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1.
Int J Environ Health Res ; 33(10): 976-992, 2023 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-35466819

RESUMEN

To review the applicability and accessibility of physical activity guidelines for adults living with long-term conditions whilst shielding during the COVID-19. A narrative review with systematic methodology was conducted between 2015 and 2021, with two stages: 1) Search of electronic databases PubMed/Medline, Web of Science, PsycInfo, and Cinahl; 2) search of long-term condition organisations. Sixty-five articles were identified, where nine included specific guidelines during the COVID-19, 28 specific guidelines to individuals living with long-term conditions and 7 identified the utilization of online resources. Twenty-one long-term condition organizations websites were reviewed where all of them included a section regarding physical activity guidelines and seven referred to online and offline accessible resources during COVID-19. Accessibility and applicability were variable across academic databases and long-term conditions organisation websites. Findings could inform long-term condition policy and guidelines development to better and more relevant support people living with long-term conditions to be physically active.


Asunto(s)
COVID-19 , Adulto , Humanos , COVID-19/prevención & control , Ejercicio Físico
2.
BMC Fam Pract ; 19(1): 45, 2018 04 05.
Artículo en Inglés | MEDLINE | ID: mdl-29621985

RESUMEN

BACKGROUND: Parkinson's disease progressively limits patients at different levels and as a result family members play a key role in their care. However, studies show lack of an integrative approach in Primary Care to respond to the difficulties and psychosocial changes experienced by them. The aim of this study is to evaluate the effects of a multidisciplinary psychoeducational intervention focusing on improving coping skills, the psychosocial adjustment to Parkinson's disease and the quality of life in patients and family carers in a Primary Care setting. METHODS: This quasi-experimental study with control group and mixed methods was designed to evaluate a multidisciplinary psychoeducational intervention. Based on the study power calculations, 100 people with Parkinson's disease and 100 family carers will be recruited and assigned to two groups. The intervention group will receive the ReNACE psychoeducational intervention. The control group will be given a general educational programme. The study will be carried out in six community-based health centres. The results obtained from the two groups will be collected for evaluation at three time points: at baseline, immediately after the intervention and at 6 months post-intervention. The results will be measured with these instruments: the Quality of Life Scale PDQ-39 for patients and the Scale of Quality of Life of Care-givers SQLC for family carers, and for all participants the Psychosocial Adjustment to Illness scale and the Brief COPE Inventory. Focus groups will be organised with some patients and family carers who will have received the ReNACE psychoeducational intervention and also with the healthcare professionals involved in its development. DISCUSSION: An important gap exists in the knowledge and application of interventions with a psychosocial approach for people with PD and family carers as a whole. This study will promote this comprehensive approach in Primary Care, which will clearly contribute in the existing knowledge and could reduce the burden of PD for patients and family carers, and also in other long-term conditions. TRIAL REGISTRATION: NCT03129425 (ClinicalTrials.gov). Retrospectively registered on April 26, 2017.


Asunto(s)
Cuidadores/educación , Educación en Salud/métodos , Enfermedad de Parkinson/terapia , Calidad de Vida , Adulto , Humanos , Educación del Paciente como Asunto/métodos , Proyectos de Investigación , España
3.
Radiologia ; 59(6): 487-495, 2017.
Artículo en Inglés, Español | MEDLINE | ID: mdl-28923493

RESUMEN

There are different magnetic resonance techniques and models to quantify liver iron concentration. T2 relaxometry methods evaluate the iron concentration in the myocardium, and they are able to discriminate all the levels of iron overload in the liver. Signal intensity ratio methods saturate with high levels of liver overload and can not assess iron concentration in the myocardium but they are more accessible and are very standardized. This article reviews, in different clinical scenarios, when Magnetic Resonance must be used to assess iron overload in the liver and myocardium and analyzes the current challenges to optimize the aplication of the technique and to be it included in the clinical guidelines.


