Freestanding Fe3O4/Ti3C2TxMXene/polyurethane composite film with efficient electromagnetic shielding and ultra-stretchable performance.

Electromagnetic pollution seriously affects the human reproductive system, cardiovascular system, people's visual system, and so on. A novel versatile stretchable and biocompatible electromagnetic interference (EMI) shielding film has been developed, which could effectively attenuate electromagnetic radiation. The EMI shielding film was fabricated with a convenient solution casting and steam annealing with 2D MXene, iron oxide nanoparticles, and soluble polyurethane. The EMI shielding effectiveness is about 30.63 dB at 8.2 GHz, based on its discretized interfacial scattering and high energy conversion efficiency. Meanwhile, the excellent tensile elongation is 30.5%, because of the sliding migration and gradient structure of the nanomaterials doped in a polymer matrix. In addition, the film also demonstrated wonderful biocompatibility and did not cause erythema and discomfort even after being attached to the arm skin over 12 h, which shows the great potential for attenuation of electromagnetic irradiation and protection of human health.

Fe3O4-doped silk fibroin-polyacrylamide hydrogel for selective and highly efficient absorption of cationic dyes pollution in water.

Overuse of organic dyes has caused serious threats to the ecosystem and human health. However, the development of high-efficient, environmentally friendly, selective, and degradable cationic dye adsorbents remains a huge challenge. In this work, a novel Fe3O4nanoparticles doped silk fibroin-polyacrylamide magnetic hybrid hydrogel (Fe3O4@SF-PAAM) was successfully fabricated by combining free radical polymerization to prepare hydrogels andin situco-precipitation to prepare nanoparticles. The obtained Fe3O4@SF-PAAM hydrogel shows strong magnetic performance with saturated magnetic of 10.2 emu mg-1and excellent swelling properties with a swelling ratio of 55867%. In addition, Fe3O4@SF-PAAM can adsorb cationic dyes such as methylene blue (MB), crystal violet, and Rhodamine B, but has no adsorption effect on anionic dyes such as methyl orange, congo red, and carmine, indicating that Fe3O4@SF-PAAM has good selective adsorption properties for cationic dyes. Interestingly, the adsorption capacity of Fe3O4@SF-PAAM was approached 2025 mg g-1for MB (MB, a typical cation dye) at 25 °C and neutral. Meanwhile, the hybrid hydrogel is reusable, the removal rate for MB is still over 90% after the five adsorption-desorption cycles. The fabricated magnetic hybrid hydrogel is a kind of a highly-efficiency and eco-friendly adsorbent and presents great potential applications in water purification and environmental protection.

Extreme Interval Entropy Based on Symbolic Analysis and a Self-Adaptive Method.

Self-adaptive methods are recognized as important tools in signal process and analysis. A signal can be decomposed into a serious of new components with these mentioned methods, thus the amount of information is also increased. In order to use these components effectively, a feature set is used to describe them. With the development of pattern recognition, the analysis of self-adaptive components is becoming more intelligent and depend on feature sets. Thus, a new feature is proposed to express the signal based on the hidden property between extreme values. In this investigation, the components are first simplified through a symbolization method. The entropy analysis is incorporated into the establishment of the characteristics to describe those self-adaptive decomposition components according to the relationship between extreme values. Subsequently, Extreme Interval Entropy is proposed and used to realize the pattern recognition, with two typical self-adaptive methods, based on both Empirical Mode Decomposition (EMD) and Empirical Wavelet Transform (EWT). Later, extreme interval entropy is applied in two fault diagnosis experiments. One experiment is the fault diagnosis for rolling bearings with both different faults and damage degrees, the other experiment is about rolling bearing in a printing press. The effectiveness of the proposed method is evaluated in both experiments with K-means cluster. The accuracy rate of the fault diagnosis in rolling bearing is in the range of 75% through 100% using EMD, 95% through 100% using EWT. In the printing press experiment, the proposed method can reach 100% using EWT to distinguish the normal bearing (but cannot distinguish normal samples at different speeds), with fault bearing in 4 r/s and in 8 r/s. The fault samples are identified only according to a single proposed feature with EMD and EWT. Therefore, the extreme interval entropy is proved to be a reliable and effective tool for fault diagnosis and other similar applications.

