RESUMEN
Sufficient blood flow to tissues relies on arterial blood vessels, but the mechanisms regulating their development are poorly understood. Many arteries, including coronary arteries of the heart, form through remodeling of an immature vascular plexus in a process triggered and shaped by blood flow. However, little is known about how cues from fluid shear stress are translated into responses that pattern artery development. Here, we show that mice lacking endothelial Dach1 had small coronary arteries, decreased endothelial cell polarization, and reduced expression of the chemokine Cxcl12 Under shear stress in culture, Dach1 overexpression stimulated endothelial cell polarization and migration against flow, which was reversed upon CXCL12/CXCR4 inhibition. In vivo, DACH1 was expressed during early arteriogenesis but was down in mature arteries. Mature artery-type shear stress (high, uniform laminar) specifically down-regulated DACH1, while the remodeling artery-type flow (low, variable) maintained DACH1 expression. Together, our data support a model in which DACH1 stimulates coronary artery growth by activating Cxcl12 expression and endothelial cell migration against blood flow into developing arteries. This activity is suppressed once arteries reach a mature morphology and acquire high, laminar flow that down-regulates DACH1. Thus, we identified a mechanism by which blood flow quality balances artery growth and maturation.
Asunto(s)
Vasos Coronarios/crecimiento & desarrollo , Proteínas del Ojo/genética , Proteínas del Ojo/metabolismo , Neovascularización Fisiológica/genética , Transducción de Señal/genética , Animales , Velocidad del Flujo Sanguíneo/fisiología , Movimiento Celular/genética , Células Cultivadas , Quimiocina CXCL12/genética , Vasos Coronarios/fisiopatología , Células Endoteliales/citología , Regulación del Desarrollo de la Expresión Génica/genética , Humanos , Ratones , Ratones Endogámicos C57BL , Mutación , Técnicas de Cultivo de Órganos , Receptores CXCR4/genética , Estrés MecánicoRESUMEN
Arteries and veins are specified by antagonistic transcriptional programs. However, during development and regeneration, new arteries can arise from pre-existing veins through a poorly understood process of cell fate conversion. Here, using single-cell RNA sequencing and mouse genetics, we show that vein cells of the developing heart undergo an early cell fate switch to create a pre-artery population that subsequently builds coronary arteries. Vein cells underwent a gradual and simultaneous switch from venous to arterial fate before a subset of cells crossed a transcriptional threshold into the pre-artery state. Before the onset of coronary blood flow, pre-artery cells appeared in the immature vessel plexus, expressed mature artery markers, and decreased cell cycling. The vein-specifying transcription factor COUP-TF2 (also known as NR2F2) prevented plexus cells from overcoming the pre-artery threshold by inducing cell cycle genes. Thus, vein-derived coronary arteries are built by pre-artery cells that can differentiate independently of blood flow upon the release of inhibition mediated by COUP-TF2 and cell cycle factors.
Asunto(s)
Arterias/citología , Vasos Coronarios/citología , Análisis de la Célula Individual , Células Madre/citología , Células Madre/metabolismo , Venas/citología , Animales , Arterias/metabolismo , Factor de Transcripción COUP II/metabolismo , Ciclo Celular/genética , Diferenciación Celular , Linaje de la Célula , Vasos Coronarios/metabolismo , Femenino , Masculino , Ratones , Análisis de Secuencia de ARN , Venas/metabolismoRESUMEN
[Figure: see text].
