RESUMEN
Using the self-fertilizing mangrove killifish, we characterized two mutants, shorttail (stl) and balltail (btl). These mutants showed abnormalities in the posterior notochord and muscle development. Taking advantage of a highly inbred isogenic strain of the species, we rapidly identified the mutated genes, noto and msgn1 in the stl and btl mutants, respectively, using a single lane of RNA sequencing without the need of a reference genome or genetic mapping techniques. Next, we confirmed a conserved morphant phenotype in medaka and demonstrate a crucial role of noto and msgn1 in cell sorting between the axial and paraxial part of the tail mesoderm. This novel system could substantially accelerate future small-scale forward-genetic screening and identification of mutations. Therefore, the mangrove killifish could be used as a complementary system alongside existing models for future molecular genetic studies.
Asunto(s)
Desarrollo Embrionario/genética , Fundulidae/genética , Notocorda/crecimiento & desarrollo , Cola (estructura animal)/crecimiento & desarrollo , Animales , Mapeo Cromosómico , Embrión no Mamífero , Fundulidae/crecimiento & desarrollo , Pruebas Genéticas , Genoma/genética , Mutación/genética , Notocorda/metabolismo , Fenotipo , Filogenia , Autofecundación , Cola (estructura animal)/metabolismoRESUMEN
Specimens of two undescribed and one known gonad-infecting species of Philometra Costa, 1845 (Nematoda: Philometridae) were collected in some marine fishes from off the southern coast of Iraq. Based on light and scanning electron microscopy, the following new species are described: Philometra tayeni n. sp. (males and nongravid females) from ovaries of the purple-spotted bigeye Priacanthus tayenus Richardson (Priacanthidae, Acanthuriformes), and Philometra nibeae n. sp. (males and gravid female) from the ovary of the blotched croaker Nibea maculata (Bloch et Schneider) (Sciaenidae, Acanthuriformes). Philometra tayeni is mainly characterised by a pair of postanal papillae and a V-shaped caudal mound in males and by their body lengths (2.42-2.99 mm), whereas P. nibeae differs from its gonad-infecting congeners parasitising scienids mainly based on the body length of males (2.29-2.49 mm) and their spicules (96-117 µm), absence of a pair of postanal papillae and shape of caudal mound consisting of two parts. Philometra piscaria Moravec & Justine, 2014 (males and nongravid females), a parasite of the orange-spotted grouper Epinephelus coioides (Hamilton) (Epinephelidae, Perciformes), is recorded from the Arabian (= Persian) Gulf for the first time; previously unknown females of this species are described.
Asunto(s)
Lubina , Dracunculoidea , Enfermedades de los Peces , Nematodos , Perciformes , Masculino , Femenino , Animales , Irak , Especificidad de la Especie , Gónadas/parasitología , Peces , Perciformes/parasitología , Lubina/parasitología , Microscopía Electrónica de Rastreo , Enfermedades de los Peces/parasitologíaRESUMEN
Recent examinations of some marine fishes from off the southern coast of Iraq revealed the presence of two new species of Dichelyne (Dichelyne) Jägerskiöld, 1902 (Nematoda: Cucullanidae), both intestinal parasites, namely: D. (D.) lethrini n. sp. from the smalltooth emperor Lethrinus microdon Valenciennes (Lethrinidae, Acanthuriformes) and D. (D.) iraqiensis n. sp. from the humphead snapper Lutjanus sanguineus (Cuvier) (Lutjanidae, Acanthuriformes). Specimens are described and illustrated based on light and scanning electron microscopical examinations. Dichelyne (D.) lethrini n. sp. is mainly characterised by the length of spicules (522 µm), presence of a dorsal caecum, location of deirids at the level of the posterior end of oesophagus and the post-oesophageal position of the excretory pore, number (10 pairs) and distribution of caudal papillae in the male, and the presence of two minute terminal spines on the female tail tip. Dichelyne (D.) iraqiensis n. sp. differs from the species of the subgenus Dichelyne mainly based on the absence of caudal spines, the presence of a dorsal caecum, the length of spicules (789-884 µm), the location of deirids and the excretory pore approximately at the same level, and the size of eggs (72-78 × 42-51 µm). These new species infecting marine fishes in Iraq represent new geographic records of cucullanids of the subgenus Dichelyne from fishes in marine waters off Iraq.