Identification of Key Genes Affecting Results of Hyperthermia in Osteosarcoma Based on Integrative ChIP-Seq/TargetScan Analysis.

BACKGROUND The purpose of this study was to research the effects of hyperthermia on osteosarcoma (OS) by integrating the Chromatin Immunoprecipitation with the generation sequencing (ChIP-Seq) and TargetScan analysis of heat shock transcription factor 1 (HSF1). MATERIAL AND METHODS The HSF1 ChIP-seq dataset of GSE60984 was downloaded from the Gene Expressed Omnibus (GEO) database. The HSF1-binding sites were screened by MACS2 in OS cells after 10 and 20 min of hyperthermia, and they were annotated using the ChIPseeker package. The overlapped genes were selected out when HSF1-binding sites were located in the promoter region. The Database for Annotation, Visualization, and Integrated Discovery (DAVID) was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the overlaps. The miRNA-gene pairs of the overlaps were screened out via TargetScan, and the miRNA-gene-regulated network was constructed by Cytoscape software. RESULTS 1880 and 1283 genes of promoter regions were obtained in the osteosarcoma cells after 10 and 20 min of hyperthermia, respectively, and 889 of them were overlapped. The overlapped genes were enriched in 122 GO terms and 3 KEGG pathways. There were 13 657 pairs involved in the miRNA-gene regulated network of the overlaps. CONCLUSIONS Some biomarkers were identified for OS treated with hyperthermia. Moreover, some GO terms (regulation of programmed cell death and regulation of cell death) and pathways (p53 signaling pathway, methane metabolism, and viral myocarditis) might be involved.

CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588.

BACKGROUND: Circular RNAs (circRNAs) have been confirmed to be involved in cancer pathogenesis. However, the underlying mechanism of circRNA endothelin converting enzyme 1 (circECE1) in osteosarcoma (OS) development is still not understood. METHODS: The expression levels of circECE1, microRNA-588 (miR-588) and RAB3D, member RAS oncogene family (RAB3D) were gauged by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. OS cell proliferation was assessed by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) assay. OS cell apoptosis rate and metastasis were identified by flow cytometry and transwell assay. Dual-luciferase reporter analysis and RNA immunoprecipitation (RIP) assay were performed to confirm the interactions among circECE1, miR-588 and RAB3D. Xenograft tumor models were established to explore circECE1 function in vivo. Immunohistochemistry (IHC) assay was applied to analyze RAB3D level after circECE1 knockdown. RESULTS: In OS, circECE1 expression was higher than that in normal chondroma tissues. High levels of circECE1 were positively linked to OS cell viability, proliferation, migration and invasion, and negatively linked to OS cell apoptosis rate. It was found that circECE1 was a miR-588 sponge, and miR-588 inhibitor abrogated the influence of si-circECE1 on OS cells. MiR-588 targeted RAB3D to further regulate the pathological process of OS. Moreover, silencing circECE1 blocked OS tumor growth in vivo. CONCLUSION: We elucidated the function of a novel circECE1/miR-588/RAB3D axis in OS progression.

Fabrication of 1D Ni nanochains@Zn2+ doping polypyrrole/reduced graphene oxide composites for high-performance electromagnetic wave absorption.

