RESUMEN
The decrease in titer of PRV antibodies in serum was evaluated at 10, 37, 67, 109 and 173 days of age in 16 non-vaccinated pigs and 43 pigs vaccinated at 3, 67 and 80 days of age with a modified live TK/gIII gene deleted pseudorabies virus (PRV) vaccine. Serum samples were analyzed for antibodies to PRV by the serum-virus neutralization test (SN), a commercial competitive ELISA (CELISA), and the CELISA OMNIMARK PRV differential (OMD) diagnostic kit. At 10 days of age, all pigs had SN titers > or = 1:4 and were CELISA+/OMD+, indicating circulating antibodies to field strains of PRV. At 109 days, all non-vaccinated pigs had SN titers < 1:4. Forty-five percent of vaccinated pigs had SN titers > or = 1:4, 56% were CELISA positive and most were CELISA+/OMD-, indicating antibodies due to vaccination. At 24 weeks of age, all pigs had SN titers > or = 1:4 and were CELISA+/OMD+ due to exposure to field strains. Although circulating maternal antibodies interfere with the development of active immunity, vaccination at 3 days of age resulted in detectable antibodies by 67 days of age, and a limited immune response could be measured at 109 days of age.
Asunto(s)
Anticuerpos Antivirales/sangre , Herpesvirus Suido 1/inmunología , Seudorrabia/inmunología , Enfermedades de los Porcinos/inmunología , Vacunas Virales/administración & dosificación , Animales , Animales Recién Nacidos , Femenino , Inmunidad Materno-Adquirida/inmunología , Seudorrabia/prevención & control , Vacunas contra la Seudorrabia , Porcinos , Enfermedades de los Porcinos/prevención & control , Factores de Tiempo , Vacunas Atenuadas/administración & dosificación , Vacunas Atenuadas/inmunología , Vacunas Virales/inmunologíaRESUMEN
The correlation between the antimicrobic standard disk agar diffusion procedure and a veterinary antimicrobic breakpoint minimal inhibitory concentration system was evaluated. Bacterial isolates representing 5 different genera were tested against 15 antimicrobics. There were 3,795 tests performed; 77.3% of the test results were in agreement, 22.6% were in disagreement. Thirty-one percent of the conflicting results were due to the organism being intermediate on the standard disk agar diffusion procedure. Results suggest that the breakpoint system needs more than 1 antimicrobic dilution per antimicrobic, or a change in some of the single dilutions in the breakpoint system would eliminate some of these discrepancies. Another possibility would be to utilize more than 1 panel design, i.e., 1 for gram-positive organisms, 1 for gram-negative organisms, and 1 for fastidious organisms.
Asunto(s)
Antibacterianos/farmacología , Bacterias/efectos de los fármacos , Pruebas de Sensibilidad Microbiana/veterinariaRESUMEN
Thirteen cases of a previously undescribed parvoviral infection affecting puppies ranging in age from 5 to 21 days is described. The cases were originally thought to represent an unusual pathologic manifestation of canine parvovirus-2 (CPV-2) infection. However, failure to confirm CPV-2 infection in any of the cases suggested a different parvovirus was involved. Minute virus of canines (MVC) was subsequently isolated from a case by using the Walter Reed Canine Cell Line, the only cell line which will support the growth of MVC. The pathologic and virologic findings for these 13 cases are described in this report.
Asunto(s)
Enfermedades de los Perros/microbiología , Infecciones por Parvoviridae/veterinaria , Parvoviridae/aislamiento & purificación , Animales , Animales Lactantes , Línea Celular , Enfermedades de los Perros/patología , Perros , Duodeno/patología , Femenino , Contenido Digestivo/microbiología , Yeyuno/microbiología , Yeyuno/patología , Yeyuno/ultraestructura , Masculino , Microscopía Electrónica , Microvellosidades/microbiología , Microvellosidades/ultraestructura , Parvoviridae/ultraestructura , Infecciones por Parvoviridae/microbiología , Infecciones por Parvoviridae/patología , Virión/aislamiento & purificación , Virión/ultraestructuraRESUMEN
Respiratory syncytial virus was the cause of a severe epizootic of bovine respiratory tract disease. The virus, isolated from a sick cow during the epizootic, produced cytopathic effect in a bovine turbinate cell line 14 days after it was inoculated. Additional support for the diagnosis came from the results of pathologic and serologic examinations. Lesions consistently present were severe necrotizing bronchiolitis and epithelial syncytia projecting from bronchiolar and alveolar walls. Also, in the cow from which the virus was isolated, there was tracheitis with a syncytial-like change involving the mucosal epithelium.
