Automated direct immunofluorescence analyses of skin biopsies.

BACKGROUND: Diagnosis of autoantibody- and immune complex-induced skin diseases is primarily based on direct immunofluorescence (DIF) microscopy. DIF staining is usually performed manually and, therefore, is labor intensive. The quality of immunohistochemical results considerably depends on the experience of the person conducting the tests. The novel EUROTide(™) technique in combination with the biochip-based system EUROPath represents a new technology for automation of DIF staining. METHODS: Frozen sections of previously characterized skin biopsies from bullous pemphigoid and pemphigus vulgaris patients were incubated with fluorescein-labelled anti-human IgG and complement C3c following the standard manual procedure and, for comparison, applying EUROTide/EUROPath in an automated version. RESULTS: Both, the manual and the automated procedure, detected IgG and C3c deposits in all samples. However, DIF stainings performed with EUROTide/EUROPath displayed more intense specific IF signals and distinctly less background staining. The detecting antibody could be used at a ×4 higher dilution. CONCLUSION: EUROTide/EUROPath applied in an automated system improves diagnostic accuracy and saves reagents. Larger studies in other routine laboratories may further explore the value of the EUROTide/EUROPath technology and may include comparison with other automated stainers.

The nutritional knowledge of Australian nurses.

Sound nutrition is an essential component of good health. A number of health professionals, including nurses, provide nutritional information to the community. However, little research exists which measures the nutrition knowledge of nurses in Australia. The aim of this study was to determine the nutrition knowledge of nurses in regional Victoria. A descriptive cross-sectional study design was used with 103 nurses (81% currently practicing in an acute regional hospital). The nurses answered 48 multiple choice general knowledge questions (using a valid and reliable questionnaire) and provided educational and demographic details. Each multiple choice question was scored as correct or incorrect and given the value of one point (maximum possible 48 points). The mean knowledge score for all nurses was 60.2% (SD = 8.4). Older nurses, those with more years of experience, and nurses with general training (rather than a degree) scored higher average knowledge scores. Respondents reported requests for nutrition information from patients and clients, and indicated that the most frequently used nutrition information sources were dietitians, other nurses, professional journals, books and literature from the National Heart Foundation. The nutrition knowledge score reported in this study is low to moderate by definition from previous studies using the same questionnaire. It is recommended that the Australian nursing profession determines its own nutrition knowledge standard and the nutrition knowledge needs of nurses working in particular areas of practice. Further work is required to determine the validity and reliability of an Australian knowledge instrument.