Adapting the three-step approach (Rossi, 1986/1993) for use with two young children.

In this study the use of The Basic Three-Step Approach for Accessing Creative Resources developed by Ernest L. Rossi (1986/1993) has been investigated for its adaptability for use with children for the resolution of stress related mind-body problems. The methodology utilizes ideodynamic processes, accessing questions, implied directives, and the therapeutic double bind. The two children in this study are 4- and 9-year-old girls. The results suggest that this therapeutic approach can be successfully adapted for use with young children and is worthy of continuing research.

The diagnosis of pregnancy in sheep by abdominal palpation.

Pregnancy diagnosis in Merino ewes by abdominal palpation was 80 to 95 percent accurate in ewes 90 to 130 days pregnant. The ease with which the foetus could be palpated increased with increasing gestational age. Animals in store body condition were more easily diagnosed than those in forward store condition. Withholding feed and water for 12 to 24 hours increased the ease of diagnosis. One operator and two assistants could perform the technique at a rate of 150 to 200 sheep per hour. The animals were held in the sitting position and no special restraining apparatus was necessary. The potential for using this technique may be associated with investigations of reproductive wastage or the selection of pregnant animals for preferential management during drought conditions.

In vitro growth of ovine squamous cell carcinoma.

Cultured by fragment explantation following a selective trypsinisation procedure, 22/31 (71%) specimens of ovine squamous cell carcinoma yielded pure strains of epithelial cells which to date have passaged vigorously to the 29th level of passage. In contrast to the diploid nature of epithelial cell strains derived from foetal sheep skin, the cell strains derived from skin tumors were highly aneuploid.

Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima.

The 1,860,725-base-pair genome of Thermotoga maritima MSB8 contains 1,877 predicted coding regions, 1,014 (54%) of which have functional assignments and 863 (46%) of which are of unknown function. Genome analysis reveals numerous pathways involved in degradation of sugars and plant polysaccharides, and 108 genes that have orthologues only in the genomes of other thermophilic Eubacteria and Archaea. Of the Eubacteria sequenced to date, T. maritima has the highest percentage (24%) of genes that are most similar to archaeal genes. Eighty-one archaeal-like genes are clustered in 15 regions of the T. maritima genome that range in size from 4 to 20 kilobases. Conservation of gene order between T. maritima and Archaea in many of the clustered regions suggests that lateral gene transfer may have occurred between thermophilic Eubacteria and Archaea.