Synaptic endocytosis in adult adipose stromal cell-derived neurons.

Synapses are essential for facilitating the transmission of information between neurons and for executing neurophysiological processes. Following the exocytosis of neurotransmitters, the synaptic vesicle may quickly undergo endocytosis to preserve the structural integrity of the synapse. When converting adipose-derived stromal cells (ADSCs) into neurons, the ADSCs have already demonstrated comparable morphology, structure, and electrophysiological characteristics to neurons. Nevertheless, there is currently no published study on the endocytotic function of neurons that are produced from ADSCs. This study aimed to examine synaptic endocytosis in neurons derived from ADSCs by qualitatively and quantitatively analyzing the presence of Ap-2, Clathrin, Endophilin, Dynamin, and Hsc70, which are the key proteins involved in clathrin-mediated endocytosis (CME), as well as by using FM1-43 and cadmium selenide quantum dots (CdSe QDs). Additionally, single-cell RNA sequencing (scRNA-seq) was used to look at the levels of both neuronal markers and markers related to CME at the same time. The results of this study provide evidence that synapses in neurons produced from ADSCs have a role in endocytosis, mainly through the CME route.

Influence of Welding Degree on the Meso-Mechanical Anisotropy, Fracture Propagation, and Fracture Surface Roughness of Welded Tuff.

Welded tuffs have a wide range of welding degrees and show significant variability in mechanical behavior. However, the detailed influence of welding degree on the meso-mechanical behavior of welded tuffs remains unclear. Based on petrographic and pore-structure analysis, we conducted a series of meso-mechanical experiments on weakly to strongly welded tuffs by utilizing a mesoscale real-time loading-observation-acquisition system. The results indicated that the strongly and weakly welded tuffs showed a small range in mineralogical composition and porosity, while the meso-mechanical behavior exhibited significant variability. Strongly welded tuffs showed lower uniaxial compression strength, weaker mechanical anisotropy, and smaller fracture surface roughness. In contrast, weakly welded tuffs exhibited higher uniaxial compression strength, stronger mechanical anisotropy, and rougher fracture surface roughness. Welded tuffs with strong packing and welding of glass shards tended to have fractures propagating along the maximum principal direction, while those with weak packing and welding of glass shards may have had failure along the alignment of glass shards. The influence of welding degree on the meso-mechanical behavior of welded tuffs probably originates from their diagenesis environments, mainly depending on the combined effect of the pyroclastic properties and pseudo-rhyolitic structure. The findings reveal the meso-mechanical differences of welded tuffs and shed light on improving tuffs for stable and durable construction.

Influence of Natural Fractures and Laminae on Fracture Propagation and Failure Mode of Continental Shale.

Natural fractures and laminae are well-developed in continental shale, which greatly affects the fracture propagation and failure mode. Based on the natural fractures and laminae developed in the outcrops of Triassic continental shale from the southern Ordos Basin, China, four different types of shale models are constructed in this research. The CASRock software V1.0 is utilized to conduct numerical simulations to investigate the influence of natural fractures and soft-to-hard laminae on the mechanical behavior of continental shale. The results demonstrate that the uniaxial compressive strength of shale models can improve by up to 34.48% when soft-to-hard laminae are present, but it can drop by up to 18.97% when weak interfaces are present. New fractures are consistently initiated at the ends of natural fractures, with various propagation patterns in different laminae. Fractures in soft laminae usually propagate in an oblique path at an angle ß ≈ 20°-30° relative to the direction of compressive stress, manifesting as shear fractures. Fractures in medium-to-hard laminae tend to propagate parallel to compressive stress, primarily featuring tensile fractures. The ultimate fracture morphology becomes more complex as soft, medium, and hard laminae and weak interfaces occur successively. It changes from a nearly linear fracture to an echelon pattern with more secondary fractures and finally a network shape, with a total fracture area increase of up to 270.12%. This study reveals the combined effect of natural fractures, soft-to-hard laminae, and weak interfaces on the fracture propagation and failure model of continental shale, providing support for fracturing optimization based on shale's authentic structure characteristics.

[Review on wetland water level monitoring using interferometric synthetic aperture radar]. / å¹²æ¶‰åˆæˆå­”å¾„é›·è¾¾ç›‘æµ‹æ¹¿åœ°æ°´ä½ç ”ç©¶ç»¼è¿°.

