Selenium accumulation and reproduction in birds breeding downstream of a uranium mill in northern Saskatchewan, Canada.

Selenium (Se) concentrations in aquatic invertebrates and bird eggs collected along the treated effluent receiving environment of the Key Lake uranium mill in northern Saskatchewan were significantly greater than from nearby reference areas, and in some cases (e.g., eggs of common loons--Gavia immer) were higher than commonly used thresholds for adverse reproductive effects in birds (i.e., 5 µg/g dry weight in diet; 12-15 µg/g dry weight in eggs). Mean Se concentrations in tree swallow (Tachycineta bicolor) eggs reached a maximum of 13.3 µg/g dry weight at the point of treated effluent discharge and exhibited a gradient of decreasing Se concentrations with increasing distance from the effluent discharge, probably reflecting both effluent dilution and local site fidelity by nesting swallows. In some cases, high intra-clutch variability in Se concentrations in mallard (Anas platyrhynchos) and tree swallow eggs was observed in high-Se sites, suggesting that a single egg randomly sampled from a nest in an area of higher Se exposure may not be representative of Se concentrations in other eggs from the same nest. Overall, tree swallow reproductive success was similar in both exposed and reference areas.

Toxicity of methylmercury injected into eggs of thick-billed murres and arctic terns.

Mercury (Hg) has been increasing in some marine birds in the Canadian Arctic over the past several decades. To evaluate the potential reproductive impact of Hg exposure, eggs of two species of arctic-breeding seabirds, the thick-billed murre and arctic tern, were dosed with graded concentrations of methylmercury (MeHg) and artificially incubated in the laboratory to determine species differences in sensitivity. Based on the dose-response curves, the median lethal concentrations (LC(50)) for thick-billed murre and arctic tern embryos were 0.48 and 0.95 µg g(-1) Hg on a wet-weight (ww) basis, respectively. Compared with published LC(50) values for other avian species, the murres and terns had a medium sensitivity to MeHg exposure. LC(50) values were also calculated for the actual Hg concentration measured in the embryos, that is, the maternally-deposited Hg plus the injected MeHg dose. This increased the LC(50) values to 0.56 µg g(-1) Hg ww in the thick-billed murre and to 1.10 µg g(-1) Hg ww in the arctic tern. Although muscarinic acetylcholine and N-methyl-D-aspartic acid glutamate receptor levels have been correlated with increasing Hg concentrations in brains of adult birds, no significant associations were found in brain tissue of the murre or tern embryos. The incidence of gross external anatomical deformities was 4.3 % in the murre embryos and 3.6 % in the tern embryos. However, given that the eggs were taken from wild populations, it is unlikely that the deformities observed in this study were due to MeHg exposure alone.

Evidence for sex differences in mercury dynamics in double-crested cormorants.

Aquatic fish-eating birds can demethylate methylmercury in their livers. In this study, we determined whether a previously documented male bias in mercury concentration in double-crested cormorants ( Phalacrocorax auritus ) was due entirely to the depuration of mercury into eggs or might also in part be related to sex differences in methylmercury demethylation or biliary excretion capability in the liver. We found egg depuration accounted for less than a fifth of the mercury concentration difference between males and females, hence not entirely explaining the sex difference. Females had a significantly steeper slope for the negative relationship between percent methylmercury (i.e., percentage of total mercury that is methylmercury) and total mercury concentration than did males. This suggests that females have a greater capacity to demethylate methylmercury, which might be reducing the amount of methylmercury available for depuration to eggs. We also found a significant negative relationship between methylmercury concentration and liver mass for females only; thus females might also have a greater capability to excrete methylmercury compared to males. Therefore, we conclude that the male bias in mercury concentration might also result from females having a greater capability to excrete mercury compared to males.

Spatial gradients of methylmercury for breeding common loons in the Laurentian Great Lakes region.

