , Humanos , Interleucina-6/biosíntesis , Interleucina-6/farmacología , Janus Quinasa 2 , Janus Quinasa 3 , Cinética , Ratones , Ratones Transgénicos , Datos de Secuencia Molecular , FN-kappa B/metabolismo , Sondas de Oligonucleótidos , Reacción en Cadena de la Polimerasa , Receptores de Interleucina/biosíntesis , Receptores de Interleucina-6 , Linfocitos T , Transfección , Células Tumorales Cultivadas
8.
Mol Cell Biol ; 14(8): 5371-83, 1994 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-8035815

RESUMEN

To analyze regulation of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR), cell lines were generated from LTR-tax x LTR-beta-galactosidase (beta-Gal) doubly transgenic mouse fibroblastic tumors. The HTLV-I LTR directs expression of both the tax and lacZ genes, and Tax up-modulates both promoters in primary cells. However, once cells were transformed by tax, beta-Gal but not tax expression was suppressed. Supertransformation of these cells with v-src suppressed both beta-Gal and tax expression. This suppression was reversed by treatment with the tyrosine kinase inhibitor herbimycin A or protein kinase A inhibitor H8. Electrophoretic mobility shift assays demonstrated augmented binding in the R but not U3 region. This binding was competitively inhibited by a high-affinity CREB oligodeoxynucleotide and super-shifted with a specific CREB antibody. Treatment of cells with the cyclic AMP analog dibutyryl cyclic AMP also transiently increased the R region binding dramatically. In vitro DNase I footprint analysis identified a protein-binding sequence in the R region which corresponded with suppression. However, this target sequence lacked a conventional CREB-binding site. A 70.5-kDa DNA-binding protein was partially purified by affinity chromatography, along with a 49-kDa protein which reacted with CREB-specific sera. These data demonstrate that HTLV-I LTR suppression is associated with CREB factor binding in the R region, probably by direct interaction with a 70.5-kDa protein, and provide a novel mechanism for maintenance of viral latency.


Asunto(s)
Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Regulación Viral de la Expresión Génica , Virus Linfotrópico T Tipo 1 Humano/genética , Secuencias Repetitivas de Ácidos Nucleicos , Animales , Secuencia de Bases , Sitios de Unión , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Proteínas de Unión al ADN/metabolismo , Genes pX , Genes src , Ratones , Ratones Transgénicos , Datos de Secuencia Molecular , Proteínas Nucleares/metabolismo , ARN Mensajero/genética , Transcripción Genética
9.
Nat Biotechnol ; 18(2): 199-204, 2000 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-10657128

RESUMEN

We have developed a method for anchored amplification on a microchip array that allows amplification and detection of multiple targets in an open format. Electronic anchoring of sets of amplification primers in distinct areas on the microchip permitted primer-primer interactions to be reduced and distinct zones of amplification created, thereby increasing the efficiency of the multiplex amplification reactions. We found strand displacement amplification (SDA) to be ideal for use in our microelectronic chip system because of the isothermal nature of the assay, which provides a rapid amplification system readily compatible with simple instrumentation. Anchored SDA supported multiplex DNA or RNA amplification without decreases in amplification efficiency. This microelectronic chip-based amplification system allows multiplexed amplification and detection to be performed on the same platform, streamlining development of any nucleic acid-based assay.


Asunto(s)
Electrónica/métodos , Proteínas de la Membrana , Técnicas de Amplificación de Ácido Nucleico , Hibridación de Ácido Nucleico/métodos , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Aromatasa/genética , Proteínas Bacterianas/genética , Chlamydia trachomatis/genética , Cartilla de ADN , Factor V/genética , Antígenos HLA/genética , Proteína de la Hemocromatosis , Antígenos de Histocompatibilidad Clase I/genética , Humanos
10.
Nucleic Acids Res ; 28(7): E17, 2000 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-10710434

RESUMEN

We describe a method for the discrimination of short tandem repeat (STR) alleles based on active microarray hybridization. An essential factor in this method is electronic hybridization of the target DNA, at high stringency, in <5 min. High stringency is critical to avoid slippage of hybrids along repeat tracts at allele-specific test sites in the array. These conditions are attainable only with hybridization kinetics realized by electronic concentration of DNA. A sandwich hybrid is assembled, in which proper base stacking of juxtaposed terminal nucleotides results in a thermodynamically favored complex. The increased stability of this complex relative to non-stacked termini and/or base pair mismatches is used to determine the identification of STR alleles. This method is capable of simultaneous and precise identification of alleles containing different numbers of repeats, as well as mutations within these repeats. Given the throughput capabilities of microarrays our system has the potential to enhance the use of microsatellites in forensic criminology, diagnostics and genetic mapping.


