Early childhood language memory in the speech perception of international adoptees.

It is as yet unclear whether the benefits of early linguistic experiences can be maintained without at least some minimal continued exposure to the language. This study compared 12 adults adopted from Korea to the US as young children (all but one prior to age one year) to 13 participants who had no prior exposure to Korean to examine whether relearning can aid in accessing early childhood language memory. All 25 participants were recruited and tested during the second week of first-semester college Korean language classes. They completed a language background questionnaire and interview, a childhood slang task and a Korean phoneme identification task. Results revealed an advantage for adoptee participants in identifying some Korean phonemes, suggesting that some components of early childhood language memory can remain intact despite many years of disuse, and that relearning a language can help in accessing such a memory.

Conceptualization and measurement of birth family thoughts for adolescents and adults adopted transnationally.

For adopted individuals, understanding the role of birth family is an important part of developing a coherent life narrative. However, there is limited empirical research on this aspect of the adoption experience. We introduce a new construct, birth family thoughts, that captures a sense of curiosity about birth family, and describe the development of an accompanying brief self-report measure, the Birth Family Thoughts Scale (BFTS). Across 4 studies of transnationally adopted Korean American adolescents, emerging adults, and adults who were adopted before the age of 3 (ncombined = 546), we found strong support for a 1-factor structure using exploratory and confirmatory factor analyses, and good internal consistency and test-retest reliability. Convergent validity was generally supported. The BFTS was positively related to measures of adoption- and ethnicity-related constructs, although there were a few inconsistencies between studies and measures. Discriminant validity also was generally supported. We found no evidence for the BFTS being related to a poor adoptive family situation or an indication of psychopathology. We did find some evidence of the BFTS relating to internalizing and externalizing symptoms. Furthermore, while the BFTS was unrelated to travel to Korea, it was correlated with visiting an orphanage in Korea. It was also related to initiating a birth family search in Study 1, but not in Studies 2 or 3. We discuss the importance of considering birth family thoughts across the life span and with other populations, as well as the limitations of the current study including sampling issues inherent in working with hard-to-reach populations. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

Active immunoprophylaxis with a synthetic DNA-encoded monoclonal anti-respiratory syncytial virus scFv-Fc fusion protein confers protection against infection and durable activity.

Respiratory Syncytial virus (RSV) is a major threat to many vulnerable populations. There are currently no approved vaccines, and RSV remains a high unmet global medical need. Here we describe the employment of a novel synthetic DNA-encoded antibody technology platform to develop and deliver an engineered human DNA-encoded monoclonal antibody (dMAbTM) targeting the fusion protein (F) of RSV as a new approach to prevention or therapy of at risk populations. In in vivo models, a single administration of synthetic DNA-encoding the single-chain fragment variable-constant fragment (scFv-Fc) RSV-F dMAb resulted in robust and durable circulating levels of a functional antibody systemically and in mucosal tissue. In cotton rats, which are the gold-standard animals to model RSV infection, we observed sustained scFv-Fc RSV-F dMAb in the sera and lung-lavage samples, demonstrating the potential for both long-lasting immunity to RSV and effective biodistribution. The scFv-Fc RSV-F dMAb harbored in the sera exhibited RSV antigen-specific binding and potent viral neutralizing activity. Importantly, in vivo delivery of synthetic DNA-encoding, the scFv-Fc RSV-F dMAb protected animals against viral challenge. Our findings support the significance of dMAbs as a potential platform technology for durable protection against RSV disease.

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination.

The guinea pig has played a pivotal role as a relevant small animal model in the development of vaccines for infectious diseases such as tuberculosis, influenza, diphtheria, and viral hemorrhagic fevers. We have demonstrated that plasmid-DNA (pDNA) vaccine delivery into the skin elicits robust humoral responses in the guinea pig. However, the use of this animal to model immune responses was somewhat limited in the past due to the lack of available reagents and protocols to study T cell responses. T cells play a pivotal role in both immunoprophylactic and immunotherapeutic mechanisms. Understanding T cell responses is crucial for the development of infectious disease and oncology vaccines and accommodating delivery devices. Here we describe an interferon-gamma (IFN-γ) enzyme-linked immunospot (ELISpot) assay for guinea pig peripheral blood mononuclear cells (PBMCs). The assay enables researchers to characterize vaccine-specific T-cell responses in this important rodent model. The ability to assay cells isolated from the peripheral blood provides the opportunity to track immunogenicity in individual animals.

Delineating the Cellular Mechanisms Associated with Skin Electroporation.

The immune responses elicited following delivery of DNA vaccines to the skin has previously been shown to be significantly enhanced by the addition of electroporation (EP) to the treatment protocol. Principally, EP increases the transfection of plasmid DNA (pDNA) into the resident skin cells. In addition to increasing the levels of in vivo transfection, the physical insult induced by EP is associated with activation of innate pathways which are believed to mediate an adjuvant effect, further enhancing DNA vaccine responses. We investigated the possible mechanisms associated with this adjuvant effect, primarily focusing on the cell death pathways associated with the skin EP procedure independent of pDNA delivery. Using the minimally invasive CELLECTRA®-3P intradermal electroporation device that penetrates the epidermal and dermal layers of the skin, we have investigated apoptotic and necrotic cell death in relation to the vicinity of the electrode needles and electric field generated. Employing the well-established terminal deoxynucleotidyl transferase nick-end labeling assay, we detected apoptosis beginning as early as one hour after EP and peaking at the 4 h time point. The majority of the apoptotic events were detected in the epidermal region directly adjacent to the electrode needle. Using a novel propidium iodide in vivo necrotic cell death assay, we detected necrotic events concentrated in the epidermal region adjacent to the electrode. Furthermore, we detected upregulation of calreticulin expression on skin cells after EP, thus labeling these cells for uptake by dendritic cells and macrophages. These results allow us to delineate the cell death mechanisms occurring in the skin following intradermal EP independently of pDNA delivery. We believe these events contribute to the adjuvant effect observed following electroporation at the skin treatment site.

