RESUMEN
BACKGROUND: Large student numbers and heterogeneous teaching pools hamper standardized teaching and impede objective assessment of surgical skills. This article presents the advantages of new teaching media in a "blended learning" concept for training surgical skills to medical students at the Basel University Medical School in Switzerland. MATERIAL AND METHODS: The surgical skills course (suture course) for medical students was redesigned according to a blended learning concept consisting of an introduction with a multimedia CD-ROM, a practical course, and a skills lab. The learning targets of the course were evaluated through an objective structured clinical examination (OSCE) at the end of each study year. The students' own course evaluations were compared with the OSCE results before and after introduction of the new blended learning. RESULTS: The students' evaluations with regard to teaching material, subjective practical achievement, prospective value for the practical year, and overall course evaluation were significantly higher than in the old course format. The proportion of passed OSCEs was 10% higher after the redesign of the course. CONCLUSION: Blended learning can improve cognition and performance as well as the training efficiency and duration required for mentoring. Thus human resources can be saved indirectly. Surgical procedures may be presented more clearly.
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Competencia Clínica/normas , Instrucción por Computador/métodos , Procedimientos Quirúrgicos Operativos/educación , Actitud del Personal de Salud , Discos Compactos , Instrucción por Computador/normas , Curriculum/normas , Humanos , Motivación , Multimedia , Evaluación de Programas y Proyectos de Salud , Consejos de Especialidades , Procedimientos Quirúrgicos Operativos/normas , Técnicas de Sutura/educación , Técnicas de Sutura/normas , SuizaRESUMEN
Accuracy of antigen determination in human plasma samples is often adversely affected by immune complex formation between antigens (e.g., HIV-1 p24 protein) and specific antibodies. In this study we describe an optimized method for complete immune complex dissociation (ICD) in plasma. This method is based on heat denaturation of antibodies and utilizes a defined solution of sodium dodecyl sulfate (SDS) and diethylenetriaminepentaacetic acid (DTPA) as diluent. The efficiency of this procedure for ICD was compared with those of published methods, employing heat denaturation alone and acidification. Plasma samples from patients participating in anti-retroviral treatments and samples reconstituted in vitro were treated and analyzed in parallel. HIV-1 p24 antigen was determined by quantitative enzyme-linked immunosorbent assay (ELISA). In 312 samples from 97 patients, antigenemia was found in 44.9% when measured directly and in 87.2% after this treatment. In a subset of 56 samples, 21.4% tested positive prior to treatment, while after either novel treatment, heat denaturation or acidification, these samples tested positive in 80.4%, 62.5% and 60.7%, respectively. In 94% of cases viral RNA was detected. This improved procedure for ICD provides a reliable and convenient method for complete and accurate p24 antigen detection in human plasma and is applicable to commercially available test kits.
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Complejo Antígeno-Anticuerpo/química , Proteína p24 del Núcleo del VIH/aislamiento & purificación , Infecciones por VIH/virología , VIH-1/aislamiento & purificación , Carga Viral , Viremia/virología , Complejo Antígeno-Anticuerpo/sangre , Ensayo de Inmunoadsorción Enzimática , Anticuerpos Anti-VIH/inmunología , Proteína p24 del Núcleo del VIH/sangre , Proteína p24 del Núcleo del VIH/inmunología , Infecciones por VIH/sangre , VIH-1/genética , VIH-1/inmunología , Calor , Humanos , Ácido Pentético , Plasma , Desnaturalización Proteica , ARN Viral/sangre , Juego de Reactivos para Diagnóstico , Reproducibilidad de los Resultados , Dodecil Sulfato de SodioRESUMEN
Rapid diagnosis of herpes simplex encephalitis (HSE) can only be achieved by the polymerase chain reaction (PCR). In order to carry out PCR under routine conditions, it is of great importance to establish an easy DNA extraction protocol and especially a rapid and sensitive DNA detection method. In the present study, two different solid phase hybridization assays (Gen-Eti-K-DNA Enzyme Immunoassay (DEIA), Sorin Biomedica, Italy and Enzymun-Test DNA detection, Boehringer Mannheim, Germany) were compared for detection of PCR amplified HSV DNA polymerase genome region, using standard primers, from cerebrospinal fluid (CSF) samples. 122 CSF samples obtained from patients suffering from encephalitis and hospitalized at the University Clinics of Frankfurt and Graz during the period January 1992 to July 1993 were tested. To ascertain the sensitivity of the hybridization assays, dilution series of a plasmid, encoding the amplified region of the polymerase gene, were investigated. The detection limit of the DEIA assay was one copy of the plasmid/microliter, and the lowest amount of DNA which could be detected by the Enzymun assay as well as Southern blot was 10 copies/microliter. 15 CSF samples obtained from patients with HSE were found positive by the three assays. Concordant results were also obtained with CSF samples from non-HSE patients. The results of this study show that new hybridization systems guarantee a fast and high-sensitive detection of amplified HSV DNA. HSV PCR in CSF can be carried out routinely by the combined use of rapid hybridization and a simple extraction procedure.