Much of the Laurentian Great Lakes region is a mercury-sensitive landscape, in which atmospheric deposition and waterborne sources of mercury (Hg) have led to high concentrations of bioavailable methylmercury (MeHg) in predatory fish and piscivorous wildlife. Efforts since the early 1990s have established the common loon (Gavia immer) as the primary avian indicator for evaluating the exposure and effects of MeHg in North America. A regional Hg dataset was compiled from multiple loon tissue types and yellow perch (Perca flavescens), a preferred prey fish species for loons. Hg exposure in loons and perch was modeled to develop male and female loon units (MLU and FLU, respectively), standardized metrics that represent the estimated blood Hg exposure of a male or female loon for a given loon territory or water body. Using this common endpoint approach to assess loon Hg exposure, the authors demonstrate spatial trends in biotic Hg concentrations, examine MeHg availability in aquatic ecosystems of the Great Lakes region in relation to landscape-level characteristics, and identify areas with potentially significant adverse reproductive impacts to loons and other avian piscivores. Based on 8,101 MLUs, seven biological Hg hotspots were identified in the Great Lakes region. Policy-relevant applications are presented.

Is dietary mercury of neurotoxicological concern to wild polar bears (Ursus maritimus)?

Polar bears (Ursus maritimus) are exposed to high concentrations of mercury because they are apex predators in the Arctic ecosystem. Although mercury is a potent neurotoxic heavy metal, it is not known whether current exposures are of neurotoxicological concern to polar bears. We tested the hypotheses that polar bears accumulate levels of mercury in their brains that exceed the estimated lowest observable adverse effect level (20 microg/g dry wt) for mammalian wildlife and that such exposures are associated with subtle neurological damage, as determined by measuring neurochemical biomarkers previously shown to be disrupted by mercury in other high-trophic wildlife. Brain stem (medulla oblongata) tissues from 82 polar bears subsistence hunted in East Greenland were studied. Despite surprisingly low levels of mercury in the brain stem region (total mercury = 0.36 +/- 0.12 microg/g dry wt), a significant negative correlation was measured between N-methyl-D-aspartate (NMDA) receptor levels and both total mercury (r = -0.34, p < 0.01) and methylmercury (r = -0.89, p < 0.05). No relationships were observed among mercury, selenium, and several other neurochemical biomarkers (dopamine-2, gamma-aminobutyric acid type A, muscarinic cholinergic, and nicotinic cholinergic receptors; cholinesterase and monoamine oxidase enzymes). These data show that East Greenland polar bears do not accumulate high levels of mercury in their brain stems. However, decreased levels of NMDA receptors could be one of the most sensitive indicators of mercury's subclinical and early effects.

The effects of mercury on muscarinic cholinergic receptor subtypes (M1 and M2) in captive mink.

A combination of in vitro (competitive binding assays) and in vivo (tissues from animals exposed to dietary methyl mercury, MeHg) experimental procedures was employed to assess the effects of mercury (MeHg, HgCl(2)) on the two-key muscarinic cholinergic (mACh) receptor subtypes (M1, M2) in two brain regions (occipital cortex, brain stem) of captive mink (Mustela vison). In vitro, HgCl(2) and MeHg were equipotent in inhibiting [(3)H]-pirenzipine binding to the M1 receptor in the occipital cortex, but in the brain stem, MeHg was about 65x more potent than HgCl(2). For the M2 receptor, both HgCl(2) and MeHg were more potent at inhibiting [(3)H]-AFDX-384 binding in the occipital cortex than in the brain stem. Within each brain region, HgCl(2) was more potent at inhibiting [(3)H]-AFDX-384 binding than MeHg. In vivo exposure of captive mink to MeHg (0.5, 1, and 2ppm MeHg in the diet for 89 days) resulted in greater binding of radioligands to the M1 and M2 receptor in the occipital cortex, but not in the brain stem, when compared to control animals. Based on the in vitro results, we could not conclude which mACh receptor subtype or brain region was most sensitive to Hg, but the in vivo findings suggest that Hg preferentially affects mACh receptor subtype (M1 and M2) levels in the occipital cortex. By studying distinct mACh receptors, these results extend upon previous studies in laboratory rodents and wildlife that showed Hg to affect the global population of mACh receptors.

Trace element and metallothionein concentrations in seabirds from the Canadian Arctic.

