Physicochemical properties and in vitro digestive behavior of astaxanthin loaded Pickering emulsion gel regulated by konjac glucomannan and κ-carrageenan.

This study aimed to elaborate the combination effect of polysaccharides on physicochemical properties and in vitro digestive behavior of astaxanthin (AST)-loaded Pickering emulsion gel. AST-loaded Pickering emulsion gel was prepared by heating Pickering emulsion with konjac glucomannan (KGM) and κ-carrageenan (CRG). The microstructure revealed that adding the two polysaccharides resulted in Pickering emulsion forming a network structure. It exhibited a denser and more uniform network structure, enhancing its mechanical properties four times and increasing its water-holding capacity by 20 %. In vitro digestion experiments demonstrated that the release of free fatty acids from the Pickering emulsion gel (4.25 %) was notably lower than that from conventional Pickering emulsion (17.19 %), whereas AST bioaccessibility was remarkably low at 0.003 %. It provided a feasible strategy to regulate the bioaccessibility in Pickering emulsion, which has theoretical significance to guide the current eutrophic diet people.

Rheological and physicochemical properties of heat-induced ovalbumin gels in presence of sodium carboxymethyl cellulose.

In this study, the effect of sodium carboxymethylcellulose (CMC-Na) on the rheological and physicochemical properties of heat-induced ovalbumin (OVA) gels was evaluated. The OVA/CMC-Na composite gels were prepared by heat-induced (85 °C, pH 7.0) a mixture of CMC-Na (0, 0.2, 0.4, 0.8 and 1%) and OVA. The results revealed that the addition of CMC-Na dramatically reduced the springiness and hardness of the composite gels, while slightly enhancing the intermolecular hydrogen bonding interactions, which facilitated the improvement of the softness of the gels. It can be observed by SEM that the added CMC-Na was stacked on the surface of the OVA, resulting in visible "linear bumps". All gel samples exhibited shear-thinning behavior. The apparent viscosity of the composite gels increased with the addition of CMC-Na, and the OVA gel with 1% CMC-Na showed the highest apparent viscosity and the lowest storage modulus (G'). Additionally, low field nuclear magnetic resonance (LF-NMR) measurements indicated that the increasing CMC-Na boosted the water mobility of the composite gel. This study offers a novel approach to the development of ovalbumin-based soft gel foods, especially for certain populations with swallowing difficulties.

Construction of astaxanthin loaded Pickering emulsions gel stabilized by xanthan gum/lysozyme nanoparticles with konjac glucomannan from structure, protection and gastrointestinal digestion perspective.

Pickering emulsion gels have demonstrated their efficacy in delivering bioactive compounds by effectively preventing droplet aggregation, Ostwald maturation, and phase separation through gel network. Astaxanthin (AST) Pickering emulsion gels stabilized by xanthan gum/lysozyme nanoparticles (XG/Ly NPs) and konjac glucomannan (KGM) were studied from rheological tests and textural analysis. The Pickering emulsion gel demonstrated the highest water holding capacity (WHC) at concentration of 2 % XG/Ly NPs, 60 % oil phase fraction, and 0.5 % KGM concentration. The presence of KGM was observed to enhance the plasticity of Pickering emulsion gels, as evidenced by the dense gel network structure formed on the surface of the droplets. Furthermore, the utilization of Pickering emulsion gels containing AST has demonstrated enhanced photostability and a protective effect on AST, as evidenced by antioxidant experiments. Moreover, the incorporation of KGM in Pickering emulsion gels has been found to reduce the release of free fatty acids (FFA) and the bioaccessibility of AST, as indicated in vitro digestion results. Overall, these findings indicate the potential of KGM-based Pickering emulsion gels as effective vehicles for the delivery of hydrophobic bioactive compounds within the food industry.

Pickering emulsions synergistic stabilized with konjac glucomannan and xanthan gum/lysozyme nanoparticles: Structure, protection and gastrointestinal digestion.

The effect of konjac glucomannan (KGM) on the stability and digestion characteristics of xanthan gum/lysozyme nanoparticles (XG/Ly NPs) stabilized Pickering emulsions was investigated. Results indicated that the high viscosity of KGM prompted the particles to be adsorbed toward the interface, which decreased the particle size and increased the stability of emulsions. As the concentration of KGM increased, the G' and G″ of emulsions became larger and approached a "solid-like" state. When the KGM concentration was ≥0.2 %, the large amplitude sweeps of the emulsion exhibited a "weak strain overshoot". The network structure formed by KGM molecular chain and particles was intertwined around the droplets to form a polysaccharide layer and fibrous network structure. Emulsions containing KGM showed a "spider web" epidermal network pattern. It was found by illumination for 4 h that samples with 0.2 % KGM concentration increased the retention of ß-carotene by 18.74 %. KGM decreased the release rate of fatty acids and bioaccessibility by hindering bile salt and lipase adsorption.

Reversibility of freeze-thaw/re-emulsification on Pickering emulsion stabilized with gliadin/sodium caseinate nanoparticles and konjac glucomannan.

