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1.
Arch Virol ; 158(6): 1349-53, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23361863

RESUMEN

A begomovirus and its associated alpha- and betasatellite were detected in tomato plants affected with leaf curl disease. Based on a nucleotide sequence identity of 99 %, this begomovirus was designated an isolate of cotton leaf curl Burewala virus (CLCuBuV). The alphasatellite exhibited 93 % sequence identity to cotton leaf curl Burewala alphasatellite (CLCuBuA) and is hence referred to here as a variant of CLCuBuA. The detected betasatellite was recombinant in nature and showed 70 % sequence identity to the known betasatellites. Inoculation of healthy tomato with CLCuBuV plus betasatellite, either in the presence or the absence of alphasatellite, led to typical leaf curling, while inoculation with CLCuBuV in the absence of betasatellite resulted in mild symptoms. This confirmed the role of the betasatellite in expression of disease symptoms. We propose to name the newly detected betasatellite tomato leaf curl Hajipur betasatellite (ToLCHJB).


Asunto(s)
Begomovirus/genética , ADN Satélite/genética , Enfermedades de las Plantas/virología , Hojas de la Planta/virología , Solanum lycopersicum/virología , Secuencia de Bases , Gossypium/virología , India , Datos de Secuencia Molecular , Filogenia
2.
Cytojournal ; 6: 13, 2009 Jul 18.
Artículo en Inglés | MEDLINE | ID: mdl-19680441

RESUMEN

OBJECTIVES: The objectives were to correlate the modified fluorescent method with the conventional Ziehl-Neelsen (ZN) method for the detection of acid-fast bacilli (AFB) and, also to study the efficacy and advantages of using the auramine-rhodamine stain on lymph node aspirates under fluorescent microscopy. METHODS: In 108 consecutive patients with a clinical suspicion of tuberculosis (TB) presenting with lymphadenopathy, fine needle aspirations were performed. Smears from the aspirates were processed for routine cytology, the conventional ZN method, and the modified fluorescent method. The significance of the modified fluorescent method over the conventional ZN method was analyzed using the chi-square test. RESULTS: Out of 108 aspirates, 102 were studied and remaining 6 were excluded from the study due to diagnosis of malignancy in 4.04% (4/6) and inadequate aspiration in 2.02% (2/6). Among the 102 aspirates, 44.11% (45/102) were positive for AFB on the conventional ZN method, 58.9% (60/102) were indicative of TB on cytology, while the smear positive increased to 81.37% (83/102) on the modified fluorescent method. CONCLUSIONS: Fluorescent microscopy has the advantage of speed and ease of screening, and reduces observer fatigue. The modified fluorescent method was found to be more advantageous than routine cytology and conventional ZN method, particularly in paucibacillary cases. The bacillary positivity rates were higher in the modified fluorescent method than in the ZN method. Hence, the modified fluorescent method can be an adjuvant when used with routine cytology for the identification of AFB.

3.
Acta Cytol ; 53(2): 174-8, 2009.
Artículo en Inglés | MEDLINE | ID: mdl-19365970

RESUMEN

OBJECTIVE: To emphasize the role of fine needle aspiration cytology (FNAC) in etiologic workup in significant cervical lymphadenopathy in children. STUDY DESIGN: A total of 336 consecutive children, aged 1 month to 12 years, with significant cervical lymphadenopathy were subjected to FNAC. Children on empirical/specific therapy for lymphadenopathy for >2 months and children with lymphadenopathy other than cervical region were excluded from this study. RESULTS: Of 324 cases, the cytomorphologic features observed were reactive lymphadenitis in 58.02% of cases, granulomatous lymphadenitis in 30.55%, suppurative lymphadenitis in 7.10% and malignancies in 5.62%. The remaining 3. 57% of cases were excluded because of inadequate aspiration. The most common cause diagnosed was tuberculosis in 29.01% of cases followed by chronic tonsillopharyngitis in 28.39%, suppurative lymphadenitis in 7.10%, human immune deficiency infection in 5.55% and malignancies in CONCLUSION: FNAC is an important diagnostic modality for the etiologic workup in significant cervical lymphadenopathy in children. It is almost as sensitive and specific as excision lymph node biopsy when an adequate aspirate is examined by expert eyes.