Asunto(s)
Enfermedades de los Bovinos/epidemiología , Brotes de Enfermedades/veterinaria , Infecciones por Respirovirus/veterinaria , Factores de Edad , Animales , Bronquiolitis Viral/microbiología , Bronquiolitis Viral/patología , Bronquiolitis Viral/veterinaria , Bovinos , Enfermedades de los Bovinos/microbiología , Enfermedades de los Bovinos/patología , Femenino , Georgia , Pulmón/microbiología , Pulmón/patología , Virus Sincitiales Respiratorios , Infecciones por Respirovirus/epidemiología , Infecciones por Respirovirus/microbiología , Infecciones por Respirovirus/patologíaRESUMEN
Eastern encephalomyelitis virus was isolated from the brain of 2 calves with encephalomyelitis. Using the isolant from 1 of these calves, the disease was reproduced in a clinically normal calf. Histopathologic features conformed with those described for eastern encephalomyelitis in the horse.
Asunto(s)
Enfermedades de los Bovinos , Encefalomielitis/veterinaria , Animales , Encéfalo/patología , Bovinos , Enfermedades de los Bovinos/patología , Virus de la Encefalitis Equina del Este , Encefalomielitis/patologíaRESUMEN
An epizootic of equine infectious anemia (EIA) involved 35 horses on a farm in south Georgia. During a 126-day period, 21 of these horses became seropositive for EIA. After the initial diagnosis in July, the horses were tested every 7 to 10 days. At least one additional horse was found to be seropositive on each testing day. As soon as they were determined to be seropositive, the horses were removed from the herd and sent to slaughter. The removal of the seropositive horses, however, did not stop the epizootic. We believe the initial infection was from a 7-year-old stallion that recently had been purchased or from 1 of 2 mares that were seropositive for EIA on the first test. None of the horses had been tested for EIA at the time of purchase or within 60 days before the epizootic.
Asunto(s)
Anemia Infecciosa Equina/epidemiología , Animales , Anticuerpos Antivirales/análisis , Anemia Infecciosa Equina/inmunología , Anemia Infecciosa Equina/transmisión , Femenino , Georgia , Caballos , Virus de la Anemia Infecciosa Equina/inmunología , MasculinoRESUMEN
Leptospira interrogans serovars grippotyphosa and ballum were isolated from kidney and urine of an American Foxhound pup. The pup was from a litter of 12, all of which were unthrifty. Titers for serovar grippotyphosa in pups from the litter ranged from 200 to 6,400 and 23 of 36 adult dogs in the kennel had titers to that serovar. None of the sera was tested for antibodies to serovar ballum. Leptospires were not isolated from or observed in 2 littermates and 1 penmate, but gram-positive organisms morphologically compatible with Encephalitozoon cuniculi were detected in their brains and kidneys.
Asunto(s)
Enfermedades de los Perros/microbiología , Infecciones Protozoarias en Animales , Enfermedad de Weil/veterinaria , Animales , Enfermedades de los Perros/patología , Perros , Encephalitozoon cuniculi , Leptospira interrogans/aislamiento & purificación , Infecciones por Protozoos/microbiología , Infecciones por Protozoos/patología , Enfermedad de Weil/microbiología , Enfermedad de Weil/patologíaRESUMEN
A group of 14 pregnant mares was exposed via contact to 4 mares bred to stallions infected with equine viral arteritis virus. There was a demonstrable febrile response in each donor mare and in 12 of the pregnant mares. All 18 mares became seropositive after exposure. Equine viral arteritis virus was isolated from the nasopharynx of 5 pregnant mares, but not from the donor mares. Ten of the pregnant mares aborted, and virus was isolated from fetal specimens or placenta of 8.
