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1.
Int Tinnitus J ; 17(1): 21-5, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23906823

RESUMEN

INTRODUCTION: The perception of tinnitus varies among individuals. The limitations caused by tinnitus are related to psychological factors, mood changes and psychiatric conditions, while other factors related to discomfort caused by tinnitus are being studied. Hearing loss is an important factor for the onset of tinnitus. OBJECTIVES: To evaluate the correlation between the degree of discomfort caused by tinnitus and the hearing loss level. MATERIALS AND METHODS: A retrospective study of the patients treated at the Otolaryngology Service of the State University from Campinas for 15 months, using the Visual-Analogue Scale to classify the degree of discomfort by tinnitus. RESULTS: 107 patients were studied and there was no correlation between the degree of annoyance of tinnitus with hearing loss, age, gender, presence of dizziness, of neck pain, headache, changes of the temporomandibular joint, the use of caffeine or excessive intake of carbohydrates. The discomfort was slightly higher in patients without hearing loss and in women. CONCLUSION: Dizziness, neck pain, headache and caffeine abuse are prevalent complaints in patients with tinnitus.


Asunto(s)
Pérdida Auditiva , Acúfeno , Mareo , Cefalea , Humanos , Estudios Retrospectivos , Acúfeno/psicología
2.
J Invest Surg ; 20(1): 41-8, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17365406

RESUMEN

After total gastrectomy, the ileocecal graft may act as a reservoir and protect against reflux but give rise to transposition of the ileum and cause possible changes in bile acid metabolism and nutrition. This study compared the ileocecal graft and jejunal pouch. Male Wistar rats weighing 265 +/- 22 g were submitted to sham operation (S), ileocecal interposition graft (IIG), and jejunal pouch interposition graft (JP) after total gastrectomy. Eight weeks later, the esophagus was examined for evidence of esophagitis. Nutritional biochemistry and weight profile were documented preoperatively and 8 weeks after surgery. The oral glucose tolerance test was performed. Thirty-three rats were operated on and 30 survived for 8 weeks. Esophagitis occurred in seven JP rats. Body weight was significantly higher in IIG than in JP rats (p < .05). Normal glucose tolerance to intragastric glucose load was observed in sham and operated rats. JP rats had a significant decrease in serum albumin, glucose, transferrin, hemoglobin, iron, folate, and calcium, compared to sham (p < .05). Cobalamine was significantly lower in IIG rats than in JP rats (p < .05). In the IIG and JP groups, serum/hepatic total bile acid did not differ significantly from preoperative and sham values. In conclusion, the IIG interposition graft in rats prevented esophagitis, preserved nutrition, and did not interfere with enterohepatic total bile acid circulation.


Asunto(s)
Gastrectomía/métodos , Válvula Ileocecal/trasplante , Yeyuno/trasplante , Síndromes Posgastrectomía/prevención & control , Trasplante Heterotópico , Anastomosis Quirúrgica , Animales , Ácidos y Sales Biliares/metabolismo , Peso Corporal , Calcio/sangre , Duodeno/cirugía , Circulación Enterohepática , Esofagitis Péptica/prevención & control , Esófago/cirugía , Ácido Fólico/sangre , Reflujo Gastroesofágico/prevención & control , Prueba de Tolerancia a la Glucosa , Hemoglobinas/análisis , Hipocalcemia/etiología , Absorción Intestinal , Lípidos/sangre , Masculino , Complicaciones Posoperatorias/prevención & control , Ratas , Ratas Wistar , Transferrina/análisis , Vitamina B 12/sangre
3.
J Invest Surg ; 19(1): 39-46, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16546928