Asunto(s)
Biopsia con Aguja Fina , Enfermedades Linfáticas/etiología , Enfermedades Linfáticas/patología , Niño , Preescolar , Femenino , Infecciones por VIH/complicaciones , Humanos , Lactante , Enfermedades Linfáticas/cirugía , Masculino , Cuello/patología , Neoplasias/complicaciones , Faringitis/complicaciones , Tonsilitis/complicaciones , Tuberculosis Ganglionar/complicaciones
4.
Indian J Pathol Microbiol ; 50(2): 367-9, 2007 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-17883076

RESUMEN

A 36-year-old man presented with history suggestive of intracranial space occupying lesion. Computed tomography of brain revealed a large lobulated, extra axial, hyperdense lesion in the right fronto-temporal region extending up to to the right frontal and ethmoidal sinuses, eroding the bone, enhancing homogeneously with contrast, which was suggestive of atypical meningioma. He had no predisposing factors that could lead to the suspicion of opportunistic infection. Craniotomy and total excision of the lesion was done. Histopathological study revealed aspergilloma.


Asunto(s)
Aspergilosis/diagnóstico , Neoplasias Encefálicas/diagnóstico , Meningioma/diagnóstico , Meningitis Fúngica/diagnóstico , Adulto , Aspergilosis/patología , Diagnóstico Diferencial , Humanos , Inmunocompetencia , Masculino , Meningitis Fúngica/patología , Enfermedades de los Senos Paranasales/diagnóstico , Enfermedades de los Senos Paranasales/patología , Tomografía Computarizada por Rayos X
5.
Indian J Otolaryngol Head Neck Surg ; 69(1): 62-66, 2017 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-28239581

RESUMEN

Atrophic rhinitis (AR) is a chronic debilitating nasal mucosal disease of unknown aetiology and the definitive treatment is still elusive. This often leads to the patient suffering during the entire life span often depleting the individual psychologically. On the contrary, Rhinoscleroma (RS) for which the aetiology is clearly known and is completely curable has atrophic stage which is clinically indistinguishable from AR. Many cases of atrophic stage of RS are undetected and often they end up being treated as AR. This study was conducted to know the role of histopathology and other factors in differentiating AR and Atrophic stage of RS, which can significantly alter the course of treatment and outcome. Forty-five cases of clinically diagnosed AR were included in the study. Punch biopsy of nasal mucosa was obtained from the anterior end of inferior turbinate. Core culture was performed on one sample and histopathological examination for the other sample. Among the 45 patients, 38 (84.44%) of cases were diagnosed to be AR and 7 (15.56%) cases were diagnosed to be RS by histopathology. Core culture of RS subjects showed positive culture for Klebsiella rhinoscleromatis in five subjects (71%). To conclude, AR cases should be confirmed by histopathological examination to rule out RS, for effective management and to prevent complications.

6.
Saudi J Gastroenterol ; 17(2): 110-3, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21372347

RESUMEN

BACKGROUND/AIM: Intestinal tuberculosis needs to be considered in the differential diagnosis when patients with intestinal pathology are encountered. Tuberculosis can mimic other disease entities like, ischemic enteritis, inflammatory bowel diseases, malignancies, intussusception etc., clinically as well as morphologically in resected intestinal specimens. We aimed to study the various clinical presentations leading to intestinal resection, with identification of different etiological factors by histopathological examination; and to illustrate, discuss and describe the various histopathological features of the lesions in these resected intestinal specimens with clinicopathological correlation. MATERIALS AND METHODS: We studied 100 cases of resected intestinal specimens received during September 2002 to December 2003. We totally encountered 22 request forms with clinical suspicion of ileoceocal tuberculosis. RESULTS: Abdominal tenderness and mass in ileoceocal region were noted in all cases. In many instances, the cases were operated for acute/subacute intestinal obstruction. Clinical and intra-operative diagnoses of tubercular enteritis, in many instances, were finally diagnosed histopathologically as ischemic enteritis (nine cases), chronic nonspecific enteritis (four cases), adenocarcinoma of the caecum, Crohn's disease, intussusception (each one case), and correctly as intestinal tuberculosis in only six cases. CONCLUSION: Tuberculosis can mimic various disease entities, clinically and sometimes morphologically. Vice versa is also true. An increased awareness of intestinal tuberculosis coupled with varied clinical presentations, nonspecific signs and symptoms, difficulties in diagnostic methods and need of early and specific treatment should improve the outcome for patients with this disease.


Asunto(s)
Enfermedades del Ciego/microbiología , Enfermedades del Ciego/patología , Enfermedades del Íleon/microbiología , Enfermedades del Íleon/patología , Tuberculosis Gastrointestinal/diagnóstico , Enfermedades del Ciego/terapia , Estudios de Cohortes , Diagnóstico Diferencial , Humanos , Enfermedades del Íleon/terapia , Estudios Retrospectivos , Tuberculosis Gastrointestinal/terapia
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