Asunto(s)
Sobrecarga de Hierro/diagnóstico , Espectroscopía de Resonancia Magnética , Humanos , Hígado/química , Miocardio/química , Reproducibilidad de los Resultados
4.
Microb Ecol ; 68(4): 729-39, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-24889287

RESUMEN

Understanding the distribution of bacteria is a major goal of microbial ecology which remains to be fully deciphered. In this study, a model 50 °C temperature gradient at a Northern Thailand hot spring was analyzed to determine how the bacterial communities were structured in the environment. Communities were examined through 16S rRNA gene amplification, denaturing gradient gel electrophoresis, and sequencing. The two major phyla, Cyanobacteria and Chloroflexi, showed characteristic distributions along the temperature gradient. Different clades were allocated at specific portions of the gradient. Comparisons of the bacterial communities along the temperature gradient showed sharp decreases of similarity at increasing temperature difference. Peaks of maximum richness were observed at 50 and 70 °C. This study contributes to explain how environmental conditions and microbial interactions can influence the distribution of specific bacterial clades and phyla shaping the structure of microbial communities in nature.


Asunto(s)
Biodiversidad , Chloroflexi/fisiología , Cianobacterias/fisiología , Manantiales de Aguas Termales/microbiología , Chloroflexi/genética , Cianobacterias/genética , ADN Bacteriano/genética , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Tailandia
5.
Enferm Intensiva ; 24(2): 72-88, 2013.
Artículo en Español | MEDLINE | ID: mdl-23375829

RESUMEN

UNLABELLED: The optimum transition process from the ICU to the ward is key to avoiding the appearance of anxiety in the patient and family, increase of re-admissions in the ICU with the consequent increase in costs and jeopardization to the patient's safety. OBJECTIVE: 1) To identify, study and give a critical presentation of the existing evidence on how patients, families and nurses experience the transition from ICU to the ward; 2) to analyze the possible interventions available for the development of an optimum transition process. METHODOLOGY: A review was made of the evidence available in the main databases. In addition, several journals specialized in Intensive Care were reviewed. Studies with a qualitative, quantitative or mixed approach and reviews on the subject with a systematic methodology or narrative reviews were included. RESULTS: A total of 23 papers were selected for review, 10 of which were qualitative studies, 11 quantitative and two had combined methodology. «Transfer anxiety¼ was identified after the analysis of these articles as one of the recurring aspects. Discrepancies regarding who should take responsibility for the preparation of the transition process and when it should be performed were also found. In the literature reviewed, several interventions have been proposed to facilitate an optimal transition process such as developing information brochures, creating a profile of practicing nursing liaison between the ICU and the ward and ICU discharge report. CONCLUSIONS: This review emphasizes the importance of taking into account the perspectives of patients, families and nurses to perform optimal planning of the transition of the patient from the ICU to the ward to ensure their safety.


Asunto(s)
Unidades de Cuidados Intensivos , Transferencia de Pacientes , Habitaciones de Pacientes , Humanos , Satisfacción del Paciente
6.
Radiologia ; 55(4): 331-9, 2013.
Artículo en Español | MEDLINE | ID: mdl-22244013

RESUMEN

OBJECTIVE: To determine whether there is iron overload by calculating the T2* value in the liver and myocardium in patients with secondary haemochromatosis. To analyse the correlation of the values obtained with the iron levels in blood, with the liver iron concentration (LIC) calculated using magnetic resonance (MR) imaging, and the correlation between them. MATERIAL AND METHODS: A total of 16 patients (13 males, 3 females), with a mean age of 61 years, were included and evaluated in the years 2008 and 2009. Fifteen of them had received multiple transfusions, and one was diagnosed with hereditary sideroblastic anaemia. The measurements included, blood ferritin, LIC by MRI, cardiac function using MRI and the T2* value by means of multi-echo sequences in the liver (TR/TE1/ΔTE/No of echos/α: 21/1,18/1.0/20/35°) and myocardium (26/1.04/0.8/30/60°). A correlation-regression analysis was performed by comparing the cardiac and liver T2* values with the ferritin, LIC and between each of them. RESULTS: A total of 13 patients had ferritin values greater than 1000ng/ml (median/minimum/maximum: 1762/294/3785ng/ml). An increased LIC greater than 80µmol/g (median/minimum/maximum: 125.4/41.2/241.5µmol/g) was observed in 13 patients. In all cases cardiac function was conserved, and in 15 cases the liver T2* value was less than 6.3ms. The myocardium T2* value was less than 20ms. in only one case. A high correlation was observed between the liver T2* values and the LIC (r:-0.912). The correlation was statistically significant between the liver T2* value and ferritin (r:-0.541). The correlations between myocardium T2* and ferritin, myocardium T2* and LIC, and myocardium T2* and liver T2* were not statistically significant. CONCLUSIONS: The liver T2* showed a high correlation with LIC and a statistically significant correlation with ferritin. No association was observed between the myocardium T2* values and ferritin in blood, the LIC or the liver T2* value.


