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The copper phytate IP6Cu, IP6Cu2 and IP6Cu3 complexes were synthesized changing the phytate to metal mole ratio. The obtained products have been characterized by means of chemical and spectroscopic studies. Spectroscopic ATR/IR, FT-Raman, UV-Vis, EPR and magnetic measurements were carried out. The structures of these compounds have been proposed on the basis of the group theory and geometry optimization taking into account the shape and number of the bands corresponding to the stretching and bending vibrations of the phosphate group and metal-oxygen polyhedron. The role of the inter- and intra-hydrogen bonds in stabilization of the structure has been discussed. EPR studies showed that a local rhombic symmetry of copper ions appears in the studied phytates. Dominant interactions show antiferromagnetic properties depending on the content of paramagnetic ions.
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Complejos de Coordinación/química , Cobre/química , Ácido Fítico/química , Teoría Funcional de la Densidad , Espectroscopía de Resonancia por Spin del Electrón , Modelos Moleculares , Conformación Molecular , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Espectrometría RamanRESUMEN
In this work, for the first time, detailed structural and optical characterization of RbLaP4O12 doped with different concentrations of Ce3+, Nd3+, Tm3+, or Yb3+ ions is reported. The samples were obtained via a precipitation technique. Their structural characterization was performed using X-ray diffraction (XRD), and infrared and Raman spectroscopies. Following XRD data, the unit cell parameters of host lattices were calculated using Rietveld refinement. It was found that an increase in the dopant content leads to a decrease in the unit cell volume. The optical characterization of RbLaP4O12:Ln3+ was carried out by collecting absorption and emission spectra, as well as luminescence decay profiles. Following absorption spectra, the energy band gap of the studied matrix was determined. It was found that the broad absorption band located in the ultra-violet range, in most cases ascribed to charge transfer or f-d transitions, is in fact related to the absorption of the host lattice. The analysis of luminescence properties allowed us to investigate possible ways of depopulation emission levels of impurities.
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We report the synthesis, crystal structure, IR, Raman and luminescence studies of four metal-organic frameworks of the following formulas: [CH3CH2NH3]Y1-x-yYbyErx(HCOO)4 (x = 1, y = 0; x = 0.2, y = 0.8; x = 0.02, y = 0.07) and [CH3CH2NH3]Y0.92Eu0.08(HCOO)4. All the compounds are isostructural and crystallize in a polar and non-centrosymmetric monoclinic system (P21 space group). They have been characterized by single crystal and powder X-ray diffraction methods as well as by vibrational spectroscopy (IR and Raman). The assignment of the external and internal modes has been discussed and presented. Furthermore, the optical properties of the Er3+ and Eu3+ ions have been assessed using diffuse absorption, excitation and emission spectra. The site symmetry of the Eu3+ ions has been analyzed using the emission spectrum and the luminescence decay of the red emission.
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The structural and spectroscopic properties of Sc2(MoO4)3 molybdate containing various concentrations of Cr3+ ions were investigated in a temperature range of 80-300 K. The samples were prepared using hydrothermal as well as solid-state reaction methods. The influence of synthesis conditions and the molybdenum source on the structural properties was studied by X-ray diffraction (XRD), IR (infrared), and Raman methods. The optical properties of Sc2(MoO4)3 samples doped with 0.1, 0.5, 1.0, and 2.0 % of Cr3+ ions were investigated. The broadband near-infrared (NIR) luminescence spectra generated from the 4T2 and 2E levels of Cr3+ ions may be attractive for NIR light-emitting diode (LED) applications. Emission decay profiles and the crystal field parameters of Cr3+ ions are discussed. In particular, the mechanism of photoluminescence generation and the thermal quenching path are described in detail.
