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1.
Proc Natl Acad Sci U S A ; 111(10): 3865-70, 2014 Mar 11.
Artículo en Inglés | MEDLINE | ID: mdl-24613931

RESUMEN

Bitopic integral membrane proteins with a single transmembrane helix play diverse roles in catalysis, cell signaling, and morphogenesis. Complete monospanning protein structures are needed to show how interaction between the transmembrane helix and catalytic domain might influence association with the membrane and function. We report crystal structures of full-length Saccharomyces cerevisiae lanosterol 14α-demethylase, a membrane monospanning cytochrome P450 of the CYP51 family that catalyzes the first postcyclization step in ergosterol biosynthesis and is inhibited by triazole drugs. The structures reveal a well-ordered N-terminal amphipathic helix preceding a putative transmembrane helix that would constrain the catalytic domain orientation to lie partly in the lipid bilayer. The structures locate the substrate lanosterol, identify putative substrate and product channels, and reveal constrained interactions with triazole antifungal drugs that are important for drug design and understanding drug resistance.


Asunto(s)
Dominio Catalítico/genética , Sistema Enzimático del Citocromo P-450/química , Membrana Dobles de Lípidos/metabolismo , Modelos Moleculares , Conformación Proteica , Proteínas de Saccharomyces cerevisiae/química , Cromatografía de Afinidad , Cromatografía en Gel , Cristalización
2.
Proc Natl Acad Sci U S A ; 107(40): 17164-9, 2010 Oct 05.
Artículo en Inglés | MEDLINE | ID: mdl-20855585

RESUMEN

Aquaporins are transmembrane channels that facilitate the permeation of water and small, uncharged amphipathic molecules across cellular membranes. One distinct aquaporin subfamily contains pure water channels, whereas a second subfamily contains channels that conduct small alditols such as glycerol, in addition to water. Distinction between these substrates is central to aquaporin function, though the contributions of protein structural motifs required for selectivity are not yet fully characterized. To address this question, we sequentially engineered three signature amino acids of the glycerol-conducting subfamily into the Escherichia coli water channel aquaporin Z (AqpZ). Functional analysis of these mutant channels showed a decrease in water permeability but not the expected increase in glycerol conduction. Using X-ray crystallography, we determined the atomic resolution structures of the mutant channels. The structures revealed a channel surprisingly similar in size to the wild-type AqpZ pore. Comparison with measured rates of transport showed that, as the size of the selectivity filter region of the channel approaches that of water, channel hydrophilicity dominated water conduction energetics. In contrast, the major determinant of selectivity for larger amphipathic molecules such as glycerol was channel cross-section size. Finally, we find that, although the selectivity filter region is indeed central to substrate transport, other structural elements that do not directly interact with the substrates, such as the loop connecting helices M6 and M7, and the C loop between helices C4 and C5, play an essential role in facilitating selectivity.


Asunto(s)
Acuaporinas/química , Proteínas de Escherichia coli/química , Estructura Terciaria de Proteína , Secuencia de Aminoácidos , Acuaporinas/metabolismo , Permeabilidad de la Membrana Celular , Cristalografía por Rayos X , Escherichia coli/química , Escherichia coli/metabolismo , Proteínas de Escherichia coli/metabolismo , Glicerol/metabolismo , Modelos Moleculares , Datos de Secuencia Molecular , Proteolípidos/química , Proteolípidos/metabolismo , Agua/metabolismo
3.
Proc Natl Acad Sci U S A ; 107(24): 11038-43, 2010 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-20534468

