Koninginin W, a New Polyketide from the Endophytic Fungus Trichoderma koningiopsis YIM PH30002.

A new compound named koninginin W (1) and four known polyketides (2-5) were isolated from endophytic fungus Trichoderma koningiopsis YIM PH30002 of Panax notoginseng. The structures of 1 - 5, including absolute configuration of 1, were elucidated on the detailed analysis of the HR-ESI-MS, 1D and 2D NMR, and X-ray crystallographic data. Koninginin W (1) presented weak antibacterial activity against Escherichia coli, Bacillus subtilis and Salmonella typhimurium.

Antimicrobial Natural Products Produced by Soil-Derived Fungus Penicillium cremeogriseum W1-1.

Ten compounds (1-10) were obtained from soil-derived Penicillium cremeogriseum W1-1 with the antimicrobial guided isolation procedure. Among them, 4 presented broad-spectrum antimicrobial activities and its preliminary mechanisms were evaluated. Compound 4 showed growth inhibition on drug-resistant pathogenic strains Escherichia coli and Candida albicans with post-contact effect (PCE), changed the morphology and membrane structure, killed cells with leakage, inhibited the growth of C. albicans by eradicating biofilms. Interestingly, the fraction containing 4 presented in vivo anti-pathogenic activities in mice, indicating this indole diterpenoid alkaloid could been used as potential antimicrobial agent.

A review of plant characteristics, phytochemistry and bioactivities of the genus Glechoma.

ETHNOPHARMACOLOGICAL RELEVANCE: Plants of the genus Glechoma have been abundantly used for thousands of years in China as folk treatments for cholelithiasis, urolithiasis, inflammation, and other conditions. AIM OF THE STUDY: This review discusses the potential application of Glechoma as an herbal medicine. The plant characteristics, ethnobotanical uses, phytochemistry, and pharmacological activities of Glechoma are summarized as a guide for phytochemical and pharmacological investigations. MATERIALS AND METHODS: Various search engines including SciFinder, Google Scholar, Scopus-Elsevier, Medline, Web of Science, and China National Knowledge Infrastructure were searched for publications on Glechoma using relevant keywords. Additionally, local records, books, and non-English journals were screened up to October 2020. RESULTS: The phytochemistry of several Glechoma plants has been systematically studied, and over one hundred different compounds have been isolated and identified. Terpenoids, flavonoids and polyphenols are the major secondary metabolites. Crude extracts and isolated compounds have been shown to exhibit various pharmacological activities including prevention of nephrolithiasis, anti-inflammatory, analgesic, anticomplement, antimicrobial, antioxidant, depigmenting, anticancer, and antiviral activities, among others. CONCLUSION: Glechoma species have been used as folk medicine to treat various diseases and have diverse biological activities, making them valuable starting materials for drug development. However, in most cases the pharmacological mechanisms, pharmacokinetics, toxicology, safety, and possible interactions with other drugs remain to be determined.

Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation.

Chaenomeles speciosa (Sweet) Nakai is a dual-purpose Chinese herbal medicine and functional food favored by minorities in Southwest China, and its fruits are used for the treatment of dyspepsia, dysentery, enteritis, and rheumatism inflammation. Some diseases may be related to microbial infection; however, it is not known how the fruits possess antimicrobial activity. We evaluated the antimicrobial bioctivity of different evaluation extracts of C. speciosa fruits by in vitro and in vivo with colony-forming unit assays, and the strongest bioactive-guided fraction was selected for column chromatography (CC), UHPLC-QTOF-MS/MS, and NMR spectroscopy to confirm the chemical constituents. The most possible antimicrobial mechanism of C. speciosa fruits was explored by metabolomics approach, fluorescence microscopy imaging, and scanning electron microscopy (SEM). Thirty compounds, which were major characteristic ions of the bioactive fraction, were determined precisely. The bioactive fraction could inhibit 18 pathogenic microorganisms, significantly reduced, especially drug-resistant bacteria, compared to ampicillin sodium salt, fluconazole, and berberine chloride form; and the minimum inhibitory concentration (MIC) or minimum fungicidal concentration (MFC) values were in the range of 0.1-1 mg/mL. The compounds 2'-methoxyaucuparin (1) and oleanolic acid (20) not only have antibacterial activity but also may have synergistic effects. Further, the bioactive fraction might inhibit the biofilm formation, enhance immunity, and restore bacterial infection damage in vitro and in vivo to kill microorganisms. The data indicated that C. speciosa fruits' major bioactive fraction enriched with triterpenes, flavonoids, and phenolics could be developed as a functional supplement for individuals to prevent and treat microbial infection.