Comparison of surface roughness of additively manufactured implant-supported interim crowns fabricated with different print orientations.

PURPOSE: To assess the influence of print orientation on the surface roughness of implant-supported interim crowns manufactured by using digital light processing (DLP) 3D printing procedures. MATERIALS AND METHODS: An implant-supported maxillary right premolar full-contour crown was obtained. The interim restoration design was used to fabricate 30 specimens with 3 print orientations (0, 45, and 90 degrees) using an interim resin material (GC Temp PRINT) and a DLP printer (Asiga MAX UV) (n = 10). The specimens were manufactured, and each was cemented to an implant abutment with autopolymerizing composite resin cement (Multilink Hybrid Abutment). Surface roughness was assessed on the buccal surface of the premolar specimen by using an optical measurement system (InfiniteFocusG5 plus). The data were analyzed with a Shapiro-Wilk test, resulting in a normal distribution. One-way ANOVA and the Tukey HSD tests were selected (α = 0.05). RESULTS: Statistically significant discrepancies were found in the surface roughness mean values among the groups tested (p < 0.001). The lowest mean ± standard deviation surface roughness was found with the 90-degree group (1.2 ± 0.36 µm), followed by the 0-degree orientation (2.23 ± 0.18 µm) and the 45-degree group (3.18 ± 0.31 µm). CONCLUSIONS: Print orientation parameter significantly impacted the surface roughness of the implant-supported interim crowns manufactured by using the additive procedures tested.

Non-chlorophyllous and crypto-chlorophyllous fern spores differ in their mobilisation of fatty acids during priming.

During fern spore germination, lipid hydrolysis primarily provides the energy to activate their metabolism. In this research, fatty acids (linoleic, oleic, palmitic and stearic) were quantified in the spores exposed or not to priming (hydration-dehydration treatments). Five fern species were investigated, two from xerophilous shrubland and three from a cloud forest. We hypothesised that during the priming hydration phase, the fatty acids profile would change in concentration, depending on the spore type (non-chlorophyllous and crypto-chlorophyllous). The fatty acid concentration was determined by gas chromatograph-mass spectrometer. Chlorophyll in spores was vizualised by epifluorescence microscopy and quantified by high-resolution liquid chromatography with a DAD-UV/Vis detector. Considering all five species and all the treatments, the oleic acid was the most catabolised. After priming, we identified two patterns in the fatty acid metabolism: (1) in non-chlorophyllous species, oleic, palmitic, and linoleic acids were catabolised during imbibition and (2) in crypto-chlorophyllous species, these fatty acids increased in concentration. These patterns suggest that crypto-chlorophyllous spores with homoiochlorophylly (chlorophyll retained after drying) might not require the assembly of new photosynthetic apparatus during dark imbibition. Thus, these spores might require less energy from pre-existing lipids and less fatty acids as 'building blocks' for cell membranes than non-chlorophyllous spores, which require de novo synthesis and structuring of the photosynthetic apparatus.

Influence of arch location and scanning pattern on the scanning accuracy, scanning time, and number of photograms of complete-arch intraoral digital implant scans.

OBJECTIVES: To measure the influence of arch location and scanning pattern on the accuracy, scanning time, and number of photograms of complete-arch implant scans acquired using an intraoral scanner (IOS). MATERIALS AND METHODS: A maxillary (maxillary group) and mandibular (mandibular group) model with 6 implant abutments on each cast was digitized using a desktop scanner (control scans). Six subgroups were created based on the scanning pattern used to acquire the scans using an IOS (Trios 4): occluso-buccal-lingual (OBL subgroup), occluso-linguo-buccal (OLB subgroup), bucco-linguo-occlusal (BLO subgroup), linguo-buccal-occlusal (LBO subgroup), zigzag (ZZ subgroup), and circumferential (C subgroup). The control scans were used as a reference to measure the discrepancy with the experimental scans calculating the root mean square error. Two-way ANOVA and the pairwise comparison Tukey tests were used to analyze the data (α = .05). RESULTS: Significant discrepancies in trueness (p < .001), precision (p < .001), scanning time (p < .001), and number of photograms (p < .001) were found. The maxillary group obtained poorer trueness and precision values, higher scanning times, and a larger number of photograms than the mandibular group. The C subgroup obtained the best trueness and precision values, but was not significantly different from the OLB, BLO, and LBO subgroups. The ZZ subgroup obtained the worst trueness and precision values (p < .05). The C subgroup obtained the lowest scanning time and number of photograms (p < .05). CONCLUSIONS: Arch location and scanning pattern influenced scanning accuracy, scanning time, and number of photograms of complete-arch implant scans.