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We report density functional theory (DFT) studies of vibrational modes for benzyltrimethylammonium cations (BeTriMe+) as well as THz, IR and Raman studies of [BeTriMe][M(dca)3(H2O)] (dca = N(CN)2-, dicyanamide; M = Mn2+, Co2+, Ni2+) and their anhydrous analogues. These studies show that the anhydrous BeTriMeMn and BeTriMeNi have the same or very similar structures and loss of water molecules leads to significant changes in the metal-dicyanamide frameworks. In particular, the number of dca modes decreases, suggesting increase of crystal symmetry, probablly related with decrease in the number of non-equivalent dca bridges from two to one. Although it is possible that dehydration leads to a replacement of the coordinate Mn-O (Ni-O) bonds by Mn-N (Ni-N) bonds, wherein N atoms come from the C≡N groups of previously non-bridged dca units, reversibility of the dehydration process indicates that such new bonds are either not formed or are very weak. The anhydrous Mn and Ni compounds undergo similar reversible phase transitions to lower symmetry phases. The driving force for these transitions is most likely ordering of dca linkers but this process is accompanied by weak distortion of the metal-dicyanamide frameworks. In the case of BeTriMeCo, the loss of water molecules also leads to significant changes in the cobalt-dicyanamide framework. However, the structure of this analogue is different from the structures of the Mn and Ni counterparts, the number of unique dca linkers is preserved and the dehydration process is irreversible, suggesting more drastic rearrangement of the metal-dicynamide framework.
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The detailed temperature-dependent IR and Raman spectra were used to study and understand the mechanism of structural phase transition occurring at 175 K in manganese hypophosphite templated with formamidinium (FA+) ions, [FA]Mn(H2POO)3, which adopts a perovskite-like architecture. The structural transformation between the C2/c and the P21/c monoclinic phases has a complicated nature and is mainly driven by re-orientational motions of the FA+ cations but it is also accompanied by a significant distortion of the MnO6 octahedral units as well as steric-forced changes of the PH2 groups determining the off-center shifts of FA+ cations in the cages. The re-orientational motions of formamidinium cations at 175 K are followed by slight changes of their geometry and re-arrangement of hydrogen bonds (HBs). The strong temperature-dependences of bands corresponding to vibrations involving hydrogen bonding reveal the highly-dynamic character of this phase transition and strong nature of created HBs. The most pronounced changes are observed for the modes corresponding to the formamidinium cation, proving that the phase transition has an order-disorder character.
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Methyl groups are ubiquitous in synthetic materials and biomolecules. At sufficiently low temperature, they behave as quantum rotors and populate only the rotational ground state. In a symmetric potential, the three localized substates are degenerate and become mixed by the tunnel overlap to delocalized states separated by the tunnel splitting ν t . Although ν t can be inferred by several techniques, coherent superposition of the tunnel-split states and direct measurement of ν t have proven elusive. Here, we show that a nearby electron spin provides a handle on the tunnel transition, allowing for its excitation and readout. Unlike existing dynamical nuclear polarization techniques, our experiment transfers polarization from the electron spin to methyl proton spins with an efficiency that is independent of the magnetic field and does not rely on an unusually large tunnel splitting. Our results also demonstrate control of quantum states despite the lack of an associated transition dipole moment.
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The temperature-dependent IR and Raman spectroscopy has been used to study the phase transitions in manganese-azide frameworks with either dimethylammonium (DMA+) or tetramethylammonium (TMA+) cations which adopt a perovskite-like crystal structure. The phase transition in DMA-analogue seems to be associated with cooperative tilting of MnN6 octahedra and order-disorder of hydrogen bonds while in TMA-analogue it is more complex and composed of several processes, including the motions of both manganese-azide framework and tetramethylammonium cations and their possible coupling. Our results are in agreement with the data received from crystallographic and dielectric measurements in the case of TMA-analogue and we have demonstrated the importance of order-disorder of hydrogen bonds in the case of DMA-analogue which previously has not been taken into account.