The reversibility of freeze-thaw/re-emulsification of Pickering emulsion stabilized by gliadin/sodium caseinate nanoparticles (Gli/CAS NPs) was improved by adding konjac glucomannan (KGM). With the increase in the KGM concentration, the delamination of emulsions after freeze-thaw treatment was significantly improved. The microstructure showed that the presence of KGM helped to maintain the network structure of continuous phases. In particular, the particle size of the emulsion did not increase significantly after three freeze-thaw cycles when the KGM concentration was 0.6 % and the oil phase fraction was 60 %. The results of flocculation degree and coalescence degree also indicated that KGM promoted the cross-linking between particles on the surface of the droplet and increased the thickness of the interfacial film of the droplet. Rheological analysis also proved the same result: the elastic modulus of the emulsion was still larger than the viscous modulus, which showed the ideal freeze thaw reversibility. After adding KGM, the emulsion formed a strong network structure with good stability for long-term storage and reversibility for freeze-thaw cycling/re-emulsification. Thus, the emulsion has broad application prospects in food, cosmetics, and pharmaceutical fields.

Insight into the interaction between tannin acid and bovine serum albumin from a spectroscopic and molecular docking perspective.

In this study, the interaction mechanism of bovine serum albumin (BSA) with tannic acid (TA) was investigated by spectroscopic and computational approaches and further validated using circular dichroism (CD), differential scanning calorimetry (DSC) and molecular docking techniques. The fluorescence spectra showed that TA bound to BSA and underwent static quenching at a single binding site, which was consistent with the molecular docking results. And the fluorescence quenching of BSA by TA was dose-dependent. Thermodynamic analysis indicated that hydrophobic forces dominated the interaction of BSA with TA. The results of circular dichroism showed that the secondary structure of BSA was slightly changed after coupling with TA. Differential scanning calorimetry showed that the interaction between BSA and TA improved the stability of the BSA-TA complex, and the melting temperature increased to 86.67 °C and the enthalpy increased to 264.1 J g-1 when the ratio of TA to BSA was 1.2 : 1. Molecular docking techniques revealed specific amino acid binding sites for the BSA-TA complex with a docking energy of -12.9 kcal mol-1, which means the TA is non-covalently bound to the BSA active site.

Comprehensive Overview of Carboxamide Derivatives as Succinate Dehydrogenase Inhibitors.

Up to now, a total of 24 succinate dehydrogenase inhibitors (SDHIs) fungicides have been commercialized, and SDHIs fungicides were also one of the most active fungicides developed in recent years. Carboxamide derivatives represented an important class of SDHIs with broad spectrum of antifungal activities. In this review, the development of carboxamide derivatives as SDHIs with great significances were summarized. In addition, the structure-activity relationships (SARs) of antifungal activities of carboxamide derivatives as SDHIs was also summarized based on the analysis of the structures of the commercial SDHIs and lead compounds. Moreover, the cause of resistance of SDHIs and some solutions were also introduced. Finally, the development trend of SDHIs fungicides was prospected. We hope this review will give a guide for the development of novel SDHIs fungicides in the future.

Comprehensive Overview of ß-Methoxyacrylate Derivatives as Cytochrome bc1 Inhibitors for Novel Pesticide Discovery.

ß-Methoxyacrylate derivatives represent a new class of pesticides, which have attracted increasing attention owing to their unique structure, broad biological activity, and unique mechanisms of action. They inhibit mitochondrial respiration via preventing electron transfer at the Qo site of the cytochrome bc1 complex and thus are identified as cyt bc1 inhibitors. A variety of ß-methoxyacrylate derivatives have been reported by many research groups for discovery of novel pesticides with improved expected activities. This review focuses on development of ß-methoxyacrylate derivatives with great significance as pesticides such as fungicides, acaricides, insecticides, herbicides, and antiviral agents. In addition, the structure-activity relationships (SARs) of ß-methoxyacrylate derivatives are summarized. Moreover, the cause of resistance to ß-methoxyacrylate fungicides and some solutions are also introduced. Finally, the development trend of ß-methoxyacrylate derivatives as pesticides is explored. We hope the review will give a guide to develop novel ß-methoxyacrylate pesticides in the future.

Characterization and antibacterial behavior of an edible konjac glucomannan/soluble black tea powder hybrid film with ultraviolet absorption.

In this study, a KGM/SBTP film was prepared by a blending method using KGM and a soluble black tea film (SBTP) as substrates, and its hygroscopicity, thermal properties, light barrier properties, microstructure, and bacteriostatic properties were evaluated. The results confirmed that compared with the control group, with the increase in the SBTP content, the transmittance of the film in the ultraviolet region significantly reduced, and the water barrier property and thermal stability were improved. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) results indicated that the tea polyphenols interacted with the film substrate. SEM also showed that the structure of the KGM/SBTP films was smooth and flat, and all samples showed no fracture. In addition, the KGM/SBTP mixed membrane had obvious concentration-dependent antibacterial activity. When the concentration of SBTP was 0.9%, the inhibition zones against Staphylococcus aureus and Escherichia coli were 12.30 ± 0.20 mm and 12.05 ± 0.47 mm, respectively.