Evolution of neuronal cell classes and types in the vertebrate retina.

The basic plan of the retina is conserved across vertebrates, yet species differ profoundly in their visual needs1. Retinal cell types may have evolved to accommodate these varied needs, but this has not been systematically studied. Here we generated and integrated single-cell transcriptomic atlases of the retina from 17 species: humans, two non-human primates, four rodents, three ungulates, opossum, ferret, tree shrew, a bird, a reptile, a teleost fish and a lamprey. We found high molecular conservation of the six retinal cell classes (photoreceptors, horizontal cells, bipolar cells, amacrine cells, retinal ganglion cells (RGCs) and Müller glia), with transcriptomic variation across species related to evolutionary distance. Major subclasses were also conserved, whereas variation among cell types within classes or subclasses was more pronounced. However, an integrative analysis revealed that numerous cell types are shared across species, based on conserved gene expression programmes that are likely to trace back to an early ancestral vertebrate. The degree of variation among cell types increased from the outer retina (photoreceptors) to the inner retina (RGCs), suggesting that evolution acts preferentially to shape the retinal output. Finally, we identified rodent orthologues of midget RGCs, which comprise more than 80% of RGCs in the human retina, subserve high-acuity vision, and were previously believed to be restricted to primates2. By contrast, the mouse orthologues have large receptive fields and comprise around 2% of mouse RGCs. Projections of both primate and mouse orthologous types are overrepresented in the thalamus, which supplies the primary visual cortex. We suggest that midget RGCs are not primate innovations, but are descendants of evolutionarily ancient types that decreased in size and increased in number as primates evolved, thereby facilitating high visual acuity and increased cortical processing of visual information.

Genome-wide detection of selection signatures in Jianli pigs reveals novel cis-regulatory haplotype in EDNRB associated with two-end black coat color.

BACKGROUND: Jianli pig, a renowned indigenous breed in China, has the characteristics of a two-end black (TEB) coat color, excellent meat quality, strong adaptability and increased prolificacy. However, there is limited information available regarding the genetic diversity, population structure and genomic regions under selection of Jianli pig. On the other hand, the genetic mechanism of TEB coat color has remained largely unknown. RESULTS: In this study, the whole genome resequencing of 30 Jianli pigs within a context of 153 individuals representing 13 diverse breeds was performed. The population structure analysis revealed that Jianli pigs have close genetic relationships with the Tongcheng pig breed, their geographical neighbors. Three methods (observed heterozygosity, expected heterozygosity, and runs of homozygosity) implied a relatively high level of genetic diversity and, a low inbreeding coefficient in Jianli compared with other pigs. We used Fst and XP-EHH to detect the selection signatures in Jianli pigs compared with Asian wild boar. A total of 451 candidate genes influencing meat quality (CREBBP, ADCY9, EEPD1 and HDAC9), reproduction (ESR1 and FANCA), and coat color (EDNRB, MITF and MC1R), were detected by gene annotation analysis. Finally, to fine-map the genomic region for the two-end black (TEB) coat color phenotype in Jianli pigs, we performed three signature selection methods between the TEB coat color and no-TEB coat color pig breeds. The current study, further confirmed that the EDNRB gene is a candidate gene for TEB color phenotype found in Chinese pigs, including Jinhua pigs, and the haplotype harboring 25 SNPs in the EDNRB gene may promote the formation of TEB coat color. Further ATAC-seq and luciferase reporter assays of these regions suggest that the 25-SNPs region was a strong candidate causative mutation that regulates the TEB coat color phenotype by altering enhancer function. CONCLUSION: Our results advanced the understanding of the genetic mechanism behind artificial selection, and provided further resources for the protection and breeding improvement of Jianli pigs.

Limits of mitochondrial genes in delimiting species within a Carbula species complex (Hemiptera: Pentatomidae).