RESUMEN

Intestinal ischemia/reperfusion (I/R) may induce bacterial translocation (BT). Glutamine (GLN)-enriched nutrition decreases BT. However, little is known about the effect of glucan (GL) in BT. This study investigated the combined effect of GL/GLN on BT, intestinal damage, and portal blood cytokines in animals under I/R. Four groups of 10 rats each were subjected to 60 min of intestinal ischemia and 120 min of reperfusion. The control group (group 1) received only rat food/water, group 2 received glutamine via gavage, group 3 received subcutaneuos soluble (1, 3)-d-glucan, and group 4 received GL + GLN. A sham group (group 5) served as a normal control. Bacterial cultures of ileum, mesenteric lymph nodes (MLN), liver and lung biopsies, histological changes of ileum, and serum cytokines variables were examined after I/R. Data were analyzed by analysis of variance (ANOVA) and the Newman-Keuls test. Results showed that GLN, GL, and GL/GLN significantly reduced BT to MLN, liver, and lung. BT was more attenuated after GL treatment than GLN (P < .05). Rats treated with both GL and GLN exhibited lower bacterial colony counts than the ones treated only with GLN or GL. Severe mucosal damage on histological findings was shown in group 1, but these findings were significantly ameliorated (P < .05) in groups 3 and 4. Tumor necrosis factor (TNF)-a and interleukin (IL)-6 levels in portal serum were significantly reduced and IL-10 was increased by GL and GLN treatment. In conclusion, the use of GL was more effective than GLN in reducing BT, intestinal damage, and cytokine levels after I/R. Additionally, the combination of GL and GLN improved results.


Asunto(s)
Glucanos/farmacología , Glutamina/farmacología , Íleon/irrigación sanguínea , Íleon/lesiones , Daño por Reperfusión/tratamiento farmacológico , Daño por Reperfusión/microbiología , Animales , Citocinas/sangre , Enterobacteriaceae/aislamiento & purificación , Íleon/efectos de los fármacos , Íleon/microbiología , Masculino , Ratas , Ratas Wistar , Daño por Reperfusión/patología
4.
Arq Gastroenterol ; 43(4): 288-92, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-17406757

RESUMEN

BACKGROUND: [corrected] There is substantial evidence that infection with Helicobacter pylori plays a role in the development of gastric cancer and that it is rarely found in gastric biopsy of atrophic gastritis and gastric cancer. On advanced gastric tumors, the bacteria can be lost from the stomach. AIMS: To analyze the hypothesis that the prevalence of H.pylori in operated advanced gastric carcinomas and adjacent non-tumor tissues is high, comparing intestinal and diffuse tumors according to Lauren's classification METHODS: A prospective controlled study enrolled 56 patients from "Hospital Universitário", Federal University of Rio Grande do Norte, Natal, RN, Brazil, with advanced gastric cancer, treated from February 2000 to March 2003. Immediately after partial gastrectomy, the resected stomach was opened and several mucosal biopsy samples were taken from the gastric tumor and from the adjacent mucosa within 4 cm distance from the tumor margin. Tissue sections were stained with hematoxylin and eosin. Lauren's classification for gastric cancer was used, to analyse the prevalence of H. pylori in intestinal or diffuse carcinomas assessed by the urease rapid test, IgG by ELISA and Giemsa staining. H. pylori infected patients were treated with omeprazole, clarithromycin and amoxicillin for 7 days. Follow-up endoscopy and serology were performed 6 months after treatment to determine successful eradication of H. pylori in non-tumor tissue. Thereafter, follow-up endoscopies were scheduled annually. Chi-square and MacNemar tests with 0.05 significance were used. RESULTS: Thirty-four tumors (60.7%) were intestinal-type and 22 (39.3%) diffuse type carcinomas. In adjacent non-tumor gastric mucosa, chronic gastritis were found in 53 cases (94.6%) and atrophic mucosa in 36 patients (64.3%). All the patients with atrophic mucosa were H. pylori positive. When examined by Giemsa and urease test, H. pylori positive rate in tumor tissue of intestinal type carcinomas was higher than that in diffuse carcinomas. In tumor tissues, 34 (60.7%) H. pylori-positive in gastric carcinomas were detected by Giemsa method. H. pylori was observed in 30 of 56 cases (53.5%) in tissues 4 cm adjacent to tumors. This difference was not significant. Eradication of H. pylori in non-tumor tissue of gastric remnant led to a complete negativity on the 12th postoperative month CONCLUSIONS: The data confirmed the hypothesis of a high prevalence of H. pylori in tumor tissue of gastric advanced carcinomas and in adjacent non-tumor mucosa of operated stomachs. The presence of H. pylori was predominant in the intestinal-type carcinoma.