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Encefalitis Viral/virología , Reacción en Cadena de la Polimerasa/métodos , Simplexvirus/genética , Secuencia de Bases , ADN Complementario , Encefalitis Viral/líquido cefalorraquídeo , Genoma Viral , Humanos , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Simplexvirus/aislamiento & purificaciónRESUMEN
The neurological manifestations of Lyme borreliosis comprise a wide range of clinical signs. However, these symptoms might have other aetiologies. Therefore detection of intrathecal production of specific antibodies is necessary to confirm the clinical assumption of neuroborreliosis (NB). In case of delayed intrathecal production of specific IgG antibodies, detection of IgM could play a role in the early diagnosis of NB. To clarify whether IgM is of diagnostic value in such cases, paired CSF serum samples from 176 patients with suspected NB admitted to the department of Neurology, Karl Franzens University, Graz, Austria, were tested. Testing was performed with the IDEA Neuroborreliosis Kit (Dako, Denmark) and Enzygnost Borreliosis (Behring, Germany) and results of both methods were compared. According to well defined criteria 63 of the 176 patients had defined NB and 113 were regarded as possible NB. Twelve out of 63 patients with defined NB had delayed intrathecal IgG production. Only one patient with delayed IgG production had an intrathecal IgM production prior to IgG. In all patients with possible NB no intrathecal production of IgM was detected. At the time of the first lumbar puncture IgG intrathecal production could be detected with the IDEA seven times more often than with the Enzygnost Borreliosis. The determination of intrathecal production of IgM does not appear to be of diagnostic value in patients with delayed IgG antibody production. Therefore a consecutive lumbar puncture is more likely to confirm clinical assumption if there is strong clinical evidence of NB.
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Anticuerpos Antibacterianos/líquido cefalorraquídeo , Grupo Borrelia Burgdorferi/inmunología , Inmunoglobulina G/líquido cefalorraquídeo , Inmunoglobulina M/líquido cefalorraquídeo , Enfermedad de Lyme/diagnóstico , Meningoencefalitis/diagnóstico , Polineuropatías/diagnóstico , Adulto , Ensayo de Inmunoadsorción Enzimática , Femenino , Estudios de Seguimiento , Humanos , Enfermedad de Lyme/inmunología , Masculino , Meningoencefalitis/inmunología , Polineuropatías/inmunología , Valor Predictivo de las PruebasRESUMEN
Seventy-one isolates of Borrelia burgdorferi sensu lato (B.b.s.l.) derived from Ixodes ricinus ticks (50 strains) and patients (21 strains) were characterised by PCR-RLFP analysis. In four cases the human isolates were obtained from the cerebrospinal fluid (CSF) of patients with clinical symptoms of neuroborreliosis and in 17 cases from skin biopsies of patients with dermatological manifestation of Lyme borreliosis. Ixodes ricinus isolates originated from 14 localities in three regions (Mur valley, eastern and western Styria) in Styria. Thirty six strains of B.b.s.l. were isolated from nymphal ticks, nine strains from female and five strains from male ticks. Species identification of human isolates revealed three B. garinii and one B. afzelii isolates in CSF. In the PCR-RFLP analysis of 17 skin specimens a pattern for B. afzelii was found in ten cases, while six could be identified as B. garinii and one as a mixed infection of B. afzelii and B. garinii. Genetic characterisation of tick isolates resulted in 24 strains of B. afzelii (48%), 11 strains of B. garinii (40%) and 5 strains of B. burgdorferi s.st. (10%); one isolate showed a mixed infection of B. afzelii and B. garinii. Our findings indicate that B. afzelii and B. garinii predominate over B. burgdorferi s.str. in Ixodes ricinus ticks from Styria, which is similar to findings in neighbouring countries. This also reflects the occurrence of different pathogenic Borrelia strains in human samples.