Molecular data has become a powerful tool for species delimitation, particularly among those that present limited morphological differences; while the mitochondrial genome, with its moderate length, low cost of sequencing and fast lineage sorting, has emerged as a practical data set. Due to the limited morphological differences among the closely related species of Carbula Stål 1865, the species boundaries between Carbula abbreviata (Motschulsky, 1866), Carbula humerigera (Uhler, 1860), and Carbula putoni (Jakovlev, 1876) have remained particularly unclear. In this study, we applied two phylogenetic reconstruction methods to two data sets (mitogenome and COI) to assess the phylogeny of Carbula distributed in Asia, and five species delimitation methods to determine the boundaries between East Asian Carbula species. Our phylogenetic analyses showed Carbula to be paraphyletic; the seven known species distributed within East Asia to form a single monophyletic group, and within this, C. abbreviata, C. humerigera, C. putoni and middle-type to comprise a C. humerigera species complex. Our results show that mitogenome data alone, while effective in the differentiation of more distantly related Carbula species, is not sufficient to accurately delimit the species within this newly described complex.

Coronal deformity in ankylosing spondylitis with concomitant thoracolumbar kyphosis: patterns, manifestations and surgical strategies.

PURPOSE: To evaluate different patterns of coronal deformity secondary to ankylosing spondylitis (AS), to propose relevant treatment strategies, and to assess efficacy of asymmetrical pedicle subtraction osteotomy (APSO). METHODS: Coronal deformity was defined as coronal Cobb angle over 20º or coronal balance distance (CBD) more than 3 cm. 65 consecutive AS patients with concomitant coronal and sagittal deformity who underwent PSO were included. The average follow-up time was 40.4 months. Radiographic evaluation included coronal Cobb angle and CBD. Furthermore, sagittal parameters were used to assess magnitude and maintenance of kyphosis correction. RESULTS: Based on curve characteristics, coronal deformity caused by AS included four different radiologic patterns: Pattern I: lumbar scoliosis; Pattern II: C-shaped thoracolumbar curve; Pattern III: trunk shift without major curve; Pattern IV: proximal thoracic scoliosis. APSO was performed for patients in Pattern I to III while conventional PSO was applied for patients in Pattern IV. Significant improvement in all the sagittal parameters were noted in 65 patients without obvious correction loss at the last follow-up. Besides, significant and sustained correction of coronal mal-alignment was identified in 59 APSO-treated patients. Rod fracture occurred in four cases and revision surgery was performed for one case. CONCLUSION: According to radiologic manifestations, coronal deformity caused by AS could be categorized into four patterns. APSO proved to be a feasible and effective procedure for correction of Pattern I to III patients. Coronal deformity pattern, apex location, sagittal profile of lumbar spine and preoperative hip function should be considered for osteotomy level selection in APSO.

Countermovement Jump and Momentum Generation Associations to Fastball Velocity Performance Among Division I Collegiate Pitchers.

ABSTRACT: Sakurai, M, Qiao, M, Szymanski, DJ, and Crotin, RL. Countermovement jump and momentum generation associations to fastball velocity performance among Division I collegiate pitchers. J Strength Cond Res 38(7): 1288-1294, 2024-The current study explored the relationships between countermovement jump (CMJ) profiles and baseball pitching performance. Nineteen Division I collegiate pitchers performed in-laboratory pitching and bilateral CMJs. Whole-body kinematics and ground reaction force were collected during both pitching and CMJ evaluations. Statistically significant correlations of concentric impulse and peak power in the CMJ test with fastball velocity were observed (r = 0.71 and 0.68). Concentric impulse in CMJ also showed a statistically significant correlation with linear momentum in the anterior-posterior direction during pitching (r = 0.68). Lean body mass and body mass showed statistically significant correlations with both of the 2 linear momentums during pitching (r = 0.71∼0.83), and concentric impulse in CMJ (r = 0.71 and 0.81). Pelvis and trunk pitching mechanics did not correlate with any of the CMJ variables at the statistically significant level, whereas the direction of the correlations varied (|r| < 0.45). Assessment of a baseball pitcher's CMJ should focus on concentric impulse and peak power because only these showed meaningful relationships with fastball velocity or momentum generation during pitching. An increase in lean body mass is also suggested to be able to generate more impulse and momentum. Baseball coaches, strength coaches, and clinicians are encouraged to include lower-body explosive training to enhance the force and power output capacity of baseball pitchers.

Coded aperture compressive temporal imaging using complementary codes and untrained neural networks for high-quality reconstruction.