Nowadays, electromagnetic radiation significantly impacts the normal operation of electronic devices and poses risks to human health. To effectively address this problem, the development of composites that exhibit exceptional electrochemical wave absorption through the combination of different components holds great promise. In this study, we have successfully prepared 1D Ni nanochains@Zn2+ doping polypyrrole/reduced graphene oxide (Ni NCs@Z-P/RGO, denoted as R-x) composites using a combination of hydrothermal, solvothermal, in situ polymerization, and physical blending methods. Notably, the R-2 composite demonstrates a remarkable minimum reflection loss (RLmin) of -63.58 dB at 14.3 GHz, with a thickness of 1.61 mm. Furthermore, the R-2 composite exhibits an impressive effective absorption bandwidth (EAB) of 5.08 GHz (11.92 GHz-17 GHz) at a thickness of 1.67 mm. These outstanding performances can be attributed to the synergistic effect of the different components and a well-thought-out structural design. Moreover, to showcase the practical applicability of the material, we have conducted additional investigations on the reduction of the radar cross-sectional area (RCS). The results strongly demonstrate that the prepared composite material, when used as a coating, effectively reduces the RCS value by up to 26.6 dB m2 for R-2 at θ = 0°. The experimental methods and simulations presented in this study hold significant potential for application in wave absorption research and practical implementations. Additionally, the prepared Ni NCs@Z-P/RGO composites demonstrate feasibility as wave-absorbing materials for future utilization.

Fabrication of hierarchical reduced graphene oxide decorated with core-shell Fe3O4@polypyrrole heterostructures for excellent electromagnetic wave absorption.

The design of heterostructures with reasonable chemical composition and spatial structure is one of the effective strategies to achieve high performances electromagnetic wave (EMW) absorption. Herein, reduced graphene oxide (rGO) nanosheets decorated with hollow core-shell Fe3O4@PPy (FP) microspheres have been prepared by the combination of hydrothermal method, in situ polymerization method, directional freeze-drying and hydrazine vapor reduction. FP acting as traps can consume EMW trapped into their interior through the magnetic and dielectric losses. RGO nanosheets forming the conductive network are served as multi-reflected layers. Moreover, the impedance matching is optimized by the synergistic effect between FP and rGO. As expected, the synthetic Fe3O4@PPy/rGO (FPG) composite shows excellent EMW absorption performances with the minimum reflect loss (RLmin) of -61.20 dB at 1.89 mm and the effective absorption bandwidth (EAB) of 5.26 GHz at 1.71 mm. The excellent performances for the heterostructure are attributed to the synergistic effect of conductive loss, dielectric loss, magnetic loss, multiple reflection loss, and optimized impedance matching. This work provides a simple and effective strategy for the fabrication of lightweight, thin and high-performances EMW absorbing materials.

Circular RNA circDOCK1 contributes to osteosarcoma progression by acting as a ceRNA for miR-936 to regulate LEF1.

Background: Osteosarcoma (OS) is a serious bone malignancy that commonly occurred in humans. Recent research suggested that circular RNA (circRNA) Dedicator of cytokinesis 1 (circDOCK1, also called hsa_circ_0020378) enrolled in the tumorigenesis of osteogenic sarcoma. This subject aimed to explore the precise role and mechanism of circDOCK1 on OS progression. Methods: CircDOCK1, microRNA-936 (miR-936), and Lymphoid enhancer binding factor 1 (LEF1) levels were detected using real-time quantitative polymerase chain reaction (RT-qPCR). Cell Counting Kit-8 (CCK-8), colony formation, 5-ethynyl-2'-deoxyuridine (EdU), transwell, wound healing, and tube formation assays were used to assess OS cell proliferation, migration, invasion, and angiogenesis. Western blot analysis of protein levels of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase 2 (MMP2), MMP9, and LEF1. According to bioinformatics software (circular RNA Interactome and TargetScan) analysis, the binding between miR-936 and circDOCK1 or LEF1 was predicted, followed by verification by a dual-luciferase reporter and RNA Immunoprecipitation (RIP) assays. Results: Increased circDOCK1 and LEF1, and decreased miR-936 were found in OS tissues and cell lines. Furthermore, circDOCK1 silencing might suppress OS cell proliferation, migration, invasion, and angiogenesis in vitro. Bioinformatics analysis exhibited that circDOCK1 acted as a sponge for miR-936 and LEF1 was a downstream target of miR-936. Moreover, circDOCK1 functions through modulation of the miR-936/LEF1 axis. Conclusion: CircDOCK1 knockdown might attenuate OS cell malignant biological behaviors by regulating the miR-936/GFRA1 axis, which may highlight the diagnostic and therapeutic potential of these molecules for OS treatment.