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Grupo Borrelia Burgdorferi/genética , Borrelia/genética , Ixodes/microbiología , Enfermedad de Lyme/microbiología , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Animales , Austria , Borrelia/clasificación , Borrelia/aislamiento & purificación , Grupo Borrelia Burgdorferi/clasificación , Grupo Borrelia Burgdorferi/aislamiento & purificación , Femenino , Humanos , Enfermedad de Lyme/diagnóstico , Masculino , Especificidad de la EspecieRESUMEN
Affections of the major salivary glands are relatively rare in stomatology; but according to the indications of several authors, the proportion of sialolithiasis to the total amount of affections of the salivary glands (ranging from 20.5 to 55.5%) is great. The concretions are, as a rule, located in the duct system of the submandibular gland where they may reach, circumstances permitting, considerable sizes. The author's own investigations evidence that these calculi have a partly regular, partly irregular shell-like structure around a non-calcified centre. Chemical analysis by means of X-ray diffractometry revealed hydroxyapatite varying in the degree of cristallinity as the major component. Precursors in the form of octacalcium phosphate and dicalcium phosphate dihydrate have been disclosed. Salivary calculi must be removed (most frequently by surgical intervention) to avoid secondary diseases of the duct system of the respective gland.
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Cálculos del Conducto Salival/diagnóstico , Cristalización , Humanos , Cálculos del Conducto Salival/patología , Cálculos del Conducto Salival/cirugía , Difracción de Rayos XRESUMEN
117 cases of clinically manifest tick-borne encephalitis (TBE) in Styria, Austria, the years 1987 to 1990 are reported in terms relevant anamnestic data, clinical findings (meningitis, meningoencephalitis, meningoradiculitis) and course. The geographic distribution corresponds to the known endemic areas for TBE in Styria. We found a significant decrease of incidence. The prognosis of the disease was benign in general; more than 80% ran a course free from complications. A small proportion (5%), however, suffer from severe residual handicap. These patients are subsumed under the group of meningo-radiculitides. Two patients (1.8%) acquired the disease despite having been vaccinated regularly.
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Encefalitis Transmitida por Garrapatas/diagnóstico , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Anticuerpos Antivirales/líquido cefalorraquídeo , Austria/epidemiología , Estudios Transversales , Virus de la Encefalitis Transmitidos por Garrapatas/inmunología , Encefalitis Transmitida por Garrapatas/epidemiología , Femenino , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Examen NeurológicoRESUMEN
A total of 1163 I. ricinus ticks were collected in 3 different regions (15 localities) in Styria (Austria) in June 1997 and examined for the presence of spirochetes by dark field microscopy. The mean infection rate was 20.8%. Among 310 adults, 24.2% were positive and among 853 nymphs, 19.6% were positive. All 15 collection areas were shown to harbour infected nymphs with a positivity rate ranging from 5.8% (3/52) to 32.1% (18/56). Isolation attempts in BSKII medium resulted in 29 isolates. Species identification by PCR-RFLP analysis revealed 16 strains of B. garinii, 10 strains of B. afzelii and 2 strains of B. burgdorferi s. s. One isolate showed a mixed population of B. garinii and B. afzelii. In two collection areas, all three major Borrelia species were shown to be present in the tick population.