Techniques to improve the accuracy of complete arch implant intraoral digital scans: A systematic review.

STATEMENT OF PROBLEM: The best method of optimizing the accuracy of complete arch intraoral digital scans is still unclear. For instance, the location of the scan bodies can be significantly distorted with respect to their actual positions, which would lead to a nonpassive fit of the definitive prosthesis. PURPOSE: The purpose of this systematic review was to analyze available techniques for improving the accuracy of digital scans in implant-supported complete arch fixed prostheses. MATERIAL AND METHODS: Three databases (Medline, Embase, and Google Scholar) were searched, and the results obtained were supplemented by a hand search. Specific descriptors identified techniques whose objective were to increase the accuracy of digital scans in implant-supported complete arch fixed prostheses. Titles and abstracts were screened by 2 independent reviewers, and unclear results were discussed with a third independent reviewer. A qualitative analysis based on procedural parameters was used. The interexaminer agreements of both were assessed by the Cohen kappa statistic, and the Risk of Bias Tool was used to assess the risk of bias across the studies. RESULTS: A total of 17 techniques matching the inclusion criteria were evaluated. Higher accuracy but also differences regarding the need for supplementary devices, number of intraoral scans, and time consumption of clinical and software program steps were observed compared with the conventional digital scanning protocol. The use of a splinting device was common to most of the studies. The outcome variables for the evaluation of the effectiveness of these protocols were heterogeneous. CONCLUSIONS: The use of additional techniques during intraoral scanning can improve accuracy in implant-supported complete arch fixed prostheses. However, higher complexity for those procedures should be expected.

Accuracy, scanning time, and patient satisfaction of stereophotogrammetry systems for acquiring 3D dental implant positions: A systematic review.

PURPOSE: To evaluate accuracy, scanning time, and patient satisfaction of photogrammetry (PG) systems for recording the 3D position of dental implants. MATERIAL AND METHODS: A literature search was completed in five databases: PubMed/Medline, Scopus, Embase, World of Science, and Cochrane. A manual search was also conducted. Studies reporting the use of commercially available PG systems were included. Two investigators evaluated the studies independently by applying the Joanna Briggs Institute critical appraisal. A third examiner was consulted to resolve any lack of consensus. RESULTS: A total of 14 articles were included: 3 in vivo, 6 in vitro, and 6 case report manuscripts. One clinical study evaluated trueness, another one tested precision, and the third one assessed impression time and patient and operator satisfaction. All the in vitro studies evaluated the trueness and precision of a PG system. Additionally, all the reviewed studies investigated completely edentulous conditions with multiple implants. The number of placed implants per arch among the reviewed clinical studies varied from 4 to 8 implants, while the number of implants placed on the reference casts included 4, 5, 6, or 8 implants. Not all the studies compared the accuracy of PG systems with conventional impression methods, using intraoral scanners as additional experimental groups. For the PIC system, trueness ranged from 10 to 49 µm and precision ranged from 5 to 65 µm. For the iCam4D system, trueness ranged from 24 to 77 µm and the precision value ranged from 2 to 203 µm. CONCLUSIONS: PG systems may provide a reliable alternative for acquiring the 3D position of dental implants. However, this conclusion should be interpreted carefully, as one study reported a mean precision value of one PG system higher than the clinically acceptable discrepancy. Lower scanning time and higher patient and operator satisfaction have been reported when compared with conventional techniques. Further studies are needed to increase the evidence regarding the accuracy, scanning time, and patient and operator satisfaction of the commercially available PG systems.

Accuracy, scanning time, and number of photograms of various scanning patterns for the extraoral digitalization of complete dentures by using an intraoral scanner.