Asunto(s)
Hemocromatosis , Hierro/análisis , Hígado/química , Imagen por Resonancia Magnética , Miocardio/química , Adulto , Anciano , Femenino , Ferritinas/sangre , Hemocromatosis/sangre , Hemocromatosis/metabolismo , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven
7.
Res Involv Engagem ; 9(1): 37, 2023 May 31.
Artículo en Inglés | MEDLINE | ID: mdl-37259130

RESUMEN

BACKGROUND: Patient and public involvement (PPI) is increasingly seen as essential to health service research. There are strong moral and ethical arguments for good quality PPI. Despite the development of guidance aimed at addressing the inconsistent reporting of PPI activities within research, little progress has been made in documenting the steps taken to undertake PPI and how it influences the direction of a study. Without this information, there are minimal opportunities to share learnings across projects and strengthen future PPI practices. The aim of this paper is to present details on the processes and activities planned to integrate PPI into the qualitative research component of a mixed-methods, multi-site study evaluating the implementation of a smart template to promote personalised primary care for patients with multiple long-term conditions. METHODS: This proposal describes the processes and activities planned to integrate PPI into the development and piloting of qualitative data collection tools (topic guides for both practice staff and patients) and a tailored data analysis package developed for PPI members incorporating broad concepts and specific methods of qualitative data analysis. DISCUSSION: Outputs relating to PPI activity may include clear, concise and suitably worded topic guides for qualitative interviews. Piloting of the topic guides via mock interviews will further develop researchers' skills including sensitisation to the experiences of participants being interviewed. Working with PPI members when analysing the qualitative data aims to provide reciprocal learning opportunities and may contribute to improving the overall rigour of the data analysis. The intent of publishing proposed PPI activities within this project is to inform the future delivery of high quality PPI.


Patient and public involvement (PPI) improves healthcare research, however, there is little published evidence of proposed PPI activities within a research study. The aim of this article is to describe the proposed PPI activities which are to be integrated into a study implementing a smart template to promote personalised care for people with multiple long-term conditions within primary care in the United Kingdom. The proposal describes the ways in which PPI members will be included within the data collection and analysis phases of a research study which includes interviews with primary care staff and patients. PPI members will be asked to develop questions for these interviews and to take part in a mock interview whereby a researcher interviews a PPI member. The proposal also provides details on qualitative data analysis workshops which will be specifically developed for PPI members. The impact of PPI activities could include clear, concise and suitably worded questions used within the interviews. Piloting of these questions via mock interviews may enable researchers to further develop their interviewing skills. It is anticipated that involving PPI members when analysing qualitative data will provide opportunities for reciprocal learning and lead to rich interpretations of the data, inclusive of the PPI members' perspectives. Publishing a record of planned PPI activities and potential impacts demonstrates the rationale and considerations made by the team to ensure that involvement in this study is meaningful and has potential benefits for all involved. The team hopes this proposal will support others with the planning and delivery of PPI activities. In future publications, we will reflect on the learnings, challenges, and outcomes from the PPI activities detailed in this proposal.

8.
Naturwissenschaften ; 99(1): 43-53, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22159635

RESUMEN

Organic sulfur and nitrogen are major reservoirs of these elements in terrestrial systems, although their cycling remains to be fully understood. Both sulfur and nitrogen mineralization are directly related to microbial metabolism. Mesophiles and thermophiles were isolated from temperate environments. Thermophilic isolates were classified within the Firmicutes, belonging to the Geobacillus, Brevibacillus, and Ureibacillus genera, and showed optimum growth temperatures between 50°C and 60°C. Sulfate and ammonium produced were higher during growth of thermophiles both for isolated strains and natural bacterial assemblages. They were positively related to organic nutrient load. Temperature also affected the release of sulfate and ammonium by thermophiles. Quantitative, real-time reverse-transcription polymerase chain reaction on environmental samples indicated that the examined thermophilic Firmicutes represented up to 3.4% of the total bacterial community RNA. Temperature measurements during summer days showed values above 40°C for more than 10 h a day in soils from southern Spain. These results support a potential role of thermophilic bacteria in temperate terrestrial environments by mineralizing organic sulfur and nitrogen ruled by the existence and length of warm periods.