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Vectores Arácnidos/microbiología , Grupo Borrelia Burgdorferi/aislamiento & purificación , Ixodes/microbiología , Reacción en Cadena de la Polimerasa/métodos , Animales , Austria , Técnicas de Tipificación Bacteriana , Grupo Borrelia Burgdorferi/clasificación , Grupo Borrelia Burgdorferi/genética , Femenino , Genes de ARNr , Ixodes/crecimiento & desarrollo , Masculino , Ninfa/microbiología , Polimorfismo de Longitud del Fragmento de Restricción , ARN Ribosómico 23S/genética , ARN Ribosómico 5S/genéticaRESUMEN
Polymerase chain reaction-based molecular assays are gaining increasing importance in the diagnosis and monitoring of infectious diseases. Over the past several years, the development and application of these techniques has initiated a revolution in the diagnosis and monitoring of hepatitis C infection. Presently, molecular assays are exclusively done in especially dedicated laboratories. Advances in automation will bring these technologies into routine diagnostic laboratories and will make them widely used.
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Hepacivirus/aislamiento & purificación , ARN Viral/sangre , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Automatización , Hepacivirus/genética , HumanosRESUMEN
The use of AMPLILINK version 1.0 software was evaluated for the operation and control of one COBAS AMPLICOR instrument and for two COBAS AMPLICOR instruments run simultaneously to perform and detect nucleic acid amplification reactions. A total of 3,384 results were analyzed. The initial accuracy of the results was 99.91%. Three errors of omission of transfer of data from the COBAS AMPLICOR to the AMPLILINK system were observed. Two of these errors were from a single specimen, where both the analyte and internal control results were not transmitted. These errors did not interfere with the correctness of any other data. There were no interruptions of runs, and no data were mixed. AMPLILINK increased convenience, saved labor, and was found to be a very useful addition for clinical laboratories performing molecular-diagnostic procedures with the COBAS AMPLICOR system.
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Reacción en Cadena de la Polimerasa/métodos , Programas Informáticos , Estudios de Evaluación como Asunto , Interfaz Usuario-ComputadorRESUMEN
Lyme borreliosis and tick-borne encephalitis (TBE) are the most common diseases in Austria caused by tick bites. TBE endemic areas are well defined. It seemed to be of interest to compare prevalence data of antibodies against Borrelia burgdorferi (B.b.) to TBE endemic and non endemic areas. Blood samples (n = 1162) were obtained from healthy blood donors in combination with a standardized questionnaire during 21 excursions to 7 selected regions of Styria, Austria. Serum samples were screened for IgG antibodies against B.b. by a commercial flagellum ELISA. None of the tested persons showed symptoms of active Lyme borreliosis. A higher prevalence of antibodies against B.b. could be found in TBE endemic areas (7.7%) compared to TBE nonendemic areas (3.8%). There was a significant increase in positive antibodies against B.b. with age, exposure and number of tick bites remembered by test persons. The antibody prevalence to B.b. flagellin antigen is significantly higher in TBE endemic areas than in non-endemic comparative regions.
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Anticuerpos Antibacterianos/sangre , Donantes de Sangre , Grupo Borrelia Burgdorferi/inmunología , Flagelina/inmunología , Enfermedad de Lyme/epidemiología , Adolescente , Adulto , Anciano , Austria/epidemiología , Femenino , Humanos , Enfermedad de Lyme/inmunología , Enfermedad de Lyme/microbiología , Masculino , Persona de Mediana Edad , Prevalencia , Estudios SeroepidemiológicosRESUMEN
GOAL OF THIS STUDY: The Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis (Roche Molecular Systems, Branchburg, NJ) was evaluated on conjunctival, pharyngeal, and urethral swabs. STUDY DESIGN: A total of 515 conjunctival, pharyngeal, and urethral swabs. The reference system was culture with McCoy cells in shell vials with fluorescent immunostaining. One swab was used for both cell culture and the molecular assay. Initial storage took place in 2-SP medium. After transfer to Amplicor specimen transport medium the molecular assay was done using the Amplicor Chlamydia trachomatis amplification and detection kits. RESULTS: The total positive rate was 6.6%. Specificity of culture was 100%. The evaluated molecular assay gave a specificity of 99.8%. Sensitivities of PCR and culture were 100% and 85.3%, respectively. CONCLUSIONS: Because of the high sensitivity, specificity, and ease of use, the molecular assay was found to be a good alternative to culture for detection of C. trachomatis in conjunctival, pharyngeal, and urethral specimens.