RESUMEN

Resistance nodulation cell division (RND)-based efflux complexes mediate multidrug and heavy-metal resistance in many Gram-negative bacteria. Efflux of toxic compounds is driven by membrane proton/substrate antiporters (RND protein) in the plasma membrane, linked by a membrane fusion protein (MFP) to an outer-membrane protein. The three-component complex forms an efflux system that spans the entire cell envelope. The MFP is required for the assembly of this complex and is proposed to play an important active role in substrate efflux. To better understand the role of MFPs in RND-driven efflux systems, we chose ZneB, the MFP component of the ZneCAB heavy-metal efflux system from Cupriavidus metallidurans CH34. ZneB is shown to be highly specific for Zn(2+) alone. The crystal structure of ZneB to 2.8 A resolution defines the basis for metal ion binding in the coordination site at a flexible interface between the beta-barrel and membrane proximal domains. The conformational differences observed between the crystal structures of metal-bound and apo forms are monitored in solution by spectroscopy and chromatography. The structural rearrangements between the two states suggest an active role in substrate efflux through metal binding and release.


Asunto(s)
Proteínas Bacterianas/química , Proteínas Bacterianas/metabolismo , Proteínas Portadoras/química , Proteínas Portadoras/metabolismo , Proteínas de Transporte de Catión/química , Proteínas de Transporte de Catión/metabolismo , Proteínas de la Fusión de la Membrana/química , Proteínas de la Fusión de la Membrana/metabolismo , Zinc/metabolismo , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Proteínas Bacterianas/genética , Sitios de Unión , Proteínas Portadoras/genética , Proteínas de Transporte de Catión/genética , Cristalografía por Rayos X , Cupriavidus/efectos de los fármacos , Cupriavidus/genética , Cupriavidus/metabolismo , Farmacorresistencia Bacteriana , Proteínas de la Fusión de la Membrana/genética , Metales Pesados/toxicidad , Modelos Moleculares , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Proteínas Mutantes/química , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , Filogenia , Conformación Proteica , Estructura Terciaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homología de Secuencia de Aminoácido , Espectroscopía Infrarroja por Transformada de Fourier
4.
PLoS Biol ; 1(3): E72, 2003 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-14691544

RESUMEN

Aquaporins are a family of water and small molecule channels found in organisms ranging from bacteria to animals. One of these channels, the E. coli protein aquaporin Z (AqpZ), has been shown to selectively conduct only water at high rates. We have expressed, purified, crystallized, and solved the X-ray structure of AqpZ. The 2.5 A resolution structure of AqpZ suggests aquaporin selectivity results both from a steric mechanism due to pore size and from specific amino acid substitutions that regulate the preference for a hydrophobic or hydrophilic substrate. This structure provides direct evidence on the molecular mechanisms of specificity between water and glycerol in this family of channels from a single species. It is to our knowledge the first atomic resolution structure of a recombinant aquaporin and so provides a platform for combined genetic, mutational, functional, and structural determinations of the mechanisms of aquaporins and, more generally, the assembly of multimeric membrane proteins.


Asunto(s)
Acuaporinas/química , Proteínas de Escherichia coli/química , Proteínas de la Membrana/química , Secuencia de Aminoácidos , Acuaporinas/aislamiento & purificación , Carbono/química , Membrana Celular/metabolismo , Cristalografía por Rayos X , Detergentes/farmacología , Escherichia coli/metabolismo , Proteínas de Escherichia coli/aislamiento & purificación , Glicerol/química , Hidrógeno/química , Proteínas de la Membrana/aislamiento & purificación , Modelos Moleculares , Conformación Molecular , Datos de Secuencia Molecular , Oxígeno/química , Unión Proteica , Conformación Proteica , Estructura Secundaria de Proteína , Proteínas Recombinantes/química , Homología de Secuencia de Aminoácido , Agua/química
5.
Structure ; 11(2): 147-54, 2003 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-12575934

RESUMEN

Malonyl-CoA:ACP transacylase (MAT), the fabD gene product of Streptomyces coelicolor A3(2), participates in both fatty acid and polyketide synthesis pathways, transferring malonyl groups that are used as extender units in chain growth from malonyl-CoA to pathway-specific acyl carrier proteins (ACPs). Here, the 2.0 A structure reveals an invariant arginine bound to an acetate that mimics the malonyl carboxylate and helps define the extender unit binding site. Catalysis may only occur when the oxyanion hole is formed through substrate binding, preventing hydrolysis of the acyl-enzyme intermediate. Macromolecular docking simulations with actinorhodin ACP suggest that the majority of the ACP docking surface is formed by a helical flap. These results should help to engineer polyketide synthases (PKSs) that produce novel polyketides.


