RESUMEN
The efficiency of benfluron (B-F) was measured by determining the inhibition of cell proliferation in HeLa and V/79 cells. B-F induced an acute cytotoxic reaction in dependency on the concentration applied. A biphasic unbalanced growth was an integral part of this reaction. In connection with the decrease in cell proliferation which followed B-F treatment at micromolar concentrations, the protein:cell volume ratios increased while at nanomolar concentrations decreased. These variations must be taken into account when expressing and interpreting the results of cell metabolism. The glucose and amino acid metabolism of treated V/79 cells differed markedly when expressed as a function of cell number or as function of protein content per cell.
Asunto(s)
Antineoplásicos/farmacología , Supervivencia Celular/efectos de los fármacos , Fluorenos/farmacología , Animales , División Celular/efectos de los fármacos , Línea Celular , Células HeLa/citología , Células HeLa/efectos de los fármacos , Humanos , CinéticaRESUMEN
The relationship between proliferation and metabolism of four leukemia cell lines (BALL-1, JOK-1, Jurkat, and MOLT-4) in batch culture was studied. The maximum cell density (1.5-2.3 x 10(6) cells/ml) without change of medium was observed on days 6-8 of cultivation. At the same time, the original concentration of glucose in the medium (10 mmol/l) fell to 3.5-4 mmol/l. While BALL-1 and MOLT-4 cells, on day 4 of cultivation, converted 82% of glucose into lactate, on day 7 this value was 50%, or there was no lactate production (MOLT-4), respectively. On the other hand, the values of the coefficient of glycolysis showed that Jurkat and JOK cells converted also other compounds into lactate. Part of the utilized glutamine was employed by all four cell lines: 1. as a precursor of glutamic acid, and 2. as a source of energy. BALL-1 and JOK-1 cells converted part of arginine into ornithine. At the time when the proliferation of the cells ceased, the level of ammonia reached a toxic concentration of 2.0-3.6 mmol/l. Since these cell lines utilized only a part of carbon and nitrogen sources in the medium, it was suggested that the final cell density was limited by a growth inhibitor (i.e. ammonia) and not by a lack of nutrients.
Asunto(s)
Leucemia/metabolismo , Células Tumorales Cultivadas/metabolismo , Aminoácidos/metabolismo , Amoníaco/análisis , Recuento de Células , División Celular , Línea Celular , Medios de Cultivo/análisis , Glucosa/metabolismo , Glutamina/metabolismo , Humanos , Cetoácidos/análisis , Leucemia/patología , Ornitina/análisis , Piruvatos/análisisRESUMEN
A double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed for measuring Aujeszky's disease virus (ADV) antigen concentration and an inhibition technique based on the former was developed for detection of antibodies to ADV. The results were checked by determining the cytopathic and serum neutralization titres. The correlation was satisfactory in both cases, with correlation coefficients above 0.8. When measuring ADV antigen concentration, the lower limit of detection was 10(3) TCID 50/0.2 ml. The sensitivity of ELISA in detecting antibodies to ADV was found to be superior to that of the serum neutralization test and, thus, enabled the testing of rabbit and guinea-pig sera.