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BACKGROUND: One of the most severe traumatic brain injuries, the subdural haematoma, is related to damage and rupture of the bridging veins, generating an abnormal collection of blood between the dura mater and arachnoid mater. Current numerical models of these vessels rely on very simple geometries and material laws, limiting its accuracy and bio-fidelity. METHODS: In this work, departing from an existing human head numerical model, a realistic geometry for the bridging veins was developed, devoting special attention to the finite elements type employed. A novel and adequate constitutive model including damage behavior was also successfully implemented. FINDINGS: Results attest that vessel tearing onset was correctly captured, after comparison against experiments on cadavers. INTERPRETATION: Doing so, the model allow to precisely predict the individual influence of kinematic parameters such as the pulse duration, linear and rotational accelerations in promoting vessel tearing.
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Hematoma Subdural/diagnóstico , Rotura/diagnóstico , Aceleración , Fenómenos Biomecánicos , Cadáver , Simulación por Computador , Elasticidad , Femenino , Análisis de Elementos Finitos , Cabeza/fisiopatología , Hematoma Subdural/fisiopatología , Humanos , Masculino , Modelos Teóricos , Rotura/fisiopatologíaRESUMEN
Eu3+ complex with 1-(2,6-dihydroxyphenyl)ethanone in the solid state has been synthesized and characterized by elemental analysis, UV-visible, FT-IR and FT-Raman spectroscopies, powder X-ray diffraction, electron emission under femtosecond laser excitation. The stoichiometry and the formula of the studied complex have been proposed. Its physicochemical properties have been analyzed in terms of the structure and DFT calculations performed for the ligand. The luminescence and dynamics of the excited states depopulation have been studied using femtosecond laser excitation. Spectral and energetic transformation of femtosecond light impulses has been studied and possibility of the energy transfer between the ligand and the Eu3+ electron levels has been analyzed.
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Metal-organic frameworks (MOFs), in which metal clusters are coupled by organic moieties, exhibit inherent porosity and crystallinity. Although these systems have been examined for vast potential applications, the elementary proton conduction in anhydrous MOFs still remains elusive. One of the approaches to deal with this problem is the utilization of protic organic molecules, to be accommodated in the porous framework. In this work we report the temperature-dependent crystal structure and proton conduction in [C2H5NH3][Na0.5Fe0.5(HCOO)3] metal-organic frameworks using X-ray diffraction and broadband dielectric spectroscopic techniques. The detailed analysis of the crystal structure reveals disorder of the terminal ethylene groups in the polar phase (space group Pn). The structural phase transition from Pn to P21/n at T ≈ 363 K involves the distortion of the metal formate framework and ordering of EtA+ cations due to the reduction of the cell volume. The dielectric data have been presented in the dynamic window of permittivity formalism to understand the ferroelectric phase transition. The relaxation times have been estimated from the Kramers-Kronig transformation of the dielectric permittivity. A Grotthuss type mechanism of the proton conduction is possible at low temperatures with the activation energy of 0.23 eV. This type of experimental observation is expected to provide new prospective on the fundamental aspect of elementary proton transfer in anhydrous MOFs.
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BACKGROUND: Insect defensin A is a basic 4 kDa protein secreted by Phormia terranovae larvae in response to bacterial challenges or injuries. Previous biological tests suggest that the bacterial cytoplasmic membrane is the target of defensin A. The structural study of this protein is the first step towards establishing a structure-activity relationship and forms the basis for understanding its antibiotic activity at the molecular level. RESULTS: We describe a refined model of the three-dimensional structure of defensin A derived from an extensive analysis of 786 inter-proton nuclear Overhauser effects. The backbone fold involves an N-terminal loop and an alpha-helical fragment followed by an antiparallel beta-structure. The helix and the beta-structure are connected by two of the three disulphide bridges present in defensin A, forming a so-called 'cysteine-stabilized alpha beta' (CS alpha beta) motif. The N-terminal loop, which is locally well defined, can occupy different positions with respect to the other moieties of the molecule. CONCLUSIONS: The CS alpha beta motif, which forms the core of the defensin A structure, appears to be a common organization for several families of small proteins with toxic properties. The distribution of amino acid side chains in the protein structure creates several hydrophobic or hydrophilic patches. This leads us to propose that the initial step in the action of positively charged defensin A molecules with cytoplasmic membranes may involve interactions with acidic phospholipids.