Asunto(s)
Enfermedad de la Arteria Coronaria/metabolismo , Vasos Coronarios/metabolismo , Proteínas del Ojo/metabolismo , Animales , Diferenciación Celular , Enfermedad de la Arteria Coronaria/genética , Enfermedad de la Arteria Coronaria/patología , Vasos Coronarios/embriología , Endotelio Vascular/embriología , Endotelio Vascular/metabolismo , Proteínas del Ojo/genética , Mutación con Ganancia de Función , Metabolismo de los Lípidos , Ratones , Ratones Endogámicos C57BL , Miocardio/metabolismo , Neovascularización FisiológicaRESUMEN
Non-centrosomal microtubule organizing centers (ncMTOCs) are found in most differentiated cells, but how these structures regulate microtubule organization and dynamics is largely unknown. We optimized a tissue-specific degradation system to test the role of the essential centrosomal microtubule nucleators γ-tubulin ring complex (γ-TuRC) and AIR-1/Aurora A at the apical ncMTOC, where they both localize in Caenorhabditis elegans embryonic intestinal epithelial cells. As at the centrosome, the core γ-TuRC component GIP-1/GCP3 is required to recruit other γ-TuRC components to the apical ncMTOC, including MZT-1/MZT1, characterized here for the first time in animal development. In contrast, AIR-1 and MZT-1 were specifically required to recruit γ-TuRC to the centrosome, but not to centrioles or to the apical ncMTOC. Surprisingly, microtubules remain robustly organized at the apical ncMTOC upon γ-TuRC and AIR-1 co-depletion, and upon depletion of other known microtubule regulators, including TPXL-1/TPX2, ZYG-9/ch-TOG, PTRN-1/CAMSAP, and NOCA-1/Ninein. However, loss of GIP-1 removed a subset of dynamic EBP-2/EB1-marked microtubules, and the remaining dynamic microtubules grew faster. Together, these results suggest that different microtubule organizing centers (MTOCs) use discrete proteins for their function, and that the apical ncMTOC is composed of distinct populations of γ-TuRC-dependent and -independent microtubules that compete for a limited pool of resources.
Asunto(s)
Centrosoma/metabolismo , Centro Organizador de los Microtúbulos/fisiología , Microtúbulos/metabolismo , Animales , Aurora Quinasa A , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/metabolismo , Proteínas Portadoras/metabolismo , Centrosoma/fisiología , Proteínas del Citoesqueleto/metabolismo , Células Epiteliales/metabolismo , Proteínas Asociadas a Microtúbulos , Centro Organizador de los Microtúbulos/metabolismo , Microtúbulos/fisiología , Proteínas Nucleares/metabolismo , Especificidad de Órganos , Tubulina (Proteína)/metabolismoRESUMEN
How mechanotransduction intersects with chemical and transcriptional factors to shape organogenesis is an important question in developmental biology. This is particularly relevant to the cardiovascular system, which uses mechanical signals from flowing blood to stimulate cytoskeletal and transcriptional responses that form a highly efficient vascular network. Using this system, artery size and structure are tightly regulated, but the underlying mechanisms are poorly understood. Here, we demonstrate that deletion of Smad4 increased the diameter of coronary arteries during mouse embryonic development, a phenotype that followed the initiation of blood flow. At the same time, the BMP signal transducers SMAD1/5/8 were activated in developing coronary arteries. In a culture model of blood flow-induced shear stress, human coronary artery endothelial cells failed to align when either BMPs were inhibited or SMAD4 was depleted. In contrast to control cells, SMAD4-deficient cells did not migrate against the direction of shear stress and increased proliferation rates specifically under flow. Similar alterations were seen in coronary arteries in vivo Thus, endothelial cells perceive the direction of blood flow and respond through SMAD signaling to regulate artery size.
Asunto(s)
Vasos Coronarios/anatomía & histología , Vasos Coronarios/citología , Células Endoteliales/citología , Células Endoteliales/metabolismo , Mecanotransducción Celular , Transducción de Señal , Proteínas Smad/metabolismo , Animales , Proteínas Morfogenéticas Óseas/metabolismo , Movimiento Celular , Polaridad Celular , Proliferación Celular , Tamaño de la Célula , Circulación Coronaria , Dilatación Patológica , Femenino , Humanos , Masculino , Ratones Endogámicos C57BL , Tamaño de los Órganos , Fosforilación , ARN Interferente Pequeño/metabolismo , Estrés MecánicoRESUMEN
Collateral arteries bridge opposing artery branches, forming a natural bypass that can deliver blood flow downstream of an occlusion. Inducing coronary collateral arteries could treat cardiac ischemia, but more knowledge on their developmental mechanisms and functional capabilities is required. Here we used whole-organ imaging and three-dimensional computational fluid dynamics modeling to define spatial architecture and predict blood flow through collaterals in neonate and adult mouse hearts. Neonate collaterals were more numerous, larger in diameter and more effective at restoring blood flow. Decreased blood flow restoration in adults arose because during postnatal growth coronary arteries expanded by adding branches rather than increasing diameters, altering pressure distributions. In humans, adult hearts with total coronary occlusions averaged 2 large collaterals, with predicted moderate function, while normal fetal hearts showed over 40 collaterals, likely too small to be functionally relevant. Thus, we quantify the functional impact of collateral arteries during heart regeneration and repair-a critical step toward realizing their therapeutic potential.