The coded aperture compressive temporal imaging (CACTI) modality is capable of capturing dynamic scenes with only a single-shot of a 2D detector. In this Letter, we present a specifically designed CACTI system to boost the reconstruction quality. Our design is twofold: for the optical encoder, we use complementary codes instead of random ones as widely adopted before; for the reconstruction algorithm, an untrained neural network-based algorithm is developed. Experimental and simulation tests show that such co-design of encoding-decoding produces superior image quality over other CACTI schemes using random codes and other optimization algorithms. In addition, a dual-prism design in the optical system improves the light efficiency by approximately a factor of four compared with previous systems.

Full-resolution and full-dynamic-range coded aperture compressive temporal imaging.

Coded aperture compressive temporal imaging (CACTI) aims to capture a sequence of video frames in a single shot, using an off-the-shelf 2D sensor. This approach effectively increases the frame rate of the sensor while reducing data throughput requirements. However, previous CACTI systems have encountered challenges such as limited spatial resolution and a narrow dynamic range, primarily resulting from suboptimal optical modulation and sampling schemes. In this Letter, we present a highly efficient CACTI system that addresses these challenges by employing precise one-to-one pixel mapping between the sensor and modulator, while using structural gray scale masks instead of binary masks. Moreover, we develop a hybrid convolutional-Transformer deep network for accurate reconstruction of the captured frames. Both simulated and real data experiments demonstrate the superiority of our proposed system over previous approaches, exhibiting significant improvements in terms of spatial resolution and dynamic range.

Combined Countermovement Jump Testing and Motion Analysis as the Future of Performance Assessment for Baseball Pitchers: A Narrative Review.

ABSTRACT: Sakurai, M, Szymanski, DJ, Qiao, M, and Crotin, RL. Combined countermovement jump testing and motion analysis as the future of performance assessment for baseball pitchers: a narrative review. J Strength Cond Res 37(6): 1327-1338, 2023-Whole-body kinematics and kinetics impact pitching performance, and when coordination of momentum transfer is impacted, throwing-related injury risks increase. Although correlations between overhead throwing velocity and lower-body power measured by jump tests have been reported by previous studies, further research is needed to identify these relationships to better understand pitching mechanics and the validity and application of jump tests for the assessment of baseball pitchers. This review communicates effective whole-body pitching mechanics, including throwing arm, trunk, and pelvis, and lower body, as well as discusses the applicability of strength and power field tests to deepen training insights in establishing more efficient motor patterns. If both lower-body power and coordination of momentum transfer are optimized, baseball pitchers may experience reduced throwing arm stress. The overarching insight to be gained from this review article is that high performance in baseball pitching is multifactorial, and attention to both strength and skill coaching is paramount. As a result, the intersection of lower-body power testing and biomechanical analysis can play an important role in uniting strength and conditioning professionals, clinicians, pitching coaches, and biomechanics experts to advance pitching performance and health in the sport of baseball.

Exploratory Investigation Into the Impact of Bilateral and Unilateral Jump Characteristics on Ground Reaction Force Applications in Baseball Pitching.

ABSTRACT: Lis, R, Szymanski, DJ, Qiao, M, and Crotin, RL. An exploratory investigation into the impact of bilateral and unilateral jump characteristics on ground reaction force applications in baseball pitching. J Strength Cond Res 37(9): 1852-1859, 2023-Jump tests are effective, valid, and reliable in examining lower-body power that can influence ground reaction forces (GRFs) in baseball pitching. Relationships between drive and stride leg GRFs and fastball velocities while pitching from the wind-up and stretch were evaluated for performance influences across the following conditions: (a) lower-body GRFs from unilateral countermovement jumps (UCMJ) and bilateral countermovement jumps (BCMJ) and (b) BCMJ and drive and stride leg UCMJ height. Nineteen Division I collegiate baseball pitchers (age; 19.9 ± 1.5 years, height; 1.86 ± 0.06 m, body mass; 90.7 ± 13.8 kg) completed BCMJ and UCMJ tests and threw four-seam fastballs from a pitching mound with 2 embedded force plates. Three moderate associations ( r ≥ 0.47) were statistically significant ( p < 0.05) for BCMJ and UCMJ heights to pitching GRFs. Stride leg UCMJ height was significantly greater than drive leg UCMJ height, p < 0.01, η 2 = 0.34. Wind-up and stretch GRFs were statistically similar. Fastball velocities showed a statistically significant association that was moderately high with wind-up and stretch stride leg anterior-posterior GRFs ( r ≥ 0.65, p < 0.01). Collegiate pitchers jumped significantly higher with their stride leg, and the sum of vertical unilateral jump height of both legs was significantly greater (27%) than BCMJ height to indicate greater single-leg dominance in jumping. Although stride leg height was higher, improving stride leg jumping performance may be functionally more important in producing greater momentum into foot strike that has the potential to increase fastball velocity.