Asunto(s)
Ecosistema , Bacterias Grampositivas/fisiología , Nitrógeno/metabolismo , Microbiología del Suelo , Azufre/metabolismo , Bacterias Grampositivas/aislamiento & purificación , Datos de Secuencia Molecular , Compuestos de Amonio Cuaternario/análisis , ARN Ribosómico 16S/genética , Sulfatos/análisis
9.
Appl Environ Microbiol ; 77(1): 351-4, 2011 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-21075891

RESUMEN

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.


Asunto(s)
Biodiversidad , Dermatoglifia del ADN/métodos , Electroforesis en Gel de Gradiente Desnaturalizante/métodos , Técnicas Microbiológicas/métodos , Reactores Biológicos/microbiología , Análisis por Conglomerados , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN
10.
Extremophiles ; 13(2): 321-31, 2009 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-19109691

RESUMEN

The microbial communities of freshwater hot spring mats from Boekleung (Western Thailand) were studied. Temperatures ranged from over 50 up to 57 degrees C. Green-, red-, and yellow colored mat layers were analyzed. In order to detect the major components of the microbial communities constituting the mat as well as the microorganisms showing significant metabolic activity, samples were analyzed using DNA- and RNA-based molecular techniques, respectively. Microbial community fingerprints, performed by denaturing gradient gel electrophoresis (DGGE), revealed clear differences among mat layers. Thermophilic phototrophic microorganisms, Cyanobacteria and Chloroflexi, constituted the major groups in these communities (on average 65 and 51% from DNA and RNA analyses, respectively). Other bacteria detected in the mat were Bacteroidetes, members of the Candidate Division OP10, Actinobacteria, and Planctomycetes. Differently colored mat layers showed characteristic bacterial communities and the major components of the metabolically active fraction of these communities have been identified.


Asunto(s)
Actinobacteria/fisiología , Bacteroidetes/fisiología , Chloroflexi/fisiología , Cianobacterias/fisiología , Bacterias/genética , Biodiversidad , Ecosistema , Electroforesis en Gel de Poliacrilamida/métodos , Agua Dulce/microbiología , Manantiales de Aguas Termales/microbiología , Filogenia , Análisis de Secuencia de ADN , Tailandia , Microbiología del Agua
11.
Naturwissenschaften ; 96(9): 1035-42, 2009 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19488732

RESUMEN

Bacteria thriving in underground systems, such as karsts, adapt to use a variety of nutrients. Most of these nutrients derive from superficial processes. This study shows that bacteria are able to differentially induce carbonate precipitation or dissolution depending on the availability of nutrients for growth. Different bacterial strains isolated from caves, representing the most common components of these microbial communities, were cultured with different carbon and nitrogen sources (e.g., acetate, glucose, peptone, humic acids) and induced changes in pH were measured during growth. Carbonate can either precipitate or dissolve during bacterial growth. The induction of carbonate precipitates or their dissolution as a function of consumption of specific carbon sources revealed the existence of an active nutrient cycling process in karsts and links nutrients and environmental conditions to the existence of a highly significant carbon sink in subterraneous environments.


Asunto(s)
Bacterias/crecimiento & desarrollo , Acetatos/metabolismo , Actinobacteria/crecimiento & desarrollo , Actinobacteria/metabolismo , Cloruro de Amonio/metabolismo , Bacillus/crecimiento & desarrollo , Bacillus/metabolismo , Bacterias/aislamiento & purificación , Bacterias/metabolismo , Reactores Biológicos , Carbono/metabolismo , Carbonatos/metabolismo , Medios de Cultivo , Glucosa/metabolismo , Sustancias Húmicas , Concentración de Iones de Hidrógeno , Nitratos/metabolismo , Peptonas/metabolismo , Proteobacteria/crecimiento & desarrollo , Proteobacteria/metabolismo , España , Staphylococcus/crecimiento & desarrollo , Staphylococcus/metabolismo
12.
Antonie Van Leeuwenhoek ; 96(4): 559-67, 2009 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19672687