STATEMENT OF PROBLEM: The use of intraoral scanners (IOSs) has been described as a method of digitizing complete dentures (CDs) extraorally; however, accuracy, scanning time, and number of photograms remain unclear. PURPOSE: The purpose of this in vitro study was to assess the accuracy, scanning time, and number of photograms of different scanning patterns for digitizing CDs extraorally with an IOS. MATERIAL AND METHODS: The virtual design of a maxillary and mandibular CD was used to manufacture milled CDs. The maxillary (Mx group) and mandibular (Mb group) CDs were digitized extraorally by using an IOS (Medit i500). Three subgroups were created depending on the scanning pattern: using the protocol of the IOS selected (Medit Link) (ML subgroup), using the technique recommended by the implant manufacturer (DIOnavi) (DIO subgroup), and using a custom method (C subgroup). The design of the CDs (reference files) was used to compare the discrepancy between each virtual design and the corresponding scans by using the root mean square (RMS) error. The scanning time and number of photograms were recorded. Two-way ANOVA and Tukey tests were used to analyze the trueness data. The Bartlett test was used to analyze the precision values (α=.05). RESULTS: The group (P<.001) was a significant predictor of trueness. The Mx group (RMS mean of 0.452 mm) obtained higher trueness than the Mb group (RMS mean of 0.536 mm). The Mx-C and Mb-DIO subgroups obtained the highest trueness, and the Mx-ML and Mb-ML subgroups showed the lowest trueness. Significant differences were found in precision among groups (P<.01) and across subgroups (P<.01). The Mb group obtained the highest precision mean (0.586 mm) and was significantly different from the Mx group that obtained the lowest precision mean (0.611 mm). The C subgroup obtained the highest precision mean (0.339 mm) and was significantly different from the DIO subgroup that obtained the lowest precision mean (0.425 mm). The group (P<.01) and scanning pattern (P<.01) were significant predictors of the scanning time and number of photograms obtained. The Mb group showed lower scanning time and number of photograms than the Mx group. CONCLUSIONS: The scanning patterns tested significantly influenced the trueness and precision values, scanning time, and number of photograms of the extraoral digitalization of maxillary and mandibular CDs by using the IOS tested.

Influence of implant angulation and clinical implant scan body height on the accuracy of complete arch intraoral digital scans.

STATEMENT OF PROBLEM: The accuracy of digital implant scans can be affected by the implant angulation, implant depth, or interimplant distance. However, studies analyzing intraoral scanning accuracy with different implant angulations and different scan body heights are scarce. PURPOSE: The purpose of this in vitro study was to determine the influence of the implant angulation and clinical implant scan body height on the accuracy of complete arch scans. MATERIAL AND METHODS: Two definitive implant casts with 6 implant analogs (Zimmer Biomet) were obtained: 1 cast had all the implant analogs parallel (GP group), and 1 cast had the implant analogs with divergence of up to 30 degrees (GD group). A coordinate measurement machine (Global Evo 09.15.08) was used to measure the positions of the implant analogs. Each group was divided into 3 subgroups depending on the clinical implant scan body height: 10, 6, and 3 mm. An implant scan body (Elos Accurate Scan Body Brånemark system) was positioned on each implant analog. A total of 10 scans of each subgroup were recorded by using an intraoral scanner (TRIOS 3). Each STL file obtained was imported into a reverse engineering software program (Geomagic), and linear and angular Euclidean measurements were obtained. The Euclidean calculations between the implant analog positions of the definitive implant casts were used as a reference to calculate the discrepancies among the corresponding subgroups. The Kolmogorov-Smirnov test revealed that the lineal measurements were not normally distributed, so the Kruskal-Wallis and pairwise comparison Dunn tests were used (α=.05). The Kolmogorov-Smirnov test revealed that the angular measurements were normally distributed. Therefore, the 2-way ANOVA and pairwise comparison Tukey tests were used (α=.05). RESULTS: The Kruskal-Wallis test revealed significant differences in the linear Euclidean medians between the GP and GD groups with different clinical implant scan body heights (H(5)=23.18, P<.001). Significant differences in the linear Euclidean medians were computed between the GP-6 and GD-10 subgroups (P=.009), GD-3 and GD-6 subgroups (P=.029), and GD-3 and GD-10 subgroups (P=.001). Two-way ANOVA revealed that the implant angulation (F(1, 3.3437)=28.93, P<.001) and clinical implant scan body height (F(2, 0.4358)=3.77, P=.029) were significant predictors of discrepancies in the angular measurement. CONCLUSIONS: Implant angulation and clinical scan body height influenced scanning accuracy. The lowest clinical implant scan body height tested had the lowest accuracy in both parallel and angulated implants, but statistically significant differences were found only in the angulated group.