Asunto(s)
Ácidos Grasos/biosíntesis , Streptomyces/enzimología , Secuencia de Aminoácidos , Sitios de Unión , Datos de Secuencia Molecular , Alineación de Secuencia , Streptomyces/genética , Especificidad por Sustrato
6.
J Mol Biol ; 427(24): 3862-76, 2015 Dec 04.
Artículo en Inglés | MEDLINE | ID: mdl-26470919

RESUMEN

Human tRNA3(Lys) is the primer for reverse transcription of HIV; the 3' end is complementary to the primer-binding site on HIV RNA. The complementarity ends at the 18th base, A58, which in tRNA3(Lys) is modified to remove Watson-Crick pairing. Motivated to test the role of the modification in terminating the primer-binding sequence and thus limiting run-on transcription, we asked how the modification of RNA could be accomplished. tRNA m(1)A58 methyltransferase (m(1)A58 MTase) methylates N1 of A58, which is buried in the TΨC-loop of tRNA, from cofactor S-adenosyl-L-methionine. This conserved tRNA modification is essential for stability of initiator tRNA in Saccharomyces cerevisiae. Reported here, three structures of human tRNA m(1)A58 MTase in complex with human tRNA3(Lys) and the product S-adenosyl-L-homocysteine show a dimer of heterodimers in which each heterodimer comprises a catalytic chain, Trm61, and a homologous but noncatalytic chain, Trm6, repurposed as a tRNA-binding subunit that acts in trans; tRNAs bind across the dimer interface such that Trm6 from the opposing heterodimer brings A58 into the active site of Trm61. T-loop and D-loop are splayed apart showing how A58, normally buried in tRNA, becomes accessible for modification. This result has broad impact on our understanding of the mechanisms of modifying internal sites in folded tRNA. The structures serve as templates for design of inhibitors that could be used to test tRNA m(1)A58 MTase's impact on retroviral priming and transcription.


Asunto(s)
ARN de Transferencia/química , ARNt Metiltransferasas/química , Dominio Catalítico , Cristalografía por Rayos X , Humanos , Enlace de Hidrógeno , Metilación , Modelos Moleculares , Unión Proteica , Pliegue del ARN
7.
J Med Chem ; 56(13): 5446-55, 2013 Jul 11.
Artículo en Inglés | MEDLINE | ID: mdl-23710599

RESUMEN

N-[4-[2-Propyn-1-yl[(6S)-4,6,7,8-tetrahydro-2-(hydroxymethyl)-4-oxo-3H-cyclopenta[g]quinazolin-6-yl]amino]benzoyl]-l-γ-glutamyl-d-glutamic acid 1 (BGC 945, now known as ONX 0801), is a small molecule thymidylate synthase (TS) inhibitor discovered at the Institute of Cancer Research in London. It is licensed by Onyx Pharmaceuticals and is in phase 1 clinical studies. It is a novel antifolate drug resembling TS inhibitors plevitrexed and raltitrexed that combines enzymatic inhibition of thymidylate synthase with α-folate receptor-mediated targeting of tumor cells. Thus, it has potential for efficacy with lower toxicity due to selective intracellular accumulation through α-folate receptor (α-FR) transport. The α-FR, a cell-surface receptor glycoprotein, which is overexpressed mainly in ovarian and lung cancer tumors, has an affinity for 1 similar to that for its natural ligand, folic acid. This study describes a novel synthesis of 1, an X-ray crystal structure of its complex with Escherichia coli TS and 2'-deoxyuridine-5'-monophosphate, and a model for a similar complex with human TS.