RESUMEN

Differently colored layers of freshwater hot spring mats at Boekleung (Western Thailand) were studied. Temperatures ranged from over 50 up to 57 degrees C. Two mats were characterized: a laminated mat with a green and a red layers, and a monolayer, greenish-yellow mat. Bacterial communities in green, red, and yellow layers were investigated using molecular, culturing and pigment analysis methods. Pigment profiles covered a wide spectrum from chlorophylls to carotenoids. A green mat layer showed higher relative content of chlorophyll than yellow and red layers which presented higher proportion of carotenoids. Cyanobacterial isolates grow up to 55-56 degrees C and their pigment profiles showed a relatively high content of chlorophylls suggesting the importance of other bacterial groups in the mat pigment profiles. Bacterial communities were analyzed by 16S rDNA surveys showing Cyanobacteria and Chloroflexi as the mayor components of the community. Other significant members were Candidate Division OP10, Bacteroidetes, Planctomycetes and Actinobacteria. These results highlight a major participation of Cyanobacteria and Chloroflexi in thermal mat communities, and the preferential presence of Candidate Division OP10 in green mat layers. Differently colored mat layers showed characteristic bacterial communities which could be discriminated from pigment profiles and molecular surveys.


Asunto(s)
Bacterias/clasificación , Bacterias/metabolismo , Sedimentos Geológicos/microbiología , Manantiales de Aguas Termales , Pigmentos Biológicos/biosíntesis , Bacterias/genética , Bacterias/aislamiento & purificación , Carotenoides/análisis , Clorofila/análisis , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Calor , Pigmentos Biológicos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Tailandia
13.
J Appl Genet ; 50(4): 421-30, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-19875895

RESUMEN

The presence and distribution of CRISPR (clustered regularly interspaced short palindrome repeat) elements in the archaeal order Thermococcales were analyzed. Four complete genome sequences from the species Pyrococcus abyssi, P. furiosus, P. horikoshii, and Thermococcus kodakaraensis were studied. A fragment of the genome of P. furiosus was flanked by CRISPR elements upstream and by a single element downstream. The composition of the gene sequences contained in this genome fragment (positions 699013 to 855319) showed significant differences from the other genes in the P. furiosus genome. Differences were observed in the GC content at the third codon positions and the frequency of codon usage between the genes located in the analyzed fragment and the other genes in the P. furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea.


Asunto(s)
Transferencia de Gen Horizontal , Pyrococcus furiosus/genética , Thermococcales/genética , Composición de Base , Secuencia de Bases , Codón/genética , ADN de Archaea/química , ADN de Archaea/genética , Genoma Arqueal , Filogenia , Pyrococcus abyssi/genética , Pyrococcus horikoshii/genética , Homología de Secuencia de Ácido Nucleico , Elementos de Nucleótido Esparcido Corto , Especificidad de la Especie , Thermococcus/genética
14.
J Appl Microbiol ; 104(3): 681-91, 2008 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-17927740

RESUMEN

AIMS: To determine the major components of total and metabolically active microbial communities of yellow and grey colonizations threatening the conservation of palaeolithic paintings in Altamira Cave (Spain). METHODS AND RESULTS: Micro-organisms present in yellow and grey colonizations were determined from DNA analysis with those showing metabolic activity determined from RNA analysis. Microbial community fingerprints were obtained by denaturing gradient gel electrophoresis (DGGE) and 16S rDNA libraries were constructed from PCR amplified products. Proteobacteria was the most frequent bacterial phylum. Other phyla detected from RNA-based microbial surveys were Acidobacteria, Actinobacteria, Firmicutes, Nitrospirae and Gemmatimonadetes. The detected metabolically active micro-organisms represented only a fraction of the total bacterial community present in the studied colonizations as compared from DGGE analysis. CONCLUSIONS: The major bacterial participants in the development of yellow and grey colonizations in Altamira Cave were determined using RNA-based molecular techniques. Micro-organisms showing undetectable activity represent a potential risk for the conservation of these paintings if environmental conditions experience variations. SIGNIFICANCE AND IMPACT OF THE STUDY: Caves with palaeolithic paintings are affected by microbial deterioration. Assessing the composition of the microbial communities colonizing these caves represents a first stage to understand and control these colonizations.