Intraoral Digital Scans for Fabricating Tooth-Supported Prostheses Using a Custom Intraoral Scan Body.

This article describes a technique to assist with intraoral digital scans for fabricating tooth-supported prostheses by using a custom intraoral scan body when the extension of the scan or the clinical characteristics might compromise the reliability of the intraoral digital scan. A preliminary intraoral scan of the tooth preparations is used to design a custom intraoral scan body which is manufactured using polymethylmethacrylate and a 5-axis milling machine. A low-viscosity polyvinyl siloxane impression of the tooth preparations is obtained using the custom intraoral scan body. Subsequently, the custom intraoral scan body is digitized using an intraoral scanner. A design software program is used to align the digitized custom intraoral scan body with the preliminary intraoral scan to obtain the definitive virtual cast. This technique aims to reduce manual conventional laboratory procedures such as pouring dental impression or die trimming which might minimize inaccuracies on the virtual definitive cast.

Lifestyle Modification Improves Insulin Resistance and Carotid Intima-Media Thickness in a Metabolically Healthy Obese Prepubescent Population.

OBJECTIVES: The aim of this study was to analyze effects of a 12-month lifestyle modification that involved a Mediterranean diet (MedDiet) and physical activity (PA) program in a population of metabolically healthy obese children (MHOCh). METHODS: We included a population of MHOCh with ≤1 of the following criteria: waist circumference and blood pressure ≥90 percentile, triglycerides >150 mg/dL, high-density lipoprotein-cholesterol (HDL-c) <40 mg/dL, or impaired fasting glucose. After 12 months of intensive lifestyle modification, anthropometric measurements, glycemic and lipid profiles, adherence to the MedDiet, energy intake, PA, body composition, and carotid intima-media thickness (cIMT) were analyzed. RESULTS: One hundred thirty-one MHOCh (70 boys and 61 girls; P = 0.65, age: 7.9 ±â€Š1.3 years, body mass index [BMI]: 24.7 ±â€Š3.5 kg/m2) were included. After 12 months of intervention, a significant decrease in standard deviation (SD) units of body weight (-0.5 ±â€Š0.1; P < 0.001) and BMI (-0.5 ±â€Š0.1; P < 0.001) were observed in the total population. A significant improvement in adherence to the MedDiet (+2 points) and a significant reduction in protein, fatty acids, total fat, and cholesterol intake in the entire population were observed. All participants did more moderate-vigorous PA, which led to a significant increase in lean and total mass and decrease in total fat. Significant improvements in the glycemic profile (insulin levels [-6.6 µIU/mL, P < 0.001] and HOMA index [-1.2, P < 0.001]) were observed. Participants with pathological cIMT values reduced this cardiovascular predictor to normal values. CONCLUSIONS: A 12-month lifestyle modification intervention involving weight loss with MedDiet and PA in MHOCh yielded improvements in MedDiet adherence, lipid intake, moderate-vigorous PA, body composition, insulin resistance, and cIMT.

Merging intraoral scans and CBCT: a novel technique for improving the accuracy of 3D digital models for implant-supported complete-arch fixed dental prostheses.

AIM: A technique for merging digital intraoral and CBCT scans for implant-supported complete-arch fixed dental prostheses (FDPs) is described. The aim is to improve the dimensional accuracy of intraoral scans in edentulous arches. MATERIALS AND METHOD: Two files are recorded: an intraoral scan and a CBCT scan, both obtained with scan bodies connected to the implants in the same position. The intraoral scan is then divided into several fragments and realigned, taking as reference the position of the implants recorded in the CBCT file. RESULTS: An improved intraoral digital model with corrected implant positions appropriate for complete-arch implant FDPs is generated. CONCLUSION: The methodology proposed can minimize possible intraoral scanning error and deliver more reliable digital impressions for implant-supported complete-arch FDPs.

