MARPLE, a point-of-care, strain-level disease diagnostics and surveillance tool for complex fungal pathogens.

BACKGROUND: Effective disease management depends on timely and accurate diagnosis to guide control measures. The capacity to distinguish between individuals in a pathogen population with specific properties such as fungicide resistance, toxin production and virulence profiles is often essential to inform disease management approaches. The genomics revolution has led to technologies that can rapidly produce high-resolution genotypic information to define individual variants of a pathogen species. However, their application to complex fungal pathogens has remained limited due to the frequent inability to culture these pathogens in the absence of their host and their large genome sizes. RESULTS: Here, we describe the development of Mobile And Real-time PLant disEase (MARPLE) diagnostics, a portable, genomics-based, point-of-care approach specifically tailored to identify individual strains of complex fungal plant pathogens. We used targeted sequencing to overcome limitations associated with the size of fungal genomes and their often obligately biotrophic nature. Focusing on the wheat yellow rust pathogen, Puccinia striiformis f.sp. tritici (Pst), we demonstrate that our approach can be used to rapidly define individual strains, assign strains to distinct genetic lineages that have been shown to correlate tightly with their virulence profiles and monitor genes of importance. CONCLUSIONS: MARPLE diagnostics enables rapid identification of individual pathogen strains and has the potential to monitor those with specific properties such as fungicide resistance directly from field-collected infected plant tissue in situ. Generating results within 48 h of field sampling, this new strategy has far-reaching implications for tracking plant health threats.

Driving anger and driving behavior in adults with ADHD.

OBJECTIVE: This study assesses whether anger in the context of driving is associated with the negative driving outcomes experienced by individuals with ADHD. METHOD: ADHD adults (n = 56) complete measures of driving anger, driving anger expression, angry thoughts behind the wheel, and aggressive, risky, and crash-related behavior. Results are compared to two non-ADHD control groups: one from the same community as the ADHD sample (n = 106) but that did not complete all instruments and the other from college students (n = 432) who completed all instruments. RESULTS: ADHD participants report more driving anger and aggressive expression through the use of their vehicle and less adaptive and constructive anger expression than their non-ADHD peers. Adult ADHD drivers rate themselves as more angry, risky, and unsafe drivers and report experiencing more losses of concentration and vehicular control than college students. CONCLUSION: Results are discussed with regard to drivingrelated problems for drivers with ADHD.

Angry drivers: a test of state-trait theory.

Tested hypotheses from state-trait theory applied to anger while driving. College student drivers high in trait driving anger were compared to drivers low in trait driving anger. High anger drivers were more frequently angered in day-to-day driving (frequency hypothesis). They reported more intense anger in their most angering driving situations, when visualizing provocative driving events, and in day-to-day driving (intensity hypothesis). Driving diaries and surveys showed they engaged in more aggressive behavior and expressed their anger through more verbal, physical, and vehicular means (aggression hypothesis). They reported handling of their anger less well when visualizing provocative events and on the Adaptive/Constructive Expression scale (reduced adaptive expression hypothesis). They engaged in risky behavior (risky behavior hypothesis) and experienced more moving violations, close calls, and losses of concentration, but not more major or minor accidents (partial support for crash-related outcomes hypothesis). High anger drivers were more generally angry and impulsive and employed more negative, less controlled forms of general anger expression. Results supported state-trait theory and added to the literature showing that high anger drivers have some other psychological and behavioral characteristics that may interact negatively with anger behind the wheel.

Anger, aggression, and risky behavior: a comparison of high and low anger drivers.

This research tested hypotheses from state-trait anger theory applied to anger while driving. High and low anger drivers drove equally often and as many miles, but high anger drivers reported more frequent and intense anger and more aggression and risky behavior in daily driving, greater anger in frequently occurring situations, more frequent close calls and moving violations, and greater use of hostile/aggressive and less adaptive/constructive ways of expressing anger. In low impedance simulations, groups did not differ on state anger or aggression; however, high anger drivers reported greater state anger and verbal and physical aggression in high impedance simulations. High anger drivers drove at higher speeds in low impedance simulations and had shorter times and distances to collision and were twice as likely to crash in high impedance simulations. Additionally, high anger drivers were more generally angry. Hypotheses were generally supported, and few gender differences were noted for anger and aggression.

Driving anger and other driving-related behaviors in high and low ADHD symptom college students.

This study examined differences between college students with high and low symptoms of Attention-Deficit/Hyperactivity Disorder (ADHD). Fifty-nine introductory psychology students completed ADHD diagnostic measures and were compared on measures of driving anger and driving anger expression; accident-related, aggressive, and risky driving behaviors; general anger; and general anger expression. Results indicated high ADHD symptom college students experience more driving anger, display such anger in more hostile/aggressive ways, are more aggressive and risky on the road, experience more crash-related outcomes, are more generally angry, and tend to display anger in socially unacceptable ways. Results are discussed in regard to the understanding and treatment of ADHD.