Asunto(s)
Bacterias/genética , Espacios Confinados , Microbiología Ambiental , Pinturas , Secuencia de Bases , Dermatoglifia del ADN , ADN Bacteriano/análisis , Electroforesis en Gel de Poliacrilamida/métodos , Sedimentos Geológicos/microbiología , Datos de Secuencia Molecular , Proteobacteria/genética , ARN Ribosómico 16S/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , España
15.
J Biotechnol ; 121(4): 534-43, 2006 Feb 24.
Artículo en Inglés | MEDLINE | ID: mdl-16168509

RESUMEN

A study of the performance and microbial communities of a continuous stirred tank reactor (CSTR) treating two-phases olive mill solid wastes (OMSW) was carried out at laboratory-scale. The reactor operated at a mesophilic temperature (35 degrees C) and an influent substrate concentration of 162 g total chemical oxygen demand (COD)L(-1) and 126 g volatile solids (VS)L(-1). The data analyzed in this work corresponded to a range of organic loading rates (OLR) of between 0.75 and 3.00 g CODL(-1)d(-1), getting removal efficiencies in the range of 97.0-95.6%. Methane production rate increased from 0.164 to 0.659 L CH(4)L(reactor)(-1)d(-1) when the OLR increased within the tested range. Methane yield coefficients were 0.225 L CH(4)g(-1) COD removed and 0.290 L CH(4)g(-1) VS removed and were virtually independent of the OLR applied. A molecular characterization of the microbial communities involved in the process was also accomplished. Molecular identification of microbial species was performed by PCR amplification of 16S ribosomal RNA genes, denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. Among the predominant microorganisms in the bioreactor, the Firmicutes (mainly represented by Clostridiales) were the most abundant group, followed by the Chloroflexi and the Gamma-Proteobacteria (Pseudomonas species as the major representative). Other bacterial groups detected in the bioreactor were the Actinobacteria, Bacteroidetes and Deferribacteres. Among the Archaea, the methanogen Methanosaeta concilii was the most representative species.


Asunto(s)
Reactores Biológicos , Euryarchaeota/crecimiento & desarrollo , Bacterias Grampositivas/crecimiento & desarrollo , Eliminación de Residuos Líquidos , Anaerobiosis , Biomasa , Reactores Biológicos/microbiología , Euryarchaeota/genética , Bacterias Grampositivas/genética , Metano/biosíntesis , Metanol/metabolismo , Olea/química , ARN de Archaea/genética , ARN Bacteriano/genética , ARN Ribosómico 16S/genética
16.
An Pediatr (Barc) ; 60(2): 153-60, 2004 Feb.
Artículo en Español | MEDLINE | ID: mdl-14757020

RESUMEN

OBJECTIVE: To evaluate pulmonary arteries in patients with tetralogy of Fallot following surgery with quantified lung perfusion scintigraphy and magnetic resonance imaging. MATERIAL AND METHODS: From January 1985 to December 1999, 47 patients who underwent surgery between 1985 and 1999 were studied. To obtain values of normality, 45 infants with no pulmonary artery disease were assessed with lung perfusion scintigraphy (right lung flow: 54-61%, left lung flow: 38.7-46%) and magnetic resonance imaging axial view (right/left pulmonary artery branch diameter ratio: 1-1.1). Patients with stenosis underwent catheterization. RESULTS: Group 1: 27 patients with normal parameters. Group 2: nine patients with left pulmonary branch stenosis and irregularities in all parameters; left/right branch diameter ratio 0.51 and left lung perfusion 26.3 +/- 7.9%, r correlation ratio 65%, p < 0.005, left branch pressure gradient 34.4 +/- 17.9 mm Hg, rate-gradient r 89%, p < 0.001. Group 3: five patients with right pulmonary branch stenosis and irregularities in all parameters; right/left branch diameter ratio 0.52, reduced right lung perfusion 32 +/- 11%, with a stenotic branch gradient of 40 +/- 19 mm Hg., rate/gradient ratio r 72%, p < 0.005; gradient/perfusion ratio r 82%, p < 0.003. Group 4: Six patients with bilateral stenosis, reduced diameter in the stenotic area with normal perfusion in both lungs and bilateral gradient. Eighteen patients with stenosis underwent re-operation, with favorable outcome in 14. CONCLUSION: Magnetic resonance imaging and quantified lung perfusion scintigraphy provide useful information in the follow-up of tetralogy of Fallot.