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Infecciones por Chlamydia/diagnóstico , Chlamydia trachomatis , Enfermedades Urogenitales Femeninas/diagnóstico , Enfermedades Urogenitales Masculinas , Reacción en Cadena de la Polimerasa/métodos , Adolescente , Adulto , Anciano , Infecciones por Chlamydia/epidemiología , Infecciones por Chlamydia/microbiología , Infecciones por Chlamydia/orina , Femenino , Enfermedades Urogenitales Femeninas/epidemiología , Enfermedades Urogenitales Femeninas/microbiología , Enfermedades Urogenitales Femeninas/orina , Humanos , Masculino , Persona de Mediana Edad , Prevalencia , Sensibilidad y EspecificidadRESUMEN
OBJECTIVES: The antibody response in sera and tears of 167 patients with suspected chlamydial conjunctivitis was compared with the antibody response in sera and tears of 45 patients with symptoms of urogenital chlamydial infection to discover whether and which type of antichlamydial antibody detected in tears may be of diagnostic help in chlamydial conjunctivitis. METHODS: Diagnosis was based on chlamydial antigen detection from the conjunctiva and urogenital tract, done by a direct immunofluorescence assay, McCoy cell culture, and polymerase chain reaction. Additionally, antichlamydial immunoglobulin A (IgA) and immunoglobulin G (IgG) were determined in sera and tears of all patients by an immunoperoxidase assay. RESULTS: Two hundred twelve patients were examined--167 with conjunctivitis, 45 with symptoms of urogenital chlamydial infection. Cell culture, direct immunofluorescence assay, and polymerase chain reaction brought identical results. Conjunctival specimens taken from 33 (20%) of the patients with conjunctivitis were Chlamydia antigen positive; specimens taken from 134 (80%) were negative. Antichlamydial antibodies were found in tears of 29 (88%) of the patients with conjunctivitis whose specimens were Chlamydia antigen positive. Fifty-four (40%) of the patients with conjunctivitis whose specimens were Chlamydia antigen negative had antichlamydial antibodies in their tears. Twenty-five patients with urethritis (56%) were Chlamydia antigen positive in urethral swabs; 20 (44%) were negative. Antichlamydial antibodies were found in the tears of eight (32%) of the Chlamydia antigen-positive and two (10%) of the Chlamydia antigen-negative patients with urethritis. In contrast to patients with conjunctivitis, findings for patients with urethritis always were negative for antichlamydial IgG in the tears. CONCLUSION: Antichlamydial antibodies in tears were seen significantly more often in patients with conjunctivitis than in those with urethritis (P < or = 0.05). Antichlamydial IgG was found only in tears of patients with conjunctivitis. Therefore, the authors conclude that the detection of antichlamydial IgG in the tears might be helpful for diagnosis in patients with suspected chlamydial conjunctivitis who have antigen-negative conjunctival swabs.
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Anticuerpos Antibacterianos/análisis , Chlamydia trachomatis/inmunología , Conjuntivitis de Inclusión/diagnóstico , Lágrimas/inmunología , Adolescente , Adulto , Anciano , Antígenos Bacterianos/inmunología , Niño , Infecciones por Chlamydia/diagnóstico , Infecciones por Chlamydia/inmunología , Chlamydia trachomatis/genética , Chlamydia trachomatis/aislamiento & purificación , Conjuntiva/inmunología , Conjuntiva/microbiología , Conjuntivitis de Inclusión/inmunología , ADN Bacteriano/análisis , Femenino , Técnica del Anticuerpo Fluorescente Directa , Humanos , Inmunoglobulina A/análisis , Inmunoglobulina A Secretora/análisis , Inmunoglobulina G/análisis , Masculino , Técnicas Microbiológicas , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa/métodos , Uretritis/inmunología , Uretritis/microbiologíaRESUMEN
A molecular assay based on a rapid DNA extraction protocol and the EnviroAmp Legionella Kits was used to detect Legionella species in bronchoalveolar fluid specimens. All Legionella strains isolated from tap water in hospitals could be detected distinctly. Both sensitivity and specificity were tested. In a prospective study, bronchoalveolar lavage fluids obtained from patients with atypical pneumonia were investigated. Three positive samples were detected with the molecular techniques and were subsequently confirmed by culture. Application of the system described may lead to safe and early diagnosis of Legionnaires' disease in patients with atypical pneumonia.