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Defensinas , Hormonas de Insectos/química , Modelos Moleculares , Conformación Proteica , Secuencia de Aminoácidos , Animales , Bacteriólisis , Fenómenos Químicos , Química Física , Dípteros/química , Bacterias Grampositivas/efectos de los fármacos , Enlace de Hidrógeno , Hormonas de Insectos/farmacología , Espectroscopía de Resonancia Magnética , Datos de Secuencia Molecular , Proteínas Recombinantes/química , Homología de Secuencia de Aminoácido , Soluciones , Relación Estructura-ActividadRESUMEN
The interactions between the antifungal lipopeptide mycosubtilin and lipids are studied. Mycosubtilin increases the ion permeability of planar lipid membranes by forming ion conducting pores. The lifetime of these pores is greatly increased when the membrane contains cholesterol. In mixed monolayers the interaction between mycosubtilin and DMPC leads to the formation of a mycosubtilin/DMPC 1:2 complex non miscible in the excess DMPC monolayer but miscible in the mycosubtilin monolayer. Mycosubtilin and cholesterol interact strongly in monolayers in all proportions and form a mycosubtilin-cholesterol (1:2) complex. These results are analyzed with reference to the overall view of the activity of iturins and the importance of the lipopeptide conformation is outlined.
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Lípidos de la Membrana , Secuencia de Aminoácidos , Permeabilidad de la Membrana Celular , Colesterol , Lipoproteínas , Datos de Secuencia Molecular , Conformación Proteica , TermodinámicaRESUMEN
The A21978C group are lipopeptide antibiotics which kill Gram-positive bacteria only in the presence of calcium ions. The calcium requirement of the antibacterial activity of A21978C correlates well with an in vitro calcium-dependent insertion into phospholipid vesicles. In this paper the interaction of A21978C with phosphatidylcholine is investigated in mixed monomolecular films. The spontaneity of the antibiotic-lipid mixing was determined by calculating the free energy change. On a Ca2+ containing subphase there is a specific interaction between the components at all antibiotic-lipid ratios. This is not true on K+ subphases, where specific interactions never occur. On Mg2+ subphases specific interactions occur only in monolayers containing very little lipid. By analysing the fluorescence of the kynurenine residue we have followed the effects of two factors on the penetration of the antibiotic into lipid bilayer vesicles. Firstly, the phospholipid gel to liquid crystalline phase transition which in the absence of calcium leads to an exclusion of the antibiotic from the bilayer. This trend is completely reversed in the presence of Ca2+. Secondly, the role of this lipopeptide's lipid tail was clarified by use of a series of versions of increasing fatty acyl chain length. The results indicate that the interaction promoted by calcium is not simply a hydrophobic attraction between fatty acyl chains but is more likely to be a specific interaction between polar headgroups.
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Antibacterianos , Calcio/metabolismo , Dimiristoilfosfatidilcolina/metabolismo , Péptidos , Interacciones Farmacológicas , Péptidos y Proteínas de Señalización Intercelular , Lípidos de la Membrana/metabolismo , Péptidos Cíclicos/farmacología , Fosfolípidos/metabolismo , Espectrometría de Fluorescencia , TemperaturaRESUMEN
Stearic acids with a nitroxide radical at selected positions have been incorporated in the phospholipid bilayers of clathrin coated vesicles, uncoated vesicles and sonicated liposomes made from the lipids extracted from the uncoated vesicles. The extent of incorporation was found minimum for stearic acids labeled on C-12 and for bilayers of uncoated vesicles. The ESR spectra of the spin-labeled fatty acids incorporated in the bilayers showed a pronounced temperature dependence (without discontinuity) and a decrease in the hyperfine splitting as the nitroxide group was inserted deeper in the hydrophobic core of the membranes. An abrupt phospholipid phase transition or a phase separation could be excluded. The presence of the external proteins (the clathrin coat) on the membranes was not found to noticeably influence the gradient of flexibility of the fatty acid chains of the phospholipids. The influence of the internal proteins embedded in the bilayers was evidenced by a detailed analysis of the ESR spectra of (7,8)SA in terms of two components: one component arising from the labels surrounded exclusively by phospholipids, the other component arising from labels of reduced mobility perturbed by the vicinity of the proteins. These results support the persistence of lipidic domains in the endocytic vesicles despite the accumulation of receptors which follows their formation.