Mitochondrial genomes of four satyrine butterflies and phylogenetic relationships of the family Nymphalidae (Lepidoptera: Papilionoidea).

The complete mitochondrial genomes (mitogenomes) of four Satyrini butterflies are newly determined and comparatively analyzed. These mitogenomes are all circular, double-stranded molecules, with the lengths of 15,194 bp (Minois dryas), 15,232 bp (Ypthima motschulskyi), 15,217 bp (Neope muirheadi) and 15,279 bp (Mycalesis francisca). Gene content and arrangement of newly sequenced mitogenomes are highly conserved and are typical of Lepidoptera. Interestingly, in M. francisca, a 48-bp insertion of macrosatellite (TA)24 is present at the trnE and trnF junction, which is rare in Lepidoptera. Among 13 protein-coding genes (PCGs) of reported Satyrinae mitogenomes, atp8 is a comparatively fast-evolving gene, and most PCGs of the four species sequenced show significant codon usage bias. Phylogenetic analyses based on the mitogenomes placed the four species sequenced in this study in Satyrini, confirming the result of morphological phylogeny. Moreover, phylogenetic analyses of the family Nymphalidae based on an expanded sampling and gene data from the GenBank and the present study show that several subtribe-level relationships in the speciose Satyrini are well supported as that previously defined by multiple-locus investigations. However, the subfamily-level relationships are not fully consistent across inference methods, and this needs further investigation based on mitogenome sequences of increased taxon sampling.

Clinical considerations for the management of cancer patients in the mitigation stage of the COVID-19 pandemic.

The ongoing outbreak of the coronavirus disease 2019 (COVID-19) has become an unprecedented threat to public health around the world. The crisis has also brought great challenges to the routine diagnosis and treatment of cancer patients, especially given the urgency and continuity of cancer care. Cancer patients need to be more prudently and individually managed to combat COVID-19. At present, the COVID-19 epidemic in some countries has moved from the outbreak phase to the remission phase. How to preserve high-quality anti-tumor therapy for cancer patients while maintaining strict prevention and control of COVID-19 is a matter of concern. Here, we summarized essential data about COVID-19 and cancer and provided the clinical recommendations for the management of cancer patients during the COVID-19 pandemic based on our practical experience and relevant literatures.

Effect of polysaccharides from Sijunzi decoction on Ca2+ related regulators during intestinal mucosal restitution.

BACKGROUND: Sijunzi decoction, a representative Chinese herbal formula of reinforcing Qi strengthening spleen, has been widely used for treating patients with gastrointestinal diseases and spleen-deficiency syndrome, however, the exact effects and mechanisms are remained unclear. HYPOTHESIS/PURPOSE: The migration of intestinal epithelial (IEC-6) cells has been demonstrated to be one of the major repair modalities during the healing of mucosal wounds. Ca2+ is the key factor in regulating cell migration. Thus, this study aimed to elucidate the potential effects and mechanisms of polysaccharides (SJZDP) extracted from Sijunzi decoction in intestinal mucosal restitution. METHOD: Cell migration was detected by scratch method with micropipette tip. Western blotting was adopted to evaluate the expression of STIM1 and STIM2 proteins. Immunofluorescence was carried out to assess the translocation of STIM1 protein. Immunoprecipitation was used to determine the levels of STIM1/TRPC1 and STIM1/STIM2 complexes. A indomethacin-induced intestinal mucosal injury rat model was applied. The content of polyamines in intestinal mucosa was detected by high-performance liquid chromatography. The morphological changes in the intestinal mucosa were observed at the end of animal experiment. RESULTS: The results showed that treatment with SJZDP significantly promoted cell migration, enhanced the level of STIM1 protein and STIM1/TRPC1 complexes, reduced the level of STIM2 protein and STIM1/STIM2 complexes. Further more, SJZDP exposure promoted the translocation of STIM1 to the plasma membrane. In vivo experiment results, the administration of SJZDP effectively reduced intestinal mucosal injury. CONCLUSION: These results revealed that SJZDP promotes intestinal epithelial restitution after wounding, presumably by regulating cellular levels of STIM1 and STIM2 differentially, stimulating the translocation of STIM1, and inducing STIM1/TRPC1 association as well as decreasing the level of STIM1/STIM2.