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Líquido del Lavado Bronquioalveolar/microbiología , Legionella/genética , Legionella/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Técnicas Bacteriológicas/estadística & datos numéricos , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Estudios de Evaluación como Asunto , Humanos , Legionella pneumophila/genética , Legionella pneumophila/aislamiento & purificación , Legionelosis/diagnóstico , Enfermedad de los Legionarios/diagnóstico , Reacción en Cadena de la Polimerasa/estadística & datos numéricos , Sensibilidad y Especificidad , Especificidad de la Especie , Factores de TiempoRESUMEN
A molecular assay for the detection of herpes simplex virus (HSV), including a novel, nonradioactive hybridization technique, was evaluated with a total of 123 cerebrospinal fluid specimens. After DNA extraction, specific HSV DNA sequences were amplified with digoxigenin-labeled primers derived from the DNA polymerase gene-coding region from HSV. Amplified products were detected by the Enzymun-Test DNA detection assay (Boehringer, Mannheim, Federal Republic of Germany), which uses biotinylated probes. Amplification with nonlabeled primers and then Southern blotting and nonradioactive detection of hybrids by the digoxigenin technique was the reference system. The sensitivities of the molecular assays were determined with 10-fold dilutions of plasmid pS4 with the SalI restriction fragment of the DNA polymerase gene obtained from the HSV type 1 strain Angelotti. The Enzymun assay was able to detect all of the 16 positive samples, giving 100% agreement with the Southern blot hybridization results. Optical density values were widely separated for the positive and negative groups of specimens. Ten copies of plasmid pS4 per microliter could be distinctly detected by the Enzymun assay. The cutoff was determined for the hybridization assay, and an equivocal zone was defined. The whole molecular assay including the Enzymun-Test DNA detection proved to be sensitive and easy to use. It may contribute to the rapid and safe detection of HSV DNA in cerebrospinal fluid.
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ADN Viral/líquido cefalorraquídeo , ADN Polimerasa Dirigida por ADN/genética , Herpes Simple/microbiología , Reacción en Cadena de la Polimerasa/métodos , Artefactos , Secuencia de Bases , Southern Blotting , Cartilla de ADN , ADN Viral/aislamiento & purificación , Digoxigenina , Herpes Simple/enzimología , Humanos , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
BACKGROUND: Diagnosis of chlamydial conjuctivitis is difficult in chronic diseases because chlamydial elementary bodies are mostly undetectable in conjunctival scrapings by cell culture. We therefore compared two nonculture antigen tests and three different serotests for anti-chlamydial antibodies with McCoy cell culture, the "gold standard" of chlamydial diagnosis. Conjunctival scrapings and serum samples of 93 patients attending the outpatient eye clinic in Graz because of chronic follicular conjunctivitis were tested. METHODS: A total of 558 conjunctival scrapings and 93 serum samples were investigated. Chlamydial antigen detection was done by McCoy cell culture, polymerase chain reaction (PCR; Amplicor, Roche), and direct immunofluorescence assay (DFA; Microtrak, Syva). Antichlamydial IgA and IgG antibodies in the sera were detected by an immunoperoxidase assay (IPAzyme, Savyon) and two different enzyme-linked immunosorbent assays (SeroELISA, Savyon and rELISA, medac). RESULTS: Cell culture and PCR yielded identical results. The positivity rate for chlamydial conjunctivitis was 8.6% (8 of 93 patients). PCR proved most sensitive and most specific. IPAzyme was 75% sensitive for IgA and 100% for IgG; SeroELISA and rELISA were less sensitive. IPAzyme was 81% specific for IgA and 47.3% for IgG. SeroELISA and rELISA were less specific for IgA, but more specific for IgG. Post-test likelihood of disease was greatest in IPAzyme. CONCLUSIONS: PCR proved to be a good alternative to cell culture; DFA is useful for quick diagnosis. Genus-specific serotests cannot compete with chlamydial antigen detection. They differ in sensitivity and specificity because of the antigen type they present. They are still of only supportive value in cases where chlamydial antigen detection is not possible. Recently introduced species-specific antibody tests should be of greater value.