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Encéfalo/metabolismo , Clatrina/metabolismo , Óxidos N-Cíclicos/metabolismo , Marcadores de Spin , Animales , Bovinos , Invaginaciones Cubiertas de la Membrana Celular/metabolismo , Espectroscopía de Resonancia por Spin del Electrón , Membrana Dobles de Lípidos/metabolismo , Liposomas/metabolismo , Proteínas de la Membrana/metabolismo , Concentración Osmolar , Fosfolípidos/metabolismo , Ácidos Esteáricos/metabolismo , TemperaturaRESUMEN
A21978C is a calcium-dependent lipopeptide antibiotic whose biological properties are modulated by changes in its lipid chain length. This article reports on the monolayer characteristics of this cyclic lipopeptide and of LY146032 a semi synthetic homologue. The equilibrium spreading pressure pi e increases linearly with the ionic concentration of the subphase and is higher with divalent cations. The nature of the divalent cation plays a crucial role in the spreading as indicated by the variation in the molecular free energy delta Gs.delta Gs decreases in the order K+ greater than Mg2+ greater than Ca2+, which indicates privileged interactions with Ca2+. Also, the larger the lipid chain, the easier the spreading of antibiotic molecules. The compression isotherm curves are shown. The mean area of the uncompressed molecules is around 220-240 A 2 which is compatible with the size of the peptide cycle lying at the interface. The isotherm curves of the natural compounds show a transition region where the molecules are more compressible. At a given area/molecule, the surface pressures increase with the acyl chain length. When the molecules are spread on various salt solutions, the surface pressures increase in the order K+ less than Mg2+ less than Ca2+. The isotherm curves are not reversible upon a compression-expansion cycle and a wide amplitude hysteresis is observed. If a second compression is done, the curve shape is that of a liquid-expanded state and the transition region is no longer observed. This implies a conformational change of the molecules during the first compression process.
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Antibacterianos , Daptomicina , Péptidos y Proteínas de Señalización Intercelular , Lipoproteínas , Péptidos , Péptidos Cíclicos , Conformación Proteica , Soluciones , Relación Estructura-Actividad , TermodinámicaRESUMEN
The miscibility and the interactions of the antifungal lipopeptide iturin A with lipids, DMPC and cholesterol, are studied in monolayers at the air/water interface and a comparison of the respective behaviour of iturin A and the biologically inactive methylated derivative MeTyr-iturin A is made. Each lipopeptide is miscible with anyone of the lipids. This behaviour is revealed by the dependence of the transition pressure upon composition and by deviations from the additivity rule of the mean molecular area. The thermodynamic properties of the mixed systems are studied by the method of Goodrich. The mixed monolayers are always more stable than the two separate components, subsequently there are interactions between the components. However, the excess free energy of mixing delta Gexm is positive for the iturin A/DMPC system which is an indication that the interactions between lipopeptide and lipid molecules are weaker than the interactions between the pure components themselves. This is compatible with the presence of self-associated lipopeptide molecules. However, delta Gexm is highly negative for the iturin A/cholesterol system giving evidence of the formation of a specific complex between iturin A and cholesterol which is not the case with the methylated derivative. These data are analysed in connection with previous results concerning the pore-forming properties of these lipopeptides and the lack of biological activity of MeTyr-iturin A.