Livers and kidneys were collected for five seabird species from the Canadian Arctic during the 1983 and 1991 to 1993 breeding seasons. Livers were analyzed for Cd, Hg, Pb, and Se, and kidneys were analyzed for Cd, Cu, Zn, and metallothionein (MT). Concentrations of the essential elements, Cu and Zn, were in agreement with those previously published in the literature. Thick-billed murres (Uria lomvia) from Ivujivik on the Hudson Strait/Hudson Bay coast in northwestern Quebec (Canada) had the highest mean renal concentrations of Cu, Zn, and Cd. Among the four species collected from Prince Leopold Island, northern fulmars (Fulmarus glacialis) had the highest hepatic concentrations of both Cd and Hg. The highest Se concentrations were found in northern fulmars and black-legged kittiwakes (Rissa tridactyla) from Prince Leopold Island. Hepatic Pb concentrations were low (<0.3 microg/g dry wt) in all species at all locations. Metallothionein concentrations were positively correlated with Cd and Zn for all species combined but were not correlated with Cu in any species. No significant relationships were found between MT and Cu or Zn in black guillemots (Cepphus grylle) or glaucous gulls (Larus hyperboreus). To our knowledge, this is the first report of MT concentrations and their relationships with trace metals in Arctic seabirds.

Decreased N-methyl-D-aspartic acid (NMDA) receptor levels are associated with mercury exposure in wild and captive mink.

Mercury (Hg) impairs glutamate homeostasis but little is known about its effects on the N-methyl-d-aspartic acid (NMDA) receptor. Here, we investigated NMDA receptor levels, as determined by [(3)H]-MK801 binding, in both wild and captive mink (Mustela vison) that experienced different levels of methylmercury (MeHg) exposure. Competitive in vitro binding experiments showed that inorganic Hg (HgCl(2); IC(50)=1.5-20.7 microM), but not MeHg (MeHgCl; IC(50)>320 microM), inhibited binding to the NMDA receptor in several brain regions of mink. In a survey of trapped wild mink, NMDA receptor levels in the brain were negatively correlated (p<0.005) with concentrations of total Hg (R=-0.618) and MeHg (R=-0.714). These findings were supported by a laboratory feeding study in which captive mink were exposed to dietary MeHg (0-2 ppm) for 89 days. Concentration-dependent decreases in NMDA receptor levels were found in the basal ganglia, cerebellum, brain stem and occipital cortex. These findings are of physiological and ecological concern because they demonstrate that Hg, at dietary concentrations as low as 0.1 ppm, can significantly reduce NMDA receptor levels.

Polychlorinated biphenyls, organochlorinated pesticides, and polybrominated diphenyl ethers in the cerebral cortex of wild river otters (Lontra canadensis).

We measured the levels of ortho-substituted polychlorinated biphenyls (PCB), organochlorinated pesticides (OCP), and polybrominated diphenyl ethers (PBDE) in the cerebral cortex of river otters (Lontra canadensis) trapped from Ontario and Nova Scotia between 2002 and 2004. The mean concentration of total PCBs was 70.9+/-12.1 ng/g l.w., and congeners 153, 180 and 138 accounted for nearly 60% of the sum. The mean concentration of total OCPs was 21.2+/-3.7 ng/g l.w., and hexachlorobenzene (32.6% of total) and DDE (28.1%) accounted for the majority. The mean concentration of total PBDEs was 3.2+/-0.6 ng/g l.w., and congeners 99 (44.9%), 153 (30.5%), and 100 (24.7%) were measured at the indicated percentages. There was no relationship between these residue data and concentrations of brain mercury or neurochemical receptors and enzymes as determined in earlier studies on these same animals.

Effects of environmental methylmercury on the health of wild birds, mammals, and fish.