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Anticuerpos Antibacterianos/análisis , Antígenos Bacterianos/análisis , Chlamydia trachomatis/inmunología , Conjuntivitis de Inclusión/diagnóstico , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Chlamydia trachomatis/genética , ADN Bacteriano/análisis , Ensayo de Inmunoadsorción Enzimática/métodos , Estudios de Evaluación como Asunto , Femenino , Técnica del Anticuerpo Fluorescente Directa/métodos , Humanos , Técnicas para Inmunoenzimas , Inmunoglobulina A/análisis , Inmunoglobulina G/análisis , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa/métodos , Sensibilidad y Especificidad , Pruebas Serológicas/métodosRESUMEN
The Amplicor HBV Monitor Test for quantitative detection of serum hepatitis B virus (HBV) DNA has recently been introduced. This assay is based on PCR and a non-radioactive hybridization and detection system on microwell plates. Evaluation in a routine diagnostic laboratory showed excellent sensitivity and adequate reproducibility; however, a more automated format would be desirable. The Amplicor HBV Monitor Test is useful for recognizing those patients who might benefit from antiviral treatment and for evaluation of the efficacy of anti-hepatitis B virus treatment.
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ADN Viral/sangre , Virus de la Hepatitis B/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Virus de la Hepatitis B/genética , Humanos , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
It is suggested that Borrelia burgdorferi infection could be associated with dilated cardiomyopathy (IDC). Stanek et al. were able to cultivate Borrelia burgdorferi from myocardial biopsy tissue of a patient with longstanding dilated cardiomyopathy. Here we present a study in which we examined the effect of standard antibiotic treatment on the left ventricular ejection fraction (LVEF) in patients with dilated cardiomyopathy associated with Borrelia burgdorferi infection. In this study we assessed the serum (IgG, IgM Elisa) and history of 46 IDC patients with specific regard to Borrelia burgdorferi infection (mean LVEF 30.4 +/- 1.3%, measured by cardiac catheterization and echocardiography with the length-area-volume method). All 46 patients received standard treatment for dilated cardiomyopathy: ACE inhibitors, digitalis, and diuretics. Eleven (24%) patients showed positive serology and a history of Borrelia burgdorferi infection; nine of these also had a typical history of tick bite and erythema chronicum migrans (ECM) and/or other organ involvement, and two had no recollection of tick bite or ECM but showed other Borrelia burgdorferi-associated disorders (neuropathy, oligoarthritis). These 11 patients with Borrelia burgdorferi infection received standard antibiotic treatment with intravenous ceftriaxone 2 g bid for 14 days. Six (55%) recovered completely and showed a normal LVEF after 6 months, three (27%) improved their LVEF, and two (18%) did not improve at all. This amounts to nine (82%) patients with recovery/improvement in the Borrelia burgdorferi group. The 35 patients who did not show positive serology or a history of Borrelia burgdorferi infection did not receive antibiotic treatment. In this group without Borrelia burgdorferi infection 12 (26%), showed recovery/improvement following the standard treatment of dilated cardiomyopathy (see earlier). Our results indicate that Borrelia burgdorferi infection could play a decisive role in the development of dilated cardiomyopathy, especially in a geographical region such as Graz, where Borrelia burgdorferi is endemic. While we are aware of the small number of Borrelia burgdorferi patients in this study, we nevertheless conclude that in a remarkable number of patients with signs of Borrelia burgdorferi infection, dilated cardiomyopathy could be reversed and LVEF improved.