Asunto(s)
Imagen por Resonancia Magnética , Tetralogía de Fallot/diagnóstico por imagen , Tetralogía de Fallot/patología , Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Perfusión , Arteria Pulmonar , Cintigrafía , Tetralogía de Fallot/cirugía
17.
An Sist Sanit Navar ; 37(1): 69-80, 2014.
Artículo en Español | MEDLINE | ID: mdl-24871112

RESUMEN

BACKGROUND: Parkinson's disease has a considerable impact on people's lives. It is necessary to identify the key elements that influence the process of living with Parkinson's disease so that health professionals can help patients and their relatives to live as well as possible with the changes and limitations produced by the disease. MATERIAL AND METHODS: A qualitative descriptive study was realized. This study corresponded to the first phase of a sequential, exploratory design (mixed method) that in turn included a quantitative phase. A multicentre project was carried out. Convenience sampling was applied to collect data, a semi-structured interview was realized individually with patients and carer-relatives and two questionnaires with patients: the Hoehn & Yahr scale and the PDNMS questionnaire. Content analysis of the interviews and a statistical description of the questionnaires were used. RESULTS: The sample was made up of 46 participants. Three key elements were identified in the process of living with Parkinson's disease: acceptance, adaptation and self-management. These elements conditioned the modes of living with Parkinson's disease: positive living, characterized by feelings of harmony, balance and naturalness; negative living characterized be feelings of frustration, loss of control and self-esteem. CONCLUSIONS: It is essential for health professionals to have a deep understanding of these elements, as well as of the factors that favor or hinder them. To the extent that research in this field progresses and effective interventions are identified, comprehensive patient care will be improved in consonance with the new directives for chronicity.


Asunto(s)
Cuidadores , Relaciones Familiares , Enfermedad de Parkinson , Anciano , Femenino , Humanos , Masculino , Encuestas y Cuestionarios
18.
An Sist Sanit Navar ; 36(3): 489-504, 2013.
Artículo en Español | MEDLINE | ID: mdl-24406362

RESUMEN

According to the World Health Organization, the number of people with diabetes mellitus in 2030 will be 366 millions in the whole world, with diabetes mellitus type 2 having the highest prevalence. The current National Chronicity and Diabetes Strategies advocate patients' self-management programs and/or interventions. Nevertheless, lack of knowledge and inadequate attitudes hinder the associative relationship between patients and professionals that is required in these programs. This literature review aims to show the outstanding needs of type 2 diabetes mellitus patients when it comes to managing the illness. Thus, results from this literature review could lay the foundations for the development of effective future programs, helping professionals focus on patients' needs.


Asunto(s)
Diabetes Mellitus Tipo 2/terapia , Autocuidado , Características Culturales , Necesidades y Demandas de Servicios de Salud , Humanos
19.
Mikrobiologiia ; 81(6): 720-5, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23610921

RESUMEN

Desulfovibrio vulgaris Hildenborough genome presents a phosphotransferase system putatively involved in the transport of carbohydrates. However, utilization of sugars by this sulfate-reducing bacterium has never been reported. Herein, we have observed proliferation of D. vulgaris Hildenborough with some carbohydrates, in mutualism with Stenotrophomonas maltophilia, a non-fermentative, gram-negative gammaproteobacterium, or Microbacterium, a gram-positive actinobacterium. These results suggest the importance of feedback interactions between different heterotrophic bacterial species including the alternative for D. vulgaris of exploiting additional organic resources and novel habitats. Thus, D. vulgaris strongly participates in the mineralization of carbohydrates both in complex natural and artificial systems.


Asunto(s)
Actinobacteria/metabolismo , Proteínas Bacterianas/metabolismo , Desulfovibrio vulgaris/metabolismo , Stenotrophomonas maltophilia/metabolismo , Sulfatos/metabolismo , Simbiosis/fisiología , Actinobacteria/genética , Arabinosa/metabolismo , Proteínas Bacterianas/genética , Desulfovibrio vulgaris/genética , Fructosa/metabolismo , Inositol/metabolismo , Manitol/metabolismo , Oxidación-Reducción , Stenotrophomonas maltophilia/genética , Xilosa/metabolismo
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