Multilayer Perceptron Network Optimization for Chaotic Time Series Modeling.

Chaotic time series are widely present in practice, but due to their characteristics-such as internal randomness, nonlinearity, and long-term unpredictability-it is difficult to achieve high-precision intermediate or long-term predictions. Multi-layer perceptron (MLP) networks are an effective tool for chaotic time series modeling. Focusing on chaotic time series modeling, this paper presents a generalized degree of freedom approximation method of MLP. We then obtain its Akachi information criterion, which is designed as the loss function for training, hence developing an overall framework for chaotic time series analysis, including phase space reconstruction, model training, and model selection. To verify the effectiveness of the proposed method, it is applied to two artificial chaotic time series and two real-world chaotic time series. The numerical results show that the proposed optimized method is effective to obtain the best model from a group of candidates. Moreover, the optimized models perform very well in multi-step prediction tasks.

Toxicity of Chlorantraniliprole and it's Formulated Product, Altacor®, to Individuals and Populations of Ceriodaphnia Dubia Richard.

The toxicity of the insecticide chlorantraniliprole and its formulated product Altacor® was determined for the Cladoceran, Ceriodaphnia dubia Richard. Acute toxicity (48 h) and 21 d population studies were conducted. The hypothesis of this study was that these two compounds would have different toxicities. We conducted acute and chronic toxicity studies for each compound and compared the results to test this hypothesis. 48 h LC50s (95% CL) for chlorantraniliprole and Altacor® were 8.5 (6.6-11.5) and 6.0 (3.7-9.0) µg chlorantraniliprole/L water, respectively. Therefore, chlorantraniliprole and Altacor® were equitoxic to C. dubia at LC50 based on overlap of the 95% CL. In the population study, chlorantraniliprole and Altacor® concentrations equivalent to the acute LC5, 10, 25, and 50 for each product were evaluated on populations of C. dubia. Number of individuals after 21 d was the endpoint evaluated. T-tests conducted at each LC value indicated that there was no significant difference in population size between these two products at each LC value evaluated. Previous studies show that toxicity can vary greatly between formulated and technical grade pesticides. However, our results show that chlorantraniliprole and its formulated product, Altacor® were equally toxic to C. dubia. Therefore, making assumptions about the toxicity of formulated and unformulated products is ill advised.

Current status of unplanned readmission of neonates within 31 days after discharge from the neonatal intensive care unit and risk factors for readmission. / 新生儿重症监护室患儿出院31 då† éžè®¡åˆ’å†å ¥é™¢çŽ°çŠ¶åŠå½±å“å› ç´ åˆ†æž.

OBJECTIVES: To investigate the current status of unplanned readmission of neonates within 31 days after discharge from the neonatal intensive care unit (NICU) and risk factors for readmission. METHODS: A retrospective analysis was performed on the medical data of 1 561 infants discharged from the NICU, among whom 52 infants who were readmitted within 31 days were enrolled as the case group, and 104 infants who were not readmitted after discharge during the same period of time were enrolled as the control group. Univariate analysis and multivariate logistic regression analysis were performed to identify the risk factors for readmission. RESULTS: Among the 1 561 infants, a total of 63 readmissions occurred in 52 infants, with a readmission rate of 3.33%. hyperbilirubinemia and pneumonia were the main causes for readmission, accounting for 29% (18/63) and 24% (15/63) respectively. The multivariate logistic regression analysis showed that that gestational age <28 weeks, birth weight <1 500 g, multiple pregnancy, mechanical ventilation, and length of hospital stay <7 days were risk factors for readmission (OR=5.645, 5.750, 3.044, 3.331, and 1.718 respectively, P<0.05). CONCLUSIONS: Neonates have a relatively high risk of readmission after discharge from the NICU. The medical staff should pay attention to risk factors for readmission and formulate targeted intervention measures, so as to reduce readmission and improve the quality of medical service.

Wired Microfabricated Electrochemical Systems.

