Delivery devices for exposure of biological cells to nanosecond pulsed electric fields.

In this paper, delivery devices for nanosecond pulsed electric field exposure of biological samples in direct contact with electrodes or isolated are presented and characterized. They are based on a modified electroporation cuvette and two transverse electromagnetic cells (TEM cells). The devices were used to apply pulses with high intensity (4.5 kV) and short durations (3 and 13 ns). The delivery devices were electromagnetically characterized in the frequency and time domains. Field intensities of around 5, 0.5, and 12 MV m-1 were obtained by numerical simulations of the biological sample positioned in the three delivery devices. Two delivery systems had a homogenous electric field spatial distribution, and one was adapted to permit a highly localized exposure in the vicinity of a needle. Experimental biological investigations were carried out at different field intensities for five cancer cell lines. The results using flow cytometry showed that cells kept polarized mitochondrial membrane but lost plasma membrane integrity following a dose-response trend after exposure to different electric field intensities. Certain cell types (U87, MCF7) showed higher sensitivities to nsPEFs than other lines tested.

High-resolution measurements of the electric field at the streamer arrival to the cathode: a unification of the streamer-initiated gas-breakdown mechanism.

A time-correlated single-photon counting technique was used to verify the formation of a cathode-directed streamer inside the narrow cathode region following the interpulse phase of regular negative corona Trichel pulses in ambient air. A purely experimental approach was used to determine the spatiotemporal development of the electric field during the Trichel pulse rise with an extremely high resolution of 10 µm and tens of picoseconds. The results confirm the positive-streamer mechanism for Trichel pulse formation and provide supportive evidence for the hypothesis that the formation of a primary cathode-directed streamer occurs always in any streamer-initiated breakdown and prebreakdown phenomena associated with cathode spot formation.