Asunto(s)
Carcinoma/microbiología , Infecciones por Helicobacter/diagnóstico , Helicobacter pylori/aislamiento & purificación , Neoplasias Gástricas/microbiología , Estómago/microbiología , Adulto , Anciano , Colorantes Azulados , Biopsia , Carcinoma/patología , Ensayo de Inmunoadsorción Enzimática , Femenino , Infecciones por Helicobacter/complicaciones , Humanos , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Estómago/patología , Neoplasias Gástricas/patología , Ureasa/análisis
5.
Acta Cir Bras ; 21 Suppl 4: 18-22, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-17293960

RESUMEN

PURPOSE: To investigate the role of beta-(1-3)-D-glucan on 99mTc labelled Escherichia coli translocation and cytokines secretion in rats submitted to small bowel ischemia/reperfusion injury. METHODS: Five groups (n=10 each) of Wistar rats were subjected to control(C), sham(S), group IR subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R), and group I/R+glucan subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R) and injected with 2 mg/Kg intramuscular. Translocation of labelled bacteria to mesenteric lymph nodes, liver, spleen, lung and serum was determined using radioactivity/count and colony forming units/g(CFU/g). Serum TNFalpha, IL-1beta, IL-6, IL-10 were measured by ELISA. RESULTS: CFU/g and radioactivity/count were higher in I/R than in I/R+glucan rats. In C, S and S+glucan groups, bacteria and radioactivity/count were rarely detected. The I/R+glucan rats had enhancement of IL-10 and suppressed production of serum TNFalpha, IL-1beta and, IL-6, compared to I/R untreated animals. CONCLUSION: The beta-(1-3)-D-glucan modulated the production of pro-inflammatory and anti-inflammatory cytokines during bowel ischemia/reperfusion, and attenuated translocation of labelled bacteria.


Asunto(s)
Traslocación Bacteriana/efectos de los fármacos , Escherichia coli/fisiología , Intestino Delgado/irrigación sanguínea , Daño por Reperfusión/prevención & control , beta-Glucanos/farmacología , Animales , Traslocación Bacteriana/fisiología , Recuento de Colonia Microbiana , Citocinas/biosíntesis , Citocinas/metabolismo , Modelos Animales de Enfermedad , Mediadores de Inflamación/fisiología , Mucosa Intestinal/microbiología , Mucosa Intestinal/fisiología , Masculino , Proteoglicanos , Ratas , Ratas Wistar , Daño por Reperfusión/microbiología , Factor de Necrosis Tumoral alfa/sangre
6.
Braz J Infect Dis ; 9(4): 310-4, 2005 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-16270123

RESUMEN

We examined prevention of surgical site infection (SSI) in a tertiary teaching hospital in northeast Brazil, from January 1994 to December 2003. The survey included 5,742 patients subjected to thoracic, urologic, vascular and general surgery. The criteria for diagnosing SSI were those of the Centers for Disease Control, USA, and the variables of the National Nosocomial Infection Surveillance risk index were used. Data analysis revealed that anesthetic risk scores, wound class and duration of surgery were significantly associated with SSI. A total of 296 SSIs were detected among the 5,742 patients (5.1%). The overall incidence of SSI was 8.8% in 1994; it decreased to 3.3% in 2003. In conclusion, the use of educational strategies, based on guidelines for SSI prevention reduced SSI incidence. Appropriate management of preoperative, intraoperative, and postoperative incision care, and a surveillance system based on international criteria, were useful in reducing SSI rates in our hospital.