Asunto(s)
Infecciones por Borrelia/tratamiento farmacológico , Grupo Borrelia Burgdorferi/efectos de los fármacos , Cardiomiopatía Dilatada/tratamiento farmacológico , Ceftriaxona/uso terapéutico , Cefalosporinas/uso terapéutico , Volumen Sistólico/efectos de los fármacos , Adolescente , Adulto , Anciano , Inhibidores de la Enzima Convertidora de Angiotensina/administración & dosificación , Inhibidores de la Enzima Convertidora de Angiotensina/uso terapéutico , Infecciones por Borrelia/diagnóstico , Infecciones por Borrelia/fisiopatología , Cardiomiopatía Dilatada/diagnóstico , Cardiomiopatía Dilatada/epidemiología , Cardiomiopatía Dilatada/microbiología , Ceftriaxona/administración & dosificación , Ceftriaxona/efectos adversos , Ceftriaxona/farmacología , Cefalosporinas/administración & dosificación , Cefalosporinas/farmacología , Distribución de Chi-Cuadrado , Medios de Cultivo , Digitalis , Diuréticos/administración & dosificación , Diuréticos/uso terapéutico , Electrocardiografía , Femenino , Corazón/microbiología , Humanos , Inmunoglobulina G/sangre , Inyecciones Intravenosas , Masculino , Persona de Mediana Edad , Plantas Medicinales , Plantas TóxicasRESUMEN
BACKGROUND: The COBAS AMPLICOR (CA) instrument for the amplification and detection steps of the AMPLICOR molecular diagnostic assays has recently been introduced. The system contains a single thermal cycler with two independently controlled heating/cooling blocks, a pipettor, a magnetic particle washer, a photometer and an incubator. OBJECTIVE: The performance of the CA instrument was evaluated in a routine diagnostic laboratory for the detection of hepatitis C virus (HCV) RNA. The new system was compared with the corresponding microwell plate assay (AMPLICOR HCV Test). STUDY DESIGN: Routine clinical sera (350) from hemodialysis patients and patients with chronic HCV infection and interferon therapy were studied. If discrepant results were obtained, both assays were repeated (specimen preparation, amplification and detection); in addition, the HCV copy number was determined with the AMPLICOR HCV MONITOR Test. RESULTS: There was a correlation between the CA HCV Test and the AMPLICOR HCV Test in 341 of 350 specimens (97%). After resolution of 9 discrepant results, the CA HCV Test gave a sensitivity of 97.8% and a specificity of 99.4%. The most common reason for discrepant results was a low HCV RNA copy number. CONCLUSION: The CA system was found to be a labor-saving, fast and reliable instrument for the amplification and detection steps of a RT-PCR molecular assay for detection of HCV RNA.
Asunto(s)
Hepacivirus/química , Hepacivirus/genética , Reacción en Cadena de la Polimerasa/instrumentación , ARN Viral/sangre , Infecciones por Flaviviridae/diagnóstico , Infecciones por Flaviviridae/genética , Humanos , ARN Viral/genética , Sensibilidad y EspecificidadRESUMEN
BACKGROUND: Hepatitis C virus (HCV) infection is highly prevalent in hemodialysis and AIDS patients. Little information exists about the viral load in those patients. OBJECTIVE: To characterize HCV infection in hemodialysis and AIDS patients, the viral load in the sera was measured. Results were compared with genotypes, gender of the patients, and biochemical markers of active hepatitis. STUDY DESIGN: Sera from a total of 442 patients were screened with a third-generation EIA, and anti-HCV immunoreactivity was confirmed with the Wellcozyme HCV Western Blot. After qualitative PCR with the Amplicor PCR Test, positives were genotyped using a reverse hybridization test. Determination of HCV levels was done with the Amplicor HCV Monitor assay. RESULTS: HCV RNA was detected in the sera of 95 (74.8%) EIA-positive patients. HCV RNA levels ranged from 1 x 10(4) to 1.4 x 10(6) molecules of HCV RNA/ml. Median HCV RNA levels of AIDS patients were slightly higher than those of hemodialysis patients. Male patients had higher median HCV RNA levels compared with female patients. No association between HCV RNA levels and both alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels was found. The most common genotypes were type 1b and type 1a, followed by type 3, type 4, and type 2a. There were no significant differences in HCV RNA levels among patients with genotypes 1a, 1b, and 2a. Patients infected with types 3 and 4, respectively, had significantly lower HCV RNA levels compared with other genotypes. CONCLUSION: Because the Amplicor HCV Monitor assay allows quantitation of low-titer viremic patients, HCV RNA levels were distinctly lower compared with previous reports. HCV RNA levels of males did not differ significantly from those of females. ALT and AST are very poor indicators of ongoing HCV infection. Patients with chronic type 3 or type 4 HCV infection tended to have lower HCV RNA levels.