Polymorphisms PSCA rs2294008, IL-4 rs2243250 and MUC1 rs4072037 are associated with gastric cancer in a high risk population.

Genetic variants are considered risk factors for gastric cancer. To date, 61 polymorphisms have been identified as associated with this disease. The aim of the present study was to analyze the association of some of those polymorphisms with GC in Chile. We performed a case-control study including 310 gastric cancer cases and 311 controls to assess the association of 36 single-nucleotide polymorphisms genotyped by Global Screening Array (GSA). Three polymorphisms was significantly associated: PSCA rs2294008 (allele model, OR = 1.49, 95%CI 1.17-1.88, P = 1.08 × 10-3), IL-4 rs2243250 (allele model, OR = 1.28, 95%CI 1.01-1.62, P = 0.04), and MUC1 rs4072037 (allele model, OR = 0.78, 95%CI 0.61-0.99, P = 0.04).PSCA rs2294008, IL-4 rs2243250 and MUC1 rs4072037 are associated with gastric cancer in Chile. It suggests that those polymorphisms could be used as biomarkers to assess the genetic risk for this cancer outside of the previously studied populations, not only for East Asians and Caucasians populations.

Guided implant scanning: A procedure for improving the accuracy of implant-supported complete-arch fixed dental prostheses.

This article describes a technique to improve the quality of digital intraoral scans for complete-arch prostheses by correcting the cumulative deviations that may arise with conventional digital methods. The approach involves an additional scan in which a reference-marked rigid splint of known dimensions is used to reduce the likelihood of such deviations. The scanned files are sectioned and best fit aligned to generate a more reliable definitive cast and consequently a better-fitting prosthesis.

Ontogenic synchronization of Bephratelloides cubensis, Annona macroprophyllata seeds and acetogenins from Annonaceae.

The seeds of Annona macroprophyllata Donn. Sm. contain idioblasts with toxic acetogenins, including laherradurin and rolliniastatin-2, in relatively high proportions. Both metabolites are cataloged as potent insecticides for several species, even so, the wasp Bephratelloides cubensis Ashmead fulfills almost its entire life cycle inside the seeds of this and other annonaceous species, to such a degree, that they constitute a strong selection pressure. In order to document the chemical relationship between the two species, it is reported for the first time in this paper the presence of idioblasts and acetogenins during the ontogenic development of the seeds of A. macroprophyllata, and contrasted with the development of B. cubensis. The results indicate that idioblasts with laherradurin and rolliniastatin-2 acetogenins are formed in the middle stages of the endospermic development, also that both acetogenins are biosynthesized simultaneously, and that their proportion is dependent on the degree of development. The acetogenins are present in high amounts that suppose a sufficient toxic barrier and, in this case, laherradurin is the most abundant (> 1000 µg g dry weight-1). The wasp B. cubensis only emerges from the seeds to copulate and returns for oviposition; its larval phase coincides with the appearance of acetogenins, so it feeds on the acetogenic endosperm. The absence of acetogenins in the tissues and excreta of the insect supposes a metabolization of the molecules, which would explain the tolerance to its toxicity.

Increased maize growth and P uptake promoted by arbuscular mycorrhizal fungi coincide with higher foliar herbivory and larval biomass of the Fall Armyworm Spodoptera frugiperda.

Most plant species naturally associate with arbuscular mycorrhizal fungi (AMF), which are known to promote crop nutrition and health in agroecosystems. However, information on how mycorrhizal associations affect plant biotic interactions that occur aboveground with foliar herbivores is limited and needs to be further addressed for the development of pest management strategies. With the objective to examine the influence of maize mycorrhizas on foliar herbivory caused by larvae of Spodoptera frugiperda, a serious pest in maize agroecosystems, we performed a fully factorial greenhouse pot experiment with three factors: Maize genotype (Puma and Milpal H318), AMF (with and without AMF, and without AMF with mineral P) and Insect herbivory (with and without S. frugiperda). Main results showed that inoculation with AMF improved plant growth and foliar P concentration, which coincided with increased foliar damage from herbivory and higher biomass of S. frugiperda larvae. A significant positive correlation between shoot P concentration and larval biomass was also observed. Finally, foliar herbivory by S. frugiperda slightly increased and decreased AMF root colonization in Puma and H318, respectively. In conclusion, our results show that maize plant benefits from AMF in terms of promotion of growth and nutrition, and may also increase the damage caused from insects by improving the food quality of maize leaves for larval growth, which seems to be linked to increased P uptake by the maize mycorrhizal association.