Asunto(s)
Infección Hospitalaria/epidemiología , Control de Infecciones/métodos , Vigilancia de la Población , Infección de la Herida Quirúrgica/epidemiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Brasil/epidemiología , Infección Hospitalaria/prevención & control , Femenino , Hospitales Universitarios , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Factores de Riesgo , Infección de la Herida Quirúrgica/prevención & control
7.
Braz J Otorhinolaryngol ; 81(4): 374-83, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26120097

RESUMEN

INTRODUCTION: The clinical evaluation of subjects with occupational noise exposure has been difficult due to the discrepancy between auditory complaints and auditory test results. This study aimed to evaluate the contralateral acoustic reflex thresholds of workers exposed to high levels of noise, and to compare these results to the subjects' auditory complaints. METHODS: This clinical retrospective study evaluated 364 workers between 1998 and 2005; their contralateral acoustic reflexes were compared to auditory complaints, age, and noise exposure time by chi-squared, Fisher's, and Spearman's tests. RESULTS: The workers' age ranged from 18 to 50 years (mean=39.6), and noise exposure time from one to 38 years (mean=17.3). We found that 15.1% (55) of the workers had bilateral hearing loss, 38.5% (140) had bilateral tinnitus, 52.8% (192) had abnormal sensitivity to loud sounds, and 47.2% (172) had speech recognition impairment. The variables hearing loss, speech recognition impairment, tinnitus, age group, and noise exposure time did not show relationship with acoustic reflex thresholds; however, all complaints demonstrated a statistically significant relationship with Metz recruitment at 3000 and 4000Hz bilaterally. CONCLUSION: There was no significance relationship between auditory complaints and acoustic reflexes.


Asunto(s)
Umbral Auditivo/fisiología , Pérdida Auditiva Provocada por Ruido/diagnóstico , Ruido en el Ambiente de Trabajo/efectos adversos , Exposición Profesional/efectos adversos , Reflejo Acústico/fisiología , Adulto , Femenino , Pérdida Auditiva Provocada por Ruido/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de Tiempo , Adulto Joven
8.
Biomed Res Int ; 2015: 201494, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25705651

RESUMEN

INTRODUCTION: Noise is a major cause of health disorders in workers and has unique importance in the auditory analysis of people exposed to it. The purpose of this study is to evaluate the arithmetic mean of the auditory thresholds at frequencies of 3, 4, and 6 kHz of workers from five professional categories exposed to occupational noise. METHODS: We propose a retrospective cross-sectional cohort study to analyze 2.140 audiograms from seven companies having five sectors of activity: one footwear company, one beverage company, two ceramics companies, two metallurgical companies, and two transport companies. RESULTS: When we compared two categories, we noticed a significant difference only for cargo carriers in comparison to the remaining categories. In all activity sectors, the left ear presented the worst values, except for the footwear professionals (P > 0.05). We observed an association between the noise exposure time and the reduction of audiometric values for both ears. Significant differences existed for cargo carriers in relation to other groups. This evidence may be attributed to different forms of exposure. A slow and progressive deterioration appeared as the exposure time increased.


Asunto(s)
Umbral Auditivo/fisiología , Ruido en el Ambiente de Trabajo/efectos adversos , Enfermedades Profesionales/fisiopatología , Adulto , Audiometría/métodos , Estudios Transversales , Humanos , Exposición Profesional/efectos adversos , Estudios Retrospectivos
9.
Braz J Otorhinolaryngol ; 80(3): 191-5, 2014.
Artículo en Inglés, Portugués | MEDLINE | ID: mdl-25153101

RESUMEN

INTRODUCTION: Obstructive sleep apnea syndrome has a high prevalence among adults. Cephalometric variables can be a valuable method for evaluating patients with this syndrome. OBJECTIVE: To correlate cephalometric data with the apnea-hypopnea sleep index. METHODS: We performed a retrospective and cross-sectional study that analyzed the cephalometric data of patients followed in the Sleep Disorders Outpatient Clinic of the Discipline of Otorhinolaryngology of a university hospital, from June 2007 to May 2012. RESULTS: Ninety-six patients were included, 45 men, and 51 women, with a mean age of 50.3 years. A total of 11 patients had snoring, 20 had mild apnea, 26 had moderate apnea, and 39 had severe apnea. The distance from the hyoid bone to the mandibular plane was the only variable that showed a statistically significant correlation with the apnea-hypopnea index. CONCLUSION: Cephalometric variables are useful tools for the understanding of obstructive sleep apnea syndrome. The distance from the hyoid bone to the mandibular plane showed a statistically significant correlation with the apnea-hypopnea index.


