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1.
Photochem Photobiol Sci ; 11(6): 1099-107, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22566080

RESUMEN

The photoprocesses involved in hypericin photoinactivation of three different Candida species (C. albicans, C. parapsilosis and C. krusei) have been examined. Production of singlet oxygen from the triplet state and of superoxide from both the triplet state and the semiquinone radical anion are demonstrated. Hydrogen peroxide is formed downstream of these early events. The outcome of the photodynamic treatments is dictated by the intracellular distribution of hypericin, which is different in the three species and affects the ability of hypericin to produce the different reactive oxygen species and trigger cell-death pathways. The results are in line with the previously-observed different susceptibilities of the three Candida species to hypericin photodynamic treatments.


Asunto(s)
Candida/metabolismo , Perileno/análogos & derivados , Fármacos Sensibilizantes a Radiaciones/toxicidad , Antracenos , Benzoquinonas/metabolismo , Candida/efectos de los fármacos , Candida/efectos de la radiación , Candida albicans/efectos de los fármacos , Candida albicans/metabolismo , Candida albicans/efectos de la radiación , Peróxido de Hidrógeno/metabolismo , Cinética , Luz , Microscopía Fluorescente , Perileno/química , Perileno/toxicidad , Fármacos Sensibilizantes a Radiaciones/química , Oxígeno Singlete/metabolismo
2.
Arch Gerontol Geriatr ; 52(2): 239-42, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-20416958

RESUMEN

This study analyzed the phenotype and the chondrogenic differentiation of bone marrow-derived MSCs from old patients undergoing knee osteoarthritis or femoral fracture surgery. Twenty patients (12 females), with a mean age of 77.35±8.76 years, were studied. Ten patients suffered of knee osteoarthritis (OA) pathology and underwent surgery for arthroplasty, and the other 10 patients suffered femoral fracture. A comparative study of bone marrow-derived cultured human MSCs was carried out, and the main morphological parameters, proliferative activity and expression of surface markers were characterized. Bone marrow was obtained from the femur in all cases. The χ2-test, Mann-Whitney U-test, correlation coefficient and the Spearman test were applied. Bone marrow MSCs from old patients were able to differentiate into chondrocytic lineages. Proliferation and flow cytometry data showed no difference associated to the gender. No significant differences between the knee arthroplasty group or the femoral fracture group were found, except for higher CD49d % in MSC from fracture, and higher CD49f % in MSC from knee OA patients at passage one. MSCs from old patients suffering knee OA can be differentiated into chondrocytic lineages, and these present no differences with MSCs from femoral fracture patients.


Asunto(s)
Células de la Médula Ósea/citología , Diferenciación Celular , Condrocitos/citología , Células Madre Mesenquimatosas/citología , Anciano , Anciano de 80 o más Años , Médula Ósea , Diferenciación Celular/genética , Linaje de la Célula , Células Cultivadas , Condrogénesis/genética , Femenino , Fracturas del Fémur/patología , Citometría de Flujo , Humanos , Masculino , Persona de Mediana Edad , Osteoartritis de la Rodilla/patología , Fenotipo
3.
Atherosclerosis ; 212(1): 268-73, 2010 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-20537649

RESUMEN

OBJECTIVE: Genetic and dietary hyperhomocysteinemia has been found to decrease high density lipoproteins (HDL) and their apolipoprotein A1 (APOA1). To test the hypothesis that the presence of cysteine could normalize HDL levels in hyperhomocysteinemic cystathionine beta-synthase (Cbs)-deficient mice and that the inclusion of glycine would block this effect. METHODS: Lipids and HDL cholesterol were studied in Cbs-deficient mice and wild-type animals fed a low-methionine diet supplemented with cysteine and glycine and in Cbs-deficient mice on the same diet supplemented only with cysteine. RESULTS: Triglyceride and homocysteine levels were significantly decreased and increased, respectively in Cbs-deficient mice irrespective of treatment. However, plasma cholesterol, glucose and APOA1 were significantly decreased in homozygous Cbs-deficient mice when they received the cysteine and glycine-enriched beverage. This group of mice also showed decreased mRNA levels and increased hepatic content of APOA1 protein, the latter increase was observed in endothelial cells. A significant, inverse relationship was observed between plasma and hepatic APOA1 concentrations while a positive one was found between plasma levels of cysteine and APOA1. CONCLUSION: These data suggest an altered hepatic management of APOA1 and that cysteine may be involved in the control of this apolipoprotein at this level. Overall these findings represent a new aspect of dietary regulation of HDL at the hepatic transendothelial transport.