Asunto(s)
Ascarídidos , Enfermedades de los Peces , Nematodos , Perciformes , Animales , Femenino , Masculino , Irak , Especificidad de la Especie , Peces/parasitología , Perciformes/parasitología , Microscopía Electrónica de Rastreo , Enfermedades de los Peces/parasitologíaRESUMEN
Recent examinations of some marine fishes from off the southern coast of Iraq revealed the presence of five species of Philometra Costa, 1845 (Nematoda: Philometridae): Philometra arabiensis sp. n. (males and females) from the ovary of the shrimp scad Alepes djedaba (Forsskål) (Carangidae, Carangiformes), Philometra psettoditis Moravec, Walter et Yuniar, 2012 (females) from the body cavity of the Indian halibut Psettodes erumei (Bloch et Schneider) (Psettodidae, Pleuronectiformes), Philometra terapontis Moravec, Gopalakrishnan, Rajkumar, Saravanakumar et Kaliyamoorthy, 2011 (female) from the ovary of the jarbua terapon Terapon jarbua (Forsskål) (Terapontidae, Centrarchiformes), Philometra sp. (females) from the ovary of the Arabian blackspot threadfin Polydactylus mullani (Hora) (Polynemidae, Carangariformes) and Philometra sp. 2 of Moravec et al. (2016a) (females) from the ovary and body cavity of the bartail flathead Platycephalus indicus (Linnaeus) (Platycephalidae, Perciformes). Philometra arabiensis sp. n. is mainly characterised by the length of spicules (198-243 µm) and gubernaculum (75-99 µm), the gubernaculum/spicule length ratio (1 : 2.33-2.79), the structure of the gubernaculum distal portion and the male caudal end, and the body length of males (1.86-2.73 mm). The present findings of P. psettoditis and P. terapontis in fishes of the Arabian Gulf represent new geographical records for these parasites.
Asunto(s)
Dracunculoidea , Enfermedades de los Peces , Perciformes , Animales , Femenino , Masculino , Ovario/parasitología , Irak/epidemiología , Peces/parasitología , Perciformes/parasitología , Crustáceos , Enfermedades de los Peces/epidemiología , Enfermedades de los Peces/parasitología , Microscopía Electrónica de RastreoRESUMEN
Breast cancer is the most frequent cancer among women and causes the greatest number of cancer-related death among women all over the world. It approximately accounts for 15% of all cancer death. The human microbiota is the term applied to the aggregate of microbes that live in different habitats of living organisms 'bodies, including the gut, skin, vagina, and mouth, as well as nose, conjunctiva, pharynx, and urethra, among others. Increasing evidence is pointing to the role of the microbiome in the occurrence and development of a variety of cancers. Intestinal microbiome imbalance is related to the occurrence of gastrointestinal tumors, such as esophageal, gastric, colorectal, and gallbladder cancer. The present study aimed to identify the role of microbiota in the development of breast cancer. The women with breast cancer (n=130) in this study were in the age range of 25-75 years. The study was conducted in Kirkuk city of Iraq from September 10, 2019, to March 15, 2020. The control group included 20 women diagnosed with benign breast lesions in the age range 25-75 years, who matched the women in the patient group. Blood samples and breast tissue samples were taken from patients with breast cancer and benign breast lesions. Blood samples were examined through immunological methods, enzyme-linked immunosorbent assay (ELISA) was adopted for the detection of interleukin-19 (IL-19). Breast tissue samples were taken from breast cancer and benign breast lesions patients to isolate and identify bacteria. Based on the obtained results, only 6 out of 30 (20%) cultured breast tissue samples from women with breast cancer showed bacterial growth. In total, 4 (67%) and 2(33%) of these 6 positive cultures were Escherichia coli was and Staphylococcus aureus, respectively, and this relation was statistically significant. However, no bacterial growth was observed on the cultured breast tissue samples taken from women with benign breast lesions. Moreover, the difference between women with a positive and negative result of bacterial culture and stages of breast cancer was statistically non-significant. It is worth mentioning that 50 % of women with breast cancer and bacterial growth were within the age range of 40-49 year. The present study revealed that the difference between women with breast cancer and those with benign breast lesions was statistically highly significant according to the place of residence. In addition, the mean level of IL-19 among women with breast cancer was lower than that in women with benign breast lesions, and this relation was statistically highly significant.