Metal wires have been used as an alternative to liquid junctions for the connection of solutions in microfabricated electrochemical devices. They exhibit similar performance to liquid junctions, provided that the interfacial potentials at both ends of the wires were appropriately canceled. Cyclic voltammograms of devices with liquid junctions and metal wires were very similar when no current or a low current flowed through the metal wire between the working and reference electrodes. Iridium wires with iridium oxide at both ends facilitated canceling of the interfacial potentials at either end of the junction particularly well, and were used effectively for voltammetry, amperometry, and potentiometry by adjusting the pH of the solutions in the working and reference electrode compartments to be equal. This approach was used to effectively integrate a reliable common reference electrode between multiple working electrodes and to conduct automated electrochemical control of solution transport in microfluidic systems.

Snapshot temporal compressive microscopy using an iterative algorithm with untrained neural networks.

We report a snapshot temporal compressive microscopy imaging system, using the idea of video compressive sensing, to capture high-speed microscopic scenes with a low-speed camera. An untrained deep neural network is used in our iterative inversion algorithm to reconstruct 20 high-speed video frames from a single compressed measurement. Specifically, using a camera working at 50 frames per second (fps) to capture the measurement, we can recover videos at 1000 fps. Our deep neural network is embedded in the inversion algorithm, and its parameters are learned simultaneously with the reconstruction.

Double-Electromagnetically-Induced-Transparency Ground-State Cooling of Stationary Two-Dimensional Ion Crystals.

We theoretically and experimentally investigate double-electromagnetically-induced transparency (double-EIT) cooling of two-dimensional ion crystals confined in a Paul trap. The double-EIT ground-state cooling is observed for ^{171}Yb^{+} ions with a clock state, for which EIT cooling has not been realized like many other ions with a simple Λ scheme. A cooling rate of n[over ¯][over ˙]=34(±1.8) ms^{-1} and a cooling limit of n[over ¯]=0.06(±0.059) are observed for a single ion. The measured cooling rate and limit are consistent with theoretical predictions. We apply double-EIT cooling to the transverse modes of two-dimensional (2D) crystals with up to 12 ions. In our 2D crystals, the micromotion and the transverse mode directions are perpendicular, which makes them decoupled. Therefore, the cooling on transverse modes is not disturbed by micromotion, which is confirmed in our experiment. For the center of mass mode of a 12-ion crystal, we observe a cooling rate and a cooling limit that are consistent with those of a single ion, including heating rates proportional to the number of ions. This method can be extended to other hyperfine qubits, and near ground-state cooling of stationary 2D crystals with large numbers of ions may advance the field of quantum information sciences.

Failure patterns and related risk factors of sagittal reconstruction following pedicle subtraction osteotomy in patients with ankylosing spondylitis and thoracolumbar kyphosis.

OBJECTIVE: The aim of this study was to analyze the specific patterns and risk factors of sagittal reconstruction failure in ankylosing spondylitis (AS)-related thoracolumbar kyphosis after pedicle subtraction osteotomy (PSO). METHODS: A retrospective study was performed in patients with AS and thoracolumbar kyphosis after lumbar PSO with a minimum follow-up of 2 years. Patients were classified as having successful realignment (group A), inadequate correction immediately postoperatively (group B), and sagittal decompensation during follow-up (group C) according to the immediately postoperative and latest follow-up sagittal vertical axis (SVA). Radiographic parameters and clinical outcomes were collected. Pelvic tilt (PT) was used to assess the magnitude of pelvic backward rotation. Hip structural damage and ossification of the anterior longitudinal ligament (ALL) at the proximal junction, PSO level, and distal junction were also evaluated on radiographs. RESULTS: Overall, 109 patients with a mean age of 35.3 years were included. Patients in both group B (n = 16) and group C (n = 13) were older than those in group A (n = 80) (mean ages 43.6 vs 32.9 years, p < 0.011; and 39.2 vs 32.9 years, p = 0.018; respectively). Age (OR 1.102, p = 0.011), and preoperative PT (OR 1.171, p = 0.041) and SVA (OR 1.041, p = 0.016) were identified as independent risk factors of inadequate correction. Additionally, a higher distribution of patients with adequate ALL ossification at the PSO level was found in group B than in group A (37.5% vs 22.5%, p = 0.003). Age (OR 1.101, p = 0.011) and preoperative SVA (OR 1.013, p = 0.020) were identified as independent risk factors of sagittal decompensation. Furthermore, compared with group A, group C showed a higher distribution of patients with severe hip structural damage (15.4% vs 0, p = 0.018) and higher incidences of rod fracture (RF) (38.5% vs 8.8%, p = 0.011) and pseudarthrosis (15.4% vs 0, p = 0.018). Additionally, the incidence of RF (19.6% vs 6.9%, p = 0.045) and changes in the proximal junctional angle (0.5° vs 2.2°, p = 0.027) and the distal junctional angle (0.3° vs 2.2°, p = 0.019) were lower during follow-up in patients with adequate ALL ossification than in those without adequate ossification. CONCLUSIONS: Sagittal reconstruction failure in patients with AS could be attributed to inadequate correction immediately after surgery (14.7%) and sagittal decompensation during follow-up (11.9%). Adequate ALL ossification was a risk factor of inadequate correction. However, adequate ALL ossification could decrease the development of RF and relieve the junctional kyphotic change during follow-up. Older age and greater baseline SVA were independent risk factors for both inadequate correction and sagittal decompensation.