[Responses of the common bean (Phaseolus vulgaris L.) and Rhizobium tropici CIAT899 symbiosystem to induced allelopathy by Ipomoea purpurea L. Roth]. / Respuesta del simbiosistema frijol (Phaseolus vulgaris L.) y Rhizobium tropici CIAT899 ante el efecto alelopático de Ipomoea purpurea L. Roth.

Allelopathy is a phenomenon that involves the production of secondary metabolites that influence the growth of plants and microorganisms; however, this alellopathic effect has been scarcely studied on the rhizobia-legume symbiosis. The aims of this research were 1) to assess the allelopathic potential of aqueous extracts of Ipomoea purpurea L. Roth on seed germination and root length of common bean seedlings (Phaseolus vulgaris L.), 2) to determine its effects on the in vitro growth of Rhizobium tropici CIAT899, and 3) to evaluate the allelopathic potential of I. purpurea on the growth, nodulation and physiology of common bean plants inoculated with R. tropici. After 48h, 15% of the aqueous root extract of I. purpurea stimulated seed germination, whereas 4% of the aqueous shoot extracts stimulated such germination. Both the root or shoot extracts stimulated seed germination and e root length. In vitro growth of R. tropici was inhibited as a result of the application of both aqueous extracts. The presence of I. purpurea negatively affected both the growth and physiological responses of common bean plants, and this effect was attenuated after the inoculation of R. tropici; nevertheless, this allelopathic plant affected root nodulation. Our results suggest that the symbiosis of rhizobia and roots of common bean plants is an important element for attenuating the negative effects caused by the allelopathic plant.

Stereophotogrammetric impression making for polyoxymethylene, milled immediate partial fixed dental prostheses.

Immediate post-extraction and same-day placement of interim prostheses have increased patient acceptance of implant-supported prostheses. However, for immediate prostheses supported by multiple implants, meeting passive fit and esthetic standards is often challenging. In this clinical report, implant photogrammetry was combined with conventionally obtained digitized casts to prepare an interim, milled prosthesis from a polyoxymethylene (POM) disk, using computer-aided design and computer-aided manufacturing techniques. The following 2 conclusions were drawn. First, stereophotogrammetric scanning appears to be a reliable method for making impressions of immediate, implant-supported partial prostheses, and second, POM is suitable for preparing immediate interim screw-retained prosthetic implants.

Interim restoration using dynamic abutments to re-treat a single-implant crown with a labial angulation: A clinical report.

The cause of some peri-implant problems may be primarily attributable to the design of the prosthesis. A screw-retained interim implant may be advisable for reversibility and to avoid peri-implant cement, although screw retention may be difficult for maxillary anterior implants with a labial angulation. In the treatment described, a interim screw-retained crown was attached to a dynamic abutment with a lingual screw access hole to obtain a correctly fitting restoration.

Role of Nerve Growth Factor (NGF) and miRNAs in Epithelial Ovarian Cancer.

Ovarian cancer is the eighth most common cancer in women worldwide, and epithelial ovarian cancer (EOC) represents 90% of cases. Nerve growth factor (NGF) and its high affinity receptor tyrosine kinase A receptor (TRKA) have been associated with the development of several types of cancer, including EOC; both NGF and TRKA levels are elevated in this pathology. EOC presents high angiogenesis and several molecules have been reported to induce this process. NGF increases angiogenesis through its TRKA receptor on endothelial cells, and by indirectly inducing vascular endothelial growth factor expression. Other molecules controlled by NGF include ciclooxigenase-2, disintegrin and metalloproteinase domain-containing protein 17 (ADAM17) and calreticulin (CRT), proteins involved in crucial processes needed for EOC progression. These molecules could be modified through microRNA regulation, which could be regulated by NGF. MicroRNAs are the widest family of non-coding RNAs; they bind to 3'-UTR of mRNAs to inhibit their translation, to deadenilate or to degraded them. In EOC, a deregulation in microRNA expression has been described, including alterations of miR-200 family, cluster-17-92, and miR-23b, among others. Since the NGF-microRNA relationship in pathologies has not been studied, this review proposes that some microRNAs could be associated with NGF/TRKA activation, modifying protein levels needed for EOC progression.

