Simultaneous spike-time locking to multiple frequencies.

Locking of neural firing is ubiquitously observed in the brain and occurs when neurons fire at a particular phase or in synchronization with an external signal. Here we study in detail the locking of single neurons to multiple distinct frequencies at the example of p-type electroreceptor afferents in the electrosensory system of the weakly electric fish Apteronotus leptorhynchus (brown ghost knifefish). We find that electrosensory afferents and pyramidal cells in the electrosensory lateral line lobe (ELL) lock to multiple frequencies, including the electric organ discharge (EOD) frequency, beat, and stimulus itself. We identify key elements necessary for locking to multiple frequencies, study its limits, and provide concise mathematical models reproducing our main findings. Our findings provide another example of how rate and temporal codes can coexist and complement each other in single neurons and demonstrate that sensory coding in p-type electroreceptor afferents provides a much richer representation of the sensory environment than commonly assumed. Since the underlying mechanisms are not specific to the electrosensory system, our results could provide the basis for studying multiple frequency locking in other systems.NEW & NOTEWORTHY Locking of neuronal spikes to external and internal signals is a ubiquitous neurophysiological mechanism that has been extensively studied in several brain areas and species. Using experimental data from the electrosensory system and concise mathematical models, we analyze how a single neuron can simultaneously lock to multiple frequencies. Our findings demonstrate how temporal and rate codes can complement each other and lead to rich neuronal representations of sensory signals.

Perceived timing of active head movement at different speeds.

The central nervous system must determine which sensory events occur at the same time. Actively moving the head corresponds with large changes in the relationship between the observer and the environment, sensorimotor processing, and spatiotemporal perception. Active head movement perception has been shown to be dependent on head movement velocity where participants who move their head fastest require the head to move earlier than comparison stimuli for perceived simultaneity more so than those who move their head slower. Such between-subject results cannot address whether active head movement perception changes with velocity. The present study used a within-subjects design to measure the point of subjective simultaneity (PSS) between active head movement speeds and a comparison sound stimulus to characterize the relationship between the velocity and perception of head movement onset. Our results clearly show that i) head movement perception is faster with faster head movements within-subjects, ii) active head movement onset must still precede the onset of other sensory events (average PSS: -123 ms to -52 ms; median PSS: -42 ms to -100 ms) in order to be perceived as occurring simultaneously even at the fastest speeds (average peak velocity: 76°/s-257°/s; median peak velocity 72 ms-257 ms). We conclude that head movement perception is slow, but that this delay is minimized with increased speed. These within-subject results are contrary to previous and present study between-subject results and are in agreement with literature where perception of auditory, visual and vestibular stimulus onset is less delayed with increased stimulus intensity.