Asunto(s)
Apolipoproteína A-I/sangre , Biomarcadores/sangre , Cisteína/sangre , Homocisteína/sangre , Homocistinuria/sangre , Hiperhomocisteinemia/sangre , Metabolismo de los Lípidos , Hígado/metabolismo , Administración Oral , Animales , Apolipoproteína A-I/genética , Bebidas , Glucemia/metabolismo , HDL-Colesterol/sangre , Cisteína/administración & dosificación , Modelos Animales de Enfermedad , Glicina/administración & dosificación , Homocistinuria/genética , Hiperhomocisteinemia/genética , Metabolismo de los Lípidos/genética , Masculino , Ratones , Ratones Noqueados , ARN Mensajero/metabolismo , España , Triglicéridos/sangre
4.
J Orthop Res ; 27(11): 1499-507, 2009 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-19408284

RESUMEN

Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into several mesoderm lineages. They have been isolated from different tissues, such as bone marrow, adult peripheral blood, umbilical cord blood, and adipose tissue. The aim of this study was to analyze the differences in proliferation and phenotype of adipose tissue-derived MSCs from three different species, and to evaluate their capacity to differentiate into chondrocytes in vitro. A comparative study of cultured human, rabbit, and sheep mesenchymal cells from adipose tissue was carried out, and the main morphological parameters, proliferative activity, and expression of surface markers were characterized. Proliferation and flow cytometry data showed species-related differences between animal and human MSCs. Histological staining suggested that rabbit and sheep mesenchymal cells were able to differentiate into chondrocytic lineages. Human mesenchymal cells, though they could also differentiate, accomplished it with more difficulty than animal MSCs. These results could help to explain the differences in the chondrogenic capacity of sheep and rabbit MSCs when they are used as animal models compared to human mesenchymal cells in a clinical assay.


Asunto(s)
Diferenciación Celular , Condrocitos/citología , Células Madre Mesenquimatosas/citología , Tejido Adiposo/citología , Animales , Antígenos CD/biosíntesis , Linaje de la Célula , Células Cultivadas , Humanos , Fenotipo , Conejos , Ovinos
5.
Clin Immunol ; 122(1): 28-40, 2007 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-16982214

RESUMEN

The infiltration and accumulation of T cells in the rheumatoid arthritis (RA) synovial fluid (SF) are hallmarks of disease. We aimed to assess the functional relevance of FasL and of APO2L/TRAIL in the persistence of T cells in the rheumatoid SF. We have analyzed the expression of the activation markers HLA-DR and CD69 and also of the death receptor Fas/CD95 and death ligands FasL or APO2L/TRAIL in CD3+ lymphocytes from SF of 62 RA patients, together with their sensitivity to anti-Fas mAb or to rAPO2L/TRAIL, using as controls T lymphocytes present in SF of 20 patients with traumatic arthritis. T lymphocytes infiltrated in SF of RA patients have a chronically activated phenotype, but they are resistant to Fas-induced toxicity. However, they are more susceptible to rAPO2L/TRAIL than T cells in the SF of traumatic arthritis patients. In addition, we found very low amounts of bioactive FasL and APO2L/TRAIL associated with exosomes in SF from RA patients as compared with SF from traumatic arthritis patients. The observation on the sensitivity of RA SF T cells to rAPO2L could have therapeutic implications because bioactive APO2L/TRAIL could be beneficial as a RA treatment.


Asunto(s)
Artritis Reumatoide/inmunología , Líquido Sinovial/citología , Linfocitos T/inmunología , Ligando Inductor de Apoptosis Relacionado con TNF/inmunología , Antígenos CD/inmunología , Antígenos CD/metabolismo , Antígenos de Diferenciación de Linfocitos T/inmunología , Antígenos de Diferenciación de Linfocitos T/metabolismo , Complejo CD3/inmunología , Complejo CD3/metabolismo , Células Cultivadas , Proteína Ligando Fas/antagonistas & inhibidores , Proteína Ligando Fas/inmunología , Proteína Ligando Fas/metabolismo , Femenino , Citometría de Flujo , Antígenos HLA-DR/inmunología , Antígenos HLA-DR/metabolismo , Humanos , Células Jurkat , Lectinas Tipo C , Masculino , Persona de Mediana Edad , Fenotipo , Líquido Sinovial/inmunología , Linfocitos T/metabolismo , Ligando Inductor de Apoptosis Relacionado con TNF/antagonistas & inhibidores , Ligando Inductor de Apoptosis Relacionado con TNF/metabolismo , Receptor fas/inmunología , Receptor fas/metabolismo
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