Asunto(s)
Cefalometría/métodos , Apnea Obstructiva del Sueño/fisiopatología , Ronquido/fisiopatología , Adolescente , Adulto , Anciano , Estudios Transversales , Femenino , Humanos , Masculino , Persona de Mediana Edad , Polisomnografía , Estudios Retrospectivos , Índice de Severidad de la Enfermedad , Adulto Joven
10.
Braz. j. otorhinolaryngol. (Impr.) ; 81(4): 374-383, July-Aug. 2015. tab
Artículo en Inglés | LILACS | ID: lil-758006

RESUMEN

INTRODUCTION: The clinical evaluation of subjects with occupational noise exposure has been difficult due to the discrepancy between auditory complaints and auditory test results. This study aimed to evaluate the contralateral acoustic reflex thresholds of workers exposed to high levels of noise, and to compare these results to the subjects' auditory complaints.METHODS: This clinical retrospective study evaluated 364 workers between 1998 and 2005; their contralateral acoustic reflexes were compared to auditory complaints, age, and noise exposure time by chi-squared, Fisher's, and Spearman's tests.RESULTS: The workers' age ranged from 18 to 50 years (mean = 39.6), and noise exposure time from one to 38 years (mean = 17.3). We found that 15.1% (55) of the workers had bilateral hearing loss, 38.5% (140) had bilateral tinnitus, 52.8% (192) had abnormal sensitivity to loud sounds, and 47.2% (172) had speech recognition impairment. The variables hearing loss, speech recognition impairment, tinnitus, age group, and noise exposure time did not show relationship with acoustic reflex thresholds; however, all complaints demonstrated a statistically significant relationship with Metz recruitment at 3000 and 4000 Hz bilaterally.CONCLUSION: There was no significance relationship between auditory complaints and acoustic reflexes.


INTRODUÇÃO: A avaliação clínico-ocupacional de trabalhadores expostos a ruído é dificultada pela discrepância entre queixas auditivas e resultados dos exames audiológicos. Este estudo pretende avaliar limiares dos reflexos estapedianos contralaterais em sujeitos expostos a níveis elevados de pressão sonora, relacionando-os com queixas auditivas.MÉTODO: Estudo clínico retrospectivo que analisou 364 trabalhadores e seus limiares de reflexos estapedianos contralaterais, relacionado-os com queixas auditivas, idades e tempos de exposição ao ruído.RESULTADOS: Dos trabalhadores avaliados, com idades de 18 a 50 anos (média 39,6) e tempos de exposição entre um e 38 anos (média 17,3); 15,1% (55) tinham queixa de perda auditiva bilateral, 38,5% (140) zumbidos bilaterais, 52,8% (192) irritação ao ouvir sons intensos e 47,2% (172) dificuldades para reconhecer a fala. As variáveis: perda auditiva, dificuldade para reconhecimento da fala, zumbidos, faixa etária e tempo de exposição ao ruído não se relacionaram significativamente com limiares dos reflexos estapedianos, mas todas as queixas apresentaram relação estatisticamente significante com o recrutamento de Metz nas frequências de 3000 e 4000 Hz, bilateralmente.CONCLUSÃO: Não houve relações significativas entre limiares dos reflexos estapedianos e queixas auditivas.


Asunto(s)
Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Umbral Auditivo/fisiología , Pérdida Auditiva Provocada por Ruido/diagnóstico , Ruido en el Ambiente de Trabajo/efectos adversos , Exposición Profesional/efectos adversos , Reflejo Acústico/fisiología , Pérdida Auditiva Provocada por Ruido/fisiopatología , Estudios Retrospectivos , Factores de Tiempo
11.
Braz. j. otorhinolaryngol. (Impr.) ; 80(3): 191-195, May-June/2014. tab, graf
Artículo en Inglés | LILACS | ID: lil-712983