Asunto(s)
ADN/análisis , Hibridación de Ácido Nucleico/métodos , Análisis de Secuencia por Matrices de Oligonucleótidos , Secuencias Repetidas en Tándem/genética , Alelos , ADN/química , Electroquímica , Estudios de Evaluación como Asunto , Variación Genética , Humanos , Yoduro Peroxidasa/genética , Receptor de Factor Estimulante de Colonias de Macrófagos/genética , Tirosina 3-Monooxigenasa/genética
11.
Oncogene ; 13(1): 135-42, 1996 Jul 04.
Artículo en Inglés | MEDLINE | ID: mdl-8700539

RESUMEN

Human T cell leukemia virus type I (HTLV-1) is the etiologic agent of adult T-cell leukemia (ATL) and HTLV-1 associated myelopathy, also called tropical spastic paraparesis (HAM/TSP). Both clinical and in vitro evidence have demonstrated that the virus or its transactivator Tax, are transforming. However, transformation appears to require additional, as yet poorly characterized, genetic changes in infected cells. JNK is a recently characterized member of the MAP kinase family. Its signaling cascade is distinct from other members and has been demonstrated to play an important role in T-cell activation, at least partially through its downstream targets, c-jun and ATF-2. Here we demonstrate constitutive activation of the JNK cascade in human lymphocytes transformed in vitro by HTLV-1 and also in Tax transformed murine fibroblasts. Such activation is not induced by Tax expression alone, and occurs only when infected lymphocytes become IL-2 independent or immortalized. Constitutive JNK activation was also found in leukocytes isolated from ATL patients. The acquisition of constitutive JNK activation may represent an important later event in HTLV-1 tumorigenesis.


Asunto(s)
Transformación Celular Neoplásica , Transformación Celular Viral , Virus Linfotrópico T Tipo 1 Humano/patogenicidad , Proteínas Quinasas JNK Activadas por Mitógenos , Leucemia-Linfoma de Células T del Adulto/etiología , Quinasas de Proteína Quinasa Activadas por Mitógenos , Proteínas Quinasas/metabolismo , Animales , Línea Celular Transformada , Activación Enzimática , Fibroblastos/enzimología , Fibroblastos/patología , Fibroblastos/virología , Productos del Gen tax/fisiología , Humanos , Leucemia-Linfoma de Células T del Adulto/patología , Linfocitos/enzimología , Linfocitos/patología , Linfocitos/virología , MAP Quinasa Quinasa 4 , Ratones , Ratones Endogámicos BALB C , Ratones Transgénicos , Proteínas de Neoplasias/metabolismo , Transducción de Señal , Células Tumorales Cultivadas
12.
Hum Gene Ther ; 7(4): 471-7, 1996 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-8800741

RESUMEN

Human T cell leukemia virus type 1 (HTLV-1) causes adult T cell leukemia (ATL), and the virus-encoded trans-activator, Tax, plays an important role in T cell transformation. In the HTLV-1 long terminal repeat (LTR)-Tax transgenic mouse model, Tax expression causes fibroblastic tumors. A tumor-derived cell line (B line) obtained from an explant of a Tax-transformed tumor, was established. This line expresses high levels of many cytokines as a consequence of Tax activation. However, the tumors are not immunogenic when transplanted into syngeneic mice. Because B line cells do not express the immunogenic cytokine interferon-gamma (IFN-gamma), a replication-defective adenoviral vector was used to deliver the IFN-gamma gene to tumor cells. The recombinant IFN-gamma adenovirus (IFN-gamma/Ad) can efficiently infect B line cells, resulting in high levels of IFN-gamma expression and secretion. Local secretion of IFN-gamma from B line cells caused both CD(4+)- and CD(8+)-positive T cell infiltration, and completely inhibited local tumor development in transplanted mice. Immunization with these cells significantly delayed tumor development after subsequent challenges of parental tumor cells. Expression of IFN-gamma in B cells also partially inhibited the highly expressed immune suppressive cytokine, transforming growth factor-beta 1 (TGF-beta 1). This system provides us with a valuable tumor immune therapy model to evaluate the effects of cytokines in induction or inhibition of specific antitumor immunity.