Does Length, Diameter, or Bone Quality Affect Primary and Secondary Stability in Self-Tapping Dental Implants?

PURPOSE: Implant stability is a clinically valuable measurement of the strength of implant anchorage in the bone during placement and in the post-osseointegration period. This study aimed to determine 1) the effect of implant diameter and length and bone quality on measurements of primary and secondary stability (insertion torque [IT] and implant stability quotient [ISQ]), 2) the correlation between IT and primary and secondary ISQ, and 3) differences in ISQ in the post-osseointegration period (secondary stability) compared with immediate post-placement (primary) stability. PATIENTS AND METHODS: In this longitudinal clinical study, titanium self-tapping implants were inserted in edentulous patients. The implants were grouped according to 3 independent variables: length (10 and 11.5 mm), diameter (3.75 and 4.25 mm), and bone quality (Lekholm and Zarb classification) to analyze primary and secondary implant stability (outcome variables). Statistical analyses were performed using the Student t test for paired data, 1-way analysis of variance, and the Tukey procedure for multiple pairwise comparisons. RESULTS: Data were collected on 88 self-tapping implants inserted in 63 partially edentulous patients. IT and implant stability were affected by diameter (3.75-mm implants, 26.5-N/cm IT and 74.0 ISQ; 4.25-mm implants, 33.8-N/cm IT and 77.0 ISQ) and bone type (type 1 + 2, 34.86-N/cm IT and 77.4 ISQ; type 3, 27.09-N/cm IT and 75.6 ISQ; type 4, 20.63-N/cm IT and 70.5 ISQ; P < .01 for all comparisons). Secondary ISQ was affected by diameter only (77.41 for 3.75- vs 75.51 for 4.25-mm implants). IT correlated with primary ISQ (R = 0.56; P < .01), although no clear correlation with secondary stability was found. CONCLUSIONS: IT and primary ISQ in self-tapping implants differed in patients with different bone quality and implant diameter but did not differ between the 2 implant lengths compared in this study. Secondary stability was not substantially affected by any of these factors. Although IT was closely related to primary ISQ, it was unrelated to secondary ISQ. Very high primary ISQ values tended to decrease, whereas intermediate and low values tended to increase, in the transition to secondary stability.

Influence of the Use of Transepithelial Abutments vs. Titanium Base Abutments on Microgap Formation at the Dental Implant-Abutment Interface: An In Vitro Study.

This in vitro study aimed to assess the presence of microgaps at the implant-abutment interface in monolithic zirconia partial implant-supported fixed prostheses on transepithelial abutments versus Ti-base abutments. METHODS: Sixty conical connection dental implants were divided into two groups (n = 30). The control group consisted of three-unit bridge monolithic zirconia connected to two implants by a transepithelial abutment. The test group consisted of monolithic zirconia three-unit restoration connected to two implants directly by a titanium base (Ti-base) abutment. The sample was subjected to thermocycling (10,000 cycles at 5 °C to 55 °C, dwelling time 50 s) and chewing simulation (300,000 cycles, under 200 N at frequencies of 2 Hz, at a 30° angle). The microgap was evaluated at six points (mesiobuccal, buccal, distobuccal, mesiolingual, lingual, and distolingual) of each implant-abutment interface by using a scanning electron microscope (SEM). The data were analyzed using the Mann-Whitney U tests (p > 0.05). RESULTS: The SEM analysis showed a smaller microgap at the implant-abutment interface in the control group (0.270 µm) than in the test group (3.902 µm). Statistically significant differences were observed between both groups (p < 0.05). CONCLUSIONS: The use or not of transepithelial abutments affects the microgap size. The transepithelial abutments group presented lower microgap values at the interface with the implant than the Ti-base group in monolithic zirconia partial implant-supported fixed prostheses. However, both groups had microgap values within the clinically acceptable range.