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Antibacterianos , Lípidos de la Membrana , Péptidos , Colesterol , Dimiristoilfosfatidilcolina , Metilación , Péptidos Cíclicos , Solubilidad , Relación Estructura-Actividad , TermodinámicaRESUMEN
A comparison has been made between the pore-forming properties of the antibiotic lipopeptide iturin A and a derivative methylated on the tyrosine residue which shows a restricted biological activity. It is shown that this derivative increases the ion permeability of planar lipid membranes as does iturin A. Nevertheless, the global conductance of the doped membrane is very much lower at the same lipopeptide/phospholipid ratio and the ion selectivity is inverted (PK/PCl = 6 instead of 0.6 with iturin A). The characteristics of the induced conducting pores are also rather different. This suggests an important role of the D-Tyr2 residue, present in all the compounds of the iturin family, both in the biological and in the pore-forming properties of iturin A.
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Antibacterianos , Péptidos , Tirosina , Colesterol , Liposomas , Potenciales de la Membrana , Metilación , Modelos Biológicos , Péptidos Cíclicos , FosfatidilcolinasRESUMEN
The binding of clathrin and accessory coat proteins to small unilamellar vesicles and to liposomes of uncharged phospholipids has been followed by chromatography, 31P-NMR, ESR and fluorescence anisotropy. At pH 6.5 and at an ionic strength value (0.1 M Mes) close to that used during the purification of clathrin-coated vesicles, the proteins do not restore the characteristic network found around the natural vesicles. Instead, a limited fusion leads to enlarged structures in which the perturbation of the dynamics of the phospholipids decreases gradually with the depth in the membrane. While the rate of motion of the outer polar heads is lowered, the order parameter of doxyl groups located either under or in the vicinity of the glycerol backbone is not affected by the proteins. In the inner core of the membrane, the main thermotropic transition of the hydrocarbon chains is unchanged. All the effects are the results of interactions limited to the membrane surface. The electrostatic nature of these interactions is evidenced when the embedded spin labels have a charge protruding at the membrane surface. An 'anchoring' effect appears which is due to the charged groups of the proteins. The lateral diffusion of the probes is reduced and, at low ionic strength, a cationic derivative no longer detects the thermotropic transition of the hydrocarbon chains. These results indicate that, although it is known that clathrin and accessory proteins bind to membranes by a series of protein-protein interactions, this system is not devoid of lipid-protein interactions, at least when it is not organized as in the natural system.
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Clatrina/metabolismo , Liposomas/metabolismo , Fosfatidilcolinas/metabolismo , Fosfoproteínas/metabolismo , Proteínas Adaptadoras del Transporte Vesicular , Animales , Encéfalo/ultraestructura , Bovinos , Cromatografía , Invaginaciones Cubiertas de la Membrana Celular/análisis , Óxidos N-Cíclicos , Difenilhexatrieno , Espectroscopía de Resonancia por Spin del Electrón , Polarización de Fluorescencia , Colorantes Fluorescentes , Concentración de Iones de Hidrógeno , Espectroscopía de Resonancia Magnética , Microscopía Electrónica , Concentración Osmolar , Marcadores de Spin , TemperaturaRESUMEN
The ionization of fatty acids, fatty amines and N-acylamino acids incorporated in phosphatidylcholine single-walled vesicles has been measured. The guest molecules have been specifically enriched with 13C and titrated by using NMR spectroscopy. The apparent pKa of fatty acids in phosphatidylcholine bilayers if 7.2-7.4 and those of fatty amines are approx. 9.5. These pKa values depend on many different parameters related to the structure of the lipid/solution interface, to the composition of the aqueous medium and to the localization of the ionizable groups. A special sensitivity to the ionic strength and to the surface charge has been found. A positive surface charge decreases the pKa value whereas a negative one increases it, the total range of variation being 2.5-3 units. In a qualitative macroscopic interpretation, it is proposed that pKa is essentially determined by the low polarity of the lipidic matrix.