RESUMEN
We present a unified mathematical framework for sixteen fundamental optical systems. The systems have a parallel or point source and a parallel, point, near-field or far-field target. These choices give eight configurations if we use reflectors only and take the minimum number of freeform surfaces required. Similarly, we get eight lens systems if we only use lens surfaces. The mathematical model for each system is based on Hamilton's characteristic functions and conservation of luminous flux. Some configurations lead to standard or generalized Monge-Ampère equations. The remaining systems are described by so-called generated Jacobian equations.
RESUMEN
BACKGROUND: Many cancers arise from mutations in cells within epithelial tissues. Mutations manifesting at the subcellular level influence the structure and function of the tissue resulting in cancer. Previous work has proposed how cell level properties can lead to mutant cell invasion, but has not incorporated detailed subcellular modelling RESULTS: We present a framework that allows the straightforward integration and simulation of SBML representations of subcellular dynamics within multiscale models of epithelial tissues. This allows us to investigate the effect of mutations in subcellular pathways on the migration of cells within the colorectal crypt. Using multiple models we find that mutations in APC, a key component in the Wnt signalling pathway, can bias neutral drift and can also cause downward invasion of mutant cells in the crypt. CONCLUSIONS: Our framework allows us to investigate how subcellular mutations, i.e. knockouts and knockdowns, affect cell-level properties and the resultant migration of cells within epithelial tissues. In the context of the colorectal crypt, we see that mutations in APC can lead directly to mutant cell invasion.