Water level is an important indicator of wetland hydrological regime. Detection of wetland water levels through interferometric synthetic aperture radar (InSAR) has outstanding advantage, including high spatial resolution, high accuracy, low cost, and high efficiency. We introduced prerequisites for the monitoring of wetland water levels with InSAR, discussed the types of InSAR techniques, the influencing factors for monitoring wetland water levels and their advantages and disadvantages. There are three prerequisites for effectively detecting wetland water levels with InSAR techniques: 1) the presence of emergent aquatic plants; 2) the main backscattering mechanism is double bounce scattering; and 3) the interferometric coherence exceeds a certain threshold. Current water level monitoring techniques have been developed from traditional InSAR techniques to advanced InSAR techniques, such as STBAS, MM, and DSI. These techniques evolve from detecting relative water level changes to estimate absolute water level and water depth time series. The influencing factors of InSAR techniques for monitoring wetland water levels include operating para-meters of the synthetic aperture radar (SAR) and characteristics of the wetlands themselves. Finally, we proposed the key directions for future research in this field: i) investigating the potential use of specific water level monitoring techniques in other regions with different backscattering and interferometric coherence characteristics; ii) developing new algorithms to integrate multi-sensor, multi-track, multi-band, multi-polarization, and multi-temporal InSAR repeat-pass observation; iii) considering alternative sources of SAR data; and (iv) strengthening research on "by-products" of wetland water level monitoring with InSAR, such as wetland hydrological connectivity, flow direction, and flow regime.

[Quantitative assessment of surface hydrological connectivity in Momoge National Nature Reserve, Northeast China]. / èŽ«èŽ«æ ¼å›½å®¶çº§è‡ªç„¶ä¿æŠ¤åŒºåœ°è¡¨æ°´æ–‡è¿žé€šæ€§å®šé‡è¯„ä¼°.

Quantitative assessment of hydrological connectivity is a hot but difficult issue in current research. Using the 30-m resolution global monthly surface water remote sensing dataset released by the EU Joint Research Center and three indicators of geostatistical connectivity, maximum distance of connection (MDC), and surface water extent of connectome (i.e., seasonally connected water bodies), we quantified the hydrological connectivity of surface water in Momoge National Nature Reserve in different months of a normal year (May to October 2016), and in September of different hydrological years (a wet year, namely 1998; a normal year, namely 2016; a drought year, namely 2002), and different directions (west-east and north-south). Our results showed that: 1) the geostatistical connectivity function (GCF) along the west-east direction was better than that along the north-south direction. The GCF in August and July was better than that in other months. The GCF along the west-east direction of each hydrological year was better than that in the drought year, whereas the GCF in the drought year was better than the corresponding value in the normal year. The GCF along the north-south direction in each hydrological year was better than that in the normal year, whereas the GCF in the normal year was better than that in the drought year. The MDC along the west-east direction in June, July, September, and October of the normal year was all concentrated at 25.26 km. MDC was more concentrated along the north-south direction, with 10 km for all months. The MDC in the normal and drought years was relatively close, but both were much smaller than that in the wet year. 2) frequent seasonal connections between the Yuelianghu Reservoir and the Nenjiang River, and between Etoupao and its neighboring lakes occurred in the study area during the normal year, while most of the other lakes remained isolated. The patterns of hydrological connection in the study area differed across different hydrological years: two giant connectomes were formed in the wet year, some lakes are periodically connected in the normal year, and all lakes remain isolated in the drought year. 3) As a drainage area for farmland receding water, the surface water extent of the Etoupao connectome increased visibly during the three water supplement seasons (spring, summer, and autumn). By quantifying the surface hydrological connectivity in Momoge National Nature Reserve with multiple water sources from different perspectives, our results provide a scientific basis for wetland protection and restoration and integrated management of watershed water resources.

Investigating the potential use of Sentinel-1 data for monitoring wetland water level changes in China's Momoge National Nature Reserve.

BACKGROUND: Interferometric Synthetic Aperture Radar (InSAR) has become a promising technique for monitoring wetland water levels. However, its capability in monitoring wetland water level changes with Sentine-1 data has not yet been thoroughly investigated. METHODS: In this study, we produced a multitemporal Sentinel-1 C-band VV-polarized SAR backscatter images and generated a total of 28 interferometric coherence maps for marsh wetlands of China's Momoge National Nature Reserve to investigate the interferometric coherence level of Sentinel-1 C-VV data as a function of perpendicular and temporal baseline, water depth, and SAR backscattering intensity. We also selected six interferogram pairs acquired within 24 days for quantitative analysis of the accuracy of water level changes monitored by Sentinel-1 InSAR. The accuracy of water level changes determined through the Sentinel-1 InSAR technique was calibrated by the values of six field water level loggers. RESULTS: Our study showed that (1) the coherence was mainly dependent on the temporal baseline and was little affected by the perpendicular baseline for Sentinel-1 C-VV data in marsh wetlands; (2) in the early stage of a growing season, a clear negative correlation was found between Sentinel-1 coherence and water depth; (3) there was an almost linear negative correlation between Sentinel-1 C-VV coherence and backscatter for the marsh wetlands; (4) once the coherence exceeds a threshold of 0.3, the stage during the growing season, rather than the coherence, appeared to be the primary factor determining the quality of the interferogram for the marsh wetlands, even though the quality of the interferogram largely depends on the coherence; (5) the results of water level changes from InSAR processing show no agreement with in-situ measurements during most growth stages. Based on the findings, we can conclude that although the interferometric coherence of the Sentinel-1 C-VV data is high enough, the data is generally unsuitable for monitoring water level changes in marsh wetlands of China's Momoge National Nature Reserve.