RESUMEN

INTRODUCTION: Obstructive sleep apnea syndrome has a high prevalence among adults. Cephalometric variables can be a valuable method for evaluating patients with this syndrome. OBJECTIVE: To correlate cephalometric data with the apnea-hypopnea sleep index. METHODS: We performed a retrospective and cross-sectional study that analyzed the cephalometric data of patients followed in the Sleep Disorders Outpatient Clinic of the Discipline of Otorhinolaryngology of a university hospital, from June 2007 to May 2012. RESULTS: Ninety-six patients were included, 45 men, and 51 women, with a mean age of 50.3 years. A total of 11 patients had snoring, 20 had mild apnea, 26 had moderate apnea, and 39 had severe apnea. The distance from the hyoid bone to the mandibular plane was the only variable that showed a statistically significant correlation with the apnea-hypopnea index. CONCLUSION: Cephalometric variables are useful tools for the understanding of obstructive sleep apnea syndrome. The distance from the hyoid bone to the mandibular plane showed a statistically significant correlation with the apnea-hypopnea index. .


INTRODUÇÃO: A síndrome da apneia do sono apresenta grande prevalência na população adulta. A cefalometria com análise das variáveis morfológicas pode ser um método valioso na avaliação de pacientes com essa síndrome. OBJETIVO: Correlacionar dados cefalométricos com o índice de apneia-hipopneia do sono, com a finalidade de detectar fatores preditores para a gravidade da síndrome da apneia obstrutiva do sono. MÉTODOS: Trata-se de um estudo retrospectivo, em que foram analisadas cefalometrias de pacientes em acompanhamento no ambulatório de distúrbios do sono da disciplina de Otorrinolaringologia, de um hospital universitário terciário, no período de junho de 2007 a maio de 2012. RESULTADOS: Foram avaliados 96 pacientes, sendo 45 homens e 51 mulheres. Onze pacientes eram portadores de roncopatia, 20 de apneia leve, 26 de apneia moderada e 39 de apneia grave. A única variável cefalométrica que apresentou correlação estatisticamente significante com o índice de apneia e hipoapneia foi a distância linear perpendicular do osso hioide ao plano mandibular. CONCLUSÃO: As variáveis cefalométricas podem ser úteis no entendimento da síndrome da apneia obstrutiva do sono e uma atenção deve ser dada à variável que mede a distância do hioide perpendicularmente ao plano mandibular. .


Asunto(s)
Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Cefalometría/métodos , Apnea Obstructiva del Sueño/fisiopatología , Ronquido/fisiopatología , Estudios Transversales , Polisomnografía , Estudios Retrospectivos , Índice de Severidad de la Enfermedad
12.
Arq. gastroenterol ; 43(4): 288-292, out.-dez. 2006. tab
Artículo en Inglés | LILACS | ID: lil-445632

RESUMEN

BACKGROUD: There is substantial evidence that infection with Helicobacter pylori plays a role in the development of gastric cancer and that it is rarely found in gastric biopsy of atrophic gastritis and gastric cancer. On advanced gastric tumors, the bacteria can be lost from the stomach. AIMS: To analyze the hypothesis that the prevalence of H.pylori in operated advanced gastric carcinomas and adjacent non-tumor tissues is high, comparing intestinal and diffuse tumors according to Lauren's classification METHODS: A prospective controlled study enrolled 56 patients from "Hospital Universitário", Federal University of Rio Grande do Norte, Natal, RN, Brazil, with advanced gastric cancer, treated from February 2000 to March 2003. Immediately after partial gastrectomy, the resected stomach was opened and several mucosal biopsy samples were taken from the gastric tumor and from the adjacent mucosa within 4 cm distance from the tumor margin. Tissue sections were stained with hematoxylin and eosin. Lauren's classification for gastric cancer was used, to analyse the prevalence of H. pylori in intestinal or diffuse carcinomas assessed by the urease rapid test, IgG by ELISA and Giemsa staining. H. pylori infected patients were treated with omeprazole, clarithromycin and amoxicillin for 7 days. Follow-up endoscopy and serology were performed 6 months after treatment to determine successful eradication of H. pylori in non-tumor tissue. Thereafter, follow-up endoscopies were scheduled annually. Chi-square and MacNemar tests with 0.05 significance were used. RESULTS: Thirty-four tumors (60.7 percent) were intestinal-type and 22 (39.3 percent) diffuse type carcinomas. In adjacent non-tumor gastric mucosa, chronic gastritis were found in 53 cases (94.6 percent) and atrophic mucosa in 36 patients (64.3 percent). All the patients with atrophic mucosa were H. pylori positive. When examined by Giemsa and urease test, H. pylori positive rate in tumor...