RESUMEN
Tanning, or increased epidermal pigmentation, protects organisms from ultraviolet radiation (UV)-induced damages. In this issue of Development Cell, Li et al. demonstrate a key role for a chromatin regulator-the Polycomb complex-in epidermal stem cells (EpSCs) in mediating UV-induced tanning responses and epidermal pigmentation.
Asunto(s)
Epidermis , Rayos Ultravioleta , Epigénesis GenéticaRESUMEN
In recent years, there has been increasing interest in the role of lymphatics in organ repair and regeneration, due to their importance in immune surveillance and fluid homeostasis. Experimental approaches aimed at boosting lymphangiogenesis following myocardial infarction in mice, were shown to promote healing of the heart. Yet, the mechanisms governing cardiac lymphatic growth remain unclear. Here, we identify two distinct lymphatic populations in the hearts of zebrafish and mouse, one that forms through sprouting lymphangiogenesis, and the other by coalescence of isolated lymphatic cells. By tracing the development of each subset, we reveal diverse cellular origins and differential response to signaling cues. Finally, we show that lymphatic vessels are required for cardiac regeneration in zebrafish as mutants lacking lymphatics display severely impaired regeneration capabilities. Overall, our results provide novel insight into the mechanisms underlying lymphatic formation during development and regeneration, opening new avenues for interventions targeting specific lymphatic populations.
Asunto(s)
Corazón/fisiología , Linfangiogénesis/fisiología , Vasos Linfáticos/fisiología , Miocardio/metabolismo , Regeneración/fisiología , Transducción de Señal/fisiología , Animales , Animales Modificados Genéticamente , Corazón/embriología , Corazón/crecimiento & desarrollo , Linfangiogénesis/genética , Sistema Linfático/citología , Sistema Linfático/metabolismo , Sistema Linfático/fisiología , Vasos Linfáticos/metabolismo , Ratones Noqueados , Ratones Transgénicos , Mutación , Infarto del Miocardio/fisiopatología , Regeneración/genética , Transducción de Señal/genética , Pez CebraRESUMEN
Coronary arteries (CAs) stem from the aorta at 2 highly stereotyped locations, deviations from which can cause myocardial ischemia and death. CA stems form during embryogenesis when peritruncal blood vessels encircle the cardiac outflow tract and invade the aorta, but the underlying patterning mechanisms are poorly understood. Here, using murine models, we demonstrated that VEGF-C-deficient hearts have severely hypoplastic peritruncal vessels, resulting in delayed and abnormally positioned CA stems. We observed that VEGF-C is widely expressed in the outflow tract, while cardiomyocytes develop specifically within the aorta at stem sites where they surround maturing CAs in both mouse and human hearts. Mice heterozygous for islet 1 (Isl1) exhibited decreased aortic cardiomyocytes and abnormally low CA stems. In hearts with outflow tract rotation defects, misplaced stems were associated with shifted aortic cardiomyocytes, and myocardium induced ectopic connections with the pulmonary artery in culture. These data support a model in which CA stem development first requires VEGF-C to stimulate vessel growth around the outflow tract. Then, aortic cardiomyocytes facilitate interactions between peritruncal vessels and the aorta. Derangement of either step can lead to mispatterned CA stems. Studying this niche for cardiomyocyte development, and its relationship with CAs, has the potential to identify methods for stimulating vascular regrowth as a treatment for cardiovascular disease.