The first mitochondrial genome of the family Epicopeiidae and higher-level phylogeny of Macroheterocera (Lepidoptera: Ditrysia).

The Macroheterocera clade contains most of the Lepidoptera species. However, extensive comparative and phylogenetic analyses of this group using complete mitochondrial genomes (mitogenome) are limited particularly in the context of increasing macroheteroceran mitogenomes reported to date. In this study, complete mitogenome of the Epicopeia hainesii is determined as the first Epicopeiidae species with mitogenome available. The whole mitogenome is circular with 15,395 bp long, and is highly biased toward A + T nucleotides (80.6%) in nucleotide composition. Comparative analyses show that gene content and arrangement of macroheteroceran mitogenomes are generally conservative and are typical of Lepidoptera but exceptions exist. In newly sequenced mitogenome, the motif "ATACTAA" is putatively located at the end of gene nad1, rather than in intergenic sequences between trnS2 and nad1 genes routinely observed in Lepidoptera. Interestingly, multiple phylogenetic analyses recover the six macroheteroceran superfamilies as Mimallonoidea + (Drepanoidea + ((Bombycoidea + Lasiocampoidea) + (Noctuoidea + Geometroidea))), providing supports for a large-scale transcriptomic study rather than various mitogenome- and multiple-gene-based investigations. In addition, our analyses consistently place the Epicopeiidae as sister group with Geometroidea, firstly demonstrating that this family is closer with Geometroidea than Drepanoidea based on mitogenome data.

First mitochondrial genome from Yponomeutidae (Lepidoptera, Yponomeutoidea) and the phylogenetic analysis for Lepidoptera.

The complete mitochondrial genome (mitogenome) of Yponomeuta montanatus is sequenced and compared with other published yponomeutoid mitogenomes. The mitogenome is circular, 15,349 bp long, and includes the typical metazoan mitochondrial genes (13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes) and an A + T-rich region. All 13 protein-coding genes use a typical start codon ATN, the one exception being cox1, which uses CGA across yponomeutoid mitogenomes. Comparative analyses further show that the secondary structures of tRNAs are conserved, including loss of the Dihydorouidine (DHU) arm in trnS1 (AGN), but remarkable nucleotide variation has occurred mainly in the DHU arms and pseudouridine (TψC) loops. A + T-rich regions exhibit substantial length variation among yponomeutoid mitogenomes, and conserved sequence blocks are recognized but some of them are not present in all species. Multiple phylogenetic analyses confirm the position of Y. montanatus in Yponomeutoidea. However, the superfamily-level relationships in the Macroheterocera clade in Lepidoptera recovered herein show considerable difference with that recovered in previous mitogenomic studies, raising the necessity of extensive phylogenetic investigation when more mitogenomes become available for this clade.

Complete mitochondrial genome of the Pasiphila chloerata (Lepidoptera: Geometridae) and its phylogenetic implications.

In this study, the complete mitochondrial genome of Pasiphila chloerata (Mabille) was sequenced and its phylogenetic implications were investigated. The P. chloerata mitogenome is a circular, double-stranded molecule, with 15,602 bp in length. The typical 37 mitochondrial genes (13 protein-coding genes (PCGs), 22 tRNAs, and 2 rRNAs) and an A + T-rich region are included. Gene content and arrangement are highly conserved and typical of Lepidoptera. Phylogenetic analyses based on the combined 37 mitochondrial genes consistently recovered the Larentiinae and Ennominae involved are reciprocally monophyletic with the highest supports. The P. chloerata was clustered with other two members of the Larentiinae, reinforcing that of previous morphological studies.

The first mitochondrial genome from the Sterrhinae (Lepidoptera: Geometridae) and its phylogenetic implications.

In this study, the complete mitochondrial genome of a geometrid species, Idaea simplicior (Prout), was sequenced. The I. simplicior mitogenome is a circular, double-stranded molecule, with 15,950 bp in size. The typical 37 mitochondrial genes (13 PCGs, 22 tRNAs, and 2 rRNAs) and an A + T-rich region are included. Gene content and arrangement are highly conserved and typical of Lepidoptera. Interestingly, the I. simplicior mitogenome is rich in microsatellite sequences of (TA)12-18 with a scattered distribution in six intergenic regions. Phylogenetic analyses based on the combined 37 mitochondrial genes show that the Larentiinae and Ennominae are monophyletic. The Sterrhinae, which is represented by the only I. simplicior sequenced in this study, shows a closer relationship with the Larentiinae than Ennominae, which confirms previous morphological studies.

The complete mitochondrial genome of a medicinal insect, Hydrillodes repugnalis (Lepidoptera: Noctuoidea: Erebidae), and related phylogenetic analysis.

The complete mitochondrial genome (mitogenome) of an important medicinal insect Hydrillodes repugnalis (Lepidoptera: Noctuoidea) was sequenced and analyzed. The mitogenome is circular with 15,570 bp long, and shows typical gene content and arrangement. Nucleotide composition is highly biased toward A + T nucleotides (81.1%). All protein-coding genes (PCGs) initiate with canonical start codon ATN, except for cox1 being CGA. The typical stop codon TAA is used for most PCGs, while the nad4l uses the TAG, and cox1 and cox2 use incomplete termination codon T. All tRNAs have a typical clover-leaf structure, except for trnS1 (AGN) lacking the dihydrouridine arm. Comparative mitogenome analysis showed that the motif "ATGATAA" between atp8 and atp6, and the motif "ATACTAA" between trnS2 and nad1 are commonly present in noctuoid mitogenomes. In A + T-rich regions, the motif "ATAGA" and subsequent poly-T structure, the motif "ATTTA" and followed macrosatellite (AT)n element and an "A"-rich 3' end upstream of the trnM gene can be recognized across noctuoid mitogenomes. Phylogenetic analyses showed that H. repugnalis is nested into the Erebidae clade, consistently being sister to the Aganainae. Within Noctuoidea, the (Notodontidae + (Erebidae + (Nolidae + (Euteliidae + Noctuidae)))) was consistently recovered firstly based on multiple mitochondrial datasets.

Complete mitochondrial genome of the soybean leaffolder, Omiodes indicata (Lepidoptera: Pyraloidea: Crambidae), and phylogenetic analysis for Pyraloidea.

Complete mitochondrial genome (mitogenome) of the Omiodes indicata was sequenced and characterized. The circular mitogenome is 15,367bp long, including 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an A+T-rich region. Nucleotide composition is highly biased toward A+T nucleotides (81.6%). All 13 PCGs initiate with canonical start codon (ATN), except for cox1 that initiates with CGA. All tRNAs have a typical clover-leaf structure, except for trnS1 (AGN) in which the base pairs of the dihydrouridine (DHU) arm are reduced. In O. indicata, the motifs "ATGATAA" and "ATACTAA" between atp8 and atp6, trnS2 and nad1, respectively, and the motifs "ATAG" and "ATTTA" in the A+T-rich region can be identified. Comparative phylogenetic analyses based on four datasets show that the dataset including all coding positions of 13 PCGs exhibit the highest informativeness in resolving higher phylogeny of Pyraloidea. Bayesian inference (BI) and maximum likelihood (ML) analyses yield generally well-supported phylogenetic relationships among the eleven pyraloid subfamilies involved. However, the relationships among the five subfamilies (Acentropinae, Crambinae, Glaphyriinae, Schoenobiinae and Scopariinae) in ML analysis are ambiguous, which might be resolved by ample sampling in future mitogenome-based phylogenetic studies.

Exploration of the sequential gene changes in epithelial ovarian cancer induced by carboplatin via microarray analysis.

The purpose of the current study was to explore the carboplatin­induced sequential changes in gene expression and screen out key genes, which were associated with effects of carboplatin on epithelial ovarian cancer (EOC). The microarray dataset GSE13525 was downloaded from the Gene Expression Omnibus database, including 6 EOC cell samples separately treated with carboplatin at 24, 30 and 36 h (case group), and 6 samples treated with phosphate­buffered saline at the same time points (control group). A total of 3 sets of differentially expressed genes (DEGs) were respectively identified in case samples at 24, 30 and 36 h compared with the control group via the Limma package, and separately recorded as DEG­24, DEG­30 and DEG­36. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the overlapped DEGs were performed via the Database for Annotation, Visualization and Integrated Discovery. The protein­protein interaction (PPI) network was constructed and analyzed by Cytoscape software. In addition, the survival curves were drawn to illustrate the association between the expression levels of certain critical genes and the prognosis of EOC. A total of 170, 605 and 1043 DEGs were separately obtained in DEG­24 DEG­30 and DEG­36, and 110 overlaps were identified. The overlaps were enriched in 77 GO terms and 3 KEGG pathways. A total of 152 pairs were involved in the PPI network, and the abnormal expression levels (high or low) of c­Jun and cyclin B1 (CCNB1) would reduce the survival time of patients with EOC. The study indicated that c­Jun and CCNB1 may be the prognostic biomarkers of EOC treated with carboplatin, and certain pathways (such as p53 signaling pathway, cell cycle and mitogen­activation protein kinase signaling pathway) may be involved in carbo-platin­resistant EOC.

[Effect of triptorelin and an extended-interval dosing regimen in the treatment of patients with endometriosis and adenomyoma].

OBJECTIVE: To study the role of triptorelin in the treatment of patients with endometriosis, adenomyoma and fibromyoma and the effect of an extended-interval dosing regimen. METHODS: Seventy patients suffering from endometriosis, adenomyoma and fibromyoma were divided into two groups: extended-interval dosing (group E) and conventional dosing (group C). There were treated with injection of triptorelin 3.75 mg intramuscularly either every 6 weeks of totally four dose regimen (group E) or every 4 weeks of six dose regimen (group C). Comparison was made in improvement of symptoms, size of uterus and volume of tumor, as well as in serum levels of 17beta-estradiol, luteinizing hormone, and follicle-stimulating hormone. RESULTS: In each group, symptoms and tumor growth significantly improved after treatment (P < 0.05). For the patients of both groups E and C, the levels of gonadotropins and gonadal steroids were obviously reduced throughout the treatment period and up to 8 - 10 weeks after the injection of the last dose (P < 0.05). The hormonal profile of group E was similar to group C (P > 0.05). CONCLUSIONS: Gonadotropin-releasing hormone agonist is efficacious in the treatment of endometriosis and adenomyoma through reducing the serum levels of follicle-stimulating hormone, luteinizing hormone and 17beta-estradiol. The curative effect is satisfactory in most patients receiving an extended interval dosing regimen. To reduce the cost of treatment, the extended-interval dosing regimen of triptorelin should be adopted in well-equipped hospitals.

[Effects of recombinant bovine basic fibroblast growth factor on osteoblast's growth and c-fos expression].

Rat's osteoblasts cultured in vitro were stimulated by recombinant bovine basic fibroblast growth factor(rb-bFGF). After 1-2 days, the osteoblast grew long protuberance, the numbers of osteoblasts were greater than the control groups', the vitality of osteoblasts was better than that of control groups. After 1 hour, the expression of c-fos in osteoblast increased when compared with that in the control group. After the osteoblasts having been stimulated for 1-2 days, the expression of c-fos increased more conspicuously. These results show that bFGF can boost the osteoblast to grow and proliferate and can enhance the expression of c-fos gene. The increase of c-fos gene's expression may be an important step in the signal transformation process of all kinds of stimulation boosting osteoblast to proliferate.