Wild piscivorous fish, mammals, and birds may be at risk for elevated dietary methylmercury intake and toxicity. In controlled feeding studies, the consumption of diets that contained Hg (as methylmercury) at environmentally realistic concentrations resulted in a range of toxic effects in fish, birds, and mammals, including behavioral, neurochemical, hormonal, and reproductive changes. Limited field-based studies, especially with certain wild piscivorous bird species, e.g., the common loon, corroborated laboratory-based results, demonstrating significant relations between methylmercury exposure and various indicators of methylmercury toxicity, including reproductive impairment. Potential population effects in fish and wildlife resulting from dietary methylmercury exposure are expected to vary as a function of species life history, as well as regional differences in fish-Hg concentrations, which, in turn, are influenced by differences in Hg deposition and environmental methylation rates. However, population modeling suggests that reductions in Hg emissions could have substantial benefits for some common loon populations that are currently experiencing elevated methylmercury exposure. Predicted benefits would be mediated primarily through improved hatching success and development of hatchlings to maturity as Hg concentrations in prey fish decline. Other piscivorous species may also benefit from decreased Hg exposure but have not been as extensively studied as the common loon.

Methylmercury impairs components of the cholinergic system in captive mink (Mustela vison).

The effects of methylmercury (MeHg) on components of the cholinergic system were evaluated in captive mink (Mustela vison). Cholinergic parameters were measured in brain regions (occipital cortex, cerebellum, brain stem, basal ganglia) and blood (whole blood, plasma, serum) following an 89-day exposure to MeHg at dietary concentrations of 0, 0.1, 0.5, 1, and 2 ppm (n = 12 animals per treatment). There were no effects of MeHg on brain choline acetyltransferase, acetylcholine, and choline transporter. However, significantly higher densities of muscarinic cholinergic receptors, as assessed by 3H-quinuclidinyl benzilate binding, were measured in the occipital cortex (30.2 and 39.0% higher in the 1 and 2 ppm groups, respectively), basal ganglia (67.5 and 69.1% higher in the 0.5 and 1 ppm groups, respectively), and brain stem (64.4% higher in the 0.5 ppm group), compared to nonexposed controls. The calculated positive relationship between MeHg exposure and muscarinic cholinergic receptor levels in this dosing study were consistent with observations in wild mink. There were no MeHg-related effects on blood cholinesterase (ChE) activity, but ChE activity was significantly higher in the occipital cortex (17.0% in the 1 ppm group) and basal ganglia (34.1% in the 0.5 ppm group), compared to nonexposed controls. The parallel increases in muscarinic cholinergic receptor levels and ChE activity following MeHg exposure highlight the autoregulatory nature of cholinergic neurotransmission. In conclusion, these laboratory data support findings from wild mink and demonstrate that ecologically relevant exposures to MeHg (i.e., 0.5 ppm in diet) have the potential to alter the cholinergic system in specific brain regions.

Mercury exposure and reproduction in fish-eating birds breeding in the Pinchi Lake region, British Columbia, Canada.

To determine whether Hg from geologic/mining-related sources at Pinchi Lake (BC, Canada) was causing elevated Hg exposure and/or adversely affecting reproduction in fish-eating birds, breeding bald eagles (Haliaeetus leucocephalus) on Pinchi Lake and four nearby reference lakes were sampled for blood and feather Hg concentrations and monitored for reproductive success during the summers of 2000, 2001, and 2002. Eggs of red-necked grebes (Podiceps grisgena) also were collected and analyzed. Mercury levels in species at various trophic levels from Pinchi Lake averaged approximately twice those in the same species from nearby lakes combined, even in the absence of substantial new inputs of Hg to Pinchi Lake over several decades. In Pinchi Lake, Hg concentrations in blood and feathers of eagles and eggs of grebes were significantly higher than those in corresponding samples from reference lakes. However, the mean Hg concentration (0.25 microg/g wet wt) in grebe eggs from Pinchi Lake was substantially lower than accepted threshold levels for reproductive toxicity in most avian species (0.5-1.0 microg/g wet wt). Mercury concentrations in the blood of adult eagles and their chicks were highly correlated (r = 0.91, p = 0.004). Despite elevated Hg exposure in adult eagles nesting on Pinchi Lake (blood Hg concentration, 4.3-9.4 microg/ml), birds appeared to be in good body condition, did not differ significantly in terms of weight from eagles nesting on reference lakes, and exhibited no evidence of obvious abnormal behavior or lack of coordination. Eagle reproductive success and productivity on Pinchi Lake were not significantly different from those on all reference lakes combined (p = 0.483).

Effects of mercury on neurochemical receptor-binding characteristics in wild mink.

Piscivorous wildlife, such as mink (Mustela vison), routinely are exposed to mercury (Hg) in their natural environment at levels that may cause adverse behavioral outcomes. The purpose of this study was to determine if a correlation exists between neurochemical receptors and concentrations of Hg in the brains of wild mink. Specifically, receptor-binding assays were conducted to characterize the muscarinic cholinergic (mACh) and dopaminergic-2 (D2) systems in brain tissues collected from mink trapped in the Yukon Territory, Ontario, and Nova Scotia (Canada), and values were correlated with total Hg and methyl Hg (MeHg) concentrations in the brains. A significant correlation was found between Hg (total Hg and MeHg) and mACh receptor density (r = 0.546; r = 0.596, respectively) or ligand affinity (r = 0.413; r = 0.474, respectively). A significant negative correlation was found between total Hg and D2 receptor density (r = -0.340) or ligand affinity (r = -0.346). These correlations suggest that environmentally relevant concentrations of Hg may alter neurochemical function in wild mink, and that neurochemical receptor-binding characteristics can be used as a novel biomarker to assess Hg's effects on wildlife. Given the importance of the muscarinic cholinergic and dopaminergic pathways in animal behavior, further studies are required to explore the physiological and ecological significance of these findings.

Effects of cadmium ingestion on plasma and osmoregulatory hormone concentrations in male and female Pekin ducks.

Effects of ingested cadmium (Cd) on body mass and plasma, urine, salt-gland secretion, and osmoregulatory hormone concentrations were assessed in male and female Pekin ducks, Anas platyrhynchos, acclimated to 450 mM NaCl over 6 wk and then held an additional 13 wk on 300 mM NaCl (prolonged saline exposure). Groups of six birds ate diets containing 0 (control), 50 (low-Cd diet), or 300 (high Cd diet) micrograms Cd/g food. Ducks that ingested Cd, especially females, lost body mass. Cadmium ingestion did not affect salt-gland secretion concentration. Control males had higher plasma osmolality and lower relative plasma volume. These increased in both sexes during saline acclimation. The high-Cd diet suppressed the increase in plasma osmolality in both sexes, but a rise in relative plasma volume occurred only in females. Following prolonged exposure to saline, plasma osmolality and relative plasma volume were reduced in control ducks but further elevated in ducks on the high-Cd diet. Cadmium ingestion suppressed the increase in urine osmolality that occurred in control ducks during saline acclimation. Neither saline acclimation nor Cd ingestion affected plasma concentrations of arginine vasotocin or prolactin. Arginine vasotocin was not correlated with plasma osmolality; prolactin was negatively correlated with plasma osmolality, but only in males. Cadmium suppressed the increase in angiotensin II that occurred at higher salinities in control and low-Cd males. This study examined the effects of gradually increasing body cadmium content on osmotic homeostasis. Cadmium affected plasma and urine, but not salt-gland secretion, concentrations and some of these responses were sexually disparate. Cadmium did not affect osmoregulatory hormones (arginine vasotocin and prolactin) by which observed changes in plasma concentration might have been influenced.

Lead and stable lead isotope ratios in soil, earthworms, and bones of American woodcock (Scolopax minor) from eastern Canada.

A study to discriminate among different possible sources of elevated Pb exposure for American woodcock (Scolopax minor) in eastern Canada is described. Undamaged wing bones excised from young-of-the-year woodcock collected from several locations in southern Ontario, southern Quebec, New Brunswick, and Nova Scotia, Canada, along with soil and earthworm (Aporrectodea tuberculata and Lumbricus rubellus) samples from the same sites, were analyzed for total Pb, and stable Pb isotopes. Ignoring six soil samples with high (> 60 microg/g) Pb concentration from the vicinity of Montreal (QC, Canada), the mean soil-Pb concentration for all sites combined was 19 microg/g (dry wt; n = 64), with a mean 206Pb:207Pb ratio of 1.19, values typical for uncontaminated rural soils in eastern North America. In earthworms, Pb concentrations ranged from 2.4 to 865 (microg/g [dry wt], mean = 24 microg/g). Concentrations of Pb in worms and soils were positively correlated (r = 0.71; p < 0.01), and 206Pb:207Pb ratios for worms and soils were also positively correlated (r = 0.54; p < 0.05). However, most young-of-the-year woodcock with high bone-Pb accumulation (> 20 microg/g) had 206Pb:207Pb ratios substantially different from worms and soils sampled from the same areas, even though woodcock feed extensively on soil invertebrates, especially earthworms. The range of 206Pb:207Pb ratios in wing bones of woodcock with elevated Pb exposure was not consistent with exposure to environmental Pb from past gasoline combustion nor Precambrian mining wastes but was consistent with ingestion of spent Pb shotgun pellets.