RACIONAL: Existe evidência de que a infecção pelo Helicobacter pylori desempenha papel importante na causa do câncer gástrico e que é raramente encontrada em biopsias de gastrite atrófica e em tecido tumoral de câncer do estômago. Com a evolução para câncer gástrico avançado, a bactéria tende a desaparecer do tecido tumoral OBJETIVOS: Analisar a prevalência do H. pylori em peças operatórias de carcinomas gástricos avançados e no tecido adjacente aos tumores, comparando os tumores tipo intestinal e difuso de acordo com a classificação de Lauren MÉTODOS: Estudo prospectivo controlado incluiu 56 pacientes operados no Hospital Universitário da Universidade Federal do Rio Grande do Norte, Natal, RN, com câncer gástrico avançado, entre fevereiro de 2000 e março de 2003. Imediatamente após a gastrectomia, a peça operatória foi aberta e foram feitas várias biopsias do tecido neoplásico e da mucosa adjacente a 4 cm da margem tumoral. Os tecidos formam processados e corados pela hematoxilina-eosina. Foi usada a classificação de Lauren para carcinoma gástrico. A infecção pelo H. pylori foi diagnosticada pelo teste da urease, dosagem de IgG por ELISA e histopatologia com coloração Giemsa. Os pacientes infectados pelo H. pylori foram tratados com omeprazol, claritromicina e amoxicilina por 7 dias. Após 6 meses, 1 ano e 2 anos, foi feito seguimento utilizando endoscopia, dosagem de IgG e teste da urease para avaliar o sucesso da erradicação do H. pylori e recidiva do tumor RESULTADOS: O carcinoma tipo intestinal ocorreu em 34 (60,7 por cento) pacientes e 22 (39,3 por cento) foram acometidos de carcinoma difuso. No tecido adjacente não-tumoral a gastrite crônica foi observada em 53 casos (94,6 por cento) e mucosa atrófica em 36 pacientes (64,3 por cento), todos H. pylori positivos. Exames pelo Giemsa e teste da urease revelaram maior prevalência de H. pylori positivo no tecido tumoral do carcinoma tipo intestinal do que no tipo difuso. Quando foi comparada...


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Carcinoma/microbiología , Infecciones por Helicobacter/diagnóstico , Helicobacter pylori/aislamiento & purificación , Neoplasias Gástricas/microbiología , Estómago/microbiología , Colorantes Azulados , Biopsia , Carcinoma/patología , Ensayo de Inmunoadsorción Enzimática , Infecciones por Helicobacter/complicaciones , Estudios Prospectivos , Neoplasias Gástricas/patología , Estómago/patología , Ureasa/análisis
13.
Acta cir. bras ; 21(supl.4): 18-22, 2006.
Artículo en Inglés | LILACS | ID: lil-440773

RESUMEN

PURPOSE: To investigate the role of beta-(1-3)-D-glucan on 99mTc labelled Escherichia coli translocation and cytokines secretion in rats submitted to small bowel ischemia/reperfusion injury. METHODS: Five groups (n=10 each) of Wistar rats were subjected to control(C), sham(S), group IR subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R), and group I/R+glucan subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R) and injected with 2mg/Kg intramuscular. Translocation of labelled bacteria to mesenteric lymph nodes, liver, spleen, lung and serum was determined using radioactivity/count and colony forming units/g(CFU/g). Serum TNFalpha, IL-1beta, IL-6, IL-10 were measured by ELISA. RESULTS: CFU/g and radioactivity/count were higher in I/R than in I/R+glucan rats. In C, S and S+glucan groups, bacteria and radioactivity/count were rarely detected. The I/R+glucan rats had enhancement of IL-10 and suppressed production of serum TNFalpha, IL-1beta and, IL-6, compared to I/R untreated animals. CONCLUSION: The beta-(1-3)-D-glucan modulated the production of pro-inflammatory and anti-inflammatory cytokines during bowel ischemia/reperfusion, and attenuated translocation of labelled bacteria.


