RESUMEN
Conosiligins A-D (1-4), four ring-rearranged sesquiterpenoids, were isolated from cultures of the basidiomycete Conocybe siliginea. Their structures and absolute configurations were determined by detailed spectroscopic analyses and equivalent circulating density (ECD) calculations. Compounds 1 and 2 possess a 5/8-fused ring system, while 3 has a 5/6-fused backbone conjugated with a γ-lactone. Compound 4 is a 5,6-seco tremulane derivative with the loss of a skeletal carbon, featuring a tetracyclic system involving a pyranone moiety. Compounds 3 and 4 inhibited Con A-induced T cell proliferation with IC50 values of 12.3 and 6.6 µM, respectively.
Asunto(s)
Agaricales/química , Inmunosupresores/química , Inmunosupresores/farmacología , Sesquiterpenos/química , Animales , Linfocitos B/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Femenino , Ratones , Ratones Endogámicos BALB C , Estructura Molecular , Linfocitos T/efectos de los fármacosRESUMEN
Three newly isolated ergosterols, psathergosterols A-C (1-3), together with two known ones (4 and 5), have been isolated from cultures of the basdiomycete Psathyrella candolleana. Their structures with the absolute configuration were elucidated by means of spectroscopic methods and the single crystal X-ray diffraction. Compounds 2-4 exhibited certain cytotoxicities to five human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7, SW480).
Asunto(s)
Antineoplásicos/farmacología , Basidiomycota/química , Ergosterol/farmacología , Antineoplásicos/aislamiento & purificación , Línea Celular Tumoral , China , Ensayos de Selección de Medicamentos Antitumorales , Ergosterol/aislamiento & purificación , Humanos , Estructura MolecularRESUMEN
Two highly oxygenated ergosterols, (22E,24R)-3ß,5α,6ß,9α,14α,25-hexhydroxyergosta-7,22-diene (1), and (22E,24R)-3ß,5α,6ß,14α,25-pentahydroxyergosta-7,9(11),22-triene (2), together with two known ones, have been isolated from cultures of the basidiomycete Conocybe siliginea. Their structures were elucidated on the basis of extensive spectroscopic means. Two new compounds (1 and 2) were tested for their nitric oxide synthase inhibitory activities. However, none of them showed any activity (IC50 > 40 µM).