RESUMEN
Two hundred fifteen compounds isolated from plants of Northeastern Brazil flora have been assayed against epimastigote forms of Trypanosoma cruzi, using the tetrazolium salt MTT as an alternative method. Eight compounds belonging to four different species: Harpalyce brasiliana (Fabaceae), Acnistus arborescens and Physalis angulata (Solanaceae), and Cordia globosa (Boraginaceae) showed significant activity. Among them, a novel and a known pterocarpan, a chalcone, four withasteroids, and a meroterpene benzoquinone were the represented chemical classes.
Asunto(s)
Plantas Medicinales/química , Pterocarpanos/aislamiento & purificación , Pterocarpanos/farmacología , Tripanocidas/aislamiento & purificación , Trypanosoma cruzi/efectos de los fármacos , Animales , Células Sanguíneas/parasitología , Brasil , Células Cultivadas , Chalcona , Humanos , Concentración 50 Inhibidora , Espectroscopía de Resonancia Magnética , Estructura Molecular , Pterocarpanos/química , Relación Estructura-Actividad , Tripanocidas/química , Tripanocidas/farmacologíaRESUMEN
A NMR study of two new pterocarpans isolated from the roots of Harpalyce brasiliana is described. In addition to 1D NMR, 2D shift-correlated NMR pulse sequences ((1)H-(1)H-COSY, HSQC and HMBC) were used to establish the structures, and unambiguously perform the (1)H and (13)C chemical shift assignments.