OBJETIVO: Investigar o papel da beta-(1-3)-D-glucana na translocação de Escherichia coli marcada com 99mTc e na secreção de citocinas em ratos submetidos a isquemia e reperfusão intestinal. MÉTODOS: Cinco grupos (n=10 cada) de ratos Wistar foram denominados controle (C), sham (S), grupo IR submetido a 45 minutos de isquemia do intestino delgado e 60 minutos de reperfusão(I/R), grupo I/R+glucana com 45 minutos de isquemia e 60 minutos de reperfusão(I/R) e tratados com glucana 2mg/Kg intramuscular. Translocação de Escherichia coli marcada com 99mTc, para Linfonodos mesentéricos, fígado, baço, pulmão e soro foi avaliada usando contagem de radioatividade e de unidades formadoras de colônias/g (UFC/g) Dosagem sérica de TNFalfa, IL-1beta, IL-6, IL-10 foi realizada pelo método ELISA. RESULTADOS: CFU/g e contagem de radioatividade foi significantemente maior nos ratos do grupo I/R do que no grupo I/R+glucana. Nos grupos C, S e S+glucana bactérias e contagem radioativa foram raramente detectadas. Os ratos do grupo I/R+glucana tiveram aumento de IL-10 sérica e significante redução da expressão de TNFalfa, IL-1beta e IL-6, quando comparados com os animais não tratados do grupo I/R. CONCLUSÃO: A beta-(1-3)-D-glucana modulou a produção de citocinas pró-inflamatórias e anti-inflamatórias durante a isquemia/reperfusão intestinal e contribuiu para reduzir a translocação de bactérias marcadas.


Asunto(s)
Animales , Masculino , Ratas , Traslocación Bacteriana/efectos de los fármacos , Escherichia coli/fisiología , Intestino Delgado/irrigación sanguínea , Daño por Reperfusión/prevención & control , beta-Glucanos/farmacología , Traslocación Bacteriana/fisiología , Recuento de Colonia Microbiana , Citocinas/biosíntesis , Citocinas , Modelos Animales de Enfermedad , Mediadores de Inflamación/fisiología , Mucosa Intestinal/microbiología , Mucosa Intestinal/fisiología , Ratas Wistar , Daño por Reperfusión/microbiología , Factor de Necrosis Tumoral alfa/sangre
14.
Braz. j. infect. dis ; 9(4): 310-314, Aug. 2005. tab, graf
Artículo en Inglés | LILACS | ID: lil-415685

RESUMEN

We examined prevention of surgical site infection (SSI) in a tertiary teaching hospital in northeast Brazil, from January 1994 to December 2003. The survey included 5,742 patients subjected to thoracic, urologic, vascular and general surgery. The criteria for diagnosing SSI were those of the Centers for Disease Control, USA, and the variables of the National Nosocomial Infection Surveillance risk index were used. Data analysis revealed that anesthetic risk scores, wound class and duration of surgery were significantly associated with SSI. A total of 296 SSIs were detected among the 5,742 patients (5.1 percent). The overall incidence of SSI was 8.8 percent in 1994; it decreased to 3.3 percent in 2003. In conclusion, the use of educational strategies, based on guidelines for SSI prevention reduced SSI incidence. Appropriate management of preoperative, intraoperative, and postoperative incision care, and a surveillance system based on international criteria, were useful in reducing SSI rates in our hospital.


Asunto(s)
Humanos , Masculino , Femenino , Adolescente , Adulto , Persona de Mediana Edad , Infección Hospitalaria/epidemiología , Hospitales Universitarios , Control de Infecciones/métodos , Vigilancia de la Población , Infección de la Herida Quirúrgica/epidemiología , Brasil , Infección Hospitalaria/prevención & control , Incidencia , Factores de Riesgo , Infección de la Herida Quirúrgica/prevención & control
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