Asunto(s)
Adenovirus Humanos/genética , Fibrosarcoma/terapia , Terapia Genética , Virus Linfotrópico T Tipo 1 Humano , Interferón gamma/genética , Animales , Linfocitos B/citología , Regulación hacia Abajo , Expresión Génica , Productos del Gen tax/genética , Productos del Gen tax/metabolismo , Técnicas de Transferencia de Gen , Vectores Genéticos , Humanos , Interferón gamma/metabolismo , Interferón gamma/uso terapéutico , Ratones , Ratones Endogámicos C57BL , Trasplante de Neoplasias , Factor de Crecimiento Transformador beta/genética , Factor de Crecimiento Transformador beta/metabolismo , Células Tumorales Cultivadas , Vacunación
13.
Neurology ; 41(10): 1687-9, 1991 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-1922822

RESUMEN

An HTLV-I tax transgenic mouse model develops a syndrome with similarities to type 1 neurofibromatosis (NF-1). To investigate possible associations between this human retrovirus and NF-1, we have analyzed 67 neurofibromas from Japan (where HTLV-I infection is endemic) and compared them with 21 cases from the United States. We were not able to identify virus in tumor tissue in either group. This suggests that HTLV-I infection is not commonly associated with NF-1.


Asunto(s)
Infecciones por HTLV-I/complicaciones , Neurofibromatosis 1/complicaciones , Secuencia de Aminoácidos , ADN Viral/análisis , Infecciones por HTLV-I/epidemiología , Infecciones por HTLV-I/genética , Humanos , Japón/epidemiología , Datos de Secuencia Molecular , Neurofibromatosis 1/epidemiología , Neurofibromatosis 1/genética , Reacción en Cadena de la Polimerasa , Estados Unidos/epidemiología
14.
J Neuroimmunol ; 52(1): 61-8, 1994 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-8207120

RESUMEN

Astrocytes provide crucial support for neurons and their impairment by viruses or their interactions with anti-viral or autoimmune responses could contribute to neurological disease. We have developed a transgenic mouse model to assess lymphocyte-astrocyte interactions. The major histocompatibility complex (MHC) class I molecule, Db, was expressed in astrocytes under the transcriptional control of regulatory sequences from the glial fibrillary acidic protein (GFAP) gene. Baseline cerebral MHC class I mRNA levels from transgenic mice were elevated over those of non-transgenic controls, and a prominent increase in cerebral MHC class I expression occurred following focal, injury-induced astroglial activation within transgenic brains but not in non-transgenic controls. FACS analysis of explant astrocyte cultures from established transgenic lines demonstrated astroglial expression of the GFAP-Db fusion gene at the protein level. Functional antigen-presenting capacity was conferred by the Db transgene, as virus-infected primary astrocytes obtained from transgenic BALB/c mice (KdIdDdLd) expressing the Db molecule were lysed by Db-restricted anti-viral CTL.


Asunto(s)
Astrocitos/inmunología , Proteína Ácida Fibrilar de la Glía/genética , Antígenos H-2/biosíntesis , Virus de la Coriomeningitis Linfocítica/inmunología , Linfocitos T Citotóxicos/inmunología , Animales , Secuencia de Bases , Femenino , Proteína Ácida Fibrilar de la Glía/análisis , Antígenos H-2/genética , Antígeno de Histocompatibilidad H-2D , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Transgénicos , Datos de Secuencia Molecular , ARN Mensajero/análisis
15.
AIDS Res Hum Retroviruses ; 12(9): 829-32, 1996 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-8738435

RESUMEN

Genomic footprinting of integrated HTLV-I and HIV-1 confirmed many aspects of retroviral transcriptional regulation deduced from previous studies. However, many notable differences were seen. HTLV-I genomic protein-binding patterns corresponded more closely to elements defined by transient transfection expression studies than to those mapped by in vitro protein-binding studies. HIV-1 genomic footprinting showed activation-related binding to adjacent NF-KB/SP1 sites and a large (90 bp) region transversing the R/U5 boundary, but minimal protein binding to NFAT, NRE, LBP-1, and CTF/NF1 sites relative to previous in vitro footprinting studies.


Asunto(s)
Huella de ADN , VIH-1/genética , Virus Linfotrópico T Tipo 1 Humano/genética , Linfocitos T/virología , Secuencia de Bases , Línea Celular , Humanos , Datos de Secuencia Molecular , Unión Proteica , Provirus/genética , Secuencias Reguladoras de Ácidos Nucleicos , Acetato de Tetradecanoilforbol/farmacología , Factores de Transcripción/metabolismo , Transcripción Genética , Transfección , Integración Viral
17.
J Neurol Sci ; 106(1): 60-6, 1991 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-1779240

RESUMEN

We describe here a 10-year-old patient with high levels of serum IgE and inflammatory myopathy whose muscle fibers exhibit excessive autophagy. Previous studies have demonstrated surface expression of class I MHC antigens on muscle fibers from patients with inflammatory myopathy. The muscle fibers of this patients showed marked expression of both class I and class II MHC antigens. The reaction products were demonstrated not only on sarcolemma but also in and around some vacuoles. Both CD4-positive and CD8-positive T-lymphocytes were noted in inflammatory exudates surrounding these fibers but B-lymphocytes were rare. We hypothesize that myocyte expression of both class I and class II antigens may play a role in the pathogenesis of this new type of inflammatory myopathy.