Genome-wide analysis of long noncoding RNA and mRNA profiles in PRRSV-infected porcine alveolar macrophages.

Porcine reproductive and respiratory syndrome (PRRS), which is caused by PRRS virus (PRRSV), is one of the most globally devastating swine diseases. It is essential to develop new strategy to control PRRS via an understanding of mechanisms that PRRSV utilizes to interfere with the host's innate immunity. In this study, we deeply sequenced and analyzed long noncoding RNA (lncRNA) and mRNA expression profiles of the porcine alveolar macrophages (PAMs) after PRRSV infection. 126 lncRNAs and 753 mRNAs were differentially expressed between PRRSV-infected and control PAMs. The co-expressed genes of down-regulated lncRNAs were significantly enriched within NF-kappa B and toll-like receptor signaling pathways. Co-expression network analysis indicated that part of the dysregulated lncRNAs associated with the interferon-induced genes. These dysregulated lncRNAs may play an important role in the host's innate immune responses to PRRSV infection. However, further research is required to characterize the function of these lncRNAs.

Leg Joint Mechanics When Hopping at Different Frequencies.

Although the dynamics of center of mass can be accounted for by a spring-mass model during hopping, less is known about how each leg joint (ie, hip, knee, and ankle) contributes to center of mass dynamics. This work investigated the function of individual leg joints when hopping unilaterally and vertically at 4 frequencies (ie, 1.6, 2.0, 2.4, and 2.8 Hz). The hypotheses are (1) all leg joints maintain the function as torsional springs and increase their stiffness when hopping faster and (2) leg joints are controlled to maintain the mechanical load in the joints or vertical peak accelerations at different body locations when hopping at different frequencies. Results showed that all leg joints behaved as torsional springs during low-frequency hopping (ie, 1.6 Hz). As hopping frequency increased, leg joints changed their functions differently; that is, the hip and knee shifted to strut, and the ankle remained as spring. When hopping fast, the body's total mechanical energy decreased, and the ankle increased the amount of energy storage and return from 50% to 62%. Leg joints did not maintain a constant load at the joints or vertical peak accelerations at different body locations when hopping at different frequencies.

Realistic phase screen model for forward multiple-scattering media.

Existing random phase screen (RPS) models for forward multiple-scattering media fail to incorporate ballistic light. In this Letter, we redesign the angular spectrum of the screen by means of Monte Carlo simulation based on an assumption that a single screen should represent all the scattering events a photon experiences between two adjacent screens. Three examples demonstrate that the proposed model exhibits more realistic optical properties than conventional RPS models in terms of attenuation of ballistic light, evolution of beam profile, and angular memory effect. The proposed model also provides the flexibility to balance the computing accuracy, speed, and memory usage by tuning the screen spacing.

Snapshot spatial-temporal compressive imaging.

We build a compressive imaging system that can reconstruct high-speed videos of two fields-of-view by capturing a single frame. A single digital micromirror device is employed to implement high-speed modulation simultaneously on both scenes in a manner that does not compromise spatial resolution. We utilize deep denoising priors in the plug-and-play framework for efficient reconstruction. Two spatial-temporal data cubes, each with the size $ 650 \times 650 \times 20 $650×650×20, are reconstructed from a single two-dimensional spatial measurement.