RESUMEN
The ECL gene detection system is a novel, sensitive, non-radioactive system for the detection of nucleic acid hybridized on both nylon and nitrocellulose membranes. It is characterized by direct labeling of probe sequences with horseradish peroxidase combined with an enhanced chemiluminescent (ECL) detection reaction; the light output is captured on blue-light sensitive film. The application of the system to a range of standard molecular biology hybridization techniques is described.
Asunto(s)
Mediciones Luminiscentes , Ácidos Nucleicos/análisis , Northern Blotting , Southern Blotting , Genes ras , Peroxidasa de Rábano Silvestre , Mieloma Múltiple/genética , Hibridación de Ácido Nucleico , Sondas de Ácido NucleicoRESUMEN
This article is predominantly concerned with the value of single-case research designs for generating nursing knowledge. The argument in favour of single-case research designs is made with reference to the testing of a range of body-worn incontinence pads in a group of cognitively impaired elderly women in two clinical settings.