Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros

Banco de datos
Tipo del documento
Asunto de la revista
País de afiliación
Intervalo de año de publicación
1.
Plant Cell Physiol ; 53(1): 53-63, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22156383

RESUMEN

The phytohormone ABA regulates seed germination and stress responses. The identification of clade A protein phosphatase type 2C (PP2C)-interacting proteins PYRABACTIN RESISTANCE 1 (PYR1)/RCAR (REGULATORY COMPONENT OF ABA RECEPTOR) and PYR1-LIKEs (PYLs) as ABA receptors has been a major advance in understanding this process. Here, our aim was to identify additional ABA response loci by suppressor screening of the jasmonate (JA)-insensitive coronatine insensitive 1-16 (coi1-16) mutant using its ABA-hypersensitive phenotype. The identification and genetic characterization of Coi1-16 Resistant to ABA (CRA) loci revealed several unknown and three previously known abi mutants (abi1, abi3 and abi4), thus providing proof-of-concept evidence for this study. The synergistic effect of ABA and JA on seed germination and cotyledon expansion was analyzed in depth and the roles of cra5 coi1-16, cra6 coi1-16, cra7 coi1-16 and cra8 coi1-16 in ABA signaling during seed germination and stress responses were functionally characterized. The cra5 coi1-16 mutant showed resistance to ABA, paclobutrazol, and abiotic stresses during germination and early developmental stages. Furthermore, the cra5 coi1-16 mutation was mapped to the short arm of chromosome V and mutants exhibited differential expression of ABA-responsive genes, suggesting that CRA5 may function as a positive regulator of ABA signaling. Interestingly, cra6 coi1-16, cra7 coi1-16 and cra8 coi1-16 mutants display similar ABA- and abiotic stress-insensitive phenotypes during seed germination and seedling establishment. Taken together, our results demonstrate a key role for CRA genes in regulating the onset of seed germination by ABA, and highlight how cra mutants can be used as powerful tools to analyze novel molecular components of ABA signaling in seeds.


Asunto(s)
Ácido Abscísico/farmacología , Proteínas de Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Sitios Genéticos/genética , Germinación/efectos de los fármacos , Mutación/genética , Plantones/crecimiento & desarrollo , Arabidopsis/efectos de los fármacos , Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Cromosomas de las Plantas/genética , Ciclopentanos/farmacología , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Ósmosis/efectos de los fármacos , Oxilipinas/farmacología , Fenotipo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Plantones/efectos de los fármacos , Plantones/genética , Semillas/efectos de los fármacos , Semillas/genética , Semillas/crecimiento & desarrollo , Transducción de Señal/efectos de los fármacos , Cloruro de Sodio/farmacología , Estrés Fisiológico/efectos de los fármacos , Estrés Fisiológico/genética
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA