1.
Nat Methods
; 12(6): 519-22, 2015 Jun.
Artículo
en Inglés
| MEDLINE
| ID: mdl-25915121
RESUMEN
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.