A combination of experimental and finite element analyses of needle-tissue interaction to compute the stresses and deformations during injection at different angles.

One of the main clinical applications of the needles is its practical usage in the femoral vein catheterization. Annually more than two million peoples in the United States are exposed to femoral vein catheterization. How to use the input needles into the femoral vein has a key role in the sense of pain in post-injection and possible injuries, such as tissue damage and bleeding. It has been shown that there might be a correlation between the stresses and deformations due to femoral injection to the tissue and the sense of pain and, consequently, injuries caused by needles. In this study, the stresses and deformations induced by the needle to the femoral tissue were experimentally and numerically investigated in response to an input needle at four different angles, i.e., 30°, 45°, 60°, and 90°, via finite element method. In addition, a set of experimental injections at different angles were carried out to compare the numerical results with that of the experimental ones, namely pain score. The results revealed that by increasing the angle of injection up to 60°, the strain at the interaction site of the needle-tissue is increased accordingly while a significant falling is observed at the angle of 90°. In contrast, the stress due to injection was decreased at the region of needle-tissue interaction with showing the lowest one at the angle of 90°. Experimental results were also well confirmed the numerical observations since the lowest pain score was seen at the angle of 90°. The results suggest that the most effective angle of injection would be 90° due to a lower amount of stresses and deformations compared to the other angles of injection. These findings may have implications not only for understating the stresses and deformations induced during injection around the needle-tissue interaction, but also to give an outlook to the doctors to implement the most suitable angle of injection in order to reduce the pain as well as post injury of the patients.

Structural and tribological studies on the interaction of porcine gastric mucin with non- and cationic-modified ß-lactoglobulins.

ß-lactoglobulin (BLG) is the major whey protein with negative charges at neutral pH in aqueous media. Thus, the interaction with mucins, the major polyanionic component of mucus, is very weak due to the electrostatic repulsion between them. The present study postulates that cationization of BLG molecules may reverse the interaction characteristics between BLG and mucin from repulsive to associative. To this end, cationic-modified BLGs were prepared by grafting positively charged ethylenediamine (EDA) moieties into the negatively charged carboxyl groups on the aspartic and glutamic acid residues and compared with non-modified BLG upon mixing with porcine gastric mucin (PGM). To characterize the structural and conformational features of PGM, non/cationized BLGs, and their mixtures, various spectroscopic approaches, including zeta potential, dynamic light scattering (DLS), and circular dichroism (CD) spectroscopy were employed. Importantly, we have taken surface adsorption with optical waveguide lightmode spectroscopy (OWLS), and tribological properties with pin-on-disk tribometry at the sliding interface as the key approaches to determine the interaction nature between them as mixing PGM with polycations can lead to synergistic lubrication at the nonpolar substrate in neutral aqueous media as a result of an electrostatic association. All the spectroscopic studies and a substantial improvement in lubricity collectively supported a tenacious and associative interaction between PGM and cationized BLGs, but not between PGM and non-modified BLG. This study demonstrates a unique and successful approach to intensify the interaction between BLG and mucins, which is meaningful for a broad range of disciplines, including food science, macromolecular interactions, and biolubrication etc.

Complexation between porcine gastric mucin (PGM) and lysozyme: Influence of heat treatment of lysozyme on the tribological properties.

The influence of complexation between porcine gastric mucin (PGM) and lysozyme (LYZ) solutions (pH⁓7.0) on their lubricating properties was studied at a hydrophobic self-mated polydimethylsiloxane (PDMS) tribopair. To this end, LYZ solutions with varying heating time, namely 1hr, 3hr-, and 6hr at 90 °C, as well as unheated LYZ solution, were prepared. The lubricating capability of PGM and LYZ solutions and also their mixtures was characterized using pin-on-disk tribometry. In parallel, to precisely investigate the interaction between PGM and LYZ solutions, an array of the well-known experiments including electrophoretic-dynamic light scattering, circular dichroism spectroscopy and optical waveguide light-mode spectroscopy were employed. These experiments were utilized to elucidate the key features e.g. zeta potential, hydrodynamic diameter, conformational structure and mass adsorption. The tribometry results indicated that both PGM and unheated LYZ solutions had poor lubricating properties in the boundary lubrication regime (sliding speed lower than 10 mm/s). Mixing PGM with unheated LYZ led to a slight decrease in the friction coefficient, but no desirable lubricity was observed. An optimum slippery characteristic was achieved by incorporating 1hr heated LYZ solution into PGM one. Excellent lubricity of PGM/1hr heated LYZ may stem from surface charge compensation, tenaciously compact aggregation, unique conformational structure and considerable mass adsorption onto PDMS. This finding revealed that a strong interaction between PGM and LYZ molecules and as a result, the promising lubricating capability of PGM/LYZ mixtures, can be administered by varying heat-treatment duration of LYZ proteins.

Elastic-plastic properties of titanium and its alloys modified by fibre laser surface nitriding for orthopaedic implant applications.

Laser nitriding is one of the most promising approaches to improve wear resistance of Ti alloy surfaces and may extend the use in orthopaedic implants. In this study, three types of Ti alloys, namely alpha commercially pure Ti ("TiG2"), alpha-beta Ti-6Al-4V ("TiG5"), and beta Ti-35.5Nb-7.3Zr-5.7Ta ("ßTi"), were subjected to an open-air laser nitriding treatment. Essential elastic-plastic mechanical properties including elastic modulus, hardness, elastic energy, plasticity index, and hardness-to-elasticity ratio of the laser-treated Ti alloys were characterized using nanoindentation experiment. The results showed that the elastic modulus, hardness and elastic energy values of all Ti samples significantly increased in the nitrided layer compared to respective bare substrates for all three Ti materials. Across different Ti samples, ßTi sustained its relatively lower elastic modulus, but presented comparable hardness, elastic energy, plasticity index, as well as hardness-to-elasticity ratio in the nitrided layer compared to the other two Ti alloys. Overall, amongst three medical grade Ti alloys in this study, ßTi appeared as the most appealing candidate for joint replacement applications even solely in view of mechanical compatibility when combined with surface laser nitriding. Nevertheless, laser nitriding treatment in this study tended to cause a residual compressive stress on all Ti alloys as displayed by cracks developed in the nitrided layer and analyzed on ßTi by X-ray diffraction (XRD) and further nanoindentation tests.

A fully analytical approach to investigate the electro-viscous effect of the endothelial glycocalyx layer on the microvascular blood flow.

BACKGROUND: Recently, the glycocalyx lining the endothelial surface has emerged as a structure of fundamental importance to a wide range of phenomena that has undeniable effect on cardiovascular health and disease. With respect to the blood flow in small vessels, it has been experimentally reported that the glycocalyx layer causes additional resistance to the flow. METHODS: The hypothesis of glycocalyx resistance against the blood flow, considered as two-phase layer fluid through a small blood vessel, was theoretically evaluated. To do that, a very thin electric double layer (EDL) was considered and the fluid flow was modeled by the well-known Poisson and Boltzmann equations in micro-fluidics alongside the general Navier-Stokes equation. Finally, a complete analytical solution for this particular case was developed. RESULTS: The results confirmed the previous findings indicated that the negatively charged glycocalyx layer has no effect on the macro/micro scale blood flow. Here and in the nano-scale, slightly influence was observed and reported in this study. CONCLUSION: Moreover, more details about the thin electrically significant layer, close to the EDL, would be delineate to better recognition of electro-viscous effect caused by the endothelial glycocalyx near microvascular walls.