RESUMEN
Mechanical properties of the extracellular matrix (ECM) have profound effects on cellular functions. Here, we applied novel photosensitive polydimethylsiloxane (photoPDMS) chemistry to create photosensitive, biocompatible photoPDMS as a rigidity-tunable material for study of mechanoresponsive cellular behaviors. By modulating the PDMS cross-linker to monomer ratio, UV light exposure time, and postexposure baking time, we achieved a broad range of bulk Young's modulus for photoPDMS from 0.027 to 2.48 MPa. Biocompatibility of photoPDMS was assayed, and no significant cytotoxic effect was detected as compared to conventional PDMS. We demonstrated that the bulk Young's modulus of photoPDMS could impact cell morphology, adhesion formation, cytoskeletal structure, and cell proliferation. We further fabricated photoPDMS micropost arrays for multiscale study of mechanoresponsive cellular behaviors. Our results suggested that adherent cells could sense and respond to changes of substrate rigidity at a subfocal adhesion resolution. Together, we demonstrated the potential of photoPDMS as a photosensitive and rigidity-tunable material for mechanobiology studies.
Asunto(s)
Materiales Biocompatibles/química , Dimetilpolisiloxanos/química , Fibroblastos/citología , Fármacos Fotosensibilizantes/química , Rayos Ultravioleta , Animales , Adhesión Celular , Proliferación Celular , Células Cultivadas , Citoesqueleto/química , Ratones , Estructura Molecular , Células 3T3 NIHRESUMEN
AIM: To demonstrate an orthodontic approach for a patient with Treacher-Collins syndrome. METHODS: After growth observation and eruption of permanent teeth, a multibracket fixed appliance was applied for alignment of the teeth and bite closing. After 4 years 6 months of active treatment, Hawley retainers were used for stabilization. CONCLUSION: An orthodontic treatment in a patient with Treacher-Collins syndrome was completed with a fairly satisfactory result.