Effects of methylmercury on epigenetic markers in three model species: mink, chicken and yellow perch.

We previously reported that methylmercury (MeHg) exposure is associated with DNA hypomethylation in the brain stem of male polar bears. Here, we conveniently use archived tissues obtained from controlled laboratory exposure studies to look for evidence that MeHg can disrupt DNA methylation across taxa. Brain (cerebrum) tissues from MeHg-exposed mink (Neovison vison), chicken (Gallus gallus) and yellow perch (Perca flavescens) were analyzed for total Hg levels and global DNA methylation. Tissues from chicken and mink, but not perch, were also analyzed for DNA methyltransferase (DNMT) activity. In mink we observed significant reductions in global DNA methylation in an environmentally-relevant dietary exposure group (1 ppm MeHg), but not in a higher group (2 ppm MeHg). DNMT activity was significantly reduced in all treatment groups. In chicken or yellow perch, no statistically significant effects of MeHg were observed. Dose-dependent trends were observed in the chicken data but the direction of the change was not consistent between the two endpoints. Our results suggest that MeHg can be epigenetically active in that it has the capacity to affect DNA methylation in mammals. The variability in results across species may suggest inter-taxa differences in epigenetic responses to MeHg, or may be related to differences among the exposure scenarios used as animals were exposed to MeHg through different routes (dietary, egg injection), for different periods of time (19-89 days) and at different life stages (embryonic, juvenile, adult).

Derivation of screening benchmarks for dietary methylmercury exposure for the common loon (Gavia immer): rationale for use in ecological risk assessment.

The current understanding of methylmercury (MeHg) toxicity to avian species has improved considerably in recent years and indicates that exposure to environmentally relevant concentrations of MeHg through the diet can adversely affect various aspects of avian health, reproduction, and survival. Because fish-eating birds are at particular risk for elevated MeHg exposure, the authors surveyed the available primary and secondary literature to summarize the effects of dietary MeHg on the common loon (Gavia immer) and to derive ecologically relevant toxic thresholds for dietary exposure to MeHg in fish prey. After considering the available data, the authors propose three screening benchmarks of 0.1, 0.18, and 0.4 µg g(-1) wet weight MeHg in prey fish. The lowest benchmark (0.1 µg g(-1) wet wt) is the threshold for adverse behavioral impacts in adult loons and is close to the empirically determined no observed adverse effects level for subclinical effects observed in captive loon chicks. The remaining benchmarks (0.18 and 0.4 µg g(-1) wet wt) correspond to MeHg levels in prey fish associated with significant reproductive impairment and reproductive failure in wild adult loons. Overall, these benchmarks incorporate recent findings and reviews of MeHg toxicity in aquatic fish-eating birds and provide the basis for a national ecological risk assessment for Hg and loons in Canada.

Neurochemical and electrophysiological diagnosis of reversible neurotoxicity in earthworms exposed to sublethal concentrations of CL-20.