Asunto(s)
Inhibidores Enzimáticos/farmacología , Antagonistas del Ácido Fólico/farmacología , Quinazolinas/farmacología , Timidilato Sintasa/antagonistas & inhibidores , Cristalografía por Rayos X , Diseño de Fármacos , Inhibidores Enzimáticos/síntesis química , Inhibidores Enzimáticos/metabolismo , Escherichia coli/enzimología , Proteínas de Escherichia coli/antagonistas & inhibidores , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Antagonistas del Ácido Fólico/síntesis química , Antagonistas del Ácido Fólico/metabolismo , Humanos , Modelos Químicos , Modelos Moleculares , Estructura Molecular , Neoplasias/enzimología , Neoplasias/patología , Unión Proteica , Estructura Terciaria de Proteína , Quinazolinas/síntesis química , Quinazolinas/metabolismo , Timidilato Sintasa/química , Timidilato Sintasa/metabolismo
8.
J Mol Biol ; 385(3): 820-30, 2009 Jan 23.
Artículo en Inglés | MEDLINE | ID: mdl-19061901

RESUMEN

A medium-throughput approach is used to rapidly identify membrane proteins from a eukaryotic organism that are most amenable to expression in amounts and quality adequate to support structure determination. The goal was to expand knowledge of new membrane protein structures based on proteome-wide coverage. In the first phase, membrane proteins from the budding yeast Saccharomyces cerevisiae were selected for homologous expression in S. cerevisiae, a system that can be adapted to expression of membrane proteins from other eukaryotes. We performed medium-scale expression and solubilization tests on 351 rationally selected membrane proteins from S. cerevisiae. These targets are inclusive of all annotated and unannotated membrane protein families within the organism's membrane proteome. Two hundred seventy-two targets were expressed, and of these, 234 solubilized in the detergent n-dodecyl-beta-D-maltopyranoside. Furthermore, we report the identity of a subset of targets that were purified to homogeneity to facilitate structure determinations. The extensibility of this approach is demonstrated with the expression of 10 human integral membrane proteins from the solute carrier superfamily. This discovery-oriented pipeline provides an efficient way to select proteins from particular membrane protein classes, families, or organisms that may be more suited to structure analysis than others.


Asunto(s)
Proteínas de la Membrana/química , Proteínas de Saccharomyces cerevisiae/química , Cromatografía de Afinidad , Cromatografía en Gel , Humanos , Proteínas de la Membrana/aislamiento & purificación , Plásmidos , Señales de Clasificación de Proteína , Proteínas de Saccharomyces cerevisiae/aislamiento & purificación , Solubilidad
9.
Nat Protoc ; 4(5): 619-37, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-19360018

RESUMEN

Protein crystallography is used to generate atomic resolution structures of protein molecules. These structures provide information about biological function, mechanism and interaction of a protein with substrates or effectors including DNA, RNA, cofactors or other small molecules, ions and other proteins. This technique can be applied to membrane proteins resident in the membranes of cells. To accomplish this, membrane proteins first need to be either heterologously expressed or purified from a native source. The protein has to be extracted from the lipid membrane with a mild detergent and purified to a stable, homogeneous population that may then be crystallized. Protein crystals are then used for X-ray diffraction to yield atomic resolution structures of the desired membrane protein target. Below, we present a general protocol for the growth of diffraction quality membrane protein crystals. The process of protein crystallization is highly variable, and obtaining diffraction quality crystals can require weeks to months or even years in some cases.


Asunto(s)
Cristalización/métodos , Cristalografía por Rayos X , Proteínas de la Membrana/química , Cromatografía de Afinidad , Cromatografía en Gel , Clonación Molecular , Detergentes , Escherichia coli/genética , Proteínas de la Membrana/genética , Proteínas de la Membrana/aislamiento & purificación , Estructura Terciaria de Proteína , Solubilidad
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