Asunto(s)
Aborto Veterinario/etiología , Equartevirus , Enfermedades de los Caballos/etiología , Virus ARN , Virosis/veterinaria , Animales , Equartevirus/aislamiento & purificación , Femenino , Feto/microbiología , Enfermedades de los Caballos/transmisión , Caballos , Masculino , Nasofaringe/microbiología , Embarazo , Virus ARN/aislamiento & purificación , Enfermedades de Transmisión Sexual/etiología , Enfermedades de Transmisión Sexual/veterinaria , Factores de Tiempo , Virosis/etiología , Virosis/transmisiónAsunto(s)
Enfermedades de los Perros/patología , Encefalitis de California/veterinaria , Virus La Crosse/aislamiento & purificación , Enfermedad Aguda , Animales , Encéfalo/microbiología , Encéfalo/patología , Enfermedades de los Perros/microbiología , Perros , Encefalitis de California/microbiología , Encefalitis de California/patología , NecrosisAsunto(s)
Infecciones por Adenoviridae/veterinaria , Adenovirus Caninos/aislamiento & purificación , Carnívoros/microbiología , Ursidae/microbiología , Infecciones por Adenoviridae/diagnóstico , Infecciones por Adenoviridae/patología , Adenovirus Caninos/ultraestructura , Animales , Encéfalo/microbiología , Encéfalo/patología , Cuerpos de Inclusión Viral , Hígado/microbiología , Hígado/patologíaAsunto(s)
Peste Porcina Clásica/diagnóstico , Encefalitis/veterinaria , Técnica del Anticuerpo Fluorescente , Técnicas Histológicas , Animales , Encéfalo/patología , Peste Porcina Clásica/patología , Técnicas de Cultivo , Diagnóstico Diferencial , Encefalitis/diagnóstico , Tonsila Palatina/inmunología , ARN Viral/aislamiento & purificación , Bazo/inmunología , PorcinosAsunto(s)
Infecciones por Herpesviridae/veterinaria , Enfermedades de los Caballos/etiología , Enfermedades del Sistema Nervioso/veterinaria , Animales , Anticuerpos Antivirales/análisis , Encéfalo/patología , Femenino , Infecciones por Herpesviridae/complicaciones , Infecciones por Herpesviridae/patología , Herpesvirus Équido 1/inmunología , Enfermedades de los Caballos/patología , Caballos , Masculino , Parálisis/etiología , Parálisis/patología , Parálisis/veterinariaAsunto(s)
Virus de la Encefalitis , Encefalomielitis Equina/veterinaria , Enfermedades de los Porcinos/etiología , Animales , Encéfalo/microbiología , Encéfalo/patología , Células Cultivadas , Efecto Citopatogénico Viral , Virus de la Encefalitis/patogenicidad , Encefalomielitis Equina/microbiología , Encefalomielitis Equina/patología , Riñón , Microscopía Electrónica , Porcinos , Enfermedades de los Porcinos/microbiología , Enfermedades de los Porcinos/patología , Cultivo de VirusRESUMEN
Acetone fixation and fluorescent-antibody staining of virus-infected cell cultures were performed in plastic plates. Proper addition of acetone as a fixative did not alter the plastic.
Asunto(s)
Técnica del Anticuerpo Fluorescente , Virus/aislamiento & purificación , Acetona , Línea Celular , Plásticos , Virus/crecimiento & desarrolloRESUMEN
A method for improving the original Galton microtechnique for detecting leptospiral antibodies has been developed. Simultaneous titrations were performed on 281 animal and human sera and 17 hyperimmune sera with the microscopic agglutination (MA) test and the improved microtechnique. Reproducibility of the improved microtechnique was determined independently on 65 animal sera by two laboratory sections. The results obtained by comparing positive test data from human and animal sera indicated that agreement between the original MA test and this new method exceeded 94%, whereas the original Galton microtechnique and the original MA test agreed in a maximum of 77% of the tests. This study indicates that the results obtained with the improved microtechnique are much more comparable to results obtained with the original MA test than are those obtained with the original Galton microtechnique.