Characterization of guinea pig T cell responses elicited after EP-assisted delivery of DNA vaccines to the skin.

The skin is an ideal target tissue for vaccine delivery for a number of reasons. It is highly accessible, and most importantly, enriched in professional antigen presenting cells. Possessing strong similarities to human skin physiology and displaying a defined epidermis, the guinea pig is an appropriate model to study epidermal delivery of vaccine. However, whilst we have characterized the humoral responses in the guinea pig associated with skin vaccine protocols we have yet to investigate the T cell responses. In response to this inadequacy, we developed an IFN-γ ELISpot assay to characterize the cellular immune response in the peripheral blood of guinea pigs. Using a nucleoprotein (NP) influenza pDNA vaccination regimen, we characterized host T cell responses. After delivery of the DNA vaccine to the guinea pig epidermis we detected robust and rapid T cell responses. The levels of IFN-γ spot-forming units averaged approximately 5000 per million cells after two immunizations. These responses were broad in that multiple regions across the NP antigen elicited a T cell response. Interestingly, we identified a number of NP immunodominant T cell epitopes to be conserved across an outbred guinea pig population, a phenomenon which was also observed after immunization with a RSV DNA vaccine. We believe this data enhances our understanding of the cellular immune response elicited to a vaccine in guinea pigs, and globally, will advance the use of this model for vaccine development, especially those targeting skin as a delivery site.

Holding on to childhood language memory.

While early language experience seems crucial for mastering phonology, it remains unclear whether there are lasting benefits of speaking a language regularly during childhood if the quantity and quality of speaking drop dramatically after childhood. This study explored the accessibility of early childhood language memory. Specifically, it compared perception and production of Korean speech sounds by childhood speakers who had spoken Korean regularly for a few years during childhood to those of two other groups: (1) childhood hearers who had heard Korean regularly during childhood but had spoken Korean minimally, if at all; and (2) novice learners. All three groups were enrolled in first-year college Korean language classes. Childhood speakers were also compared to native speakers of Korean to see how native-like they were. The results revealed measurable long-term benefits of childhood speaking experience, underscoring the importance of early language experience, even if such experience diminishes dramatically beyond childhood.

Trends in publication of nursing informatics research.

We analyzed 741 journal articles on nursing informatics published in 7 biomedical/nursing informatics journals and 6 nursing journals from 2005 to 2013 to begin to understand publication trends in nursing informatics research and identify gaps. We assigned a research theme to each article using AMIA 2014 theme categories and normalized the citation counts using time from publication. Overall, nursing informatics research covered a broad spectrum of research topics in biomedical informatics and publication topics seem to be well aligned with the high priority research agenda identified by the nursing informatics community. The research themes with highest volume of publication were Clinical Workflow and Human Factors, Consumer Informatics and Personal Health Records, and Clinical Informatics, for which an increasing trend in publication was noted. Articles on Informatics Education and Workforce Development; Data Mining, NLP, Information Extraction; and Clinical Informatics showed steady and high volume of citations.

Salvaging a Childhood Language.

Childhood experience with a language seems to help adult learners speak it with a more native-like accent. Can analogous benefits be found beyond phonology? This study focused on adult learners of Spanish who had spoken Spanish as their native language before age 7 and only minimally, if at all, thereafter until they began to re-learn Spanish around age 14 years. They were compared with native speakers, childhood overhearers, and typical late-second-language (L2)-learners of Spanish. Both childhood speakers and overhearers spoke Spanish with a more native-like accent than typical late-L2-learners. On grammar measures, childhood speakers-although far from native-like-reliably outperformed childhood overhearers as well as typical late-L2-learners. These results suggest that while simply overhearing a language during childhood could help adult learners speak it with a more native-like phonology, speaking a language regularly during childhood could help re-learners use it with more native-like grammar as well as phonology.

Overhearing a language during childhood.

Despite its significance for understanding of language acquisition, the role of childhood language experience has been examined only in linguistic deprivation studies focusing on what cannot be learned readily beyond childhood. This study focused instead on long-term effects of what can be learned best during childhood. Our findings revealed that adults learning a language speak with a more native like accent if they overheard the language regularly during childhood than if they did not. These findings have important implications for understanding of language-learning mechanisms and heritage-language acquisition.

Production benefits of childhood overhearing.

The current study assessed whether overhearing Spanish during childhood helps later Spanish pronunciation in adulthood. Our preliminary report based on a subset of the data [Au et al., Psychol. Sci. 13, 238-243 (2002)] revealed that adults who overheard Spanish during childhood had better Spanish pronunciation, but not better morphosyntax, than adult learners of Spanish who had no childhood experience with Spanish. We now present data from the full sample with additional morphosyntax and pronunciation assessments, as well as measures to help rule out possible confounding prosodic factors such as speech rate, phrasing, and stress placement. Three groups of undergraduates were compared: 15 Spanish-English bilinguals (native Spanish speakers), 15 late learners of Spanish who overheard Spanish during childhood (childhood overhearers), 15 late learners of Spanish who had no regular experience with Spanish until middle or high school (typical late L2 learners). Results confirmed a pronunciation advantage for the childhood overhearers over the typical late L2 learners on all measures: phonetic analyses (VOT and degree of lenition), accent ratings (phoneme and story production), but no benefit in morphosyntax. Importantly, the pronunciation advantage did not seem attributable to prosodic factors. These findings illustrate the specificity of overhearers' advantage to phonological production.