Asunto(s)
Enfermedades Autoinmunes/inmunología , Antígenos HLA/análisis , Antígenos HLA-DR/análisis , Músculos/inmunología , Miositis/inmunología , Enfermedades Autoinmunes/tratamiento farmacológico , Enfermedades Autoinmunes/patología , Niño , Humanos , Inmunoglobulina E/sangre , Cuerpos de Inclusión Viral , Masculino , Músculos/patología , Miositis/tratamiento farmacológico , Miositis/patología , Fagocitosis , Prednisolona/uso terapéutico , Vacuolas
18.
J Investig Med ; 48(2): 93-101, 2000 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-10736968

RESUMEN

BACKGROUND: A simple yet powerful tool for providing for rapid gene identification in the clinic would be the combination of isothermal gene amplification with electronic microchip analysis. This is a first report of such a union of these technologies. METHODS: The first assay demonstrates discrimination between four bacterial pathogens. For this, one portion of the bacterial 16S rRNA gene encompassing a microheterogeneous region was isothermally amplified using Strand Displacement Amplification (SDA). Type identification was then made by "sandwich" assay format either using selective electronic hybridization of amplicons to sequence-specific capture oligonucleotides and a universal, fluorescently labeled reporter oligonucleotide, or, alternatively, sequence-specific reporters and a universal capture oligonucleotide. The second assay tested for the presence or absence of the Factor V Leiden point mutation using DNA obtained from 18 patients in a blind assay. For this, allele-specific SDA was developed. Following amplification using a sense-biotinylated primer and either the corresponding antisense wild type or mutant primer, multiple patient amplicons were targeted to specified locations on the microarray and visualized using a fluorescently labeled reporter oligonucleotide. Positive signals were scored as greater than or equal to two times the background. RESULTS: Bacterial type-specific signals were between 3- to 10-fold greater than nonspecific in both assay formats. Using allele-specific SDA, 100% agreement was observed between PAGE analysis, microarray results, and clinical diagnosis in Factor V mutation analysis. CONCLUSIONS: We demonstrated two model clinical assays combining amplified materials and microelectronic arrays, one potentially suitable for pathogen screening and the other for a deleterious genetic mutation.


Asunto(s)
Bacterias/genética , Bacterias/aislamiento & purificación , Análisis Mutacional de ADN/métodos , Bacterias/patogenicidad , Secuencia de Bases , Cartilla de ADN/genética , Electrónica Médica , Factor V/genética , Amplificación de Genes , Genes Bacterianos , Pruebas Genéticas , Humanos , Análisis de Secuencia por Matrices de Oligonucleótidos , ARN Bacteriano/genética , ARN Ribosómico 16S/genética
19.
Inflammation ; 23(6): 583-90, 1999 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-10565571

RESUMEN

Oligonucleotide N3'->P5' Phosphoramidates (PN) may confer advantages over unmodified phosphodiester compounds for therapeutic applications (1). Previous in vitro data demonstrated that PN Oligodeoxynucleotides (ODNs) possess several advantageous features, including RNase H-independence, an improved resistance to nuclease degradation, decreased protein binding, and high affinity sequence-specific binding to complementary RNAs (1, 2). Consequently, we undertook a study to investigate the effects of PN antisense (AS) oligos targeted against the p65 subunit of the Nuclear Factor Kappa beta (NF-kappaB) transcription factor in vivo, in mice. The ability of the antisense molecules to inhibit IL-6 elevation induced by lipopolysaccharide (LPS) in mice, was studied. A 16 mer uniformly modified PN and a chimeric phosphoramidate-phosphodiester oligodeoxynucleotide complementary to the region surrounding the starting codon, (PN-PO-PN) of the NK-kappaB p65 subunit mRNA, both caused a sequence specific reduction of the serum IL-6 level in mice. A scrambled oligodeoxynucleotide showed much lower IL-6 inhibition in mice. These results show that the p65 PN-AS can modulate expression of IL-6 in mice without uptake enhancers and therefore may be a useful prototype for RNAse-H independent therapeutic agents.


Asunto(s)
Amidas/antagonistas & inhibidores , Regulación de la Expresión Génica , Interleucina-6/biosíntesis , FN-kappa B/genética , Oligonucleótidos Antisentido/genética , Oligonucleótidos Antisentido/farmacología , Ácidos Fosfóricos/antagonistas & inhibidores , Animales , Regulación de la Expresión Génica/efectos de los fármacos , Interleucina-6/antagonistas & inhibidores , Interleucina-6/genética , Ratones , FN-kappa B/antagonistas & inhibidores
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