BACKGROUND, AIM, AND SCOPE: Hexanitrohexaazaisowurtzitane (CL-20) is a relatively new energetic compound sharing some degree of structural similarity with hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), a known neurotoxic compound. Previously, we demonstrated using a noninvasive electrophysiological technique that CL-20 was a more potent neurotoxicant than RDX to the earthworm Eisenia fetida. In the present study, we investigated the effect of CL-20 exposure and subsequent recovery on muscarinic acetylcholine receptors (mAChRs) to further define the mechanism of reversible neurotoxicity of CL-20 in E. fetida. MATERIALS AND METHODS: We used a noninvasive electrophysiological technique to evaluate neurotoxicity in CL-20-treated worms, and then measured how such exposures altered levels of whole-body mAChR in the same animals. RESULTS AND DISCUSSION: A good correlation exists between these two types of endpoints. Effect on mAChR levels was most prominent at day 6 of exposure. After 7 days of recovery, both conduction velocity and mAChR were significantly restored. Our results show that sublethal concentrations of CL-20 significantly reduced mAChR levels in a concentration- and duration-dependent manner, which was accompanied with significant decreases in the conduction velocity of the medial and lateral giant nerve fibers. After 7-day post exposure recovery, worms restored both neurochemical (mAChR) and neurophysiological (conduction velocity) endpoints that were reduced during 6-day exposures to CL-20 concentrations from 0.02 to 0.22 microg/cm(2). CONCLUSIONS AND PERSPECTIVES: Our findings support the idea that CL-20 induced neurotoxic effects are reversible, and suggest that CL-20 neurotoxicity may be mediated through the cholinergic system. Future studies will investigate other neurotransmission systems such as GABA, glutamate, and monoamine. Ion channels in the nerve membrane should be examined to further define the precise mechanisms underlying CL-20 neurotoxicity.

Predicting mercury concentrations and fluxes in the water column and sediment of lakes with a limited dataset.

The purpose of the present study was to evaluate the mercury (Hg) Environmental Ratios Multimedia Ecosystem Sources (HERMES) model on two Ontario, Canada lakes (Harp and Dickie) and to include modifications to enable the model to estimate the major model input variables that tend to be missing for lakes with limited datasets. No significant differences were found for either sediment solid or bulk water total mercury (THg) when the HERMES model was applied to the two Ontario lakes, regardless of whether all available data were altered during application or only the 10 variables that tend to cause the most variation in model output (i.e., concentration of THg in atmosphere, water inflow THg concentration, water inflow rate, water volume, surface area, mean depth, suspended particulate matter concentration, settling rate of solids in water column, water temperature, and precipitation rate). Since measured sediment and water THg values do not exist for most lakes removed from industrial activities, empirical relationships were incorporated into the HERMES model framework to provide a method to double-check model output for lakes where this information is unavailable.

In vitro and whole animal evidence that methylmercury disrupts GABAergic systems in discrete brain regions in captive mink.

The effects of mercury (Hg) on key components of the GABAergic system were evaluated in discrete brain regions of captive juvenile male American mink (Neovison vison) using in vitro and in vivo (whole animal) experimental approaches. In vitro studies on cortical brain tissues revealed that inorganic Hg (HgCl(2); IC50=0.5+/-0.2microM) and methyl Hg (MeHgCl; IC50=1.6+/-0.2microM) inhibited glutamic acid decarboxylase (GAD; EC 4.1.1.15) activity. There were no Hg-related effects on [(3)H]-muscimol binding to GABA(A) receptors (IC50s>100microM). HgCl(2) (IC50=0.8+/-0.3microM) but not MeHgCl (IC50>100microM) inhibited GABA-transaminase (GABA-T; EC 2.6.1.19) activity. In a whole animal study, neurochemical indicators of GABAergic function were measured in brain regions (occipital cortex, cerebellum, brain stem, and basal ganglia) of captive mink fed relevant levels of MeHgCl (0 to 2microg/g feed, ppm) daily for 89d. No effects on GAD activity were measured. Concentration-dependent decreases in [(3)H]-muscimol binding to GABA(A) receptors and GABA-T activity were found in several brain regions, with reductions as great as 94% (for GABA(A) receptor levels) and 71% (for GABA-T activity) measured in the brain stem and basal ganglia. These results show that chronic exposure to environmentally relevant levels of MeHg disrupts GABAergic signaling. Given that GABA is the main inhibitory neurotransmitter in the mammalian nervous system, prolonged disruptions of its function may underlie the sub-clinical impacts of MeHg at relevant levels to animal health.